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  • Metformin suppresses calcium oxalate crystal-induced kidney injury by promoting Sirt1 and M2 macrophage-mediated anti-inflammatory activation. Signal transduction and targeted therapy Liu, H., Duan, C., Yang, X., Liu, J., Deng, Y., Tiselius, H., Ye, Z., Wang, T., Xing, J., Xu, H. 2023; 8 (1): 38

    View details for DOI 10.1038/s41392-022-01232-3

    View details for PubMedID 36702833

  • The influencing factors of infectious complications after percutaneous nephrolithotomy: a systematic review and meta-analysis. Urolithiasis Zhou, G., Zhou, Y., Chen, R., Wang, D., Zhou, S., Zhong, J., Zhao, Y., Wan, C., Yang, B., Xu, J., Geng, E., Li, G., Huang, Y., Liu, H., Liu, J. 2022; 51 (1): 17

    Abstract

    Infection is the most common complications of percutaneous nephrolithotomy (PCNL) in treating urinary calculi. However, the risk factors for developing infectious complications after surgery have not been clarified, and the predictive value of some factors is controversial. This study aimed to assess the risk factors for postoperative infectious complications of PCNL. We performed a systematic search of PubMed, Web of Science, Cochrane Library, and EMBASE to obtain studies reporting risk factors for postoperative infection complications after PCNL. In this review, demographic factors, laboratory test factors, and perioperative factors were evaluated. The odds ratio (OR) or mean difference (MD) with a 95% confidence interval (CI) was calculated to assess the risk factors. A total of 18 studies were included, with a total of 7161 study patients with a mean age of 46.4 to 55.5years and an incidence of infectious complications after PCNL ranging from 2.4% to 40.4%. Twelve factors were identified as independent risk factors for post-PCNL infection complications (P<0.05), female (OR=1.60, 95% CI 1.23-2.07), positive urine culture (UC) (OR=3.16, 95% CI 2.11-4.74), positive renal pelvis urine culture (RPUC) (OR=5.81, 95% CI 1.75-19.32), positive stone culture (SC) (OR=5.11, 95% CI 1.46-17.89), positive urine leukocyte (OR=3.61, 95% CI 2.45-5.34), infected stones (OR=7.00, 95% CI 1.27-38.55), elevated blood leukocyte (MD=0.71, 95% CI 0.31-1.10), elevated neutrophil-to-lymphocyte ratio (NLR) (MD=0.55, 95% CI 0.43-0.66), preoperative stenting (OR=1.55, 95% CI 1.10-2.20), multiple puncture access (OR=2.58, 95% CI 1.75-3.82), prolonged operative time (MD=10 20, 95% CI 4.80-15.60), and postoperative residual stone (OR=1.56, 95% CI 1.24-1.98). Female, UC positivity, RPUC positivity, SC positivity, urine leukocyte positivity, infected stones, elevated peripheral blood leukocytes, elevated NLR, preoperative stent implantation, multiple puncture channels, prolonged operation time, and postoperative residual stones were identified as independent risk factors for infection complications after PCNL.

    View details for DOI 10.1007/s00240-022-01376-5

    View details for PubMedID 36515726

  • Identification of Resolvin D1 and Protectin D1 as Potential Therapeutic Agents for Treating Kidney Stones. Oxidative medicine and cellular longevity Wang, B., Wei, J., Huangfu, Q., Gao, F., Qin, L., Zhong, J., Wen, J., Ye, Z., Yang, X., Liu, H. 2022; 2022: 4345037

    Abstract

    Intrarenal calcium oxalate (CaOx) crystals induce renal tubular epithelial cell (TEC) inflammatory and oxidative injury. This study is aimed at exploring potential therapeutic lipid components in kidney stones because lipids are involved in the development of several diseases and indicate the risk of kidney stones. Serum specimens were collected from 35 kidney stone patients and 35 normal controls. The lipid components in serum were measured, and differences were analyzed. The documented biological importance was comprehensively reviewed to identify lipids that differed significantly between the two groups to find potential agents associated with kidney stones. CaOx nephrocalcinosis mouse model was established to examine the therapeutic effects of specific lipids on CaOx deposition and CaOx-induced oxidative renal injury. Several lipids with significantly different levels were present in the serum of patients with stones and normal controls. Resolvin D1 (RvD1) (4.93-fold change, P < 0.001) and protectin D1 (PD1) (5.06-fold change, P < 0.001) were significantly decreased in the serum of patients with kidney stones, and an integrative review suggested that these factors might be associated with inflammatory responses, which is a crucial mechanism associated with stone damage. The administration of RvD1 and PD1 significantly inhibited kidney CaOx deposition and suppressed CaOx-induced renal tubular cell inflammatory injury and necrosis in a CaOx nephrocalcinosis mouse model. Furthermore, RvD1 and PD1 facilitated the expression of the oxidative indicator superoxide dismutase 2 (SOD2), inhibited NADPH oxidase 2 (NOX2) expression, and diminished intracellular reactive oxygen species (ROS) levels. This study preliminarily elucidated the role of lipids in kidney stones. The inhibitory effects of RvD1 and PD1 on oxidative damage induced by CaOx deposition provide a promising perspective for kidney stone treatment strategies.

    View details for DOI 10.1155/2022/4345037

    View details for PubMedID 35251472

  • Comprehensive characteristics of pathological subtypes in testicular germ cell tumor: Gene expression, mutation and alternative splicing. Frontiers in immunology Yao, X., Zhou, H., Duan, C., Wu, X., Li, B., Liu, H., Zhang, Y. 2022; 13: 1096494

    Abstract

    Background: Testicular germ cell tumor (TGCT) is the most common tumor in young men, but molecular signatures, especially the alternative splicing (AS) between its subtypes have not yet been explored.Methods: To investigate the differences between TGCT subtypes, we comprehensively analyzed the data of gene expression, alternative splicing (AS), and somatic mutation in TGCT patients from the TCGA database. The gene ontology (GO) enrichment analyses were used to explore the function of differentially expressed genes and spliced genes respectively, and Spearman correlation analysis was performed to explore the correlation between differential genes and AS events. In addition, the possible patterns in which AS regulates gene expression were elaborated by the ensemble database transcript atlas. And, we identified important transcription factors that regulate gene expression and AS and functionally validated them in TGCT cell lines.Results: We found significant differences between expression and AS in embryonal carcinoma and seminoma, while mixed cell tumors were in between. GO enrichment analyses revealed that both differentially expressed and spliced genes were enriched in transcriptional regulatory pathways, and obvious correlation between expression and AS events was determined. By analyzing the transcript map and the sites where splicing occurs, we have demonstrated that AS regulates gene expression in a variety of ways. We further identified two pivot AS-related molecules (SOX2 and HDAC9) involved in AS regulation, which were validated in embryonal carcinoma and seminoma cell lines. Differences in somatic mutations between subtypes are also of concern, with our results suggesting that mutations in some genes (B3GNT8, CAPN7, FAT4, GRK1, TACC2, and TRAM1L1) occur only in embryonal carcinoma, while mutations in KIT, KARS, and NRAS are observed only in seminoma.Conclusions: In conclusion, our analysis revealed the differences in gene expression, AS and somatic mutation among TGCT subtypes, providing a molecular basis for clinical diagnosis and precise therapy of TGCT patients.

    View details for DOI 10.3389/fimmu.2022.1096494

    View details for PubMedID 36713456