I am Xiao Du, graduated from the University of Missouri and East China University of Science & Technology in China. I have got the bachelor degrees of Bio-engineering and Bio-science, and I maintained a high GPA in both colleges. Besides, I have worked for nanopore related research and have been listed on the paper as co-author during my undergraduate period.
- Remote Activation of a Nanopore for High-Performance Genetic Detection Using a pH Taxis-Mimicking Mechanism ANALYTICAL CHEMISTRY 2017; 89 (24): 13039–43
Nanopore electric snapshots of an RNA tertiary folding pathway
2017; 8: 1458
The chemical properties and biological mechanisms of RNAs are determined by their tertiary structures. Exploring the tertiary structure folding processes of RNA enables us to understand and control its biological functions. Here, we report a nanopore snapshot approach combined with coarse-grained molecular dynamics simulation and master equation analysis to elucidate the folding of an RNA pseudoknot structure. In this approach, single RNA molecules captured by the nanopore can freely fold from the unstructured state without constraint and can be programmed to terminate their folding process at different intermediates. By identifying the nanopore signatures and measuring their time-dependent populations, we can "visualize" a series of kinetically important intermediates, track the kinetics of their inter-conversions, and derive the RNA pseudoknot folding pathway. This approach can potentially be developed into a single-molecule toolbox to investigate the biophysical mechanisms of RNA folding and unfolding, its interactions with ligands, and its functions.
View details for DOI 10.1038/s41467-017-01588-z
View details for Web of Science ID 000414915900020
View details for PubMedID 29133841
View details for PubMedCentralID PMC5684407