Honors & Awards


  • Richard Armstrong Prize for Research Excellence in Chemical Biology, Vanderbilt University (August 2022)
  • Department of Chemistry and Biochemistry Leadership Award, University of Notre Dame (May 2018)
  • Outstanding Chemistry Forum Presentation, Vanderbilt University (May 2021)
  • Outstanding Independent Research Proposal, Vanderbilt University (May 2022)

Professional Education


  • Ph.D., Vanderbilt University, Chemistry (2022)
  • B.S., University of Notre Dame, Chemistry, Philosophy (2018)

Stanford Advisors


All Publications


  • Rapid Multivariate Analysis Approach to Explore Differential Spatial Protein Profiles in Tissue JOURNAL OF PROTEOME RESEARCH Sharman, K., Patterson, N., Weiss, A., Neumann, E. K., Guiberson, E. R., Ryan, D. J., Gutierrez, D. B., Spraggins, J. M., Van de Plas, R., Skaar, E. P., Caprioli, R. M. 2022

    Abstract

    Spatially targeted proteomics analyzes the proteome of specific cell types and functional regions within tissue. While spatial context is often essential to understanding biological processes, interpreting sub-region-specific protein profiles can pose a challenge due to the high-dimensional nature of the data. Here, we develop a multivariate approach for rapid exploration of differential protein profiles acquired from distinct tissue regions and apply it to analyze a published spatially targeted proteomics data set collected from Staphylococcus aureus-infected murine kidney, 4 and 10 days postinfection. The data analysis process rapidly filters high-dimensional proteomic data to reveal relevant differentiating species among hundreds to thousands of measured molecules. We employ principal component analysis (PCA) for dimensionality reduction of protein profiles measured by microliquid extraction surface analysis mass spectrometry. Subsequently, k-means clustering of the PCA-processed data groups samples by chemical similarity. Cluster center interpretation revealed a subset of proteins that differentiate between spatial regions of infection over two time points. These proteins appear involved in tricarboxylic acid metabolomic pathways, calcium-dependent processes, and cytoskeletal organization. Gene ontology analysis further uncovered relationships to tissue damage/repair and calcium-related defense mechanisms. Applying our analysis in infectious disease highlighted differential proteomic changes across abscess regions over time, reflecting the dynamic nature of host-pathogen interactions.

    View details for DOI 10.1021/acs.jproteome.2c00206

    View details for Web of Science ID 000830838200001

    View details for PubMedID 35849531

  • Multimodal Imaging Mass Spectrometry of Murine Gastrointestinal Tract with Retained Luminal Content JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY Guiberson, E. R., Good, C. J., Wexler, A. G., Skaar, E. P., Caprioli, R. M., Spraggins, J. M. 2022; 33 (6): 1073-1076

    Abstract

    The gastrointestinal tract, including luminal content, harbors a complex mixture of microorganisms, host dietary content, and immune factors. Existing imaging approaches remove luminal content and only visualize small regions of the GI tract. Here, we demonstrate a workflow for multimodal imaging using matrix-assisted laser desorption/ionization imaging mass spectrometry, autofluorescence, and bright field microscopy for mapping intestinal tissue and luminal content. Results comparing tissue and luminal content in control murine tissue show both unique molecular and elemental distributions and abundances using multimodal protein, lipid, and elemental imaging. For instance, lipid PC(42:1) is 2× higher intensity in luminal content than tissue, while PC(32:0) is 80× higher intensity in tissue. Additionally, some ions such as the protein at m/z 3443 and the element manganese are only detected in luminal content, while the protein at m/z 8564 was only detected in tissue and phosphorus had 2× higher abundance in tissue. These data highlight the robust molecular information that can be gained from the gastrointestinal tract with the inclusion of luminal content.

    View details for DOI 10.1021/jasms.1c00360

    View details for Web of Science ID 000807481600019

    View details for PubMedID 35545232

    View details for PubMedCentralID PMC9264265

  • Clostridioides difficile infection induces a rapid influx of bile acids into the gut during colonization of the host CELL REPORTS Wexler, A. G., Guiberson, E. R., Beavers, W. N., Shupe, J. A., Washington, M., Lacy, D., Caprioli, R. M., Spraggins, J. M., Skaar, E. P. 2021; 36 (10): 109683

    Abstract

    Clostridioides difficile is the leading cause of nosocomial intestinal infections in the United States. Ingested C. difficile spores encounter host bile acids and other cues that are necessary for germinating into toxin-producing vegetative cells. While gut microbiota disruption (often by antibiotics) is a prerequisite for C. difficile infection (CDI), the mechanisms C. difficile employs for colonization remain unclear. Here, we pioneered the application of imaging mass spectrometry to study how enteric infection changes gut metabolites. We find that CDI induces an influx of bile acids into the gut within 24 h of the host ingesting spores. In response, the host reduces bile acid biosynthesis gene expression. These bile acids drive C. difficile outgrowth, as mice receiving the bile acid sequestrant cholestyramine display delayed colonization and reduced germination. Our findings indicate that C. difficile may facilitate germination upon infection and suggest that altering flux through bile acid pathways can modulate C. difficile outgrowth in CDI-prone patients.

    View details for DOI 10.1016/j.celrep.2021.109683

    View details for Web of Science ID 000693615400026

    View details for PubMedID 34496241

    View details for PubMedCentralID PMC8445666

  • Spatially Targeted Proteomics of the Host-Pathogen Interface during Staphylococcal Abscess Formation ACS INFECTIOUS DISEASES Guiberson, E. R., Weiss, A., Ryan, D. J., Monteith, A. J., Sharman, K., Gutierrez, D. B., Perry, W. J., Caprioli, R. M., Skaar, E. P., Spraggins, J. M. 2021; 7 (1): 101-113

    Abstract

    Staphylococcus aureus is a common cause of invasive and life-threatening infections that are often multidrug resistant. To develop novel treatment approaches, a detailed understanding of the complex host-pathogen interactions during infection is essential. This is particularly true for the molecular processes that govern the formation of tissue abscesses, as these heterogeneous structures are important contributors to staphylococcal pathogenicity. To fully characterize the developmental process leading to mature abscesses, temporal and spatial analytical approaches are required. Spatially targeted proteomic technologies such as micro-liquid extraction surface analysis offer insight into complex biological systems including detection of bacterial proteins and their abundance in the host environment. By analyzing the proteomic constituents of different abscess regions across the course of infection, we defined the immune response and bacterial contribution to abscess development through spatial and temporal proteomic assessment. The information gathered was mapped to biochemical pathways to characterize the metabolic processes and immune strategies employed by the host. These data provide insights into the physiological state of bacteria within abscesses and elucidate pathogenic processes at the host-pathogen interface.

    View details for DOI 10.1021/acsinfecdis.0c00647

    View details for Web of Science ID 000609249400009

    View details for PubMedID 33270421

    View details for PubMedCentralID PMC7796985