Honors & Awards


  • Cardiovascular Imaging NIH T32 Postdoctoral Fellowship, National Institute of Biomedical Imaging and Bioengineering (NHLBI) (February 2021- Present)
  • Outstanding Graduate Student Award for Research in Life Sciences, Physical Sciences, and Engineering, Northeastern University (April 2020)
  • Most First-author Peer-reviewed Journal Publications Award in 2019, College of Engineering, Northeastern University (April 2020)
  • Most Cited Publications Award in 2019, College of Engineering, Northeastern University (April 2020)
  • DCF Fellowship Award, College of Engineering, Northeastern University (April 2020)
  • Best poster award (1st place), PhD poster competition @National Engineers Week, College of Engineering, Northeastern University (February 2020)
  • Travel Award for 2019 AIChE Annual Meeting, "Nanoengineering of an Electroconductive Cardiac Patch", American Institute of Chemical Engineers (AIChE), Orlando, FL (2019)
  • Outstanding Reviewer Certificate, Elsevier Publisher (August 2018)

Boards, Advisory Committees, Professional Organizations


  • Editorial Board, Nature Scientific Reports (2021 - Present)
  • Associate Editor-in-Chief, International Journal of Nanomedicine (IJN), Dove Medical Press/ T&F (2020 - Present)
  • Editorial Board, Journal of Nanostructure in Chemistry, Springer Nature (2021 - Present)
  • Editorial Board, Journal of Functional Biomaterials- MDPI (2021 - Present)
  • Special Issue Editor, "3D Printing in Heart and Cardiovascular Disease", Reviews in Cardiovascular Medicine (2021 - Present)
  • Special Issue Editor, "Emerging Nanotechnology Enabled Theragnostic Approaches in Malignancies and Microbial Infections", International Journal of Molecular Sciences (IJMS), MDPI (2021 - Present)
  • Topic Editor, International Journal of Molecular Sciences (IJMS), MDPI (2021 - Present)
  • Special Issue Editor, "Nanoengineered Materials for Biomedical Applications", Journal of Functional Biomaterials, MDPI (2021 - Present)
  • Editorial Board, Journal of Stem Cell Research & Therapy (2021 - Present)
  • Special Issue Guest Editor, "Nanobiosensors: From Fabrication to Diagnostic, Therapeutic, and Theragnostic Applications", Sensors, MDPI (2021 - Present)
  • Review Editor for Pharmacology of Anti Cancer Drugs, Frontiers in Pharmacology & Frontiers in Oncology (2021 - Present)
  • Topic Editor, Processes, MDPI (2021 - Present)
  • Editorial Board, Annals of Materials Science & Engineering, Austin Publishing Group (2021 - Present)
  • Guest Editor, Special Issue "Molecular and Cellular Nanobiotechnology", BIOCELL (2021 - Present)
  • Member & subcommittee of Nanoneuroscience/nanomedicine, Society for Brain Mapping and Therapeutics (SBMT) (2020 - Present)
  • Editorial Board, Journal of Regenerative Biology and Medicine (2020 - Present)
  • Editorial Board, Journal of Next Generation Sequencing & Applications (2020 - Present)

Professional Education


  • PhD, Northeastern University, Chemical Engineering (in Aspects of Biomedical Engineering and Biotechnology) (2020)
  • MSc, University of Tehran, Materials Science and Engineering (2013)
  • BSc, University of Tehran, Materials Science and Engineering (2011)

Stanford Advisors


All Publications


  • Theoretical Encapsulation of Fluorouracil (5-FU) Anti-Cancer Chemotherapy Drug into Carbon Nanotubes (CNT) and Boron Nitride Nanotubes (BNNT). Molecules (Basel, Switzerland) Zarghami Dehaghani, M., Yousefi, F., Sajadi, S. M., Tajammal Munir, M., Abida, O., Habibzadeh, S., Mashhadzadeh, A. H., Rabiee, N., Mostafavi, E., Saeb, M. R. 2021; 26 (16)

    Abstract

    INTRODUCTION: Chemotherapy with anti-cancer drugs is considered the most common approach for killing cancer cells in the human body. However, some barriers such as toxicity and side effects would limit its usage. In this regard, nano-based drug delivery systems have emerged as cost-effective and efficient for sustained and targeted drug delivery. Nanotubes such as carbon nanotubes (CNT) and boron nitride nanotubes (BNNT) are promising nanocarriers that provide the cargo with a large inner volume for encapsulation. However, understanding the insertion process of the anti-cancer drugs into the nanotubes and demonstrating drug-nanotube interactions starts with theoretical analysis.METHODS: First, interactions parameters of the atoms of 5-FU were quantified from the DREIDING force field. Second, the storage capacity of BNNT (8,8) was simulated to count the number of drugs 5-FU encapsulated inside the cavity of the nanotubes. In terms of the encapsulation process of the one drug 5-FU into nanotubes, it was clarified that the drug 5-FU was more rapidly adsorbed into the cavity of the BNNT compared with the CNT due to the higher van der Waals (vdW) interaction energy between the drug and the BNNT.RESULTS: The obtained values of free energy confirmed that the encapsulation process of the drug inside the CNT and BNNT occurred spontaneously with the free energies of -14 and -25 kcal·mol-1, respectively.DISCUSSION: However, the lower value of the free energy in the system containing the BNNT unraveled more stability of the encapsulated drug inside the cavity of the BNNT comparing the system having CNT. The encapsulation of Fluorouracil (5-FU) anti-cancer chemotherapy drug (commercial name: Adrucil) into CNT (8,8) and BNNT (8,8) with the length of 20 A in an aqueous solution was discussed herein applying molecular dynamics (MD) simulation.

    View details for DOI 10.3390/molecules26164920

    View details for PubMedID 34443508

  • Drug delivery to the anterior segment of the eye: a review of current and future treatment strategies. International journal of pharmaceutics Mofidfar, M., Abdi, B., Ahadian, S., Mostafavi, E., Desai, T. A., Abbasi, F., Sun, Y., Manche, E. E., Ta, C. N., Flowers, C. W. 2021: 120924

    Abstract

    Research in the development of ophthalmic drug formulations and innovative technologies over the past few decades has been directed at improving the penetration of medications delivered to the eye. Currently, approximately 90% of all ophthalmic drug formulations (e.g. liposomes, micelles) are applied as eye drops. The major challenge of topical eye drops is low bioavailability, need for frequent instillation due to the short half-life, poor drug solubility, and potential side effects. Recent research has been focused on improving topical drug delivery devices by increasing ocular residence time, overcoming physiological and anatomical barriers, and developing medical devices and drug formulations to increase the duration of action of the active drugs. Researchers have developed innovative technologies and formulations ranging from sub-micron to macroscopic size such as prodrugs, enhancers, mucus-penetrating particles (MPPs), therapeutic contact lenses, and collagen corneal shields. Another approach towards the development of effective topical drug delivery is embedding therapeutic formulations in microdevices designed for sustained release of the active drugs. The goal is to optimize the delivery of ophthalmic medications by achieving high drug concentration with prolonged duration of action that is convenient for patients to administer.

    View details for DOI 10.1016/j.ijpharm.2021.120924

    View details for PubMedID 34324989

  • Advances in 3D-Printed Surface-Modified Ca-Si Bioceramic Structures and Their Potential for Bone Tumor Therapy. Materials (Basel, Switzerland) Truong, L. B., Medina Cruz, D., Mostafavi, E., O'Connell, C. P., Webster, T. J. 2021; 14 (14)

    Abstract

    Bioceramics such as calcium silicate (Ca-Si), have gained a lot of interest in the biomedical field due to their strength, osteogenesis capability, mechanical stability, and biocompatibility. As such, these materials are excellent candidates to promote bone and tissue regeneration along with treating bone cancer. Bioceramic scaffolds, functionalized with appropriate materials, can achieve desirable photothermal effects, opening up a bifunctional approach to osteosarcoma treatments-simultaneously killing cancerous cells while expediting healthy bone tissue regeneration. At the same time, they can also be used as vehicles and cargo structures to deliver anticancer drugs and molecules in a targeted manner to tumorous tissue. However, the traditional synthesis routes for these bioceramic scaffolds limit the macro-, micro-, and nanostructures necessary for maximal benefits for photothermal therapy and drug delivery. Therefore, a different approach to formulate bioceramic scaffolds has emerged in the form of 3D printing, which offers a sustainable, highly reproducible, and scalable method for the production of valuable biomedical materials. Here, calcium silicate (Ca-Si) is reviewed as a novel 3D printing base material, functionalized with highly photothermal materials for osteosarcoma therapy and drug delivery platforms. Consequently, this review aims to detail advances made towards functionalizing 3D-printed Ca-Si and similar bioceramic scaffold structures as well as their resulting applications for various aspects of tumor therapy, with a focus on the external surface and internal dispersion functionalization of the scaffolds.

    View details for DOI 10.3390/ma14143844

    View details for PubMedID 34300763

  • Metal-Organic Frameworks-Based Nanomaterials for Drug Delivery. Materials (Basel, Switzerland) Saeb, M. R., Rabiee, N., Mozafari, M., Mostafavi, E. 2021; 14 (13)

    Abstract

    The composition and topology of metal-organic frameworks (MOFs) are exceptionally tailorable; moreover, they are extremely porous and represent an excellent Brunauer-Emmett-Teller (BET) surface area (3000-6000 m2·g-1). Nanoscale MOFs (NMOFs), as cargo nanocarriers, have increasingly attracted the attention of scientists and biotechnologists during the past decade, in parallel with the evolution in the use of porous nanomaterials in biomedicine. Compared to other nanoparticle-based delivery systems, such as porous nanosilica, nanomicelles, and dendrimer-encapsulated nanoparticles, NMOFs are more flexible, have a higher biodegradability potential, and can be more easily functionalized to meet the required level of host-guest interactions, while preserving a larger and fully adjustable pore window in most cases. Due to these unique properties, NMOFs have the potential to carry anticancer cargos. In contrast to almost all porous materials, MOFs can be synthesized in diverse morphologies, including spherical, ellipsoidal, cubic, hexagonal, and octahedral, which facilitates the acceptance of various drugs and genes.

    View details for DOI 10.3390/ma14133652

    View details for PubMedID 34208958

  • Co-Loading of Cisplatin and Methotrexate in Nanoparticle-Based PCL-PEG System Enhances Lung Cancer Chemotherapy Effects JOURNAL OF CLUSTER SCIENCE Akbari, E., Mousazadeh, H., Hanifehpour, Y., Mostafavi, E., Gorabi, A., Nejati, K., Keyhanvar, P., Pazoki-Toroudi, H., Mohammadhosseini, M., Akbarzadeh, A. 2021
  • Selenium Nanomaterials to Combat Antimicrobial Resistance MOLECULES Truong, L. B., Medina-Cruz, D., Mostafavi, E., Rabiee, N. 2021; 26 (12)

    Abstract

    The rise of antimicrobial resistance to antibiotics (AMR) as a healthcare crisis has led to a tremendous social and economic impact, whose damage poses a significant threat to future generations. Current treatments either are less effective or result in further acquired resistance. At the same time, several new antimicrobial discovery approaches are expensive, slow, and relatively poorly equipped for translation into the clinical world. Therefore, the use of nanomaterials is presented as a suitable solution. In particular, this review discusses selenium nanoparticles (SeNPs) as one of the most promising therapeutic agents based in the nanoscale to treat infections effectively. This work summarizes the latest advances in the synthesis of SeNPs and their progress as antimicrobial agents using traditional and biogenic approaches. While physiochemical methods produce consistent nanostructures, along with shortened processing procedures and potential for functionalization of designs, green or biogenic synthesis represents a quick, inexpensive, efficient, and eco-friendly approach with more promise for tunability and versatility. In the end, the clinical translation of SeNPs faces various obstacles, including uncertain in vivo safety profiles and mechanisms of action and unclear regulatory frameworks. Nonetheless, the promise possessed by these metalloid nanostructures, along with other nanoparticles in treating bacterial infections and slowing down the AMR crisis, are worth exploring.

    View details for DOI 10.3390/molecules26123611

    View details for Web of Science ID 000666179500001

    View details for PubMedID 34204666

    View details for PubMedCentralID PMC8231168

  • Ubiquitin-proteasome system and the role of its inhibitors in cancer therapy. Open biology Aliabadi, F., Sohrabi, B., Mostafavi, E., Pazoki-Toroudi, H., Webster, T. J. 2021; 11 (4): 200390

    Abstract

    Despite all the other cells that have the potential to prevent cancer development and metastasis through tumour suppressor proteins, cancer cells can upregulate the ubiquitin-proteasome system (UPS) by which they can degrade tumour suppressor proteins and avoid apoptosis. This system plays an extensive role in cell regulation organized in two steps. Each step has an important role in controlling cancer. This demonstrates the importance of understanding UPS inhibitors and improving these inhibitors to foster a new hope in cancer therapy. UPS inhibitors, as less invasive chemotherapy drugs, are increasingly used to alleviate symptoms of various cancers in malignant states. Despite their success in reducing the development of cancer with the lowest side effects, thus far, an appropriate inhibitor that can effectively inactivate this system with the least drug resistance has not yet been fully investigated. A fundamental understanding of the system is necessary to fully elucidate its role in causing/controlling cancer. In this review, we first comprehensively investigate this system, and then each step containing ubiquitination and protein degradation as well as their inhibitors are discussed. Ultimately, its advantages and disadvantages and some perspectives for improving the efficiency of these inhibitors are discussed.

    View details for DOI 10.1098/rsob.200390

    View details for PubMedID 33906413

  • Aloe Vera-Mediated Te Nanostructures: Highly Potent Antibacterial Agents and Moderated Anticancer Effects. Nanomaterials (Basel, Switzerland) Medina-Cruz, D., Vernet-Crua, A., Mostafavi, E., Gonzalez, M. U., Martinez, L., Iii, A. D., Kusper, M., Sotelo, E., Gao, M., Geoffrion, L. D., Shah, V., Guisbiers, G., Cholula-Diaz, J. L., Guillermier, C., Khanom, F., Huttel, Y., Garcia-Martin, J. M., Webster, T. J. 2021; 11 (2)

    Abstract

    Cancer and antimicrobial resistance to antibiotics are two of the most worrying healthcare concerns that humanity is facing nowadays. Some of the most promising solutions for these healthcare problems may come from nanomedicine. While the traditional synthesis of nanomaterials is often accompanied by drawbacks such as high cost or the production of toxic by-products, green nanotechnology has been presented as a suitable solution to overcome such challenges. In this work, an approach for the synthesis of tellurium (Te) nanostructures in aqueous media has been developed using aloe vera (AV) extracts as a unique reducing and capping agent. Te-based nanoparticles (AV-TeNPs), with sizes between 20 and 60 nm, were characterized in terms of physicochemical properties and tested for potential biomedical applications. A significant decay in bacterial growth after 24 h was achieved for both Methicillin-resistant Staphylococcus aureus and multidrug-resistant Escherichia coli at a relative low concentration of 5 g/mL, while there was no cytotoxicity towards human dermal fibroblasts after 3 days of treatment. AV-TeNPs also showed anticancer properties up to 72 h within a range of concentrations between 5 and 100 g/mL. Consequently, here, we present a novel and green approach to produce Te-based nanostructures with potential biomedical applications, especially for antibacterial and anticancer applications.

    View details for DOI 10.3390/nano11020514

    View details for PubMedID 33670538

  • Boron Nitride Nanotube as an Antimicrobial Peptide Carrier: A Theoretical Insight. International journal of nanomedicine Zarghami Dehaghani, M., Bagheri, B., Yousefi, F., Nasiriasayesh, A., Hamed Mashhadzadeh, A., Zarrintaj, P., Rabiee, N., Bagherzadeh, M., Fierro, V., Celzard, A., Saeb, M. R., Mostafavi, E. 2021; 16: 1837–47

    Abstract

    Introduction: Nanotube-based drug delivery systems have received considerable attention because of their large internal volume to encapsulate the drug and the ability to penetrate tissues, cells, and bacteria. In this regard, understanding the interaction between the drug and the nanotube to evaluate the encapsulation behavior of the drug in the nanotube is of crucial importance.Methods: In this work, the encapsulation process of the cationic antimicrobial peptide named cRW3 in the biocompatible boron nitride nanotube (BNNT) was investigated under the Canonical ensemble (NVT) by molecular dynamics (MD) simulation.Results: The peptide was absorbed into the BNNT by van der Waals (vdW) interaction between cRW3 and the BNNT, in which the vdW interaction decreased during the simulation process and reached the value of -142.7 kcal·mol-1 at 4 ns.Discussion: The increase in the potential mean force profile of the encapsulated peptide during the pulling process of cRW3 out of the nanotube showed that its insertion into the BNNT occurred spontaneously and that the inserted peptide had the desired stability. The energy barrier at the entrance of the BNNT caused a pause of 0.45 ns when half of the peptide was inside the BNNT during the encapsulation process. Therefore, during this period, the peptide experienced the weakest movement and the smallest conformational changes.

    View details for DOI 10.2147/IJN.S298699

    View details for PubMedID 33692624

  • alpha-Helical Antimicrobial Peptide Encapsulation and Release from Boron Nitride Nanotubes: A Computational Study INTERNATIONAL JOURNAL OF NANOMEDICINE Dehaghani, M., Yousefi, F., Bagheri, B., Seid, F., Mashhadzadeh, A., Rabiee, N., Zarrintaj, P., Mostafavi, E., Saeb, M., Kim, Y. 2021; 16: 4277-4288

    Abstract

    Antimicrobial peptides are potential therapeutics as anti-bacteria, anti-viruses, anti-fungi, or anticancers. However, they suffer from a short half-life and drug resistance which limit their long-term clinical usage.Herein, we captured the encapsulation of antimicrobial peptide HA-FD-13 into boron nitride nanotube (BNNT) (20,20) and its release due to subsequent insertion of BNNT (14,14) with molecular dynamics simulation.The peptide-BNNT (20,20) van der Waals (vdW) interaction energy decreased to -270 kcal·mol-1 at the end of the simulation (15 ns). However, during the period of 0.2-1.8 ns, when half of the peptide was inside the nanotube, the encapsulation was paused due to an energy barrier in the vicinity of BNNT and subsequently the external intervention, such that the self-adjustment of the peptide allowed full insertion. The free energy of the encapsulation process was -200.12 kcal·mol-1, suggesting that the insertion procedure occurred spontaneously.Once the BNNT (14,14) entered into the BNNT (20,20), the peptide was completely released after 83.8 ps. This revealed that the vdW interaction between the BNNT (14,14) and BNNT (20,20) was stronger than between BNNT (20,20) and the peptide; therefore, the BNNT (14,14) could act as a piston pushing the peptide outside the BNNT (20,20). Moreover, the sudden drop in the vdW energy between nanotubes to the value of the -1300 Kcal·mol-1 confirmed the self-insertion of the BNNT (14,14) into the BNNT (20,20) and correspondingly the release of the peptide.

    View details for DOI 10.2147/IJN.S313855

    View details for Web of Science ID 000665560400001

    View details for PubMedID 34194228

    View details for PubMedCentralID PMC8238539

  • Nanotechnology-based approaches for emerging and re-emerging viruses: Special emphasis on COVID-19. Microbial pathogenesis Saravanan, M. n., Mostafavi, E. n., Vincent, S. n., Negash, H. n., Andavar, R. n., Perumal, V. n., Chandra, N. n., Narayanasamy, S. n., Kalimuthu, K. n., Barabadi, H. n. 2021: 104908

    Abstract

    In recent decades, the major concern of emerging and re-emerging viral diseases has become an increasingly important area of public health concern, and it is of significance to anticipate future pandemic that would inevitably threaten human lives. The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a newly emerged virus that causes mild to severe pneumonia. Coronavirus disease (COVID-19) became a very much concerned issue worldwide after its super-spread across the globe and emerging viral diseases have not got specific and reliable diagnostic and treatments. As the COVID-19 pandemic brings about a massive life-loss across the globe, there is an unmet need to discover a promising and typically effective diagnosis and treatment to prevent super-spreading and mortality from being decreased or even eliminated. This study was carried out to overview nanotechnology-based diagnostic and treatment approaches for emerging and re-emerging viruses with the current treatment of the disease and shed light on nanotechnology's remarkable potential to provide more effective treatment and prevention to a special focus on recently emerged coronavirus.

    View details for DOI 10.1016/j.micpath.2021.104908

    View details for PubMedID 33932543

    View details for PubMedCentralID PMC8079947

  • In situ printing of scaffolds for reconstruction of bone defects. Acta biomaterialia Mostafavi, A. n., Abudula, T. n., Russell, C. S., Mostafavi, E. n., Williams, T. J., Salah, N. n., Alshahrie, A. n., Harris, S. n., Basri, S. M., Mishra, Y. K., Webster, T. J., Memic, A. n., Tamayol, A. n. 2021

    Abstract

    Bone defects are commonly caused by traumatic injuries and tumor removal and critically sized defects overwhelm the regenerative capacity of the native tissue. Reparative strategies such as auto, xeno, and allografts have proven to be insufficient to reconstruct and regenerate these defects. For the first time, we introduce the use of handheld melt spun three dimensional printers that can deposit materials directly within the defect site to properly fill the cavity and form free-standing scaffolds. Engineered composite filaments were generated from poly(caprolactone) (PCL) doped with zinc oxide nanoparticles and hydroxyapatite microparticles. The use of PCL-based materials allowed low-temperature printing to avoid overheating of the surrounding tissues. The in situ printed scaffolds showed moderate adhesion to wet bone tissue, which can prevent scaffold dislocation. The printed scaffolds showed to be osteoconductive and supported the osteodifferentiation of mesenchymal stem cells. Biocompatibility of the scaffolds upon in vivo printing subcutaneously in mice showed promising results.

    View details for DOI 10.1016/j.actbio.2021.03.009

    View details for PubMedID 33705990

  • Carbon Nanotubes: Smart Drug/Gene Delivery Carriers. International journal of nanomedicine Zare, H., Ahmadi, S., Ghasemi, A., Ghanbari, M., Rabiee, N., Bagherzadeh, M., Karimi, M., Webster, T. J., Hamblin, M. R., Mostafavi, E. 2021; 16: 1681–1706

    Abstract

    The unique properties of carbon nanotubes (CNTs) (such as their high surface to volume ratios, enhanced conductivity and strength, biocompatibility, ease of functionalization, optical properties, etc.) have led to their consideration to serve as novel drug and gene delivery carriers. CNTs are effectively taken up by many different cell types through several mechanisms. CNTs have acted as carriers of anticancer molecules (including docetaxel (DTX), doxorubicin (DOX), methotrexate (MTX), paclitaxel (PTX), and gemcitabine (GEM)), anti-inflammatory drugs, osteogenic dexamethasone (DEX) steroids, etc. In addition, the unique optical properties of CNTs have led to their use in a number of platforms for improved photo-therapy. Further, the easy surface functionalization of CNTs has prompted their use to deliver different genes, such as plasmid DNA (PDNA), micro-RNA (miRNA), and small interfering RNA (siRNA) as gene delivery vectors for various diseases such as cancers. However, despite all of these promises, the most important continuous concerns raised by scientists reside in CNT nanotoxicology and the environmental effects of CNTs, mostly because of their non-biodegradable state. Despite a lack of widespread FDA approval, CNTs have been studied for decades and plenty of in vivo and in vitro reports have been published, which are reviewed here. Lastly, this review covers the future research necessary for the field of CNT medicine to grow even further.

    View details for DOI 10.2147/IJN.S299448

    View details for PubMedID 33688185

  • Chitosan/PVA hydrogels incorporated with green synthesized cerium oxide nanoparticles for wound healing applications EUROPEAN POLYMER JOURNAL Kalantari, K., Mostafavi, E., Saleh, B., Soltantabar, P., Webster, T. J. 2020; 134
  • Green nanotechnology-based zinc oxide (ZnO) nanomaterials for biomedical applications: a review JOURNAL OF PHYSICS-MATERIALS Cruz, D., Mostafavi, E., Vernet-Crua, A., Barabadi, H., Shah, V., Cholula-Diaz, J. L., Guisbiers, G., Webster, T. J. 2020; 3 (3)
  • Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery BIOMATERIALS SCIENCE Zandi, N., Mostafavi, E., Shokrgozar, M., Tamjid, E., Webster, T. J., Annabi, N., Simchi, A. 2020; 8 (4): 1127–36

    Abstract

    The delivery of growth factors is often challenging due to their short half-life, low stability, and rapid deactivation. In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization. These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues. Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors. We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues. PCNs were prepared by a polymer-polymer pair reaction method and characterized for physicochemical properties. Fourier transform infrared spectroscopy (FTIR) analysis indicated that complexation occurred through electrostatic interactions. Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively. The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL. Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement. In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors. The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs). Our results demonstrated the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications.

    View details for DOI 10.1039/c9bm00668k

    View details for Web of Science ID 000517148800008

    View details for PubMedID 31389409

  • GDNF gene-engineered adipose-derived stem cells seeded Emu oil-loaded electrospun nanofibers for axonal regeneration following spinal cord injury Journal of Drug Delivery Science and Technology Nejati, K., Dadashpour, M., Ghareghomi, S., Mostafavi, E., Ebrahimi-Kalan, A., Biglari, A., Alizadeh, E., Mortazavi, Y., Zarghami, N. 2020; 60: 102095
  • Electroconductive Nanobiomaterials for Tissue Engineering and Regenerative Medicine. Bioelectricity Mostafavi, E., Medina-Cruz, D., Kalantari, K., Taymoori, A., Soltantabar, P., Webster, T. J. 2020; 2 (2): 120-149

    Abstract

    Regenerative medicine aims to engineer tissue constructs that can recapitulate the functional and structural properties of native organs. Most novel regenerative therapies are based on the recreation of a three-dimensional environment that can provide essential guidance for cell organization, survival, and function, which leads to adequate tissue growth. The primary motivation in the use of conductive nanomaterials in tissue engineering has been to develop biomimetic scaffolds to recapitulate the electrical properties of the natural extracellular matrix, something often overlooked in numerous tissue engineering materials to date. In this review article, we focus on the use of electroconductive nanobiomaterials for different biomedical applications, particularly, very recent advancements for cardiovascular, neural, bone, and muscle tissue regeneration. Moreover, this review highlights how electroconductive nanobiomaterials can facilitate cell to cell crosstalk (i.e., for cell growth, migration, proliferation, and differentiation) in different tissues. Thoughts on what the field needs for future growth are also provided.

    View details for DOI 10.1089/bioe.2020.0021

    View details for PubMedID 34471843

    View details for PubMedCentralID PMC8370325

  • Green nanomedicine: The path to the next generation of nanomaterials for diagnosing brain tumors and therapeutics? Expert opinion on drug delivery Mostafavi, E. n., Medina-Cruz, D. n., Vernet-Crua, A. n., Cheng, J. n., Cholula-Díaz, J. L., Guisbiers, G. n., Webster, T. J. 2020

    Abstract

    Introduction: Current brain cancer treatments, based on radiotherapy and chemotherapy, are sometimes successful, but they are not free of drawbacks. Areas covered: Traditional methods for the treatment of brain tumors are discussed here with new solutions presented, among which the application of nanotechnology has demonstrated promising results over the past decade. The traditional synthesis of nanostructures, which relies on the use of physicochemical methodologies are discussed, and their associated concerns in terms of environmental and health impact due to the production of toxic by-products, need for toxic catalysts, and their lack of biocompatibility are presented. An overview of the current situation of treating brain tumors using nanotechnological-based approaches is introduced, and some of the latest advances in the application of green nanomaterials (NMs) for the effective targeting of brain tumors are presented. Expert opinion: Green nanotechnology is introduced as a potential solution to toxic NMs through the application of environmentally friendly and cost-effective protocols using living organisms and biomolecules. The current status of this field, such as those involving clinical trials, is included, and the possible limitations of green-NMs and potential ways to avoid those limitations are discussed so that the field can potentially evolve.

    View details for DOI 10.1080/17425247.2021.1865306

    View details for PubMedID 33332168

  • Green Nanotechnology-based Gold Nanomaterials for Hepatic Cancer Therapeutics: A Systematic Review. Iranian journal of pharmaceutical research : IJPR Barabadi, H. n., Webster, T. J., Vahidi, H. n., Sabori, H. n., Damavandi Kamali, K. n., Jazayeri Shoushtari, F. n., Mahjoub, M. A., Rashedi, M. n., Mostafavi, E. n., Cruz, D. M., Hosseini, O. n., Saravana, M. n. 2020; 19 (3): 3–17

    Abstract

    The objective of the current study was to systematically review the in-vitro anticancer activity of green synthesized gold nanoparticles (AuNPs) against hepatic cancer cells. The articles were identified through electronic databases, including PubMed, Scopus, Embase, Web of Science, Science Direct, ProQuest, and Cochrane. In total, 20 articles were found eligible to enter into our systematic review. Our findings showed that 65% of the articles used herbal extracts for the synthesis of AuNPs. Significantly, almost all of the articles stated the biofabrication of AuNPs below 100 nm in diameter. Impressively, most of the studies showed significant anticancer activity against HepG2 cells. Molecular studies stated the induction of apoptosis through the AuNPs-treated cells. We provided valuable information about the molecular mechanisms of AuNPs-induced cytotoxicity against HepG2 cells as well as their biocompatibility. The studies represented that AuNPs can be effective as anticancer drug nanocarrier for drug delivery systems. In addition, AuNP surface functionalization provides an opportunity to design multifunctional nanoparticles by conjugating them to diagnostic and/or therapeutic agents for theranostic purposes. Overall, our findings depicted considerable biogenic AuNPs-induced cytotoxicity, however, future studies should assess the anticancer activity of biogenic AuNPs through in-vivo studies, which was missing from such studies.

    View details for DOI 10.22037/ijpr.2020.113820.14504

    View details for PubMedID 33680005

    View details for PubMedCentralID PMC7757980

  • Nanoengineered shear-thinning and bioprintable hydrogel as a versatile platform for biomedical applications. Biomaterials Zandi, N. n., Sani, E. S., Mostafavi, E. n., Ibrahim, D. M., Saleh, B. n., Shokrgozar, M. A., Tamjid, E. n., Weiss, P. S., Simchi, A. n., Annabi, N. n. 2020; 267: 120476

    Abstract

    The development of bioinks based on shear-thinning and self-healing hydrogels has recently attracted significant attention for constructing complex three-dimensional physiological microenvironments. For extrusion-based bioprinting, it is challenging to provide high structural reliability and resolution of printed structures while protecting cells from shear forces during printing. Herein, we present shear-thinning and printable hydrogels based on silicate nanomaterials, laponite (LA), and glycosaminoglycan nanoparticles (GAGNPs) for bioprinting applications. Nanocomposite hydrogels (GLgels) were rapidly formed within seconds due to the interactions between the negatively charged groups of GAGNPs and the edges of LA. The shear-thinning behavior of the hydrogel protected encapsulated cells from aggressive shear stresses during bioprinting. The bioinks could be printed straightforwardly into shape-persistent and free-standing structures with high aspect ratios. Rheological studies demonstrated fast recovery of GLgels over multiple strain cycles. In vitro studies confirmed the ability of GLgels to support cell growth, proliferation, and spreading. In vitro osteogenic differentiation of pre-osteoblasts murine bone marrow stromal cells encapsulated inside the GLgels was also demonstrated through evaluation of ALP activity and calcium deposition. The subcutaneous implantation of the GLgel in rats confirmed its in vivo biocompatibility and biodegradability. The engineered shear-thinning hydrogel with osteoinductive characteristics can be used as a new bioink for 3D printing of constructs for bone tissue engineering applications.

    View details for DOI 10.1016/j.biomaterials.2020.120476

    View details for PubMedID 33137603

  • Wound dressings functionalized with silver nanoparticles: promises and pitfalls. Nanoscale Kalantari, K. n., Mostafavi, E. n., Afifi, A. M., Izadiyan, Z. n., Jahangirian, H. n., Rafiee-Moghaddam, R. n., Webster, T. J. 2020; 12 (4): 2268–91

    Abstract

    Infections are the main reason why most people die from burns and diabetic wounds. The clinical challenge for treating wound infections through traditional antibiotics has been growing steadily and has now reached a critical status requiring a paradigm shift for improved chronic wound care. The US Centers for Disease Control have predicted more deaths from antimicrobial-resistant bacteria than from all types of cancers combined by 2050. Thus, the development of new wound dressing materials that do not rely on antibiotics is of paramount importance. Currently, incorporating nanoparticles into scaffolds represents a new concept of 'nanoparticle dressing' which has gained considerable attention for wound healing. Silver nanoparticles (Ag-NPs) have been categorized as metal-based nanoparticles and are intriguing materials for wound healing because of their excellent antimicrobial properties. Ag-NPs embedded in wound dressing polymers promote wound healing and control microorganism growth. However, there have been several recent disadvantages of using Ag-NPs to fight infections, such as bacterial resistance. This review highlights the therapeutic approaches of using wound dressings functionalized with Ag-NPs and their potential role in revolutionizing wound healing. Moreover, the physiology of the skin and wounds is discussed to place the use of Ag-NPs in wound care into perspective.

    View details for DOI 10.1039/c9nr08234d

    View details for PubMedID 31942896

  • Corrigendum to "An update on advances in new developing DNA conjugation diagnostics and ultra-resolution imaging technologies: Possible applications in medical and biotechnological utilities" [Biosens. Bioelectron Volume 144, (1 November 2019), 111633]. Biosensors & bioelectronics Mohajeri, N. n., Imani, M. n., Mostafavi, E. n., Akbarzadeh, A. n., Sadighi, A. n., Zarghamia, N. n. 2020; 151: 111904

    View details for DOI 10.1016/j.bios.2019.111904

    View details for PubMedID 31999597

  • Green nanotechnology-based drug delivery systems for osteogenic disorders. Expert opinion on drug delivery Medina-Cruz, D. n., Mostafavi, E. n., Vernet-Crua, A. n., Cheng, J. n., Shah, V. n., Cholula-Diaz, J. L., Guisbiers, G. n., Tao, J. n., García-Martín, J. M., Webster, T. J. 2020; 17 (3): 341–56

    Abstract

    Introduction: Current treatments for osteogenic disorders are often successful, however they are not free of drawbacks, such as toxicity or side effects. Nanotechnology offers a platform for drug delivery in the treatment of bone disorders, which can overcome such limitations. Nevertheless, traditional synthesis of nanomaterials presents environmental and health concerns due to its production of toxic by-products, the need for extreme and harsh raw materials, and their lack of biocompatibility over time.Areas covered: This review article contains an overview of the current status of treating osteogenic disorders employing green nanotechnological approaches, showing some of the latest advances in the application of green nanomaterials, as drug delivery carriers, for the effective treatment of osteogenic disorders.Expert opinion: Green nanotechnology, as a potential solution, is understood as the use of living organisms, biomolecules and environmentally friendly processes for the production of nanomaterials. Nanomaterials derived from bacterial cultures or biomolecules isolated from living organisms, such as carbohydrates, proteins, and nucleic acids, have been proven to be effective composites. These nanomaterials introduce enhancements in the treatment and prevention of osteogenic disorders, compared to physiochemically-synthesized nanostructures, specifically in terms of their improved cell attachment and proliferation, as well as their ability to prevent bacterial adhesion.

    View details for DOI 10.1080/17425247.2020.1727441

    View details for PubMedID 32064959

  • Enhancement of Loading Efficiency by Coloading of Doxorubicin and Quercetin in Thermoresponsive Polymeric Micelles. Biomacromolecules Soltantabar, P. n., Calubaquib, E. L., Mostafavi, E. n., Biewer, M. C., Stefan, M. C. 2020; 21 (4): 1427–36

    Abstract

    Chemotherapy faces challenges, including poor aqueous solubility of the drugs, and cardiotoxicity. Micellar drug delivery systems (DDS) are used to encapsulate anticancer drugs for better therapeutic effects, however, with poor loading content. Herein, we synthesized a micellar DDS using γ-benzyloxy substituted poly(ε-caprolactone) as the hydrophobic block and coloaded anticancer doxorubicin (Dox) and antioxidant quercetin (Que). γ-Substituted oligo(ethylene) glycol (OEG) poly(ε-caprolactone)s were used as hydrophilic blocks to make the polymers thermoresponsive. Variation of the OEG chain allowed the tunability of the lower critical solution temperature. Moreover, drug loading and release were studied. Thermodynamic stability, size, and morphology were determined by fluorescence measurements, dynamic light scattering, and transmission electron microscopy. Combination loading demonstrated improved loading of Dox and Que. Biological studies were performed using HepG2 human liver cancer and H9c2 rat heart cells. The use of biodegradable, biocompatible, and thermoresponsive polymers along with the coloading approach is a good strategy in developing DDSs.

    View details for DOI 10.1021/acs.biomac.9b01742

    View details for PubMedID 32149500

  • The feasibility and usability of DNA-dot bioconjugation to antibody for targeted in vitro cancer cell fluorescence imaging. Journal of photochemistry and photobiology. B, Biology Mohajeri, N. n., Mostafavi, E. n., Zarghami, N. n. 2020; 209: 111944

    Abstract

    DNA-protein bioconjugation is an appealing target-tracking strategy. The new capability of DNA molecule as a biological nanomaterial endows unique fluorescence and physicochemical properties to be applied in bioimaging. Progression in targeted imaging is contingent on the conjugation of diagnostic nanoparticles to biomolecular signatures, particularly antibody-based ligands. Here, we have reported our recent experience, DNA-dot synthesis and characterization, the covalent conjugation of DNA-dot to goat F(ab')2 IgG and Epidermal Growth Factor Receptor (EGFR) antibodies, DNA-dot@antibody coupling confirmation, and fluorescent targeted imaging of lung cancer cell line. As a result, the average size of DNA-dot was 4.5-5 nm which was conjugated to amine-rich antibodies with returned PO4-1 groups on the DNA-dot surface via PN bond. The synthetic DNA-dots were conjugated to the goat F(ab')2 IgG and tested for fluorescent detection usability by indirect Dot-blot assay. Also, DNA-dot@EGFR conjugates identified lung cancer cells with 84-92% specificity and 100% sensitivity in five concentrations, associated with 0.0025 to 0.04 g 100 μL-1 DNA-dot. The results demonstrated that bioconjugated DNA-dot can do the diagnosis profiling of molecular biomarkers. Generally, DNA-dot bioconjugation with antibody is implemented within two days and biomarker detection takes one day. Consequently, DNA-dot@antibody is potentially a toxic-free, swift, and efficient method of antibody labeling that opens up new horizons in fluorescent nanoimaging in the field of cancer cell detection.

    View details for DOI 10.1016/j.jphotobiol.2020.111944

    View details for PubMedID 32619869

  • Green nanotechnology-based Gold Nanomaterials for Hepatic Cancer Therapeutics: A Systematic Review Iranian Journal of Pharmaceutical Research Barabadi, H., Webster, T., Vahidi, H., Sabori, H., Kamali, K., Shoushtari, F., Mahjoub, M., Rashedi, M., Mostafavi, E., et al 2020
  • Bioactive and elastic nanocomposites with antimicrobial properties for bone tissue regeneration ACS Applied Bio Materials Ibrahim, D., Shirzaei Sani, E., Soliman, A., Zandi, N., Mostafavi, E., Youssef, A., Allam , N., Annabi, N. 2020; 3: 3313-3325

    View details for DOI 10.1021/acsabm.0c00250

  • Electroconductive Nanobiomaterials for Tissue Engineering and Regenerative Medicine Bioelectricity Mostafavi, E., et al 2020; 2 (2): 120-149

    View details for DOI 10.1089/bioe.2020.0021

  • Emerging Antineoplastic Biogenic Gold Nanomaterials for Breast Cancer Therapeutics: A Systematic Review INTERNATIONAL JOURNAL OF NANOMEDICINE Saravanan, M., Vahidi, H., Cruz, D., Vernet-Crua, A., Mostafavi, E., Stelmach, R., Webster, T. J., Mahjoub, M., Rashedi, M., Barabadi, H. 2020; 15: 3577–95

    Abstract

    Breast cancer remains as a concerning global health issue, being the second leading cause of cancer deaths among women in the United States (US) in 2019. Therefore, there is an urgent and substantial need to explore novel strategies to combat breast cancer. A potential solution may come from the use of cancer nanotechnology, an innovative field of study which investigates the potential of nanomaterials for cancer diagnosis, therapy, and theranostic applications. Consequently, the theranostic functionality of cancer nanotechnology has been gaining much attention between scientists during the past few years and is growing exponentially. The use of biosynthesized gold nanoparticles (AuNPs) has been explored as an efficient mechanism for the treatment of breast cancer. The present study supposed a global systematic review to evaluate the effectiveness of biogenic AuNPs for the treatment of breast cancer and their anticancer molecular mechanisms through in vitro studies. Online electronic databases, including Cochrane, PubMed, Scopus, Web of Science, Science Direct, ProQuest, and Embase, were searched for the articles published up to July 16, 2019. Our findings revealed that plant-mediated synthesis was the most common approach for the generation of AuNPs. Most of the studies reported spherical or nearly spherical-shaped AuNPs with a mean diameter less than 100 nm in size. A significantly larger cytotoxicity was observed when the biogenic AuNPs were tested towards breast cancer cells compared to healthy cells. Moreover, biogenic AuNPs demonstrated significant synergistic activity in combination with other anticancer drugs through in vitro studies. Although we provided strong and comprehensive preliminary in vitro data, further in vivo investigations are required to show the reliability and efficacy of these NPs in animal models.

    View details for DOI 10.2147/IJN.S240293

    View details for Web of Science ID 000533482100001

    View details for PubMedID 32547015

    View details for PubMedCentralID PMC7245458

  • Bioprinters for organs-on-chips BIOFABRICATION Miri, A. K., Mostafavi, E., Khorsandi, D., Hu, S., Malpica, M., Khademhosseini, A. 2019; 11 (4): 042002

    Abstract

    Recent advances in bioprinting technologies have enabled rapid manufacturing of organ-on-chip models along with biomimetic tissue microarchitectures. Bioprinting techniques can be used to integrate microfluidic channels and flow connections in organ-on-chip models. We review bioprinters in two categories of nozzle-based and optical-based methods, and then discuss their fabrication parameters such as resolution, replication fidelity, fabrication time, and cost for micro-tissue models and microfluidic applications. The use of bioprinters has shown successful replicates of functional engineered tissue models integrated within a desired microfluidic system, which facilitates the observation of metabolism or secretion of models and sophisticated control of a dynamic environment. This may provide a wider order of tissue engineering fabrication in mimicking physiological conditions for enhancing further applications such as drug development and pathological studies.

    View details for DOI 10.1088/1758-5090/ab2798

    View details for Web of Science ID 000487215700001

    View details for PubMedID 31170695

    View details for PubMedCentralID PMC6756175

  • Would Colloidal Gold Nanocarriers Present An Effective Diagnosis Or Treatment For Ischemic Stroke? INTERNATIONAL JOURNAL OF NANOMEDICINE Amani, H., Mostafavi, E., Alebouyeh, M., Arzaghi, H., Akbarzadeh, A., Pazoki-Toroudi, H., Webster, T. J. 2019; 14: 8013–31

    Abstract

    This study was conducted to evaluate OX26-PEG-coated gold nanoparticles (GNPs) (OX26@GNPs) as a novel targeted nanoparticulate system on cell survival after ischemic stroke.Dynamic light scattering (DLS), zeta sizer, and transmission electron microscopy (TEM) were performed to characterize the OX26@GNPs. The effect of OX26@GNPs on infarct volume, neuronal loss, and necroptosis was evaluated 24 h after reperfusion using 2, 3,5-Triphenyltetrazolium chloride (TTC) staining, Nissl staining and Western blot assay, respectively.Conjugation of OX26-PEG to the surface of the 25 nm colloidal gold particles increased their size to 32±2 nm, while a zeta potential change of -40.4 to 3.40 mV remarkably increased the stability of the nanoparticles. Most importantly, OX26@GNPs significantly increased the infarcted brain tissue, while bare GNPs and PEGylated GNPs had no effect on the infarct volume. However, our results indicated an extension of necroptotic cell death, followed by cell membrane damage.Collectively, our results showed that the presently formulated OX26@GNPs are not suitable nanocarriers nor contrast agents under oxidative stress for the diagnosis and treatment of ischemic stroke. Moreover, our findings suggest that the cytotoxicity of GNPs in the brain is significantly associated with their surface charge.

    View details for DOI 10.2147/IJN.S210035

    View details for Web of Science ID 000489021700001

    View details for PubMedID 31632015

    View details for PubMedCentralID PMC6789974

  • Three-Dimensional Graphene Foams: Synthesis, Properties, Biocompatibility, Biodegradability, and Applications in Tissue Engineering. ACS biomaterials science & engineering Amani, H. n., Mostafavi, E. n., Arzaghi, H. n., Davaran, S. n., Akbarzadeh, A. n., Akhavan, O. n., Pazoki-Toroudi, H. n., Webster, T. J. 2019; 5 (1): 193–214

    Abstract

    Presently, clinical nanomedicine and nanobiotechnology have impressively demanded the generation of new organic/inorganic analogues of graphene (as one of the intriguing biomedical research targets) for stem-cell-based tissue engineering. Among different shapes of graphene, three-dimensional (3D) graphene foams (GFs) are highly promising candidates to provide conditions for mimicking in vivo environments, affording effective cell attachment, proliferation,and differentiation due to their unique properties. These include the highest biocompatibility among nanostructures, high surface-to-volume ratio, 3D porous structure (to provide a homogeneous/isotropic growth of tissues), highly favorable mechanical characteristics, and rapid mass and electron transport kinetics (which are required for chemical/physical stimulation of differentiated cells). This review aims to describe recent and rapid advances in the fabrication of 3D GFs, together with their use in tissue engineering and regenerative nanomedicine applications. Moreover, we have summarized a broad range of recent studies about the behaviors, biocompatibility/toxicity,and biodegradability of these materials, both in vitro and in vivo. Finally, the highlights and challenges of these 3D porous structures, compared to the current polymeric scaffold competitors, are discussed.

    View details for DOI 10.1021/acsbiomaterials.8b00658

    View details for PubMedID 33405863

  • Synthesis, characterization and in vitro evaluation of magnetic nanoparticles modified with PCL-PEG-PCL for controlled delivery of 5FU (vol 46, pg S938, 2017) ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY Asadi, N., Annabi, N., Mostafavi, E., Anzabi, M., Khalilov, R., Saghfi, S., Mehrizadeh, M., Akbarzadeh, A. 2019; 47 (1): 2917
  • Nanotechnology and picotechnology: A new arena for translational medicine BIOMATERIALS IN TRANSLATIONAL MEDICINE Mostafavi, E., Soltantabar, P., Webster, T. J., Yang, L., Bhaduri, S. B., Webster, T. J. 2019: 191–212
  • The use of stromal vascular fraction (SVF), platelet-rich plasma (PRP) and stem cells in the treatment of osteoarthritis: an overview of clinical trials ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY Mehranfar, S., Rad, I., Mostafav, E., Akbarzadeh, A. 2019; 47 (1): 882–90

    Abstract

    Osteoarthritis (OA) is a major cause of disability across the world, which its prevalence is relatively high in elder population. Current accepted therapies such as exercise, anti-inflammatory drugs and intra-articular inoculation of corticosteroids are aimed at controlling symptoms in the affected patients. Surgical options including arthroplasty, osteotomy and joint replacement are other choices of treatment, which are invasive and can be applied in case of failure of conventional therapies. In the last few decades, efforts to treat musculoskeletal diseases are being increasingly focused on regenerative cellular therapies. Stromal vascular fraction (SVF), which obtained from adipose tissue, contains a variety of cells include mesenchymal stem cells (MSCs) and has shown to be effective in cartilage repair. Autologous blood products such as platelet-rich plasma (PRP) act as an adjuvant of surgical treatment and its intra-articular delivery has shown beneficial effects for OA treatment. Given the efficacy of such treatment approaches in OA, this paper discusses both preclinical and clinical evidence with major focus on clinical trials.

    View details for DOI 10.1080/21691401.2019.1576710

    View details for Web of Science ID 000461718500001

    View details for PubMedID 30887856

  • The effect of chrysin-curcumin-loaded nanofibres on the wound-healing process in male rats ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY Mohammadi, Z., Zak, M., Majdi, H., Mostafavi, E., Barati, M., Lotfimehr, H., Ghaseminasab, K., Pazoki-Toroudi, H., Webster, T. J., Akbarzadeh, A. 2019; 47 (1): 1642–52

    Abstract

    The aim of the present study was to produce chrysin-curcumin-loaded PCL-PEG nanofibres by an electrospinning technique and to evaluate the biological activity of the chrysin-curcumin-loaded PCL-PEG fibres for wound healing and its related genes using in vivo methods.The electrospinning method was carried out for the preparation of the chrysin, curcumin and chrysin-curcumin-loaded PCL-PEG nanofibres with different concentrations. FTIR and SEM were performed to characterize the chemical structures and morphology of the nanofibres. In vitro drug release, as well as in vivo wound-healing studies were investigated in male rats. The expressions of genes related to the wound-healing process were also evaluated by real-time PCR.Our study showed that the chrysin-curcumin-loaded nanofibres have anti-inflammatory properties in several stages of the wound-healing process by affecting the IL-6, MMP-2, TIMP-1, TIMP-2 and iNOS gene expression. Our results demonstrated that the effect of the chrysin-loaded nanofibre, the curcumin-loaded nanofibre and the chrysin-curcumin-loaded nanofibre in the wound-healing process is dose dependent and in accordance with the obtained results in that it might affect the inflammation phase more than the other stages of the wound-healing process.We have introduced chrysin-curcumin-loaded PCL-PEG nanofibres as a novel compound for shortening the duration of the wound-healing process.

    View details for DOI 10.1080/21691401.2019.1594855

    View details for Web of Science ID 000465907400002

    View details for PubMedID 31027431

  • Development Development and characterization of a novel conductive polyaniline-g-polystyrene/Fe(3)O(4 )nanocomposite for the treatment of cancer ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY Ahmadkhani, L., Mostafavi, E., Ghasemali, S., Baghban, R., Pazoki-Toroudi, H., Davaran, S., Malakootikhah, J., Asadi, N., Mammadova, L., Saghfi, S., Webster, T. J., Akbarzadeh, A. 2019; 47 (1): 873–81

    Abstract

    The goal of this study is to synthesize, characterize and investigate some physicochemical properties of conductive polyaniline-g-polystyrene/Fe3O4 (Fe3O4/PSt-g-PANi) nanocomposites. For this purpose, initially, Fe3O4 nanoparticles were synthesized by a co-precipitation method. Then, the desired nanocomposite was synthesized in two steps. First, the atom transfer radical polymerization (ATRP) of styrene was performed using an ATRP initiator attached to the surface of Fe3O4 nanoparticles, followed by functionalization of the Fe3O4-PSt with amine groups (-NH2). Second, surface oxidative graft copolymerization of aniline was accomplished using the -NH2 moieties on the Fe3O4/PSt-NH2 as the anchoring sites. The prepared materials were characterized by various instruments, including TEM, SEM, TGA, EDX, FT-IR, XRD and conductivity measurements. The results indicated that the synthesized conductive polymer/Fe3O4 nanocomposites had higher electrical conductivity and thermal resistance than those of the corresponding homopolymers.

    View details for DOI 10.1080/21691401.2019.1575839

    View details for Web of Science ID 000461286000003

    View details for PubMedID 30873875

  • Three-Dimensional Graphene Foams: Synthesis, Properties, Biocompatibility, Biodegradability, and Applications in Tissue Engineering ACS BIOMATERIALS SCIENCE & ENGINEERING Amani, H., Mostafavi, E., Arzaghi, H., Davaran, S., Akbarzadeh, A., Akhavan, O., Pazoki-Toroudi, H., Webster, T. J. 2019; 5 (1): 193–214
  • Fabrication of Three-Dimensional Scaffolds Based on Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering ACS OMEGA Del Bakhshayesh, A., Mostafavi, E., Alizadeh, E., Asadi, N., Akbarzadeh, A., Davaran, S. 2018; 3 (8): 8605–11

    Abstract

    Three-dimensional (3D) biodegradable and biomimetic porous scaffolds are ideal frameworks for skin tissue engineering. In this study, hybrid constructs of 3D scaffolds were successfully fabricated by the freeze-drying method from combinations of the type I collagen (Col) and synthetic poly(lactic acid) (PLLA) or polycaprolactone (PCL). Four different groups of 3D porous scaffolds including PCL, PCL-Col, PCL-PLLA, and PCL-PLLA-Col were fabricated and systematically characterized by hydrogen nuclear magnetic resonance, Fourier transform infrared spectroscopy, and scanning electron microscopy (SEM). Adipose tissue-derived mesenchymal stem cells (AT-MSCs) were seeded in all scaffolds, and the viability, proliferation, and adhesion of the cells were investigated using dimethylthiazol diphenyltetrazolium bromide assay and SEM. The results showed that scaffolds containing Col, particularly PCL-PLLA-Col scaffold, with pore sizes close to 400 nm and being sufficiently interconnected, have significantly greater potential (p < 0.01) for encouraging AT-MSCs adhesion and growth. The PCL-PLLA provided a mechanically stronger mesh support, and the type I Col microsponges encouraged excellent cell adhesion and tissue formation. The scaffold with the best properties could be an appropriate functional candidate for the preparation of artificial skin constructs.

    View details for DOI 10.1021/acsomega.8b01219

    View details for Web of Science ID 000440617900018

    View details for PubMedID 31458990

    View details for PubMedCentralID PMC6644454

  • Role of dendrimers in advanced drug delivery and biomedical applications: A Review Experimental Oncology Akbarzadeh, A., Khalilov, R., Mostafavi, E., et al 2018; 40 (3): 1-6
  • Current developments in green synthesis of metallic nanoparticles using plant extracts: a review ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY Yadi, M., Mostafavi, E., Saleh, B., Davaran, S., Aliyeva, I., Khalilov, R., Nikzamir, M., Nikzamir, N., Akbarzadeh, A., Panahi, Y., Milani, M. 2018; 46: S336–S343

    Abstract

    Metal nanoparticles (MNPs) produced by green approaches have received global attention because of their physicochemical characteristics and their applications in the field of biotechnology. In recent years, the development of synthesizing NPs by plant extracts has become a major focus of researchers because of these NPs have low hazardous effect in the environment and low toxicity for the human body. Synthesized NPs from plants are not only more stable in terms of size and shape, also the yield of this method is higher than the other methods. Moreover, some of these MNPs have shown antimicrobial activity which is consistently confirmed in past few years. Plant extracts have been used as reducing agent and stabilizer of NPs in which we can reduce the toxicity in the environment as well as the human body only by not using chemical agents. Furthermore, the presence of some specific materials in plant extracts could be extremely helpful and effective for the human body; for instance, polyphenol, which may have antioxidant effects has the capability for capturing free radicals before they can react with other biomolecules and cause serious damages. In this article, we focused on of the most common plants which are regularly used to synthesize MNPs along with various methods for synthesizing MNPs from plant extracts.

    View details for DOI 10.1080/21691401.2018.1492931

    View details for Web of Science ID 000460141900033

    View details for PubMedID 30043657

  • Synthesis, characterization and in vitro evaluation of magnetic nanoparticles modified with PCL-PEG-PCL for controlled delivery of 5FU ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY Asadi, N., Annabi, N., Mostafavi, E., Anzabi, M., Khalilov, R., Saghfi, S., Mehrizadeh, M., Akbarzadeh, A. 2018; 46: S938–S945

    Abstract

    Magnetic nanoparticles have properties that cause to apply them in cancer therapy and vehicles for the delivery of drugs such as 5FU, especially when they are modified with biocompatible copolymers. The aim of this study is to modify superparamagnetic iron oxide nanoparticles (SPIONPs) with PCL-PEG-PCL copolymers and then utilization of these nanoparticles for encapsulation of anticancer drug 5FU. The ring-opening polymerization (ROP) was used for the synthesis of PCL-PEG-PCL copolymer by ε-caprolactone (PCL) and polyethylene glycol (PEG2000). We used the double emulsion method (water/oil/water) to prepare 5FU-encapsulated Fe3O4 magnetic nanoparticles modified with PCL-PEG-PCL copolymer. Chemical structure and magnetic properties of 5FU-loaded magnetic-polymer nanoparticles were investigated systematically by employing FT-IR, XRD, VSM and SEM techniques. In vitro release profile of 5FU-loaded NPs was also determined. The results showed that the encapsulation efficiency value for nanoparticles were 90%. Moreover, the release of 5FU is significantly higher at pH 5.8 compared to pH 7.4. Therefore, these nanoparticles have sustained release and can apply for cancer therapy.

    View details for DOI 10.1080/21691401.2018.1439839

    View details for Web of Science ID 000457049400087

    View details for PubMedID 29468888

  • Nanostructured Fibrous Membranes with Rose Spike-Like Architecture NANO LETTERS Nasajpour, A., Mandla, S., Shree, S., Mostafavi, E., Sharifi, R., Khalilpour, A., Saghazadeh, S., Hassan, S., Mitchell, M. J., Leijten, J., Hou, X., Moshaverinia, A., Annabi, N., Adelung, R., Mishra, Y., Shin, S., Tamayol, A., Khademhosseini, A. 2017; 17 (10): 6235–40

    Abstract

    Nanoparticles have been used for engineering composite materials to improve the intrinsic properties and/or add functionalities to pristine polymers. The majority of the studies have focused on the incorporation of spherical nanoparticles within the composite fibers. Herein, we incorporate anisotropic branched-shaped zinc oxide (ZnO) nanoparticles into fibrous scaffolds fabricated by electrospinning. The addition of the branched particles resulted in their protrusion from fibers, mimicking the architecture of a rose stem. We demonstrated that the encapsulation of different-shape particles significantly influences the physicochemical and biological activities of the resultant composite scaffolds. In particular, the branched nanoparticles induced heterogeneous crystallization of the polymeric matrix and enhance the ultimate mechanical strain and strength. Moreover, the three-dimensional (3D) nature of the branched ZnO nanoparticles enhanced adhesion properties of the composite scaffolds to the tissues. In addition, the rose stem-like constructs offered excellent antibacterial activity, while supporting the growth of eukaryote cells.

    View details for DOI 10.1021/acs.nanolett.7b02929

    View details for Web of Science ID 000413057500052

    View details for PubMedID 28819978

    View details for PubMedCentralID PMC5683165

  • Nanostructured MnCo2O4 synthesized via co-precipitation method for SOFC interconnect application INTERNATIONAL JOURNAL OF HYDROGEN ENERGY Lesani, P., Babaei, A., Ataie, A., Mostafavi, E. 2016; 41 (45): 20640–49
  • Fabrication and characterization of nanostructured Ba-doped BiFeO3 porous ceramics MATERIALS SCIENCE-POLAND Mostafavi, E., Ataie, A. 2016; 34 (1): 148–56
  • High performance Ni-CNTs catalyst: synthesis and characterization RSC ADVANCES Gharegozloo, S., Ataie, A., Abdizadeh, H., Mostafavi, E., Parnian, M. J., Khodadadi, A. A. 2016; 6 (52): 47072–82

    View details for DOI 10.1039/c6ra03052a

    View details for Web of Science ID 000377254800111

  • Intermediate milling energy optimization to enhance the characteristics of barium hexaferrite magnetic nanoparticles JOURNAL OF ALLOYS AND COMPOUNDS Hodaei, A., Ataie, A., Mostafavi, E. 2015; 640: 162–68
  • Destructive Interactions between Pore Forming Agents and Matrix Phase during the Fabrication Process of Porous BiFeO3 Ceramics JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY Mostafavi, E., Ataie, A. 2015; 31 (8): 798–805
  • Synthesis of nano-structured Bi1-xBaxFeO3 ceramics with enhanced magnetic and electrical properties MATERIALS CHEMISTRY AND PHYSICS Mostafavi, E., Ataie, A., Ahmadzadeh, M., Palizdar, M., Comyn, T. P., Bell, A. J. 2015; 162: 106–12
  • The effects of mechanical activation energy on the solid-state synthesis process of BiFeO3 JOURNAL OF ALLOYS AND COMPOUNDS Ahmadzadeh, M., Ataie, A., Mostafavi, E. 2015; 622: 548–56
  • Synthesis of nano-structured La0.6Sr0.4Co0.2Fe0.8O3 perovskite by co-precipitation method Journal of Ultrafine Grained and Nanostructured Materials Mostafavi, E., Babaei, A., Ataie, A. 2015; 48: 45-52
  • Characterization of nano-structured multiferroic bismuth ferrite produced via solid state reaction route Mostafavi, E., Ataie, A., Ahmadzadeh, M., Parsa, M. H. TRANS TECH PUBLICATIONS LTD. 2014: 683–87
  • Synthesis of nano-structured bismuth ferrite by mechano-thermal route Ahmadzadeh, M., Ataie, A., Mostafavi, E., Parsa, M. H. TRANS TECH PUBLICATIONS LTD. 2014: 722–26
  • La0.6Sr0.4Co0.2Fe0.8O3 perovskite cathode for Intermediate temperature Solid Oxide Fuel Cell: A comparative study Iranian Journal of Hydrogen & Fuel Cells Mostafavi, E. 2014; 4: 239-246

    View details for DOI 10.22104/IJHFC.2015.175