All Publications

  • Hypotympanic Sound Baffle for Amelioration of Pulsatile Tinnitus due to Carotid and Jugular Bulb Dehiscence. Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology Liu, G. S., Blevins, N. H., Vaisbuch, Y. 2019; 40 (7): 920–26


    To share our experience with treating pulsatile tinnitus by insulating a dehiscent carotid artery with a hypotympanic sound baffle, and compare outcomes with a similar resurfacing approach for jugular bulb wall anomalies.Retrospective case series.Tertiary academic medical center.Adult patients with troublesome pulsatile tinnitus with radiologic evidence of carotid artery dehiscence or jugular bulb wall anomaly within the temporal bone.Hypotympanic exposure of vessel followed by resurfacing using hydroxyapatite cement (carotid dehiscence) or autologous tissue (jugular bulb wall anomalies).Alleviation or reduction of pulsatile tinnitus.Two patients presented with unilateral, debilitating pulsatile tinnitus and history and imaging consistent with carotid dehiscence and underwent hypotympanic resurfacing with hydroxyapatite cement. Both had considerable initial improvement of tinnitus, and 40% resolution of tinnitus with improved quality of life at an average follow-up of 13.5 months. Two patients with jugular bulb dehiscence/diverticulum treated by resurfacing had complete elimination of symptoms at an average follow up of 17.3 months. There were no major adverse outcomes (permanent hearing loss, vascular injury, or intracranial hypertension).Creation of a hypotympanic sound baffle offers promise as a means of reducing pulsatile tinnitus emanating from a dehiscent carotid artery transmitted to the tympanum, with substantial improvement in reported functional ability. Treatment of venous etiologies of pulsatile tinnitus with similar techniques demonstrates higher success rates, which may be attributable to incomplete resurfacing of carotid artery dehiscence along its extent towards the petrous apex due to safety concerns.

    View details for DOI 10.1097/MAO.0000000000002293

    View details for PubMedID 31295200

  • Systematic Review of Temporal Bone-Resurfacing Techniques for Pulsatile Tinnitus Associated with Vascular Wall Anomalies. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery Liu, G. S., Boursiquot, B. C., Blevins, N. H., Vaisbuch, Y. 2019: 194599818823205


    To systematically review literature evidence on temporal bone-resurfacing techniques for pulsatile tinnitus (PT) associated with vascular wall anomalies.We searched PubMed, Embase, and the Cochrane Database. The period covered was from 1962 to 2018.We included studies in all languages that reported resurfacing outcomes for patients with PT and radiographic evidence or direct visualization of sigmoid sinus wall anomaly, jugular bulb wall anomaly, or dehiscent or aberrant internal carotid artery.Of 954 citations retrieved in database searches and 5 citations retrieved from reference lists, 20 studies with a total of 141 resurfacing cases involving 138 patients were included. Resurfacing outcomes for arterial sources of PT showed 3 of 5 cases (60%) with complete resolution and 2 (40%) with partial resolution. Jugular bulb sources of PT showed 11 of 14 cases (79%) with complete resolution and 1 (7%) with partial resolution. Sigmoid sinus sources of PT showed 91 of 121 cases (75%) with complete resolution and 12 (10%) with partial resolution. Symptoms occurred more in females and on the right side. Most cases (94%) used hard-density materials for resurfacing. Material density did not appear to be associated with resurfacing outcomes. Use of autologous materials was associated with improved outcomes for arterial sources resurfacing. Major complications involving sigmoid sinus thrombosis or compression were reported in 4% of cases without long-term morbidity or mortality.Resurfacing surgery is likely effective and well tolerated for select patients with PT associated with various vascular wall anomalies.

    View details for PubMedID 30667295

  • Thyroid cancer risk in airline cockpit and cabin crew: a meta-analysis. Cancers of the head & neck Liu, G. S., Cook, A., Richardson, M., Vail, D., Holsinger, F. C., Oakley-Girvan, I. 2018; 3: 7


    Airline crew are exposed to ionizing radiation as part of their occupation and have a documented increased risk of melanoma and cataracts. However, whether their occupation predisposes them to an increased risk of thyroid cancer is not established. The purpose of this systematic review and meta-analysis was to assess the risk of thyroid cancer in airline cockpit and cabin crew compared with the general population.The MEDLINE database accessed via PubMed and Cochrane Database were searched. We included cohort studies reporting the standardized incidence ratio (SIR) or standardized mortality ratio (SMR) of thyroid cancers in any flight-based occupation.Of the 1777 citations retrieved in PubMed, eight studies with a total of 243,088 aircrew members and over 3,334,114 person-years of follow-up were included in this meta-analysis. No relevant studies were identified on Cochrane Database. The overall summary SIR of participants in any flight-based occupation was 1.11 (95% CI, 0.79-1.57; p = 0.613; 6 records). The summary SIR for cockpit crew was 1.21 (95% CI, 0.75-1.95; p = 0.383; 4 records) and the summary SIR for cabin crew was 1.00 (95% CI, 0.60-1.66; p = 0.646; 2 records). The overall summary standardized mortality ratio for airline crew was 1.19 (95% CI, 0.59-2.39; p = 0.773; 2 records).Airline crew were not found to have a significantly elevated risk of thyroid cancer incidence or mortality relative to the general population. Future research should capitalize on the growing occupational cohort dataset and employ innovative methods to quantify lifetime radiation exposure to further assess thyroid cancer risk in airline crew.

    View details for PubMedID 31093360

  • The effect of antibiotics on protein diffusion in the Escherichia coli cytoplasmic membrane PLOS ONE Liu, G. S., Bratton, B. P., Gital, Z., Shaevitz, J. W. 2017; 12 (10): e0185810


    Accumulating evidence suggests that molecular motors contribute to the apparent diffusion of molecules in cells. However, current literature lacks evidence for an active process that drives diffusive-like motion in the bacterial membrane. One possible mechanism is cell wall synthesis, which involves the movement of protein complexes in the cell membrane circumferentially around the cell envelope and may generate currents in the lipid bilayer that advectively transport other transmembrane proteins. We test this hypothesis in Escherichia coli using drug treatments that slow cell wall synthesis and measure their effect on the diffusion of the transmembrane protein mannitol permease using fluorescence recovery after photobleaching. We found no clear decrease in diffusion in response to vancomycin and no decrease in response to mecillinam treatment. These results suggest that cell wall synthesis is not an active contributor to mobility in the cytoplasmic membrane.

    View details for DOI 10.1371/journal.pone.0185810

    View details for Web of Science ID 000412163100047

    View details for PubMedID 28977034

    View details for PubMedCentralID PMC5627921

  • ELHnet: a convolutional neural network for classifying cochlear endolymphatic hydrops imaged with optical coherence tomography BIOMEDICAL OPTICS EXPRESS Liu, G. S., Zhu, M. H., Kim, J., Raphael, P., Applegate, B. E., Oghalai, J. S. 2017; 8 (10): 4579–94


    Detection of endolymphatic hydrops is important for diagnosing Meniere's disease, and can be performed non-invasively using optical coherence tomography (OCT) in animal models as well as potentially in the clinic. Here, we developed ELHnet, a convolutional neural network to classify endolymphatic hydrops in a mouse model using learned features from OCT images of mice cochleae. We trained ELHnet on 2159 training and validation images from 17 mice, using only the image pixels and observer-determined labels of endolymphatic hydrops as the inputs. We tested ELHnet on 37 images from 37 mice that were previously not used, and found that the neural network correctly classified 34 of the 37 mice. This demonstrates an improvement in performance from previous work on computer-aided classification of endolymphatic hydrops. To the best of our knowledge, this is the first deep CNN designed for endolymphatic hydrops classification.

    View details for PubMedID 29082086

  • Computer-aided detection and quantification of endolymphatic hydrops within the mouse cochlea in vivo using optical coherence tomography JOURNAL OF BIOMEDICAL OPTICS Liu, G. S., Kim, J., Applegate, B. E., Oghalai, J. S. 2017; 22 (7): 76002


    Diseases that cause hearing loss and/or vertigo in humans such as Meniere’s disease are often studied using animal models. The volume of endolymph within the inner ear varies with these diseases. Here, we used a mouse model of increased endolymph volume, endolymphatic hydrops, to develop a computer-aided objective approach to measure endolymph volume from images collected

    View details for PubMedID 28687821

  • Explaining the Coincidence Rule for Estimating Respiratory Compensation in Metabolic Acid-Base Disorders ANNALS OF INTERNAL MEDICINE Liu, G. S., Bhalla, V. 2017; 166 (8): 610-610

    View details for DOI 10.7326/L16-0470

    View details for Web of Science ID 000399304000033

    View details for PubMedID 28384697

  • Superresolution microscope image reconstruction by spatiotemporal object decomposition and association: application in resolving t-tubule structure in skeletal muscle OPTICS EXPRESS Sun, M., Huang, J., Bunyak, F., Gumpper, K., De, G., Sermersheim, M., Liu, G., Lin, P., Palaniappan, K., Ma, J. 2014; 22 (10): 12160-12176


    One key factor that limits resolution of single-molecule superresolution microscopy relates to the localization accuracy of the activated emitters, which is usually deteriorated by two factors. One originates from the background noise due to out-of-focus signals, sample auto-fluorescence, and camera acquisition noise; and the other is due to the low photon count of emitters at a single frame. With fast acquisition rate, the activated emitters can last multiple frames before they transiently switch off or permanently bleach. Effectively incorporating the temporal information of these emitters is critical to improve the spatial resolution. However, majority of the existing reconstruction algorithms locate the emitters frame by frame, discarding or underusing the temporal information. Here we present a new image reconstruction algorithm based on tracklets, short trajectories of the same objects. We improve the localization accuracy by associating the same emitters from multiple frames to form tracklets and by aggregating signals to enhance the signal to noise ratio. We also introduce a weighted mean-shift algorithm (WMS) to automatically detect the number of modes (emitters) in overlapping regions of tracklets so that not only well-separated single emitters but also individual emitters within multi-emitter groups can be identified and tracked. In combination with a maximum likelihood estimator method (MLE), we are able to resolve low to medium density of overlapping emitters with improved localization accuracy. We evaluate the performance of our method with both synthetic and experimental data, and show that the tracklet-based reconstruction is superior in localization accuracy, particularly for weak signals embedded in a strong background. Using this method, for the first time, we resolve the transverse tubule structure of the mammalian skeletal muscle.

    View details for DOI 10.1364/OE.22.012160

    View details for Web of Science ID 000336957700078

    View details for PubMedID 24921337

    View details for PubMedCentralID PMC4162352

  • IS THE BEDSIDE TIMED VIBRATION TEST RELIABLE? MUSCLE & NERVE Botez, S. A., Liu, G., Logigian, E., Herrmann, D. N. 2009; 39 (2): 221-223


    The timed vibration test (TVT) is an easy-to-perform bedside sensory test; however, its reliability is not well established at sites commonly used for clinical testing. We evaluated intra- and interrater reliability of the TVT in a healthy control cohort of 25 adult volunteers and assessed the influence of neurologic training on TVT. Intrarater [intraclass correlation coefficient (ICC) range 0.79-0.92] and interrater (ICC range 0.0.82-0.95) reliability of TVT was high at different sites, which suggests that TVT is a reliable bedside examination when performed using a standardized protocol.

    View details for DOI 10.1002/mus.21143

    View details for Web of Science ID 000262837700012

    View details for PubMedID 19145659