Andrew D. Huberman

All Publications

• Postsynaptic neuronal activity promotes regeneration of retinal axons. Cell reports Varadarajan, S. G., Wang, F., Dhande, O. S., Le, P., Duan, X., Huberman, A. D. 2023; 42 (5): 112476

  Abstract

  The wiring of visual circuits requires that retinal neurons functionally connect to specific brain targets, a process that involves activity-dependent signaling between retinal axons and their postsynaptic targets. Vision loss in various ophthalmological and neurological diseases is caused by damage to the connections from the eye to the brain. How postsynaptic brain targets influence retinal ganglion cell (RGC) axon regeneration and functional reconnection with the brain targets remains poorly understood. Here, we established a paradigm in which the enhancement of neural activity in the distal optic pathway, where the postsynaptic visual target neurons reside, promotes RGC axon regeneration and target reinnervation and leads to the rescue of optomotor function. Furthermore, selective activation of retinorecipient neuron subsets is sufficient to promote RGC axon regeneration. Our findings reveal a key role for postsynaptic neuronal activity in the repair of neural circuits and highlight the potential to restore damaged sensory inputs via proper brain stimulation.

  View details for DOI 10.1016/j.celrep.2023.112476

  View details for PubMedID 37141093

• Brief structured respiration practices enhance mood and reduce physiological arousal. Cell reports. Medicine Balban, M. Y., Neri, E., Kogon, M. M., Weed, L., Nouriani, B., Jo, B., Holl, G., Zeitzer, J. M., Spiegel, D., Huberman, A. D. 2023: 100895

  Abstract

  Controlled breathwork practices have emerged as potential tools for stress management and well-being. Here, we report a remote, randomized, controlled study (NCT05304000) of three different daily 5-min breathwork exercises compared with an equivalent period of mindfulness meditation over 1 month. The breathing conditions are (1) cyclic sighing, which emphasizes prolonged exhalations; (2) box breathing, which is equal duration of inhalations, breath retentions, and exhalations; and (3) cyclic hyperventilation with retention, with longer inhalations and shorter exhalations. The primary endpoints are improvement in mood and anxiety as well as reduced physiological arousal (respiratory rate, heart rate, and heart rate variability). Using a mixed-effects model, we show that breathwork, especially the exhale-focused cyclic sighing, produces greater improvement in mood (p < 0.05) and reduction in respiratory rate (p < 0.05) compared with mindfulness meditation. Daily 5-min cyclic sighing has promise as an effective stress management exercise.

  View details for DOI 10.1016/j.xcrm.2022.100895

  View details for PubMedID 36630953

• Corrigendum to "Characterization of non-alpha retinal ganglion cell injury responses reveals a possible block to restoring ipRGC function". Experimental neurology Hunyara, J. L., Foshe, S., Varadarajan, S. G., Gribble, K. D., Huberman, A. D., Kolodkin, A. L. 2023; 359: 114256

  View details for DOI 10.1016/j.expneurol.2022.114256

  View details for PubMedID 36457222

• Characterization of non-alpha retinal ganglion cell injury responses reveals a possible block to restoring ipRGC function. Experimental neurology Hunyara, J. L., Foshe, S., Varadarajan, S. G., Gribble, K. D., Huberman, A. D., Kolodkin, A. L. 2022: 114176

  Abstract

  Visual impairment caused by retinal ganglion cell (RGC) axon damage or degeneration affects millions of individuals throughout the world. While some progress has been made in promoting long-distance RGC axon regrowth following injury, it remains unclear whether RGC axons can properly reconnect with their central targets to restore visual function. Additionally, the regenerative capacity of many RGC subtypes remains unknown in part due to a lack of available genetic tools. Here, we use a new mouse line that labels On direction-selective RGCs (oDSGCs) and characterize the survival and regenerative potential of these cells following optic nerve crush (ONC). In parallel, we use a previously characterized mouse line to answer these same questions for M1 intrinsically photosensitive RGCs (ipRGCs). We find that both M1 ipRGCs and oDSGCs are resilient to injury but do not display long-distance axon regrowth following Lin28a overexpression. Unexpectedly, we found that M1 ipRGC, but not oDSGC, intraretinal axons exhibit ectopic branching and are misaligned near the optic disc between one- and three-weeks following injury. Additionally, we observe that numerous ectopic presynaptic specializations associate with misguided ipRGC intraretinal axons. Taken together, these results reveal insights into the injury response of M1 ipRGCs and oDSGCs, providing a foundation for future efforts seeking to restore visual system function following injury.

  View details for DOI 10.1016/j.expneurol.2022.114176

  View details for PubMedID 35870522

• Probing the role of retinorecipient target cells in visual circuit regeneration Varadarajan, S., Dhande, O., Le, P., Huberman, A. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2022

  View details for Web of Science ID 000844437003268

• Central nervous system regeneration. Cell Varadarajan, S. G., Hunyara, J. L., Hamilton, N. R., Kolodkin, A. L., Huberman, A. D. 2022; 185 (1): 77-94

  Abstract

  Neurons of the mammalian central nervous system fail to regenerate. Substantial progress has been made toward identifying the cellular and molecular mechanisms that underlie regenerative failure and how altering those pathways can promote cell survival and/or axon regeneration. Here, we summarize those findings while comparing the regenerative process in the central versus the peripheral nervous system. We also highlight studies that advance our understanding of the mechanisms underlying neural degeneration in response to injury, as many of these mechanisms represent primary targets for restoring functional neural circuits.

  View details for DOI 10.1016/j.cell.2021.10.029

  View details for PubMedID 34995518

• Thalamus: Then and now. The Journal of comparative neurology Guido, W., Huberman, A. 1800

  Abstract

  Introduction to the special issue on thalamus This article is protected by copyright. All rights reserved.

  View details for DOI 10.1002/cne.25288

  View details for PubMedID 34897681

• Divergent outputs of the ventral lateral geniculate nucleus mediate visually evoked defensive behaviors. Cell reports Salay, L. D., Huberman, A. D. 2021; 37 (1): 109792

  Abstract

  Rapid alternations between exploration and defensive reactions require ongoing risk assessment. How visual cues and internal states flexibly modulate the selection of behaviors remains incompletely understood. Here, we show that the ventral lateral geniculate nucleus (vLGN)-a major retinorecipient structure-is a critical node in the network controlling defensive behaviors to visual threats. We find that vLGNGABA neuron activity scales with the intensity of environmental illumination and is modulated by behavioral state. Chemogenetic activation of vLGNGABA neurons reduces freezing, whereas inactivation dramatically extends the duration of freezing to visual threats. Perturbations of vLGN activity disrupt exploration in brightly illuminated environments. We describe both a vLGNnucleus reuniens (Re) circuit and a vLGNsuperior colliculus (SC) circuit, which exert opposite influences on defensive responses. These findings reveal roles for genetic- and projection-defined vLGN subpopulations in modulating the expression of behavioral threat responses according to internal state.

  View details for DOI 10.1016/j.celrep.2021.109792

  View details for PubMedID 34610302

• Human Responses to Visually Evoked Threat. Current biology : CB Yilmaz Balban, M. n., Cafaro, E. n., Saue-Fletcher, L. n., Washington, M. J., Bijanzadeh, M. n., Lee, A. M., Chang, E. F., Huberman, A. D. 2020

  Abstract

  Vision is the primary sense humans use to evaluate and respond to threats. Understanding the biological underpinnings of the human threat response has been hindered by lack of realistic in-lab threat paradigms. We established an immersive virtual reality (VR) platform to simultaneously measure behavior, physiological state, and neural activity from the human brain using chronically implanted electrodes. Subjects with high anxiety showed increased visual scanning in response to threats as compared to healthy controls. In both healthy and anxious subjects, the amount of scanning behavior correlated with the magnitude of physiological arousal, suggesting that visual scanning behavior is directly linked to internal state. Intracranial electroencephalography (iEEG) recordings from three subjects suggested that high-frequency gamma activity in the insula positively correlates with physiological arousal induced by visual threats and that low-frequency theta activity in the orbitofrontal cortex (OFC) negatively correlates with physiological arousal induced by visual threats. These findings reveal a key role of eye movements and suggest that distinct insula and OFC activation dynamics may be important for detecting and adjusting human stress in response to visually perceived threats.

  View details for DOI 10.1016/j.cub.2020.11.035

  View details for PubMedID 33242389

• Neuroscience: A Chromatic Retinal Circuit Encodes Sunrise and Sunset for the Brain. Current biology : CB Rivera, A. M., Huberman, A. D. 2020; 30 (7): R316–R318

  Abstract

  A new study reveals the retinal circuit for encoding the types of light prominent at sunrise and sunset. The output of that circuit is conveyed to the brain's master circadian clock. Subconscious processing of sky color changes may therefore be the key stimulus for conveying morning and evening information to the circadian timing system in the brain.

  View details for DOI 10.1016/j.cub.2020.02.090

  View details for PubMedID 32259506

• Neurotoxic Reactive Astrocytes Drive Neuronal Death after Retinal Injury. Cell reports Guttenplan, K. A., Stafford, B. K., El-Danaf, R. N., Adler, D. I., Münch, A. E., Weigel, M. K., Huberman, A. D., Liddelow, S. A. 2020; 31 (12): 107776

  Abstract

  Glaucoma is a neurodegenerative disease that features the death of retinal ganglion cells (RGCs) in the retina, often as a result of prolonged increases in intraocular pressure. We show that preventing the formation of neuroinflammatory reactive astrocytes prevents the death of RGCs normally seen in a mouse model of glaucoma. Furthermore, we show that these spared RGCs are electrophysiologically functional and thus still have potential value for the function and regeneration of the retina. Finally, we demonstrate that the death of RGCs depends on a combination of both an injury to the neurons and the presence of reactive astrocytes, suggesting a model that may explain why reactive astrocytes are toxic only in some circumstances. Altogether, these findings highlight reactive astrocytes as drivers of RGC death in a chronic neurodegenerative disease of the eye.

  View details for DOI 10.1016/j.celrep.2020.107776

  View details for PubMedID 32579912

• Sight restored by turning back the epigenetic clock. Nature Huberman, A. D. 2020; 588 (7836): 34–36

  View details for DOI 10.1038/d41586-020-03119-1

  View details for PubMedID 33268872

• Molecular Fingerprinting of On-Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits JOURNAL OF NEUROSCIENCE Dhande, O. S., Stafford, B. K., Franke, K., El-Danaf, R., Percival, K. A., Phan, A. H., Li, P., Hansen, B. J., Nguyen, P. L., Berens, P., Taylor, W., Callaway, E., Euler, T., Huberman, A. D. 2019; 39 (1): 78-95

  View details for DOI 10.1523/JNEUROSCI.1784-18.2018

  View details for Web of Science ID 000454689500008

• Fear: It's All in Your Line of Sight. Current biology : CB Yilmaz, M. n., Huberman, A. D. 2019; 29 (23): R1232–R1234

  Abstract

  The brain circuits that create our sense of fear rely on ancient 'hard-wired' components of the limbic system, but also use sensory processing to determine what we become afraid of. A new study shows that, when viewing of simple oriented line stimuli is coupled with aversive experiences, neurons in primary visual cortex rapidly alter their responses in a manner that indicates the line stimuli become a source of fear.

  View details for DOI 10.1016/j.cub.2019.10.008

  View details for PubMedID 31794754

• Sub-topographic maps for regionally enhanced analysis of visual space in the mouse retina JOURNAL OF COMPARATIVE NEUROLOGY El-Danaf, R. N., Huberman, A. D. 2019; 527 (1): 259-269

  View details for DOI 10.1002/cne.24457

  View details for Web of Science ID 000457345800018

• Introduction to retinal special issue I. The Journal of comparative neurology Huberman, A. D., Berson, D. M. 2019; 527 (1): 7–8

  View details for DOI 10.1002/cne.24599

  View details for PubMedID 30597554

• Dedication of Retinal Special Issue to: Harvey J. Karten, MD JOURNAL OF COMPARATIVE NEUROLOGY Huberman, A. D. 2019; 527 (1): 9–10

  View details for DOI 10.1002/cne.24601

  View details for Web of Science ID 000457345800003

  View details for PubMedID 30597552

• An Unbiased View of Neural Networks: More than Meets the Eye NEURON Jung, H., Huberman, A. D. 2018; 100 (5): 1019–21

  View details for DOI 10.1016/j.neuron.2018.11.038

  View details for Web of Science ID 000452295400006

• An Unbiased View of Neural Networks: More than Meets the Eye. Neuron Jung, H., Huberman, A. D. 2018; 100 (5): 1019-1021

  Abstract

  In this issue of Neuron, Macé et al. (2018) use whole-brain functional ultrasound imaging in mice to unveil the circuits involved reflexive eye movements. They separated the sensory and motor networks and discovered that certain eye movements robustly suppress the amygdala.

  View details for DOI 10.1016/j.neuron.2018.11.038

  View details for PubMedID 30521775

• Assembly and repair of eye-to-brain connections CURRENT OPINION IN NEUROBIOLOGY Varadarajan, S. G., Huberman, A. D. 2018; 53: 198-209

  View details for DOI 10.1016/j.conb.2018.10.001

  View details for Web of Science ID 000451938500027

• Synaptic Convergence Patterns onto Retinal Ganglion Cells Are Preserved despite Topographic Variation in Pre- and Postsynaptic Territories. Cell reports Yu, W., El-Danaf, R. N., Okawa, H., Pacholec, J. M., Matti, U., Schwarz, K., Odermatt, B., Dunn, F. A., Lagnado, L., Schmitz, F., Huberman, A. D., Wong, R. O. 2018; 25 (8): 2017

  Abstract

  Sensory processing can be tuned by a neuron's integration area, the types of inputs, and the proportion and number of connections with those inputs. Integration areas often vary topographically to sample space differentially across regions. Here, we highlight two visual circuits in which topographic changes in the postsynaptic retinal ganglion cell (RGC) dendritic territories and their presynaptic bipolar cell (BC) axonal territories are either matched or unmatched. Despite this difference, in both circuits, the proportion of inputs from each BC type, i.e., synaptic convergence between specific BCs and RGCs, remained constant across varying dendritic territory sizes. Furthermore, synapse density between BCs and RGCs was invariant across topography. Our results demonstrate a wiring design, likely engaging homotypic axonal tiling of BCs, that ensures consistency in synaptic convergence between specific BC types onto their target RGCs while enabling independent regulation of pre- and postsynaptic territory sizes and synapse number between cell pairs.

  View details for PubMedID 30463000

• Synaptic Convergence Patterns onto Retinal Ganglion Cells Are Preserved despite Topographic Variation in Pre- and Postsynaptic Territories CELL REPORTS Yu, W., El-Danaf, R. N., Okawa, H., Pacholec, J. M., Matti, U., Schwarz, K., Odermatt, B., Dunn, F. A., Lagnado, L., Schmitz, F., Huberman, A. D., Wong, R. L. 2018; 25 (8): 2017-+

  View details for DOI 10.1016/j.celrep.2018.10.089

  View details for Web of Science ID 000450794200003

• Molecular fingerprinting of On-Off direction selective retinal ganglion cells across species and relevance to primate visual circuits. The Journal of neuroscience : the official journal of the Society for Neuroscience Dhande, O. S., Stafford, B. K., Franke, K., El-Danaf, R., Percival, K. A., Phan, A. H., Li, P., Hansen, B. J., Nguyen, P. L., Berens, P., Taylor, W. R., Callaway, E., Euler, T., Huberman, A. D. 2018

  Abstract

  The ability to detect moving objects is an ethologically salient function. Direction selective neurons have been identified in the retina, thalamus, and cortex of many species, but their homology has remained opaque. For instance, it is unknown whether direction-selective retinal ganglion cells (DSGCs) exist in primates, and if so, whether they are the equivalent to mouse and rabbit DSGCs. Here, we used a molecular/circuit approach in both sexes to address these issues. In mice, we identify the transcription factor Satb2 (Special AT-rich sequence-binding protein 2) as a selective marker for three RGC types: On-Off DSGCs encoding motion in either the anterior or posterior direction, a newly identified type of Off-DSGC and an Off-sustained RGC type. In rabbits, we find that expression of Satb2 is conserved in On-Off DSGCs; however, has evolved to include On-Off DSGCs encoding upward and downward motion, in addition to anterior and posterior motion. Next, we show that macaque RGCs express Satb2 most likely in a single type. We used rabies-virus based circuit mapping tools to reveal the identity of macaque Satb2-RGCs and discovered their dendritic arbors are relatively large and monostratified. Together, these data indicate Satb2-expressing On-Off DSGCs are likely not present in the primate retina. Moreover, if DSGCs are present in the primate retina, it is unlikely that they express Satb2.SIGNIFICANCE STATEMENTThe ability to detect object-motion is a fundamental feature of almost all visual systems. Here we identify a novel marker for retinal ganglion cells encoding directional motion that is evolutionarily conserved in mice and rabbits, but not in primates. We show that in macaque monkeys the retinal ganglion cells that express this marker comprise a single type and are morphologically distinct from mouse and rabbit direction-selective retinal ganglion cells. Our findings indicate that On-Off direction-selective retinal neurons may have evolutionarily diverged in primates and more generally provide novel insight into the identity and organization of primate parallel visual pathways.

  View details for PubMedID 30377226

• Assembly and repair of eye-to-brain connections. Current opinion in neurobiology Varadarajan, S. G., Huberman, A. D. 2018; 53: 198–209

  Abstract

  Vision is the sense humans rely on most to navigate the world and survive. A tremendous amount of research has focused on understanding the neural circuits for vision and the developmental mechanisms that establish them. The eye-to-brain, or 'retinofugal' pathway remains a particularly important model in these contexts because it is essential for sight, its overt anatomical features relate to distinct functional attributes and those features develop in a tractable sequence. Much progress has been made in understanding the growth of retinal axons out of the eye, their selection of targets in the brain, the development of laminar and cell type-specific connectivity within those targets, and also dendritic connectivity within the retina itself. Moreover, because the retinofugal pathway is prone to degeneration in many common blinding diseases, understanding the cellular and molecular mechanisms that establish connectivity early in life stands to provide valuable insights into approaches that re-wire this pathway after damage or loss. Here we review recent progress in understanding the development of retinofugal pathways and how this information is important for improving visual circuit regeneration.

  View details for PubMedID 30339988

• Sub-topographic maps for regionally enhanced analysis of visual space in the mouse retina. The Journal of comparative neurology El-Danaf, R. N., Huberman, A. D. 2018

  Abstract

  In many species, neurons are unevenly distributed across the retina, leading to nonuniform analysis of specific visual features at certain locations in visual space. In recent years, the mouse has emerged as a premiere model for probing visual system function, development and disease. Thus, achieving a detailed understanding of mouse visual circuit architecture is of paramount importance. The general belief is that mice possess a relatively even topographic distribution of retinal ganglion cells (RGCs)- the output neurons of the eye. However, mouse RGCs include 30 subtypes; each responds best to a specific feature in the visual scene and conveys that information to central targets. Given the crucial role of RGCs and the prominence of the mouse as a model, we asked how different RGC subtypes are distributed across the retina. We targeted and filled individual fluorescently tagged RGC subtypes from across the retinal surface and evaluated the dendritic arbor extent and soma size of each cell according to its specific retinotopic position. Three prominent RGC subtypes: On-Off direction selective RGCs, object-motion-sensitive RGCs, and a specialized subclass of non-image-forming RGCs each had marked topographic variations in their dendritic arbor sizes. Moreover, the pattern of variation was distinct for each RGC subtype. Thus, there is increasing evidence that the mouse retina encodes visual space in a region-specific manner. As a consequence, some visual features are sampled far more densely at certain retinal locations than others. These findings have implications for central visual processing, perception and behavior in this prominent model species. This article is protected by copyright. All rights reserved.

  View details for PubMedID 29675855

• Ben Barres (1954-2017) OBITUARY NATURE Huberman, A. D. 2018; 553 (7688): 282

  View details for Web of Science ID 000423475100035

  View details for PubMedID 29345654

• A midline thalamic circuit determines reactions to visual threat. Nature Salay, L. D., Ishiko, N. n., Huberman, A. D. 2018

  Abstract

  How our internal state is merged with our visual perception of an impending threat to drive an adaptive behavioural response is not known. Mice respond to visual threats by either freezing or seeking shelter. Here we show that nuclei of the ventral midline thalamus (vMT), the xiphoid nucleus (Xi) and nucleus reuniens (Re), represent crucial hubs in the network controlling behavioural responses to visual threats. The Xi projects to the basolateral amygdala to promote saliency-reducing responses to threats, such as freezing, whereas the Re projects to the medial prefrontal cortex (Re→mPFC) to promote saliency-enhancing, even confrontational responses to threats, such as tail rattling. Activation of the Re→mPFC pathway also increases autonomic arousal in a manner that is rewarding. The vMT is therefore important for biasing how internal states are translated into opposing categories of behavioural responses to perceived threats. These findings may have implications for understanding disorders of arousal and adaptive decision-making, such as phobias, post-traumatic stress and addictions.

  View details for PubMedID 29720647

• Uniformity from Diversity: Vast-Range Light Sensing in a Single Neuron Type CELL Varadarajan, S. G., Huberman, A. D. 2017; 171 (4): 738–40

  Abstract

  The brightness of our visual environment varies tremendously from day to night. In this issue of Cell, Milner and Do describe how the population of retinal neurons responsible for entrainment of the brain's circadian clock cooperate to encode irradiance across a wide range of ambient-light intensities.

  View details for PubMedID 29100070

• Developmental mechanisms for establishing functional non-image-forming visual circuits Dhande, O. S., Phan, A. H., Seabrook, T. A., Nguyen, P. L., Wang, J. T., Huberman, A. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2017

  View details for Web of Science ID 000432170304131

• Distinct retinal ganglion cell subtypes exhibit diverse topographic characteristics across the mouse retina El-Danaf, R., Huberman, A. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2017

  View details for Web of Science ID 000432170306309

• Signal Integration in Thalamus: Labeled Lines Go Cross-Eyed and Blurry. Neuron Stafford, B. K., Huberman, A. D. 2017; 93 (4): 717-720

  Abstract

  The brain uses sensory information from the periphery to create percepts. In this issue of Neuron, Rompani et al. (2017) show that visual signals are combined in unexpected ways that vastly expand the possible representations of the outside world.

  View details for DOI 10.1016/j.neuron.2017.02.020

  View details for PubMedID 28231456

• Regenerating optic pathways from the eye to the brain. Science (New York, N.Y.) Laha, B. n., Stafford, B. K., Huberman, A. D. 2017; 356 (6342): 1031–34

  Abstract

  Humans are highly visual. Retinal ganglion cells (RGCs), the neurons that connect the eyes to the brain, fail to regenerate after damage, eventually leading to blindness. Here, we review research on regeneration and repair of the optic system. Intrinsic developmental growth programs can be reactivated in RGCs, neural activity can enhance RGC regeneration, and functional reformation of eye-to-brain connections is possible, even in the adult brain. Transplantation and gene therapy may serve to replace or resurrect dead or injured retinal neurons. Retinal prosthetics that can restore vision in animal models may too have practical power in the clinical setting. Functional restoration of sight in certain forms of blindness is likely to occur in human patients in the near future.

  View details for PubMedID 28596336

• Architecture, Function, and Assembly of the Mouse Visual System. Annual review of neuroscience Seabrook, T. A., Burbridge, T. J., Crair, M. C., Huberman, A. D. 2017; 40: 499–538

  Abstract

  Vision is the sense humans rely on most to navigate the world, make decisions, and perform complex tasks. Understanding how humans see thus represents one of the most fundamental and important goals of neuroscience. The use of the mouse as a model for parsing how vision works at a fundamental level started approximately a decade ago, ushered in by the mouse's convenient size, relatively low cost, and, above all, amenability to genetic perturbations. In the course of that effort, a large cadre of new and powerful tools for in vivo labeling, monitoring, and manipulation of neurons were applied to this species. As a consequence, a significant body of work now exists on the architecture, function, and development of mouse central visual pathways. Excitingly, much of that work includes causal testing of the role of specific cell types and circuits in visual perception and behavior-something rare to find in studies of the visual system of other species. Indeed, one could argue that more information is now available about the mouse visual system than any other sensory system, in any species, including humans. As such, the mouse visual system has become a platform for multilevel analysis of the mammalian central nervous system generally. Here we review the mouse visual system structure, function, and development literature and comment on the similarities and differences between the visual system of this and other model species. We also make it a point to highlight the aspects of mouse visual circuitry that remain opaque and that are in need of additional experimentation to enrich our understanding of how vision works on a broad scale.

  View details for PubMedID 28772103

• Strict Independence of Parallel and Poly-synaptic Axon-Target Matching during Visual Reflex Circuit Assembly. Cell reports Seabrook, T. A., Dhande, O. S., Ishiko, N. n., Wooley, V. P., Nguyen, P. L., Huberman, A. D. 2017; 21 (11): 3049–64

  Abstract

  The use of sensory information to drive specific behaviors relies on circuits spanning long distances that wire up through a range of axon-target recognition events. Mechanisms assembling poly-synaptic circuits and the extent to which parallel pathways can "cross-wire" to compensate for loss of one another remain unclear and are crucial to our understanding of brain development and models of regeneration. In the visual system, specific retinal ganglion cells (RGCs) project to designated midbrain targets connected to downstream circuits driving visuomotor reflexes. Here, we deleted RGCs connecting to pupillary light reflex (PLR) midbrain targets and discovered that axon-target matching is tightly regulated. RGC axons of the eye-reflex pathway avoided vacated PLR targets. Moreover, downstream PLR circuitry is maintained; hindbrain and peripheral components retained their proper connectivity and function. These findings point to a model in which poly-synaptic circuit development reflects independent, highly stringent wiring of each parallel pathway and downstream station.

  View details for PubMedID 29241535

• Cortico-fugal output from visual cortex promotes plasticity of innate motor behaviour. Nature Liu, B., Huberman, A. D., Scanziani, M. 2016; 538 (7625): 383-387

  Abstract

  The mammalian visual cortex massively innervates the brainstem, a phylogenetically older structure, via cortico-fugal axonal projections. Many cortico-fugal projections target brainstem nuclei that mediate innate motor behaviours, but the function of these projections remains poorly understood. A prime example of such behaviours is the optokinetic reflex (OKR), an innate eye movement mediated by the brainstem accessory optic system, that stabilizes images on the retina as the animal moves through the environment and is thus crucial for vision. The OKR is plastic, allowing the amplitude of this reflex to be adaptively adjusted relative to other oculomotor reflexes and thereby ensuring image stability throughout life. Although the plasticity of the OKR is thought to involve subcortical structures such as the cerebellum and vestibular nuclei, cortical lesions have suggested that the visual cortex might also be involved. Here we show that projections from the mouse visual cortex to the accessory optic system promote the adaptive plasticity of the OKR. OKR potentiation, a compensatory plastic increase in the amplitude of the OKR in response to vestibular impairment, is diminished by silencing visual cortex. Furthermore, targeted ablation of a sparse population of cortico-fugal neurons that specifically project to the accessory optic system severely impairs OKR potentiation. Finally, OKR potentiation results from an enhanced drive exerted by the visual cortex onto the accessory optic system. Thus, cortico-fugal projections to the brainstem enable the visual cortex, an area that has been principally studied for its sensory processing function, to plastically adapt the execution of innate motor behaviours.

  View details for DOI 10.1038/nature19818

  View details for PubMedID 27732573

• Neural activity promotes long-distance, target-specific regeneration of adult retinal axons. Nature neuroscience Lim, J. A., Stafford, B. K., Nguyen, P. L., Lien, B. V., Wang, C., Zukor, K., He, Z., Huberman, A. D. 2016; 19 (8): 1073-1084

  Abstract

  Axons in the mammalian CNS fail to regenerate after injury. Here we show that if the activity of mouse retinal ganglion cells (RGCs) is increased by visual stimulation or using chemogenetics, their axons regenerate. We also show that if enhancement of neural activity is combined with elevation of the cell-growth-promoting pathway involving mammalian target of rapamycin (mTOR), RGC axons regenerate long distances and re-innervate the brain. Analysis of genetically labeled RGCs revealed that this regrowth can be target specific: RGC axons navigated back to their correct visual targets and avoided targets incorrect for their function. Moreover, these regenerated connections were successful in partially rescuing a subset of visual behaviors. Our findings indicate that combining neural activity with activation of mTOR can serve as powerful tool for enhancing axon regeneration, and they highlight the remarkable capacity of CNS neurons to re-establish accurate circuit connections in adulthood.

  View details for DOI 10.1038/nn.4340

  View details for PubMedID 27399843

• Report on the National Eye Institute Audacious Goals Initiative: Regenerating the Optic Nerve. Investigative ophthalmology & visual science Goldberg, J. L., Guido, W., For The Agi Workshop Participants 2016; 57 (3): 1271-1275

  Abstract

  The National Eye Institute (NEI) hosted a workshop on November 19, 2014, as part of the Audacious Goals Initiative (AGI), an NEI-led effort to rapidly expand therapies for eye diseases through coordinated research funding. The central audacious goal aims to demonstrate by 2025 the restoration of usable vision in humans through the regeneration of neurons and neural connections in the eye and visual system. This workshop focused on identifying promising strategies for optic nerve regeneration. Its principal objective was to solicit input on future AGI-related funding announcements, and specifically to ask, where are we now in our scientific progress, and what progress should we reach for in the coming years? A full report was generated as a white paper posted on the NEI Web site; this report summarizes the discussion and outcomes from the meeting and serves as guidance for future funding of research that focuses on optic nerve regeneration.

  View details for DOI 10.1167/iovs.15-18500

  View details for PubMedID 26990163

• Life goes by: a visual circuit signals perceptual-motor mismatch NATURE NEUROSCIENCE Ishiko, N., Huberman, A. D. 2016; 19 (2): 177-179

  View details for DOI 10.1038/nn.4233

  View details for Web of Science ID 000369172600003

  View details for PubMedID 26814584

• BLINDNESS Assassins of eyesight NATURE Huberman, A. D., El-Danaf, R. N. 2015; 527 (7579): 456-457

  View details for Web of Science ID 000365352500034

  View details for PubMedID 26607542

• Cell type-specific manipulation with GFP-dependent Cre recombinase NATURE NEUROSCIENCE Tang, J. C., Rudolph, S., Dhande, O. S., Abraira, V. E., Choi, S., Lapan, S. W., Drew, I. R., Drokhlyansky, E., Huberman, A. D., Regehr, W. G., Cepko, C. L. 2015; 18 (9): 1334-?

  Abstract

  There are many transgenic GFP reporter lines that allow the visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation. We developed a method that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split component system that uses GFP and its derivatives to directly induce Cre/loxP recombination. Using plasmid electroporation and AAV viral vectors, we delivered CRE-DOG to multiple GFP mouse lines, which led to effective recombination selectively in GFP-labeled cells. Furthermore, CRE-DOG enabled optogenetic control of these neurons. Beyond providing a new set of tools for manipulation of gene expression selectively in GFP(+) cells, we found that GFP can be used to reconstitute the activity of a protein not known to have a modular structure, suggesting that this strategy might be applicable to a wide range of proteins.

  View details for DOI 10.1038/nn.4081

  View details for Web of Science ID 000360292600024

  View details for PubMedID 26258682

• When Visual Circuits Collide: Motion Processing in the Brain. Cell Salay, L. D., Huberman, A. D. 2015; 162 (2): 241-243

  Abstract

  How is sensory information transformed by each station of a synaptic circuit as it flows progressively deeper into the brain? In this issue of Cell, Mauss et al. describe a set of connections in the fly brain that combines opposing directional signals, and they hypothesize that this motif limits global motion noise as the fly moves through space.

  View details for DOI 10.1016/j.cell.2015.06.051

  View details for PubMedID 26186184

• Cortical Cliques: A Few Plastic Neurons Get All the Action NEURON Seabrook, T. A., Huberman, A. D. 2015; 86 (5): 1113-1116

  Abstract

  Adjustments in neural activity can drive cortical plasticity, but the underlying circuit components remain unclear. In this issue of Neuron, Barnes et al. (2015) show that visual deprivation-induced homeostatic plasticity invokes specific changes among select categories of V1 neurons.

  View details for DOI 10.1016/j.neuron.2015.05.039

  View details for Web of Science ID 000355666400002

  View details for PubMedID 26050030

• Contactin-4 Mediates Axon-Target Specificity and Functional Development of the Accessory Optic System NEURON Osterhout, J. A., Stafford, B. K., Nguyen, P. L., Yoshihara, Y., Huberman, A. D. 2015; 86 (4): 985-999

  Abstract

  The mammalian eye-to-brain pathway includes more than 20 parallel circuits, each consisting of precise long-range connections between specific sets of retinal ganglion cells (RGCs) and target structures in the brain. The mechanisms that drive assembly of these parallel connections and the functional implications of their specificity remain unresolved. Here we show that in the absence of contactin 4 (CNTN4) or one of its binding partners, amyloid precursor protein (APP), a subset of direction-selective RGCs fail to target the nucleus of the optic tract (NOT)--the accessory optic system (AOS) target controlling horizontal image stabilization. Conversely, ectopic expression of CNTN4 biases RGCs to arborize in the NOT, and that process also requires APP. Our data reveal critical and novel roles for CNTN4/APP in promoting target-specific axon arborization, and they highlight the importance of this process for functional development of a behaviorally relevant parallel visual pathway.

  View details for DOI 10.1016/j.neuron.2015.04.005

  View details for Web of Science ID 000354878400013

  View details for PubMedID 25959733

  View details for PubMedCentralID PMC4706364

• Functional Assembly of Accessory Optic System Circuitry Critical for Compensatory Eye Movements. Neuron Sun, L. O., Brady, C. M., Cahill, H., Al-Khindi, T., Sakuta, H., Dhande, O. S., Noda, M., Huberman, A. D., Nathans, J., Kolodkin, A. L. 2015

  Abstract

  Accurate motion detection requires neural circuitry that compensates for global visual field motion. Select subtypes of retinal ganglion cells perceive image motion and connect to the accessory optic system (AOS) in the brain, which generates compensatory eye movements that stabilize images during slow visual field motion. Here, we show that the murine transmembrane semaphorin 6A (Sema6A) is expressed in a subset of On direction-selective ganglion cells (On DSGCs) and is required for retinorecipient axonal targeting to the medial terminal nucleus (MTN) of the AOS. Plexin A2 and A4, two Sema6A binding partners, are expressed in MTN cells, attract Sema6A(+) On DSGC axons, and mediate MTN targeting of Sema6A(+) RGC projections. Furthermore, Sema6A/Plexin-A2/A4 signaling is required for the functional output of the AOS. These data reveal molecular mechanisms underlying the assembly of AOS circuits critical for moving image perception.

  View details for DOI 10.1016/j.neuron.2015.03.064

  View details for PubMedID 25959730

• Characteristic Patterns of Dendritic Remodeling in Early-Stage Glaucoma: Evidence from Genetically Identified Retinal Ganglion Cell Types JOURNAL OF NEUROSCIENCE El-Danaf, R. N., Huberman, A. D. 2015; 35 (6): 2329-2343

  Abstract

  Retinal ganglion cell (RGC) loss is a hallmark of glaucoma and the second leading cause of blindness worldwide. The type and timing of cellular changes leading to RGC loss in glaucoma remain incompletely understood, including whether specific RGC subtypes are preferentially impacted at early stages of this disease. Here we applied the microbead occlusion model of glaucoma to different transgenic mouse lines, each expressing green fluorescent protein in 1-2 specific RGC subtypes. Targeted filling, reconstruction, and subsequent comparison of the genetically identified RGCs in control and bead-injected eyes revealed that some subtypes undergo significant dendritic rearrangements as early as 7 d following induction of elevated intraocular pressure (IOP). By comparing specific On-type, On-Off-type and Off-type RGCs, we found that RGCs that target the majority of their dendritic arbors to the scleral half or "Off" sublamina of the inner plexiform layer (IPL) undergo the greatest changes, whereas RGCs with the majority of their dendrites in the On sublamina did not alter their structure at this time point. Moreover, M1 intrinsically photosensitive RGCs, which functionally are On RGCs but structurally stratify their dendrites in the Off sublamina of the IPL, also underwent significant changes in dendritic structure 1 week after elevated IOP. Thus, our findings reveal that certain RGC subtypes manifest significant changes in dendritic structure after very brief exposure to elevated IOP. The observation that RGCs stratifying most of their dendrites in the Off sublamina are first to alter their structure may inform the development of new strategies to detect, monitor, and treat glaucoma in humans.

  View details for DOI 10.1523/JNEUROSCI.1419-14.2015

  View details for Web of Science ID 000349686500003

  View details for PubMedID 25673829

• So many pieces, one puzzle: cell type specification and visual circuitry in flies and mice GENES & DEVELOPMENT Wernet, M. F., Huberman, A. D., Desplan, C. 2014; 28 (23): 2565-2584

  Abstract

  The visual system is a powerful model for probing the development, connectivity, and function of neural circuits. Two genetically tractable species, mice and flies, are together providing a great deal of understanding of these processes. Current efforts focus on integrating knowledge gained from three cross-fostering fields of research: (1) understanding how the fates of different cell types are specified during development, (2) revealing the synaptic connections between identified cell types ("connectomics") by high-resolution three-dimensional circuit anatomy, and (3) causal testing of how identified circuit elements contribute to visual perception and behavior. Here we discuss representative examples from fly and mouse models to illustrate the ongoing success of this tripartite strategy, focusing on the ways it is enhancing our understanding of visual processing and other sensory systems.

  View details for DOI 10.1101/gad.248245.114

  View details for Web of Science ID 000345812000001

  View details for PubMedID 25452270

  View details for PubMedCentralID PMC4248288

• Birthdate and Outgrowth Timing Predict Cellular Mechanisms of Axon Target Matching in the Developing Visual Pathway CELL REPORTS Osterhout, J. A., El-Danaf, R. N., Nguyen, P. L., Huberman, A. D. 2014; 8 (4): 1006-1017

  Abstract

  How axons select their appropriate targets in the brain remains poorly understood. Here, we explore the cellular mechanisms of axon target matching in the developing visual system by comparing four transgenic mouse lines, each with a different population of genetically labeled retinal ganglion cells (RGCs) that connect to unique combinations of brain targets. We find that the time when an RGC axon arrives in the brain is correlated with its target selection strategy. Early-born, early-arriving RGC axons initially innervate multiple targets. Subsequently, most of those connections are removed. By contrast, later-born, later-arriving RGC axons are highly accurate in their initial target choices. These data reveal the diversity of cellular mechanisms that mammalian CNS axons use to pick their targets and highlight the key role of birthdate and outgrowth timing in influencing this precision. Timing-based mechanisms may underlie the assembly of the other sensory pathways and complex neural circuitry in the brain.

  View details for DOI 10.1016/j.celrep.2014.06.063

  View details for Web of Science ID 000341573500011

  View details for PubMedID 25088424

  View details for PubMedCentralID PMC4143387

• A dedicated circuit links direction-selective retinal ganglion cells to the primary visual cortex NATURE Cruz-Martin, A., El-Danaf, R. N., Osakada, F., Sriram, B., Dhande, O. S., Nguyen, P. L., Callaway, E. M., Ghosh, A., Huberman, A. D. 2014; 507 (7492): 358-?

  Abstract

  How specific features in the environment are represented within the brain is an important unanswered question in neuroscience. A subset of retinal neurons, called direction-selective ganglion cells (DSGCs), are specialized for detecting motion along specific axes of the visual field. Despite extensive study of the retinal circuitry that endows DSGCs with their unique tuning properties, their downstream circuitry in the brain and thus their contribution to visual processing has remained unclear. In mice, several different types of DSGCs connect to the dorsal lateral geniculate nucleus (dLGN), the visual thalamic structure that harbours cortical relay neurons. Whether direction-selective information computed at the level of the retina is routed to cortical circuits and integrated with other visual channels, however, is unknown. Here we show that there is a di-synaptic circuit linking DSGCs with the superficial layers of the primary visual cortex (V1) by using viral trans-synaptic circuit mapping and functional imaging of visually driven calcium signals in thalamocortical axons. This circuit pools information from several types of DSGCs, converges in a specialized subdivision of the dLGN, and delivers direction-tuned and orientation-tuned signals to superficial V1. Notably, this circuit is anatomically segregated from the retino-geniculo-cortical pathway carrying non-direction-tuned visual information to deeper layers of V1, such as layer 4. Thus, the mouse harbours several functionally specialized, parallel retino-geniculo-cortical pathways, one of which originates with retinal DSGCs and delivers direction- and orientation-tuned information specifically to the superficial layers of the primary visual cortex. These data provide evidence that direction and orientation selectivity of some V1 neurons may be influenced by the activation of DSGCs.

  View details for DOI 10.1038/nature12989

  View details for Web of Science ID 000333029000034

  View details for PubMedID 24572358

  View details for PubMedCentralID PMC4143386

• Visual Circuits: Mouse Retina No Longer a Level Playing Field CURRENT BIOLOGY Dhande, O. S., Huberman, A. D. 2014; 24 (4): R155-R156

  Abstract

  Unlike humans, monkeys, or carnivores, mice are thought to lack a retinal subregion devoted to high-resolution vision; systematic analysis has now shown that mice encode visual space non-uniformly, increasing their spatial sampling of the binocular visual field.

  View details for DOI 10.1016/j.cub.2013.12.045

  View details for Web of Science ID 000331718900010

  View details for PubMedID 24556437

• Retinal ganglion cell maps in the brain: implications for visual processing CURRENT OPINION IN NEUROBIOLOGY Dhande, O. S., Huberman, A. D. 2014; 24: 133-142

  Abstract

  Everything the brain knows about the content of the visual world is built from the spiking activity of retinal ganglion cells (RGCs). As the output neurons of the eye, RGCs include ∼20 different subtypes, each responding best to a specific feature in the visual scene. Here we discuss recent advances in identifying where different RGC subtypes route visual information in the brain, including which targets they connect to and how their organization within those targets influences visual processing. We also highlight examples where causal links have been established between specific RGC subtypes, their maps of central connections and defined aspects of light-mediated behavior and we suggest the use of techniques that stand to extend these sorts of analyses to circuits underlying visual perception.

  View details for DOI 10.1016/j.conb.2013.08.006

  View details for Web of Science ID 000331509500020

  View details for PubMedID 24492089

  View details for PubMedCentralID PMC4086677

• Genetic Dissection of Retinal Inputs to Brainstem Nuclei Controlling Image Stabilization JOURNAL OF NEUROSCIENCE Dhande, O. S., Estevez, M. E., Quattrochi, L. E., El-Danaf, R. N., Nguyen, P. L., Berson, D. M., Huberman, A. D. 2013; 33 (45): 17797-17813

  Abstract

  When the head rotates, the image of the visual world slips across the retina. A dedicated set of retinal ganglion cells (RGCs) and brainstem visual nuclei termed the "accessory optic system" (AOS) generate slip-compensating eye movements that stabilize visual images on the retina and improve visual performance. Which types of RGCs project to each of the various AOS nuclei remain unresolved. Here we report a new transgenic mouse line, Hoxd10-GFP, in which the RGCs projecting to all the AOS nuclei are fluorescently labeled. Electrophysiological recordings of Hoxd10-GFP RGCs revealed that they include all three subtypes of On direction-selective RGCs (On-DSGCs), responding to upward, downward, or forward motion. Hoxd10-GFP RGCs also include one subtype of On-Off DSGCs tuned for forward motion. Retrograde circuit mapping with modified rabies viruses revealed that the On-DSGCs project to the brainstem centers involved in both horizontal and vertical retinal slip compensation. In contrast, the On-Off DSGCs labeled in Hoxd10-GFP mice projected to AOS nuclei controlling horizontal but not vertical image stabilization. Moreover, the forward tuned On-Off DSGCs appear physiologically and molecularly distinct from all previously genetically identified On-Off DSGCs. These data begin to clarify the cell types and circuits underlying image stabilization during self-motion, and they support an unexpected diversity of DSGC subtypes.

  View details for DOI 10.1523/JNEUROSCI.2778-13.2013

  View details for Web of Science ID 000327019100027

  View details for PubMedID 24198370

  View details for PubMedCentralID PMC3818553

• Diverse Visual Features Encoded in Mouse Lateral Geniculate Nucleus JOURNAL OF NEUROSCIENCE Piscopo, D. M., El-Danaf, R. N., Huberman, A. D., Niell, C. M. 2013; 33 (11): 4642-4656

  Abstract

  The thalamus is crucial in determining the sensory information conveyed to cortex. In the visual system, the thalamic lateral geniculate nucleus (LGN) is generally thought to encode simple center-surround receptive fields, which are combined into more sophisticated features in cortex, such as orientation and direction selectivity. However, recent evidence suggests that a more diverse set of retinal ganglion cells projects to the LGN. We therefore used multisite extracellular recordings to define the repertoire of visual features represented in the LGN of mouse, an emerging model for visual processing. In addition to center-surround cells, we discovered a substantial population with more selective coding properties, including direction and orientation selectivity, as well as neurons that signal absence of contrast in a visual scene. The direction and orientation selective neurons were enriched in regions that match the termination zones of direction selective ganglion cells from the retina, suggesting a source for their tuning. Together, these data demonstrate that the mouse LGN contains a far more elaborate representation of the visual scene than current models posit. These findings should therefore have a significant impact on our understanding of the computations performed in mouse visual cortex.

  View details for DOI 10.1523/JNEUROSCI.5187-12.2013

  View details for Web of Science ID 000316119200003

  View details for PubMedID 23486939

• Transsynaptic Tracing with Vesicular Stomatitis Virus Reveals Novel Retinal Circuitry JOURNAL OF NEUROSCIENCE Beier, K. T., Borghuis, B. G., El-Danaf, R. N., Huberman, A. D., Demb, J. B., Cepko, C. L. 2013; 33 (1): 35-51

  Abstract

  The use of neurotropic viruses as transsynaptic tracers was first described in the 1960s, but only recently have such viruses gained popularity as a method for labeling neural circuits. The development of retrograde monosynaptic tracing vectors has enabled visualization of the presynaptic sources onto defined sets of postsynaptic neurons. Here, we describe the first application of a novel viral tracer, based on vesicular stomatitis virus (VSV), which directs retrograde transsynaptic viral spread between defined cell types. We use this virus in the mouse retina to show connectivity between starburst amacrine cells (SACs) and their known synaptic partners, direction-selective retinal ganglion cells, as well as to discover previously unknown connectivity between SACs and other retinal ganglion cell types. These novel connections were confirmed using physiological recordings. VSV transsynaptic tracing enables cell type-specific dissection of neural circuitry and can reveal synaptic relationships among neurons that are otherwise obscured due to the complexity and density of neuropil.

  View details for DOI 10.1523/JNEUROSCI.0245-12.2013

  View details for Web of Science ID 000313046500006

  View details for PubMedID 23283320

• Wiring visual circuits, one eye at a time NATURE NEUROSCIENCE El Danaf, R. N., Huberman, A. D. 2012; 15 (2): 172-174

  View details for Web of Science ID 000299603500002

  View details for PubMedID 22281710

• Visual Cognition: Rats Compare Shapes Among the Crowd CURRENT BIOLOGY Cruz-Martin, A., Huberman, A. D. 2012; 22 (1): R18-R20

  Abstract

  Rats can discriminate simple shapes visually, even if they are moved around, made smaller, or partially covered up; the strategy they use may help shed light on human brain mechanisms for discriminating complex features, such as faces.

  View details for DOI 10.1016/j.cub.2011.11.047

  View details for Web of Science ID 000299144200009

  View details for PubMedID 22240473

• What can mice tell us about how vision works? TRENDS IN NEUROSCIENCES Huberman, A. D., Niell, C. M. 2011; 34 (9): 464-473

  Abstract

  Understanding the neural basis of visual perception is a long-standing fundamental goal of neuroscience. Historically, most vision studies were carried out on humans, macaques and cats. Over the past 5 years, however, a growing number of researchers have begun using mice to parse the mechanisms underlying visual processing; the rationale is that, despite having relatively poor acuity, mice are unmatched in terms of the variety and sophistication of tools available to label, monitor and manipulate specific cell types and circuits. In this review, we discuss recent advances in understanding the mouse visual system at the anatomical, receptive field and perceptual level, focusing on the opportunities and constraints those features provide toward the goal of understanding how vision works.

  View details for DOI 10.1016/j.tins.2011.07.002

  View details for Web of Science ID 000294941300003

  View details for PubMedID 21840069

• Cadherin-6 Mediates Axon-Target Matching in a Non-Image-Forming Visual Circuit NEURON Osterhout, J. A., Josten, N., Yamada, J., Pan, F., Wu, S., Nguyen, P. L., Panagiotakos, G., Inoue, Y. U., Egusa, S. F., Volgyi, B., Inoue, T., Bloomfield, S. A., Barres, B. A., Berson, D. M., Feldheim, D. A., Huberman, A. D. 2011; 71 (4): 632-639

  Abstract

  Neural circuits consist of highly precise connections among specific types of neurons that serve a common functional goal. How neurons distinguish among different synaptic targets to form functionally precise circuits remains largely unknown. Here, we show that during development, the adhesion molecule cadherin-6 (Cdh6) is expressed by a subset of retinal ganglion cells (RGCs) and also by their targets in the brain. All of the Cdh6-expressing retinorecipient nuclei mediate non-image-forming visual functions. A screen of mice expressing GFP in specific subsets of RGCs revealed that Cdh3-RGCs which also express Cdh6 selectively innervate Cdh6-expressing retinorecipient targets. Moreover, in Cdh6-deficient mice, the axons of Cdh3-RGCs fail to properly innervate their targets and instead project to other visual nuclei. These findings provide functional evidence that classical cadherins promote mammalian CNS circuit development by ensuring that axons of specific cell types connect to their appropriate synaptic targets.

  View details for DOI 10.1016/j.neuron.2011.07.006

  View details for Web of Science ID 000294521600009

  View details for PubMedID 21867880

  View details for PubMedCentralID PMC3513360

• Pathway-Specific Genetic Attenuation of Glutamate Release Alters Select Features of Competition-Based Visual Circuit Refinement NEURON Koch, S. M., Dela Cruz, C. G., Hnasko, T. S., Edwards, R. H., Huberman, A. D., Ullian, E. M. 2011; 71 (2): 235-242

  Abstract

  A hallmark of mammalian neural circuit development is the refinement of initially imprecise connections by competitive activity-dependent processes. In the developing visual system retinal ganglion cell (RGC) axons from the two eyes undergo activity-dependent competition for territory in the dorsal lateral geniculate nucleus (dLGN). The direct contributions of synaptic transmission to this process, however, remain unclear. We used a genetic approach to reduce glutamate release selectively from ipsilateral-projecting RGCs and found that their release-deficient axons failed to exclude competing axons from the ipsilateral eye territory in the dLGN. Nevertheless, the release-deficient axons consolidated and maintained their normal amount of dLGN territory, even in the face of fully active competing axons. These results show that during visual circuit refinement glutamatergic transmission plays a direct role in excluding competing axons from inappropriate target regions, but they argue that consolidation and maintenance of axonal territory are largely insensitive to alterations in synaptic activity levels.

  View details for DOI 10.1016/j.neuron.2011.05.045

  View details for Web of Science ID 000293433900008

  View details for PubMedID 21791283

• Transgenic Mice Reveal Unexpected Diversity of On-Off Direction-Selective Retinal Ganglion Cell Subtypes and Brain Structures Involved in Motion Processing JOURNAL OF NEUROSCIENCE Rivlin-Etzion, M., Zhou, K., Wei, W., Elstrott, J., Nguyen, P. L., Barres, B. A., Huberman, A. D., Feller, M. B. 2011; 31 (24): 8760-8769

  Abstract

  On-Off direction-selective retinal ganglion cells (DSGCs) encode the axis of visual motion. They respond strongly to an object moving in a preferred direction and weakly to an object moving in the opposite, "null," direction. Historically, On-Off DSGCs were classified into four subtypes according to their directional preference (anterior, posterior, superior, or inferior). Here, we compare two genetically identified populations of On-Off DSGCs: dopamine receptor 4 (DRD4)-DSGCs and thyrotropin-releasing hormone receptor (TRHR)-DSGCs. We find that although both populations are tuned for posterior motion, they can be distinguished by a variety of physiological and anatomical criteria. First, the directional tuning of TRHR-DSGCs is broader than that of DRD4-DSGCs. Second, whereas both populations project similarly to the dorsal lateral geniculate nucleus, they project differently to the ventral lateral geniculate nucleus and the superior colliculus. Moreover, TRHR-DSGCs, but not DRD4-DSGCs, also project to the zona incerta, a thalamic area not previously known to receive direction-tuned visual information. Our findings reveal unexpected diversity among mouse On-Off DSGC subtypes that uniquely process and convey image motion to the brain.

  View details for DOI 10.1523/JNEUROSCI.0564-11.2011

  View details for Web of Science ID 000291642800009

  View details for PubMedID 21677160

  View details for PubMedCentralID PMC3139540

• The Down Syndrome Critical Region Regulates Retinogeniculate Refinement JOURNAL OF NEUROSCIENCE Blank, M., Fuerst, P. G., Stevens, B., Nouri, N., Kirkby, L., Warrier, D., Barres, B. A., Feller, M. B., Huberman, A. D., Burgess, R. W., Garner, C. C. 2011; 31 (15): 5764-5776

  Abstract

  Down syndrome (DS) is a developmental disorder caused by a third chromosome 21 in humans (Trisomy 21), leading to neurological deficits and cognitive impairment. Studies in mouse models of DS suggest that cognitive deficits in the adult are associated with deficits in synaptic learning and memory mechanisms, but it is unclear whether alterations in the early wiring and refinement of neuronal circuits contribute to these deficits. Here, we show that early developmental refinement of visual circuits is perturbed in mouse models of Down syndrome. Specifically, we find excessive eye-specific segregation of retinal axons in the dorsal lateral geniculate nucleus. Indeed, the degree of refinement scales with defects in the "Down syndrome critical region" (DSCR) in a dose-dependent manner. We further identify Dscam (Down syndrome cell adhesion molecule), a gene within the DSCR, as a regulator of eye-specific segregation of retinogeniculate projections. Although Dscam is not the sole gene in the DSCR contributing to enhanced refinement in trisomy, Dscam dosage clearly regulates cell spacing and dendritic fasciculation in a specific class of retinal ganglion cells. Thus, altered developmental refinement of visual circuits that occurs before sensory experience is likely to contribute to visual impairment in individuals with Down syndrome.

  View details for DOI 10.1523/JNEUROSCI.6015-10.2011

  View details for Web of Science ID 000289472400026

  View details for PubMedID 21490218

  View details for PubMedCentralID PMC3230532

• Emergence of Lamina-Specific Retinal Ganglion Cell Connectivity by Axon Arbor Retraction and Synapse Elimination JOURNAL OF NEUROSCIENCE Cheng, T., Liu, X., Faulkner, R. L., Stephan, A. H., Barres, B. A., Huberman, A. D., Cheng, H. 2010; 30 (48): 16376-16382

  Abstract

  Throughout the nervous system, neurons restrict their connections to specific depths or "layers" of their targets to constrain the type and number of synapses they make. Despite the importance of lamina-specific synaptic connectivity, the mechanisms that give rise to this feature in mammals remain poorly understood. Here we examined the cellular events underlying the formation of lamina-specific retinal ganglion cell (RGC) axonal projections to the superior colliculus (SC) of the mouse. By combining a genetically encoded marker of a defined RGC subtype (OFF-αRGCs) with serial immunoelectron microscopy, we resolved the ultrastructure of axon terminals fated for laminar stabilization versus those fated for removal. We found that OFF-αRGCs form synapses across the full depth of the retinorecipient SC before undergoing lamina-specific arbor retraction and synapse elimination to arrive at their mature, restricted pattern of connectivity. Interestingly, we did not observe evidence of axon degeneration or glia-induced synapse engulfment during this process. These findings indicate that lamina-specific visual connections are generated through the selective stabilization of correctly targeted axon arbors and suggest that the decision to maintain or eliminate an axonal projection reflects the molecular compatibility of presynaptic and postsynaptic neurons at a given laminar depth.

  View details for DOI 10.1523/JNEUROSCI.3455-10.2010

  View details for Web of Science ID 000284999900031

  View details for PubMedID 21123583

  View details for PubMedCentralID PMC3073606

• Molecular and Cellular Mechanisms of Lamina-specific Axon Targeting COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY Huberman, A. D., Clandinin, T. R., Baier, H. 2010; 2 (3)

  Abstract

  The specificity of synaptic connections is directly related to the functional integrity of neural circuits. Long-range axon guidance and topographic mapping mechanisms bring axons into spatial proximity of target cells and thus limit the number of potential synaptic partners. Synaptic specificity is then achieved by extracellular short-range guidance cues and cell-surface recognition cues. Neural activity may enhance the precision and strength of specific circuit connections. Here, we focus on one of the final steps of synaptic matchmaking: the targeting of synaptic layers and the mutual recognition of axons and dendrites within these layers.

  View details for DOI 10.1101/cshperspect.a001743

  View details for Web of Science ID 000279881700009

  View details for PubMedID 20300211

  View details for PubMedCentralID PMC2829955

• MILESTONES AND MECHANISMS FOR GENERATING SPECIFIC SYNAPTIC CONNECTIONS BETWEEN THE EYES AND THE BRAIN INVERTEBRATE AND VERTEBRATE EYE DEVELOPMENT Josten, N. J., Huberman, A. D. 2010; 93: 229-259

  Abstract

  All information about the visual world is conveyed to the brain by a single type of neurons at the back of the eye called retinal ganglion cells (RGCs). Understanding how RGC axons locate and wire up with their targets is therefore critical to understanding visual development. In recent years, several important technological and conceptual advances have been made in this area, and yet, many fundamental questions remain unanswered. Indeed, while much is now known about how RGC axons pathfind at the optic chiasm and form retinotopic maps within their targets, how RGCs select their overall targets in the first place is poorly understood. Moreover, the signals that direct mammalian RGC axons to their appropriate layer within those targets remain unknown. The recent advent of genetic tools to selectively label and manipulate defined groups of RGCs is starting to provide a way to resolve these and other important questions about RGC wiring specificity. This field is therefore positioned to reveal new principles of visual circuit development that no doubt will extend to other regions of the CNS.

  View details for DOI 10.1016/S0070-2153(10)93008-1

  View details for Web of Science ID 000283820000008

  View details for PubMedID 20959168

• Genetic Identification of an On-Off Direction-Selective Retinal Ganglion Cell Subtype Reveals a Layer-Specific Subcortical Map of Posterior Motion NEURON Huberman, A. D., Wei, W., Elstrott, J., Stafford, B. K., Feller, M. B., Barres, B. A. 2009; 62 (3): 327-334

  Abstract

  Motion detection is an essential component of visual processing. On-Off direction-selective retinal ganglion cells (On-Off DSGCs) detect objects moving along specific axes of the visual field due to their precise retinal circuitry. The brain circuitry of On-Off DSGCs, however, is largely unknown. We report a mouse with GFP expressed selectively by the On-Off DSGCs that detect posterior motion (On-Off pDSGCs), allowing two-photon targeted recordings of their light responses and delineation of their complete map of central connections. On-Off pDSGCs project exclusively to the dorsal lateral geniculate nucleus and superior colliculus and in both targets form synaptic lamina that are separate from a lamina corresponding to non-DSGCs. Thus, individual On-Off DSGC subtypes are molecularly distinct and establish circuits that map specific qualities of directional motion to dedicated subcortical areas. This suggests that each RGC subtype represents a unique parallel pathway whose synaptic specificity in the retina is recapitulated in central targets.

  View details for DOI 10.1016/j.neuron.2009.04.014

  View details for Web of Science ID 000266146100005

  View details for PubMedID 19447089

  View details for PubMedCentralID PMC3140054

• Architecture and activity-mediated refinement of axonal projections from a mosaic of genetically identified retinal ganglion cells NEURON Huberman, A. D., Manu, M., Koch, S. M., Susman, M. W., Lutz, A. B., Ullian, E. M., Baccus, S. A., Barres, B. A. 2008; 59 (3): 425-438

  Abstract

  Our understanding of how mammalian sensory circuits are organized and develop has long been hindered by the lack of genetic markers of neurons with discrete functions. Here, we report a transgenic mouse selectively expressing GFP in a complete mosaic of transient OFF-alpha retinal ganglion cells (tOFF-alphaRGCs). This enabled us to relate the mosaic spacing, dendritic anatomy, and electrophysiology of these RGCs to their complete map of projections in the brain. We find that tOFF-alphaRGCs project exclusively to the superior colliculus (SC) and dorsal lateral geniculate nucleus and are restricted to a specific laminar depth within each of these targets. The axons of tOFF-alphaRGC are also organized into columns in the SC. Both laminar and columnar specificity develop through axon refinement. Disruption of cholinergic retinal waves prevents the emergence of columnar- but not laminar-specific tOFF-alphaRGC connections. Our findings reveal that in a genetically identified sensory map, spontaneous activity promotes synaptic specificity by segregating axons arising from RGCs of the same subtype.

  View details for DOI 10.1016/j.neuron.2008.07.018

  View details for Web of Science ID 000258565500011

  View details for PubMedID 18701068

• Mechanisms underlying development of visual maps and receptive fields ANNUAL REVIEW OF NEUROSCIENCE Huberman, A. D., Feller, M. B., Chapman, B. 2008; 31: 479-509

  Abstract

  Patterns of synaptic connections in the visual system are remarkably precise. These connections dictate the receptive field properties of individual visual neurons and ultimately determine the quality of visual perception. Spontaneous neural activity is necessary for the development of various receptive field properties and visual feature maps. In recent years, attention has shifted to understanding the mechanisms by which spontaneous activity in the developing retina, lateral geniculate nucleus, and visual cortex instruct the axonal and dendritic refinements that give rise to orderly connections in the visual system. Axon guidance cues and a growing list of other molecules, including immune system factors, have also recently been implicated in visual circuit wiring. A major goal now is to determine how these molecules cooperate with spontaneous and visually evoked activity to give rise to the circuits underlying precise receptive field tuning and orderly visual maps.

  View details for DOI 10.1146/annurev.neuro.31.060407.125533

  View details for Web of Science ID 000257992200020

  View details for PubMedID 18558864

  View details for PubMedCentralID PMC2655105

• The classical complement cascade mediates CNS synapse elimination CELL Stevens, B., Allen, N. J., Vazquez, L. E., Howell, G. R., Christopherson, K. S., Nouri, N., Micheva, K. D., Mehalow, A. K., Huberman, A. D., Stafford, B., Sher, A., Litke, A. M., Lambris, J. D., Smith, S. J., John, S. W., Barres, B. A. 2007; 131 (6): 1164-1178

  Abstract

  During development, the formation of mature neural circuits requires the selective elimination of inappropriate synaptic connections. Here we show that C1q, the initiating protein in the classical complement cascade, is expressed by postnatal neurons in response to immature astrocytes and is localized to synapses throughout the postnatal CNS and retina. Mice deficient in complement protein C1q or the downstream complement protein C3 exhibit large sustained defects in CNS synapse elimination, as shown by the failure of anatomical refinement of retinogeniculate connections and the retention of excess retinal innervation by lateral geniculate neurons. Neuronal C1q is normally downregulated in the adult CNS; however, in a mouse model of glaucoma, C1q becomes upregulated and synaptically relocalized in the adult retina early in the disease. These findings support a model in which unwanted synapses are tagged by complement for elimination and suggest that complement-mediated synapse elimination may become aberrantly reactivated in neurodegenerative disease.

  View details for DOI 10.1016/j.cell.2007.10.036

  View details for PubMedID 18083105

• Mechanisms of eye-specific visual circuit development CURRENT OPINION IN NEUROBIOLOGY Huberman, A. D. 2007; 17 (1): 73-80

  Abstract

  Eye-specific visual connections are a prominent model system for exploring how precise circuits develop in the CNS and, in particular, for addressing the role of neural activity in synapse elimination and axon refinement. Recent experiments have identified the features of spontaneous retinal activity that mediate eye-specific retinogeniculate segregation, the synaptic events associated with this process, and the importance of axon guidance cues for organizing the overall layout of eye-specific maps. The classic model of ocular dominance column development, in which spontaneous retinal activity plays a crucial role, has also gained new support. Although many outstanding questions remain, the mechanisms that instruct eye-specific circuit development are becoming clear.

  View details for DOI 10.1016/j.conb.2007.01.005

  View details for Web of Science ID 000244771100011

  View details for PubMedID 17254766

• Spontaneous retinal activity mediates development of ocular dominance columns and binocular receptive fields in V1 NEURON Huberman, A. D., Speer, C. M., Chapman, B. 2006; 52 (2): 247-254

  Abstract

  The mechanisms that give rise to ocular dominance columns (ODCs) during development are controversial. Early experiments indicated a key role for retinal activity in ODC formation. However, later studies showed that in those early experiments, the retinal activity perturbation was initiated after ODCs had already formed. Moreover, recent studies concluded that early eye removals do not impact ODC segregation. Here we blocked spontaneous retinal activity during the very early stages of ODC development. This permanently disrupted the anatomical organization of ODCs and led to a dramatic increase in receptive field size for binocular cells in primary visual cortex. Our data suggest that early spontaneous retinal activity conveys crucial information about whether thalamocortical axons represent one or the other eye and that this activity mediates binocular competition important for shaping receptive fields in primary visual cortex.

  View details for DOI 10.1016/j.neuron.2006.07.028

  View details for Web of Science ID 000241799900006

  View details for PubMedID 17046688

  View details for PubMedCentralID PMC2647846

• Neuronal pentraxins mediate synaptic refinement in the developing visual system JOURNAL OF NEUROSCIENCE Bjartmar, L., Huberman, A. D., Ullian, E. M., Renteria, R. C., Liu, X., Xu, W., Prezioso, J., Susman, M. W., Stellwagen, D., Stokes, C. C., Cho, R., Worley, P., Malenka, R. C., Ball, S., Peachey, N. S., Copenhagen, D., Chapman, B., Nakamoto, M., Barres, B. A., Perin, M. S. 2006; 26 (23): 6269-6281

  Abstract

  Neuronal pentraxins (NPs) define a family of proteins that are homologous to C-reactive and acute-phase proteins in the immune system and have been hypothesized to be involved in activity-dependent synaptic plasticity. To investigate the role of NPs in vivo, we generated mice that lack one, two, or all three NPs. NP1/2 knock-out mice exhibited defects in the segregation of eye-specific retinal ganglion cell (RGC) projections to the dorsal lateral geniculate nucleus, a process that involves activity-dependent synapse formation and elimination. Retinas from mice lacking NP1 and NP2 had cholinergically driven waves of activity that occurred at a frequency similar to that of wild-type mice, but several other parameters of retinal activity were altered. RGCs cultured from these mice exhibited a significant delay in functional maturation of glutamatergic synapses. Other developmental processes, such as pathfinding of RGCs at the optic chiasm and hippocampal long-term potentiation and long-term depression, appeared normal in NP-deficient mice. These data indicate that NPs are necessary for early synaptic refinements in the mammalian retina and dorsal lateral geniculate nucleus. We speculate that NPs exert their effects through mechanisms that parallel the known role of short pentraxins outside the CNS.

  View details for DOI 10.1523/jneurosci.4212-05.2006

  View details for Web of Science ID 000238174600017

  View details for PubMedID 16763034

  View details for PubMedCentralID PMC2579897

• Dynamics of spontaneous activity in the fetal macaque retina during development of retinogeniculate pathways JOURNAL OF NEUROSCIENCE Warland, D. K., Huberman, A. D., Chalupa, L. M. 2006; 26 (19): 5190-5197

  Abstract

  Correlated spontaneous activity in the form of retinal "waves" has been observed in a wide variety of developing animals, but whether retinal waves occur in the primate has not been determined previously. To address this issue, we recorded from isolated retinas using multielectrode arrays at six fetal ages: embryonic day 51 (E51), E55, E60, E67, E71, and E76. These recordings revealed that the fetal monkey retina is essentially silent at E51 and E55, with only few cells firing on rare occasions and without any obvious spatial or temporal order. Because previous work has shown that the magnocellular and parvocellular subdivisions of the dorsal lateral geniculate are selectively innervated during this early period, our results suggest that this process is unlikely to be regulated by retinal activity. Highly structured retinal waves were first observed at E60, >1 week before the segregation of eye-specific retinal dorsal lateral geniculate nucleus projections commences. The incidence of such waves decreased rapidly and progressively during the developmental period (E67-E76) when segregated eye-specific projections become established. Our findings indicate that retinal waves first occur in the fetal monkey at a remarkably early stage of development, >100 d before birth, and that this activity undergoes rapid changes in salient properties when eye-specific retinogeniculate projections are being formed.

  View details for DOI 10.1523/JNEUROSCI.0328-06.2006

  View details for Web of Science ID 000237450300021

  View details for PubMedID 16687510

• Nob mice wave goodbye to eye-specific segregation NEURON Huberman, A. D. 2006; 50 (2): 175-177

  Abstract

  Spontaneous retinal activity is necessary to establish and maintain eye-specific projections to the LGN, but whether the spatial and temporal structure of this activity is important remains unclear. A new study by Demas et al. in the current issue of Neuron shows that when the frequency of spontaneous retinal waves is increased and waves abnormally persist past eye opening, eye-specific projections to the LGN desegregate. These results provide important new insight into the mechanisms that drive eye-specific refinement and stabilization.

  View details for DOI 10.1016/j.neuron.2006.04.006

  View details for Web of Science ID 000237176700002

  View details for PubMedID 16630826

• Target-derived cues instruct synaptic differentiation JOURNAL OF NEUROSCIENCE Huberman, A. D. 2006; 26 (4): 1063-1064

  View details for DOI 10.1523/JNEUROSCI.4456-05.2006

  View details for Web of Science ID 000234896200002

  View details for PubMedID 16436591

• Ephrin-As mediate targeting of eye-specific projections to the lateral geniculate nucleus NATURE NEUROSCIENCE Huberman, A. D., Murray, K. D., Warland, D. K., Feldheim, D. A., Chapman, B. 2005; 8 (8): 1013-1021

  Abstract

  Axon guidance cues contributing to the development of eye-specific visual projections to the lateral geniculate nucleus (LGN) have not previously been identified. Here we show that gradients of ephrin-As and their receptors (EphAs) direct retinal ganglion cell (RGC) axons from the two eyes into their stereotyped pattern of layers in the LGN. Overexpression of EphAs in ferret RGCs using in vivo electroporation induced axons from both eyes to misproject within the LGN. The effects of EphA overexpression were competition-dependent and restricted to the early postnatal period. These findings represent the first demonstration of eye-specific pathfinding mediated by axon guidance cues and, taken with other reports, indicate that ephrin-As can mediate several mapping functions within individual target structures.

  View details for DOI 10.1038/nn1505

  View details for Web of Science ID 000230760200012

  View details for PubMedID 16025110

  View details for PubMedCentralID PMC2652399

• Early and rapid targeting of eye-specific axonal projections to the dorsal lateral geniculate nucleus in the fetal macaque JOURNAL OF NEUROSCIENCE Huberman, A. D., Dehay, C., Berland, M., Chalupa, L. M., Kennedy, H. 2005; 25 (16): 4014-4023

  Abstract

  The emergence of eye-specific axonal projections to the dorsal lateral geniculate nucleus (dLGN) is a well established model system for exploring the mechanisms underlying afferent targeting during development. Using modern tract tracing methods, we examined the development of this feature in the macaque, an Old World Primate with a visual system similar to that of humans. Cholera toxin beta fragment conjugated to Alexa 488 was injected into the vitreous of one eye, and CTbeta conjugated to Alexa 594 into the other eye of embryos at known gestational ages. On embryonic day 69 (E69), which is approximately 100 d before birth, inputs from the two eyes were extensively intermingled in the dLGN. However, even at this early age, portions of the dLGN were preferentially innervated by the right or left eye, and segregation is complete within the dorsalmost layers 5 and 6. By E78, eye-specific segregation is clearly established throughout the parvocellular division of the dLGN, and substantial ocular segregation is present in the magnocellular division. By E84, segregation of left and right eye axons is essentially complete, and the six eye-specific domains that characterize the mature macaque dLGN are clearly discernable. These findings reveal that targeting of eye-specific axonal projections in the macaque occurs much earlier and more rapidly than previously reported. This segregation process is completed before the reported onset of ganglion cell axon loss and retino-dLGN synapse elimination, suggesting that, in the primate, eye-specific targeting occurs independent of traditional forms of synaptic plasticity.

  View details for DOI 10.1523/JNEUROSCI.4292-04.2005

  View details for Web of Science ID 000228542600003

  View details for PubMedID 15843603

  View details for PubMedCentralID PMC2709237

• Decoupling eye-specific segregation from lamination in the lateral geniculate nucleus JOURNAL OF NEUROSCIENCE Huberman, A. D., Stellwagen, D., Chapman, B. 2002; 22 (21): 9419-9429

  Abstract

  To determine whether there is a critical period for development of eye-specific layers in the lateral geniculate nucleus (LGN), we prevented the normal segregation of retinogeniculate afferents and then allowed an extended period of time for recovery. After recovery, both anatomy and physiology revealed strictly nonoverlapping territories of input from the two eyes. However, the normal stereotyped pattern of eye-specific afferent and cellular layers never developed. Instead, the eye-specific territories of afferent input emerged as variable and disorganized patches with no corresponding interlaminar spaces in the LGN. These findings reveal a critical period for coordinating the development of three processes in the LGN: the segregation of afferents from the two eyes, the spatial organization of those afferents into layers, and the alignment of postsynaptic cytoarchitecture with the afferent inputs. We also assessed the physiological consequences of preventing normal lamination and found normal single-cell responses and topographic representation of visual space in the LGN. Clusters of ON-center and OFF-center LGN cells were segregated from one another as in normal animals. However, the organization of ON and OFF sublaminas in the treated animals was disrupted.

  View details for Web of Science ID 000179031600032

  View details for PubMedID 12417667

  View details for PubMedCentralID PMC2662346