Professional Education


  • Bachelor of Technology, Unlisted School (2012)
  • Doctor of Philosophy, Centre for Cellular and Molecular Biology (2018)

All Publications


  • ATR activity controls stem cell quiescence via the cyclin F-SCF complex. Proceedings of the National Academy of Sciences of the United States of America Salvi, J. S., Kang, J., Kim, S., Colville, A. J., de Morree, A., Billeskov, T. B., Larsen, M. C., Kanugovi, A., van Velthoven, C. T., Cimprich, K. A., Rando, T. A. 2022; 119 (18): e2115638119

    Abstract

    SignificanceThe replication stress response protein, ATR, is active in quiescent muscle stem cells in response to DNA:RNA hybrids, and this activity maintains quiescence by ensuring degradation of key cell-cycle transition factors by the E3 ubiquitin ligase cyclin F-SCF complex. This is critical for understanding how stem cells regulate a state of prolonged and reversible cell-cycle arrest and how genome integrity is maintained over time. Together, these studies offer a unique picture of a molecular mechanism controlling stem cell quiescence.

    View details for DOI 10.1073/pnas.2115638119

    View details for PubMedID 35476521

  • Alternative polyadenylation of Pax3 controls muscle stem cell fate and muscle function. Science (New York, N.Y.) de Morree, A. n., Klein, J. D., Gan, Q. n., Farup, J. n., Urtasun, A. n., Kanugovi, A. n., Bilen, B. n., van Velthoven, C. T., Quarta, M. n., Rando, T. A. 2019; 366 (6466): 734–38

    Abstract

    Adult stem cells are essential for tissue homeostasis. In skeletal muscle, muscle stem cells (MuSCs) reside in a quiescent state, but little is known about the mechanisms that control homeostatic turnover. Here we show that, in mice, the variation in MuSC activation rate among different muscles (for example, limb versus diaphragm muscles) is determined by the levels of the transcription factor Pax3. We further show that Pax3 levels are controlled by alternative polyadenylation of its transcript, which is regulated by the small nucleolar RNA U1. Isoforms of the Pax3 messenger RNA that differ in their 3' untranslated regions are differentially susceptible to regulation by microRNA miR206, which results in varying levels of the Pax3 protein in vivo. These findings highlight a previously unrecognized mechanism of the homeostatic regulation of stem cell fate by multiple RNA species.

    View details for DOI 10.1126/science.aax1694

    View details for PubMedID 31699935