Honors & Awards


  • Derek T. Turner Award (oral session), North Carolina Section of American Association of Dental Research (NC-AADR) (2018)
  • Graduate Student Transportation Grant, UNC-CH (2018)
  • NCI K00 Predoctoral to Postdoctoral Fellow Transition Award (4K00CA223019), National Institute of Health/National Cancer Institute (NIH/NCI) (2018)
  • Prestigious External Fellowship, UNC-CH (2018)
  • Dissertation Completion Fellowship, UNC-CH (2017)
  • NCI F99 Predoctoral to Postdoctoral Fellow Transition Award (1F99CA223019), National Institute of Health/National Cancer Institute (NIH/NCI) (2017)
  • NCI Predoctoral to Postdoctoral F99/K00 Fellowship Application UNC-CH Nominee, UNC-CH (2017)
  • Freedland Advanced Dental Education Fellowship, Dental Foundation of North Carolina (2016)
  • Derek T. Turner Award Finalist (poster session), NC-AADR (2014)
  • Doctoral Merit Assistantship, UNC-CH (2013)
  • 1st Class Graduate Scholarships for Outstanding Academic Performance, Wuhan University (2010-2013)
  • Undergraduate Research Award, Wuhan University (2008)
  • “Heraeus Kulzer” Honor Scholarship for Excellent Dental Students, Heraeus Kulzer (2008)
  • 1st Prize for pioneer of college student's social activity, Hubei Province, China (2007)
  • Excellent League Member, Wuhan University (2007)
  • Undergraduate Scholarships for Outstanding Academic Performance, Wuhan university (2005-2010)

Boards, Advisory Committees, Professional Organizations


  • Member, American Dental Education Association (2018 - Present)
  • Member, Society for Leukocyte Biology (2015 - Present)
  • Member, American Association of Immunologists (2017 - Present)
  • Member, International Association for Dental Research (2015 - Present)

Professional Education


  • Doctor of Philosophy, Wuhan University (2015)
  • Doctor of Dental Surgery, Wuhan University (2015)
  • Doctor of Philosophy, University of North Carolina, Chapel Hill (2018)
  • Bachelor of Medicine, Wuhan University (2010)

Patents


  • Ning Cheng. "United States Patent OTD: 15-0080. Attorney Docket N. 5470-787PR Methods and compositions for inducing an immune response.", UNC-CH, Sep 19, 2016

All Publications


  • A robust microparticle platform for a STING-targeted adjuvant that enhances both humoral and cellular immunity during vaccination. Journal of controlled release : official journal of the Controlled Release Society Junkins, R. D., Gallovic, M. D., Johnson, B. M., Collier, M. A., Watkins-Schulz, R., Cheng, N., David, C. N., McGee, C. E., Sempowski, G. D., Shterev, I., McKinnon, K., Bachelder, E. M., Ainslie, K. M., Ting, J. P. 2018; 270: 1–13

    Abstract

    Most FDA-approved adjuvants for infectious agents boost humoral but not cellular immunity, and have poorly-understood mechanisms. Stimulator of interferon genes (STING, also known as MITA, MPYS, or ERIS) is an exciting adjuvant target due to its role in cyclic dinucleotide (CDN)-driven anti-viral immunity; however, a major hindrance is STING's cytosolic localization which requires intracellular delivery of its agonists. As a result, STING agonists administered in a soluble form have elicited suboptimal immune responses. Delivery of STING agonists via particle platforms has proven a more successful strategy, but the opportunity for improved formulations and bioactivity remains. In this study we evaluated the adjuvant activity of the potent STING agonist, CDN 3'3'-cGAMP (cGAMP), encapsulated in acid-sensitive acetalated dextran (Ace-DEX) polymeric microparticles (MPs) which passively target antigen-presenting cells for intracellular release. This formulation was superior to all particle delivery systems evaluated and maintained its bioactivity following a sterilizing dose of gamma irradiation. Compared to soluble cGAMP, the Ace-DEX cGAMP MPs enhanced type-I interferon responses nearly 1000-fold in vitro and 50-fold in vivo, caused up to a 104-fold boost in antibody titers, increased Th1-associated responses, and expanded germinal center B cells and memory T cells. Furthermore, the encapsulated cGAMP elicited no observable toxicity in animals and achieved protective immunity against a lethal influenza challenge seven months post-immunization when using CDN adjuvant doses up to 100-fold lower than previous reports. For these reasons, Ace-DEX MP-encapsulated cGAMP represents a potent vaccine adjuvant of humoral and cellular immunity.

    View details for DOI 10.1016/j.jconrel.2017.11.030

    View details for PubMedID 29170142

    View details for PubMedCentralID PMC5808851

  • A nanoparticle-incorporated STING activator enhances antitumor immunity in PD-L1-insensitive models of triple-negative breast cancer. JCI insight Cheng, N., Watkins-Schulz, R., Junkins, R. D., David, C. N., Johnson, B. M., Montgomery, S. A., Peine, K. J., Darr, D. B., Yuan, H., McKinnon, K. P., Liu, Q., Miao, L., Huang, L., Bachelder, E. M., Ainslie, K. M., Ting, J. P. 2018; 3 (22)

    Abstract

    Triple-negative breast cancer (TNBC) has few therapeutic options, and alternative approaches are urgently needed. Stimulator of IFN genes (STING) is becoming an exciting target for therapeutic adjuvants. However, STING resides inside the cell, and the intracellular delivery of CDNs, such as cGAMP, is required for the optimal activation of STING. We show that liposomal nanoparticle-delivered cGAMP (cGAMP-NP) activates STING more effectively than soluble cGAMP. These particles induce innate and adaptive host immune responses to preexisting tumors in both orthotopic and genetically engineered models of basal-like TNBC. cGAMP-NPs also reduce melanoma tumor load, with limited responsivity to anti-PD-L1. Within the tumor microenvironment, cGAMP-NPs direct both mouse and human macrophages (M), reprograming from protumorigenic M2-like phenotype toward M1-like phenotype; enhance MHC and costimulatory molecule expression; reduce M2 biomarkers; increase IFN-γ-producing T cells; augment tumor apoptosis; and increase CD4+ and CD8+ T cell infiltration. Activated T cells are required for tumor suppression, as their depletion reduces antitumor activity. Importantly, cGAMP-NPs prevent the formation of secondary tumors, and a single dose is sufficient to inhibit TNBC. These data suggest that a minimal system comprised of cGAMP-NP alone is sufficient to modulate the tumor microenvironment to effectively control PD-L1-insensitive TNBC.

    View details for DOI 10.1172/jci.insight.120638

    View details for PubMedID 30429378

    View details for PubMedCentralID PMC6302949

  • The Inhibitory Innate Immune Sensor NLRP12 Maintains a Threshold against Obesity by Regulating Gut Microbiota Homeostasis. Cell host & microbe Truax, A. D., Chen, L., Tam, J. W., Cheng, N., Guo, H., Koblansky, A. A., Chou, W. C., Wilson, J. E., Brickey, W. J., Petrucelli, A., Liu, R., Cooper, D. E., Koenigsknecht, M. J., Young, V. B., Netea, M. G., Stienstra, R., Sartor, R. B., Montgomery, S. A., Coleman, R. A., Ting, J. P. 2018; 24 (3): 364–78.e6

    Abstract

    In addition to high-fat diet (HFD) and inactivity, inflammation and microbiota composition contribute to obesity. Inhibitory immune receptors, such as NLRP12, dampen inflammation and are important for resolving inflammation, but their role in obesity is unknown. We show that obesity in humans correlates with reduced expression of adipose tissue NLRP12. Similarly, Nlrp12-/- mice show increased weight gain, adipose deposition, blood glucose, NF-κB/MAPK activation, and M1-macrophage polarization. Additionally, NLRP12 is required to mitigate HFD-induced inflammasome activation. Co-housing with wild-type animals, antibiotic treatment, or germ-free condition was sufficient to restrain inflammation, obesity, and insulin tolerance in Nlrp12-/- mice, implicating the microbiota. HFD-fed Nlrp12-/- mice display dysbiosis marked by increased obesity-associated Erysipelotrichaceae, but reduced Lachnospiraceae family and the associated enzymes required for short-chain fatty acid (SCFA) synthesis. Lachnospiraceae or SCFA administration attenuates obesity, inflammation, and dysbiosis. These findings reveal that Nlrp12 reduces HFD-induced obesity by maintaining beneficial microbiota.

    View details for DOI 10.1016/j.chom.2018.08.009

    View details for PubMedID 30212649

    View details for PubMedCentralID PMC6161752

  • The Toll-Like Receptor 2/6 Agonist, FSL-1 Lipopeptide, Therapeutically Mitigates Acute Radiation Syndrome. Scientific reports Kurkjian, C. J., Guo, H., Montgomery, N. D., Cheng, N., Yuan, H., Merrill, J. R., Sempowski, G. D., Brickey, W. J., Ting, J. P. 2017; 7 (1): 17355

    Abstract

    Risks of radiation exposure from nuclear incidents and cancer radiotherapy are undeniable realities. These dangers urgently compel the development of agents for ameliorating radiation-induced injuries. Biologic pathways mediated by myeloid differentiation primary response gene 88 (MyD88), the common adaptor for toll-like receptor (TLR) and Interleukin-1 receptor signaling, are critical for radioprotection. Treating with agonists prior to radiation enhances survival by activating TLR signaling, whereas radiomitigating TLR-activating therapeutics given after exposure are less defined. We examine the radiomitigation capability of TLR agonists and identify one that is superior for its efficacy and reduced toxic consequences compared to other tested agonists. We demonstrate that the synthetic TLR2/6 ligand Fibroblast-stimulating lipopeptide (FSL-1) substantially prolongs survival in both male and female mice when administered 24 hours after radiation and shows MyD88-dependent function. FSL-1 treatment results in accelerated hematopoiesis in bone marrow, spleen and periphery, and augments systemic levels of hematopoiesis-stimulating factors. The ability of FSL-1 to stimulate hematopoiesis is critical, as hematopoietic dysfunction results from a range of ionizing radiation doses. The efficacy of a single FSL-1 dose for alleviating radiation injury while protecting against adverse effects reveals a viable radiation countermeasures agent.

    View details for DOI 10.1038/s41598-017-17729-9

    View details for PubMedID 29230065

    View details for PubMedCentralID PMC5725477

  • Arp2/3 Complex Is Required for Macrophage Integrin Functions but Is Dispensable for FcR Phagocytosis and In Vivo Motility. Developmental cell Rotty, J. D., Brighton, H. E., Craig, S. L., Asokan, S. B., Cheng, N., Ting, J. P., Bear, J. E. 2017; 42 (5): 498–513.e6

    Abstract

    The Arp2/3 complex nucleates branched actin, forming networks involved in lamellipodial protrusion, phagocytosis, and cell adhesion. We derived primary bone marrow macrophages lacking Arp2/3 complex (Arpc2-/-) and directly tested its role in macrophage functions. Despite protrusion and actin assembly defects, Arpc2-/- macrophages competently phagocytose via FcR and chemotax toward CSF and CX3CL1. However, CR3 phagocytosis and fibronectin haptotaxis, both integrin-dependent processes, are disrupted. Integrin-responsive actin assembly and αM/β2 integrin localization are compromised in Arpc2-/- cells. Using an in vivo system to observe endogenous monocytes migrating toward full-thickness ear wounds we found that Arpc2-/- monocytes maintain cell speeds and directionality similar to control. Our work reveals that the Arp2/3 complex is not a general requirement for phagocytosis or chemotaxis but is a critical driver of integrin-dependent processes. We demonstrate further that cells lacking Arp2/3 complex function in vivo remain capable of executing important physiological responses that require rapid directional motility.

    View details for DOI 10.1016/j.devcel.2017.08.003

    View details for PubMedID 28867487

    View details for PubMedCentralID PMC5601320

  • The effect of mesoporous bioglass on osteogenesis and adipogenesis of osteoporotic BMSCs. Journal of biomedical materials research. Part A Wu, T., Cheng, N., Xu, C., Sun, W., Yu, C., Shi, B. 2016; 104 (12): 3004–14

    Abstract

    This study evaluated the effect of mesoporous bioglass (MBG) dissolution on the differentiation of bone marrow mesenchymal stem cells (BMSCs) derived from either sham control or ovariectomized (OVX) rats. MBG was fabricated by evaporation-induced self-assembly method. Cell proliferation was tested by Cell Counting Kit-8 assay, and cytoskeletal morphology was observed by fluorescence microscopy. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and activity, Alizarin Red staining, while adipogenic differentiation was assessed by Oil Red-O staining. Quantitative real-time PCR and Western blot analysis were taken to evaluate the expression of runt-related transcription factor 2 (Runx2) and proliferator-activated receptor-γ (PPARγ). We found that MBG dissolution (0, 25, 50, 100, 200 µg/mL) was nontoxic to BMSCs growth. Sham and OVX BMSCs exhibited the highest ALP activity in 50 µg/mL of MBG osteogenic dissolution, except that sham BMSCs in 100 µg/mL showed the highest ALP activity on day 14. Runx2 was significantly upregulated after 100 µg/mL of MBG stimulation in sham and OVX BMSCs for 7 and 14 days, except that 25 µg/mL showed highest upregulation effect on OVX BMSCs at day 7. PPARγ was downregulated after MBG stimulation. The protein level of Runx2 from the sham BMSCs group was significantly upregulated after lower doses (25 and 50 µg/mL) of MBG stimulation, whereas PPARγ was downregulated in the sham and OVX BMSCs group. Thus, both the osteogenic and adipogenic abilities of BMSCs were damaged under OVX condition. Moreover, lower concentration of MBG dissolution can promote osteogenesis but inhibit adipogenesis of the sham and OVX BMSCs. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3004-3014, 2016.

    View details for DOI 10.1002/jbm.a.35841

    View details for PubMedID 27449696

    View details for PubMedCentralID PMC5995467

  • PNIPAAM modified mesoporous hydroxyapatite for sustained osteogenic drug release and promoting cell attachment. Materials science & engineering. C, Materials for biological applications Wu, T., Tan, L., Cheng, N., Yan, Q., Zhang, Y. F., Liu, C. J., Shi, B. 2016; 62: 888–96

    Abstract

    This work presented a sustained release system of simvastatin (SIM) based on the mesoporous hydroxyapatite (MHA) capped with poly(N-isopropylacrylamide) (PNIPAAM). The MHA was prepared by using cetyltrimethylammonium bromide (CTAB) as a template and the modified PNIPAAM layer on the surface of MHA was fabricated through surface-initiated atom transfer radical polymerization (SI-ATRP). The SIM loaded MHA-PNIPAAM showed a sustained release of SIM at 37 °C over 16 days. The bone marrow mesenchymal stem cell (BMSC) proliferation was assessed by cell counting kit-8 (CCK-8) assay, and the osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity and Alizarin Red staining. The release profile showed that the release of SIM from MHA-SIM-PNIPAAM lasted 16 days and the cumulative amount of released SIM was almost seven-fold than MHA-SIM. Besides, SIM loaded MHA-PNIPAAM exhibited better performance on cell proliferation, ALP activity, and calcium deposition than pure MHA due to the sustained release of SIM. The quantity of ALP in MHA-SIM-PNIPAAM group was more than two fold than pure MHA group at 7 days. Compared to pure MHA, better BMSC attachment on PNIPAAM modified MHA was observed using fluorescent microscopy, indicating the better biocompatibility of MHA-PNIPAAM.

    View details for DOI 10.1016/j.msec.2016.01.012

    View details for PubMedID 26952496

    View details for PubMedCentralID PMC5995466

  • Effect of retinoic acid on the function of lipopolysaccharide-stimulated bone marrow stromal cells grown on titanium surfaces. Inflammation research : official journal of the European Histamine Research Society ... [et al.] Yan, Q., Li, Y., Cheng, N., Sun, W., Shi, B. 2015; 64 (1): 63–70

    Abstract

    This study aimed to evaluate the effect of all-trans retinoic acid (atRA) on suppressing the inflammatory response and promoting the osteoblastic differentiation of bone marrow stromal cells (BMSCs) on titanium in a lipopolysaccharide (LPS)-induced microenvironment.BMSCs were divided into four groups and treated with LPS (1 μg/mL), atRA (1 nmol/L), LPS + atRA, or left untreated. Cells were then cultured on titanium surfaces and cell function compared. BMSC proliferation and osteoblastic differentiation were assessed using the MTT assay, alkaline phosphatase (ALP) activity, alizarin red staining, and quantitative real-time polymerase chain reaction (RT-PCR). Expression levels of inflammatory factors were measured by quantitative RT-PCR and enzyme-linked immunosorbent assay.Increased mineralized nodule formation, ALP activity, osteocalcin, and osteopontin expression levels were detected in LPS + atRA-treated BMSCs after osteogenic induction, when compared with LPS-treated cells. In addition, the high levels of tumor necrosis factor-α, interleukin-1β, and receptor activator of nuclear factor-κ B ligand (RANKL) expression induced by LPS were inhibited after treatment with atRA.Our results showed the effects of atRA on suppressing inflammatory responses and promoting osteoblastic differentiation of BMSCs on titanium in an LPS-induced microenvironment. This indicates the potential therapeutic value of atRA for treating peri-implants inflammatory disease.

    View details for DOI 10.1007/s00011-014-0784-7

    View details for PubMedID 25403801

    View details for PubMedCentralID PMC5995472

  • Effect of decreased implant healing time on bone (re)modeling adjacent to plateaued implants under functional loading in a dog model. Clinical oral investigations Dai, J., Cheng, N., Miron, R. J., Shi, B., Cheng, X., Zhang, Y. 2014; 18 (1): 77–86

    Abstract

    The purpose of this study was to evaluate the effect of early functional loading to plateaued implants on bone formation and implant stability in a dog model.Early loading (EL), nonloading control, and delayed loading (DL) groups were compared using six beagle dogs under functional loading. The Periotest® values were measured dynamically for 6 weeks. Peri-implant bone architecture was evaluated qualitatively by microcomputed tomography (μCT) and analyzed quantitatively by mineral apposition rates (MAR), bone-to-implant contact (BIC), and bone volumes (BV/TV) after the euthanasia at 3 and 6 weeks after loading.The EL implants showed poor stability at 1 week, but greater stability at 2 and 4 weeks after loading compared to DL implants. There was no significant difference between MAR of EL and unloaded implants at both time intervals. The EL implants displayed a significantly higher MAR when compared to DL implants at 3-5 weeks. A significantly higher BIC for the DL group was observed when compared to the EL group at 3 weeks following loading, however at 6 weeks; no significant difference between these groups was observed. The EL group gained a higher BIC than the no-treatment control group at 6 weeks.For plateaued implant, the decreased healing time (1 week) displays a positive effect on peri-implant bone (re)modeling under functional loading during the early phase.The early application of functional loading on plateaued implants can be used clinically to shorten the course of treatment and improve esthetics.

    View details for DOI 10.1007/s00784-013-0929-z

    View details for PubMedID 23377840

    View details for PubMedCentralID PMC5995475

  • The osteogenic potential of mesoporous bioglasses/silk and non-mesoporous bioglasses/silk scaffolds in ovariectomized rats: in vitro and in vivo evaluation. PloS one Cheng, N., Wang, Y., Zhang, Y., Shi, B. 2013; 8 (11): e81014

    Abstract

    Silk-based scaffolds have been introduced to bone tissue regeneration for years, however, their local therapeutic efficiency in bone metabolic disease condition has been seldom reported. According to our previous report, mesoporous bioactive glass (MBG)/silk scaffolds exhibits superior in vitro bioactivity and in vivo osteogenic properties compared to non-mesoporous bioactive glass (BG)/silk scaffolds, but no information could be found about their efficiency in osteoporotic (OVX) environment. This study investigated a biomaterial-based approach for improving MSCs behavior in vitro, and accelerating OVX defect healing by using 3D BG/silk and MBG/silk scaffolds, and pure silk scaffolds as control. The results of SEM, CCK-8 assay and quantitative ALP activity showed that MBG/silk scaffolds can improve attachment, proliferation and osteogenic differentiation of both O-MSCs and sham control. In vivo therapeutic efficiency was evaluated by μCT analysis, hematoxylin and eosin staining, safranin O staining and tartrate-resistant acid phosphatase, indicating accelerated bone formation with compatible scaffold degradation and reduced osteoclastic response of defect healing in OVX rats after 2 and 4 weeks treatment, with a rank order of MBG/silk > BG/silk > silk group. Immunohistochemical markers of COL I, OPN, BSP and OCN also revealed that MBG/silk scaffolds can better induce accelerated collagen and non-collagen matrix production. The findings of this study suggest that MBG/silk scaffolds provide a better environment for cell attachment, proliferation and differentiation, and act as potential substitute for treating local osteoporotic defects.

    View details for DOI 10.1371/journal.pone.0081014

    View details for PubMedID 24265840

    View details for PubMedCentralID PMC3827187

  • Porous CaP/silk composite scaffolds to repair femur defects in an osteoporotic model. Journal of materials science. Materials in medicine Cheng, N., Dai, J., Cheng, X., Li, S., Miron, R. J., Wu, T., Chen, W., Zhang, Y., Shi, B. 2013; 24 (8): 1963–75

    Abstract

    The most common complication for patients with postmenopausal osteoporosis is bone-related defects and fractures. While routine medication has a high probability of undesirable side effects, new approaches have aimed to develop regeneration procedures that stimulate new bone formation while reversing bone loss. Recently, we have synthesized a new hybrid CaP/silk scaffold with a CaP-phase distribution and pore architecture better suited to facilitate cell differentiation and bone formation. The aim of the present study was to compare the involved remodeling process and therapeutic effect of porous CaP/silk composite scaffolds upon local implantation into osteoporotic defects. Wistar rats were used to induce postmenopausal osteoporotic model by bilateral ovariectomy. The pure silk and hybrid CaP/silk scaffolds were implanted into critical sized defects created in distal femoral epiphysis. After 14 and 28 days, the in vivo osteogenetic efficiency was evaluated by μCT analysis, hematoxylin and eosin staining, Safranin O staining, tartrate-resistant acid phosphatase staining, and immunohistochemical assessment. Animals with or without critical-sized defects were used as drill or blank controls, respectively. The osteoporotic defect model was well established with significantly decreased μCT parameters of BV/TV, Tb.N and increased Tb.Sp, porosity, combined with changes in histological observations. During the healing process, the critical-sized drill control defects failed to regenerate appreciable bone tissue, while more significantly increased bone formation and mineralization with dynamic scaffold degradation and decreased osteoclastic bone resorption could be detected within defects with hybrid CaP/silk scaffolds compared to pure silk scaffolds.

    View details for DOI 10.1007/s10856-013-4945-y

    View details for PubMedID 23674058

    View details for PubMedCentralID PMC5995474

  • Delivery of PDGF-B and BMP-7 by mesoporous bioglass/silk fibrin scaffolds for the repair of osteoporotic defects. Biomaterials Zhang, Y., Cheng, N., Miron, R., Shi, B., Cheng, X. 2012; 33 (28): 6698–6708

    Abstract

    Osteoporosis is a chronic disease affecting millions of people worldwide caused by an imbalance between bone-forming osteoblasts and bone-resorbing osteoclasts. Despite recent developments in pharmacological agents to prevent osteoporotic-related fractures, much less attention has been placed on the repair of bone defects following fracture. Critical to this process is the recruitment of mesenchymal stem cells (MSCs) to defect sites by growth factors. One method which has been effective for the sustained release of growth factors is that of gene therapy. The aim of the present study was to investigate newly developed mesoporous bioglass/silk fibrin scaffolds containing adPDGF-b and adBMP-7 into osteoporotic critical-sized femur defects in ovariectomised rats following treatment periods of 2 and 4 weeks. In vivo osteogenetic efficiency evaluated by μ-CT analysis, hematoxylin and eosin staining, and immunohistochemical (type I collagen, osteopontin and BSP) revealed significantly new bone formation in defects containing adenovirus for both PDGF-b and BMP-7 when compared to scaffolds alone and scaffolds containing BMP-7. TRAP-positive staining also demonstrated the ability for these scaffolds to be degraded over time and initiate bone turnover/remodeling. Although the use of gene therapy for clinical applications is still in its infancy, results from the present study demonstrate their potent ability to recruit mesenchymal progenitor cells through sustained release of PDGF-b and BMP-7 which may be beneficial for patients suffering from osteoporotic-related fractures.

    View details for DOI 10.1016/j.biomaterials.2012.06.021

    View details for PubMedID 22763224

    View details for PubMedCentralID PMC5995476

  • Randomized controlled trial on fluoride varnish application for treatment of white spot lesion after fixed orthodontic treatment. Clinical oral investigations Du, M., Cheng, N., Tai, B., Jiang, H., Li, J., Bian, Z. 2012; 16 (2): 463–68

    Abstract

    The purpose of this study was to determine the efficacy of fluoride varnish (5% sodium fluoride, Duraphat(®), Colgate) in reverting white spot lesions (WSLs) after fixed orthodontic treatment. This study was a randomized, parallel group, controlled clinical trial. Using saline solution as control, 110 participants (mean age ± standard deviation: 16.6 ± 3.2 years) ranging from 12 to 22 years old were randomly assigned to either the test group (group 1) or the control group (group 2). Application of fluoride varnish or saline was applied onto tooth surfaces with WSLs every month during the first 6 months after debonding. The labial (buccal) surfaces of the teeth were assessed by the use of a DIAGNOdent pen (DD) at the baseline, 3- and 6-month follow-up visits. After 6 months, 96 subjects with a total of 209 study teeth (47 subjects, 104 teeth in group 1; 49 subjects, 105 teeth in group 2) remained. The WSLs had a mean DD reading at baseline of 17.66 ± 5.36 in group 1 and 16.19 ± 5.70 in group 2, which decreased by 5.78 and 2.44, respectively, at the 3-month follow-up visit and decreased by 7.56 and 3.09, respectively, at the 6-month follow-up visit. The mean baseline DD readings in the two groups were similar (t test, P > 0.05). There was statistically significant differences between the mean DD readings of the two groups at the 3-month (P < 0.05) and at the 6-month follow-up visits (P < 0.01). Topical fluoride varnish application is effective in reversing WSLs after debonding and should be advocated as a routine caries prevention measure after orthodontic treatment.

    View details for DOI 10.1007/s00784-011-0520-4

    View details for PubMedID 21331637

    View details for PubMedCentralID PMC5995463

  • Current progress on Zirconia Implant. Journal of Oral Science Research Cheng, N., Shi, B. 2011; 27 (12): 1115-22
  • Desmoplastic ameloblastoma - A review. Oral oncology Sun, Z. J., Wu, Y. R., Cheng, N., Zwahlen, R. A., Zhao, Y. F. 2009; 45 (9): 752–59

    Abstract

    Among the ameloblastomas, the desmoplastic variation is rare. The desmoplastic ameloblastoma (DA) is characterized by specific clinical, imaging, and histological features. The here presented retrospective analysis investigated the clinicoradiographic features of an overall of 115 DA-cases, having been reported in literature from 1984 to 2008. DA showed a nearly equal male to female ratio (55/59) with a prevalence within the forth and fifth decades. Sixty-two lesions occurred in the mandible and fifty-one lesions in the maxilla. Clinically, a painless swelling with buccal extension was the most common presentation being found in 48 cases. Radiologically, the lesion often presented multilocular (49.3%; 36/73), mixed radiolucent/radiopaque (55.6%; 50/90) and with ill-defined borders (64.0%; 48/75). Whereas enucleation provided a recurrence rate of 21.1%, resection reduced this rate remarkably to 3.1%. The average period until recurrence was 36.9 months. Histologically, scattered epithelial nests and extensively desmoplasia were prominent features of DA. In conclusion, these retrospective results confirm the statement that DA is a variation among ameloblastomas. DA present clinicoradiographic and histologic distinct features, when compared with "conventional ameloblastomas".

    View details for DOI 10.1016/j.oraloncology.2009.01.016

    View details for PubMedID 19631576

    View details for PubMedCentralID PMC6022750

  • Radio-protective effect of Fucoidan on radiation damaged testis tissue in male rats. J Postgrad Wuhan Univ-Medicines Edition Cheng, N., Wu, Y., Zheng, Y., Ren, S., Luo, Q. 2008; 24 (1): 1-15