Adam White is a postdoctoral research fellow in the department of Genetics, jointly supervised by Polly Fordyce and Stephen Quake. He is also the director of the Stanford Microfluidics Foundry. His research focuses on the development of spectrally encoded beads for multiplexed biological assays. Adam received his PhD in Genome Science and Technology from the University of British Columbia for developing integrated microfluidic devices for high-throughput single cell reverse transcription quantitative PCR.
Honors & Awards
Postdoctoral Fellowship, Natural Sciences and Engineering Research Council of Canada (NSERC) (2017 - 2018)
Jump Start Award for Excellence in Research, Stanford University (2016)
Alexander Graham Bell Canada Graduate Scholarship – Doctoral, Natural Sciences and Engineering Research Council of Canada (NSERC) (2012)
Four-Year Fellowship, University of British Columbia (2010)
Paul Geyer Graduate Award in Biomedical Engineering, University of British Columbia (2009)
Junior Research Trainee, Michael Smith Foundation for Health (2008)
Dean of Science Scholarship, University of British Columbia (2006)
Undergraduate Scholar Program Scholarship, University of British Columbia (2001)
Doctor of Philosophy, University of British Columbia (2015)
Postdoc, Stanford University, Genetics (2016)
PhD, University of British Columbia, Genome Science and Technology (2016)
MASc, University of British Columbia, Biomedical Engineering (2010)
BSc, University of British Columbia, Physics (2007)
Polly Fordyce, Postdoctoral Faculty Sponsor
- Outstanding Reviewers for Lab on a Chip in 2017 LAB ON A CHIP 2018; 18 (10): 1398
Highly multiplexed single-cell quantitative PCR
2018; 13 (1): e0191601
We present a microfluidic device for rapid gene expression profiling in single cells using multiplexed quantitative polymerase chain reaction (qPCR). This device integrates all processing steps, including cell isolation and lysis, complementary DNA synthesis, pre-amplification, sample splitting, and measurement in twenty separate qPCR reactions. Each of these steps is performed in parallel on up to 200 single cells per run. Experiments performed on dilutions of purified RNA establish assay linearity over a dynamic range of at least 104, a qPCR precision of 15%, and detection sensitivity down to a single cDNA molecule. We demonstrate the application of our device for rapid profiling of microRNA expression in single cells. Measurements performed on a panel of twenty miRNAs in two types of cells revealed clear cell-to-cell heterogeneity, with evidence of spontaneous differentiation manifested as distinct expression signatures. Highly multiplexed microfluidic RT-qPCR fills a gap in current capabilities for single-cell analysis, providing a rapid and cost-effective approach for profiling panels of marker genes, thereby complementing single-cell genomics methods that are best suited for global analysis and discovery. We expect this approach to enable new studies requiring fast, cost-effective, and precise measurements across hundreds of single cells.
View details for DOI 10.1371/journal.pone.0191601
View details for Web of Science ID 000423514700020
View details for PubMedID 29377915
View details for PubMedCentralID PMC5788347
Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices.
Journal of visualized experiments : JoVE
Microfluidic systems have enabled powerful new approaches to high-throughput biochemical and biological analysis. However, there remains a barrier to entry for non-specialists who would benefit greatly from the ability to develop their own microfluidic devices to address research questions. Particularly lacking has been the open dissemination of protocols related to photolithography, a key step in the development of a replica mold for the manufacture of polydimethylsiloxane (PDMS) devices. While the fabrication of single height silicon masters has been explored extensively in literature, fabrication steps for more complicated photolithography features necessary for many interesting device functionalities (such as feature rounding to make valve structures, multi-height single-mold patterning, or high aspect ratio definition) are often not explicitly outlined. Here, we provide a complete protocol for making multilayer microfluidic devices with valves and complex multi-height geometries, tunable for any application. These fabrication procedures are presented in the context of a microfluidic hydrogel bead synthesizer and demonstrate the production of droplets containing polyethylene glycol (PEG diacrylate) and a photoinitiator that can be polymerized into solid beads. This protocol and accompanying discussion provide a foundation of design principles and fabrication methods that enables development of a wide variety of microfluidic devices. The details included here should allow non-specialists to design and fabricate novel devices, thereby bringing a host of recently developed technologies to their most exciting applications in biological laboratories.
View details for DOI 10.3791/55276
View details for PubMedID 28190039