Idan Steinberg received his B.Sc. (2008, with honors), M.Sc. (2011, with honors), and Ph.D. (2016) degrees in Biomedical Engineering from Tel Aviv University.
He is currently a postdoctoral researcher in the Multimodality Molecular Imaging Lab (MMIL) of Dr. Sam Gambhir at Stanford University.
His M.Sc. research included both theoretical and experimental development of a magneto-acoustic system for early detection of tumors labeled with magnetic nanoparticles as well as the biomedical application of hollow-core waveguides. During his Ph.D., he focused on developing an innovative multispectral photoacoustic technique for the evaluation of bone pathologies, such as osteoporosis. This new technique can simultaneously measure bone functionality and biomechanical strength.
As a recipient of a Philips Fellowship Training Award, Idan is leading the development of photoacoustic and RF-acoustic transducers for Dr. Gambhir’s Philips-supported research award of photoacoustic imaging of human prostate cancer. Those transducers are capable of performing both ultrasound imaging as well as molecular imaging of targeted agents. His work includes writing simulations for designing those devices; developing parallel reconstruction algorithms; mechanical, optical, acoustic, and electromagnetic design and manufacturing of those devices, as well as clinical trials involving those.
Idan’s long-term goals involve a research position in either academia or industry to continue his research efforts in advancing methodologies and clinical applications in biomedical optics and ultrasound.
Honors & Awards
Philips Healthcare Fellowship Training Award, Philips Healthcare (2018)
Hamalainen Pelican Postdoctoral Fellowship, Sir Peter Michael Foundation (2017 - 2020)
Contingency Student Travel Grant Award, The International Society for Optical Engineering (SPIE) (2015)
Space, Travel Scholarship for an International Conference or Workshops, Israeli Ministry of Science, Technology and Space (2015)
Research grants titled: "Optical fiber sensors for ultrasonic detection and measurement", Israeli ministry of science, technology & space \ National program for applied science (2014-2017)
Department of Physical Electronics, Best poster award - 1st place, Tel Aviv University (2014)
Travel Scholarship for an International Conference or Workshops, Israeli Ministry of Science, Technology and Space (2014)
Clore Scholars Program, Sir Charles Clore Israel Foundation (2013-2016)
Research Grant title:"Multispectral photoacoustic method for the early detection of Osteoporosis", Israeli ministry of industry, trade & labor \ Kamin program for transnational research (2013-2015)
Biophotonics graduate school - Best poster awards - 2nd Place, Biophotonics 13’, Ven Sweden (2013)
Contingency Student Travel Grant Award, The International Society for Optical Engineering (SPIE) (2013)
SPIE student chapter opening event, Best poster award - 2nd place, Tel Aviv university (2013)
Research Grant title:"A photoacoustic spectroscopy method for the early detection of Osteoporosis", The Ela Kodesz Institute for Medical engineering and physical sciences (2012)
Biophysics Fellowship, The Raymond & Beverly Sackler Biophysics Prize (2011-2014)
Outstanding teaching assistant, Tel Aviv University (2011)
Troski Scholarship, Tel Aviv University (2011)
Outstanding teaching assistant, Tel Aviv University (2010)
Faculty award for outstanding MSc students in engineering, Tel Aviv University (2009)
Faculty Scholarship, Tel Aviv University (2008)
Faculty award for outstanding undergraduate student, Tel Aviv University (2008)
Doctor of Philosophy, Tel-Aviv University (2016)
Ph.D, Tel Aviv University, faculty of engineering, Biomedical Engineering (2016)
M.Sc (Magna cum Laude), Tel Aviv University, faculty of engineering, Biomedical Engineering (with Business Administration studies) (2010)
B.Sc (Magna cum Laude), Biomedical Engineering, Tel Aviv University, faculty of engineering (2008)
Idan Steinberg, Lihi Shiloh , Haniel Gabai, Yacov Botsev, Meir Hahami and Avishay Eyal. "Israel Patent Application No. 62215052 Gated OFDR System", Sep 7, 2015
Idan Steinberg, Avishay Eyal and Israel Gannot. "Israel Patent WO 2014118781 A1 Detection, diagnosis and monitoring of osteoporosis by a photo-acoustic method", Jan 31, 2013
Current Research and Scholarly Interests
My current research is focused on developing non-ionizing and low cost medical technologies that reliably detect diagnose and monitor disease progression. I work at the interface of between Photonics, Acoustics, RF, Molecular Imaging, Medical Imaging and Biomedical Signal processing. Equal emphasis is on translating these technologies for pre-clinical and clinical applications in cancer and neurological diseases.
Low-frequency ultrasound-mediated cytokine transfection enhances T cell recruitment at local and distant tumor sites.
Proceedings of the National Academy of Sciences of the United States of America
Robust cytotoxic T cell infiltration has proven to be difficult to achieve in solid tumors. We set out to develop a flexible protocol to efficiently transfect tumor and stromal cells to produce immune-activating cytokines, and thus enhance T cell infiltration while debulking tumor mass. By combining ultrasound with tumor-targeted microbubbles, membrane pores are created and facilitate a controllable and local transfection. Here, we applied a substantially lower transmission frequency (250 kHz) than applied previously. The resulting microbubble oscillation was significantly enhanced, reaching an effective expansion ratio of 35 for a peak negative pressure of 500 kPa in vitro. Combining low-frequency ultrasound with tumor-targeted microbubbles and a DNA plasmid construct, 20% of tumor cells remained viable, and ∼20% of these remaining cells were transfected with a reporter gene both in vitro and in vivo. The majority of cells transfected in vivo were mucin 1+/CD45- tumor cells. Tumor and stromal cells were then transfected with plasmid DNA encoding IFN-β, producing 150 pg/106 cells in vitro, a 150-fold increase compared to no-ultrasound or no-plasmid controls and a 50-fold increase compared to treatment with targeted microbubbles and ultrasound (without IFN-β). This enhancement in secretion exceeds previously reported fourfold to fivefold increases with other in vitro treatments. Combined with intraperitoneal administration of checkpoint inhibition, a single application of IFN-β plasmid transfection reduced tumor growth in vivo and recruited efficacious immune cells at both the local and distant tumor sites.
View details for DOI 10.1073/pnas.1914906117
View details for PubMedID 32430322
Maltotriose-based probes for fluorescence and photoacoustic imaging of bacterial infections
View details for DOI 10.1038/s41467-020-14985-8
Simultaneous transrectal ultrasound and photoacoustic human prostate imaging.
Science translational medicine
2019; 11 (507)
Imaging technologies that simultaneously provide anatomical, functional, and molecular information are emerging as an attractive choice for disease screening and management. Since the 1980s, transrectal ultrasound (TRUS) has been routinely used to visualize prostatic anatomy and guide needle biopsy, despite limited specificity. Photoacoustic imaging (PAI) provides functional and molecular information at ultrasonic resolution based on optical absorption. Combining the strengths of TRUS and PAI approaches, we report the development and bench-to-bedside translation of an integrated TRUS and photoacoustic (TRUSPA) device. TRUSPA uses a miniaturized capacitive micromachined ultrasonic transducer array for simultaneous imaging of anatomical and molecular optical contrasts [intrinsic: hemoglobin; extrinsic: intravenous indocyanine green (ICG)] of the human prostate. Hemoglobin absorption mapped vascularity of the prostate and surroundings, whereas ICG absorption enhanced the intraprostatic photoacoustic contrast. Future work using the TRUSPA device for biomarker-specific molecular imaging may enable a fundamentally new approach to prostate cancer diagnosis, prognostication, and therapeutic monitoring.
View details for DOI 10.1126/scitranslmed.aav2169
View details for PubMedID 31462508
Photoacoustic clinical imaging.
2019; 14: 77–98
Photoacoustic is an emerging biomedical imaging modality, which allows imaging optical absorbers in the tissue by acoustic detectors (light in - sound out). Such a technique has an immense potential for clinical translation since it allows high resolution, sufficient imaging depth, with diverse endogenous and exogenous contrast, and is free from ionizing radiation. In recent years, tremendous developments in both the instrumentation and imaging agents have been achieved. These opened avenues for clinical imaging of various sites allowed applications such as brain functional imaging, breast cancer screening, diagnosis of psoriasis and skin lesions, biopsy and surgery guidance, the guidance of tumor therapies at the reproductive and urological systems, as well as imaging tumor metastases at the sentinel lymph nodes. Here we survey the various clinical and pre-clinical literature and discuss the potential applications and hurdles that still need to be overcome.
View details for DOI 10.1016/j.pacs.2019.05.001
View details for PubMedID 31293884
- First-in-Human Study of Bone Pathologies Using Low-Cost and Compact Dual-Wavelength Photoacoustic System IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS 2019; 25 (1)
An Open Source GPU-Based Beamformer for Real-Time Ultrasound Imaging and Applications
IEEE. 2019: 20–23
View details for Web of Science ID 000510220100006
Intraoperative Pancreatic Cancer Detection using Tumor-Specific Multimodality Molecular Imaging.
Annals of surgical oncology
2018; 25 (7): 1880–88
BACKGROUND: Operative management of pancreatic ductal adenocarcinoma (PDAC) is complicated by several key decisions during the procedure. Identification of metastatic disease at the outset and, when none is found, complete (R0) resection of primary tumor are key to optimizing clinical outcomes. The use of tumor-targeted molecular imaging, based on photoacoustic and fluorescence optical imaging, can provide crucial information to the surgeon. The first-in-human use of multimodality molecular imaging for intraoperative detection of pancreatic cancer is reported using cetuximab-IRDye800, a near-infrared fluorescent agent that binds to epidermal growth factor receptor.METHODS: A dose-escalation study was performed to assess safety and feasibility of targeting and identifying PDAC in a tumor-specific manner using cetuximab-IRDye800 in patients undergoing surgical resection for pancreatic cancer. Patients received a loading dose of 100mg of unlabeled cetuximab before infusion of cetuximab-IRDye800 (50mg or 100mg). Multi-instrument fluorescence imaging was performed throughout the surgery in addition to fluorescence and photoacoustic imaging ex vivo.RESULTS: Seven patients with resectable pancreatic masses suspected to be PDAC were enrolled in this study. Fluorescence imaging successfully identified tumor with a significantly higher mean fluorescence intensity in the tumor (0.09±0.06) versus surrounding normal pancreatic tissue (0.02±0.01), and pancreatitis (0.04±0.01; p<0.001), with a sensitivity of 96.1% and specificity of 67.0%. The mean photoacoustic signal in the tumor site was 3.7-fold higher than surrounding tissue.CONCLUSIONS: The safety and feasibilty of intraoperative, tumor-specific detection of PDAC using cetuximab-IRDye800 with multimodal molecular imaging of the primary tumor and metastases was demonstrated.
View details for PubMedID 29667116
Emerging Intraoperative Imaging Modalities to Improve Surgical Precision.
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging
Intraoperative imaging (IOI) is performed to guide delineation and localization of regions of surgical interest. While oncological surgical planning predominantly utilizes x-ray computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound (US), intraoperative guidance mainly remains on surgeon interpretation and pathology for confirmation. Over the past decades however, intraoperative guidance has evolved significantly with the emergence of several novel imaging technologies, including fluorescence-, Raman, photoacoustic-, and radio-guided approaches. These modalities have demonstrated the potential to further optimize precision in surgical resection and improve clinical outcomes for patients. Not only can these technologies enhance our understanding of the disease, they can also yield large imaging datasets intraoperatively that can be analyzed by deep learning approaches for more rapid and accurate pathological diagnosis. Unfortunately, many of these novel technologies are still under preclinical or early clinical evaluation. Organizations like the Intra-Operative Imaging Study Group of the European Society for Molecular Imaging (ESMI) support interdisciplinary interactions with the aim to improve technical capabilities in the field, an approach that can succeed only if scientists, engineers, and physicians work closely together with industry and regulatory bodies to resolve roadblocks to clinical translation. In this review, we provide an overview of a variety of novel IOI technologies, discuss their challenges, and present future perspectives on the enormous potential of IOI for oncological surgical navigation.
View details for PubMedID 29916118
A Reconstruction Method for the Estimation of Temperatures of Multiple Sources Applied for Nanoparticle-Mediated Hyperthermia
2018; 23 (3)
Solid malignant tumors are one of the leading causes of death worldwide. Many times complete removal is not possible and alternative methods such as focused hyperthermia are used. Precise control of the hyperthermia process is imperative for the successful application of such treatment. To that end, this research presents a fast method that enables the estimation of deep tissue heat distribution by capturing and processing the transient temperature at the boundary based on a bio-heat transfer model. The theoretical model is rigorously developed and thoroughly validated by a series of experiments. A 10-fold improvement is demonstrated in resolution and visibility on tissue mimicking phantoms. The inverse problem is demonstrated as well with a successful application of the model for imaging deep-tissue embedded heat sources. Thereby, allowing the physician then ability to dynamically evaluate the hyperthermia treatment efficiency in real time.
View details for PubMedID 29547502
- Deep Tissue Imaging: Acoustic and Thermal Wave Propagation and Light Interactions in Tissues Deep Imaging in Tissue and Tissue-Like Media with Linear and Nonlinear Optics CRC Press. 2017
Quantitative study of optical and mechanical bone status using multispectral photoacoustics.
Journal of biophotonics
2016; 9 (9): 924-933
Osteoporosis is a major public health problem worldwide. Here, we present a quantitative multispectral photoacoustic method for the evaluation of bone pathologies which has significant advantages over pure ultrasonic or pure optical methods as it provides both molecular information and bone mechanical status. This is enabled via a simultaneous measurement of the bone's optical properties as well as the speed of sound and ultrasonic attenuation in the bone. To test the method's quantitative predictions, a combined ultrasonic and photoacoustic system was developed. Excitation was performed optically via a portable triple laser-diode system and acoustically via a single element transducer. Additional dual transducers were used for detecting the acoustic waves that were generated by the two modalities. Both temporal and spectral parameters were compared between different excitation wavelengths and measurement modalities. Short photoacoustic excitation wavelengths allowed sensing of the cortical layer while longer wavelengths produced results which were compatible with the quantitative ultrasound measurements.
View details for DOI 10.1002/jbio.201500206
View details for PubMedID 26487250
- A Route to Laser Angioplasty in the Presence of Fluoroscopy Contrast Media, Using a Nanosecond-Pulsed 355-nm Laser IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS 2016; 22 (3)
- All fiber sensor array for ultrasound sensing PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2016 2016; 9708
Shaping photomechanical effects in tissue ablation using 355 nm laser pulses
Journal of Biophotonics
View details for DOI 10.1002/jbio.201600094
Multiplexing of fiber-optic ultrasound sensors via swept frequency interferometry
2015; 23 (15): 18915-18924
The use of fiber-optic sensors for ultrasound (US) detection has many advantages over conventional piezoelectric detectors. However, the issue of multiplexing remains a major challenge. Here, a novel approach for multiplexing fiber-optic based US sensors using swept frequency interferometry is introduced. Light from a coherent swept source propagates in an all-fiber interferometric network made of a reference arm and a parallel connection of N sensing arms. Each sensing arm comprises a short polyimide coated sensing section (~4cm), which is exposed to the US excitation, preceded by a delay of different length. When the instantaneous frequency of the laser is linearly swept, the receiver output contains N harmonic beat components which correspond to the various optical paths. Exposing the sensing sections to US excitation introduces phase modulation of the harmonic components. The US-induced signals can be separated in the frequency domain and be extracted from their carriers by common demodulation techniques. The method was demonstrated by multiplexing 4 sensing fibers and detecting microsecond US pulses which were generated by a 2.25MHz ultrasound transducer. The pulses were successfully measured by all sensing fibers without noticeable cross-talk.
View details for DOI 10.1364/OE.23.018915
View details for Web of Science ID 000361035300034
View details for PubMedID 26367554
- Over 100km long ultra-sensitive dynamic sensing via Gated-OFDR 24TH INTERNATIONAL CONFERENCE ON OPTICAL FIBRE SENSORS 2015; 9634
- All-fiber ultrasound sensor array implemented by swept frequency interferometry 24TH INTERNATIONAL CONFERENCE ON OPTICAL FIBRE SENSORS 2015; 9634
- Investigation of a Dual modal method for bone pathologies using quantitative ultrasound and Photoacoustics PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2015 2015; 9323
- Broadband ultrasonic sensor array via optical frequency domain reflectometry PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2015 2015; 9323
Tumor Localization Using Magnetic Nanoparticle-Induced Acoustic Signals
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
2014; 61 (8): 2313-2323
Cancer is a major public health problem worldwide, especially in developed countries. Early detection of cancer can greatly increase both survival rates and quality of life for patients. A magnetoacoustic-based method had been previously proposed for early tumor detection, in a minimal invasive procedure, using magnetic nanoparticles (MNPs). This paper presents a supporting localization algorithm that can provide the clinician with essential tumor location data and could enable a sequential biopsy. It provides localization algorithm development, as well as its validation in both computerized simulations and in vitro experiments. Three-dimensional (3-D) tumor localization is demonstrated with an error of 2.14 mm and an overlapping volume of 84% of the actual tumor. The obtained results are promising and prove the feasibility of tumor localization using a time difference of arrival algorithm along with a magnetoacoustic detection scheme.
View details for DOI 10.1109/TBME.2013.2286638
View details for Web of Science ID 000340259900009
View details for PubMedID 24158469
Robust estimation of cerebral hemodynamics in neonates using multilayered diffusion model for normal and oblique incidences
JOURNAL OF BIOMEDICAL OPTICS
2014; 19 (7)
The diffusion approximation is useful for many optical diagnostics modalities, such as near-infrared spectroscopy. However, the simple normal incidence, semi-infinite layer model may prove lacking in estimation of deep-tissue optical properties such as required for monitoring cerebral hemodynamics, especially in neonates. To answer this need, we present an analytical multilayered, oblique incidence diffusion model. Initially, the model equations are derived in vector-matrix form to facilitate fast and simple computation. Then, the spatiotemporal reflectance predicted by the model for a complex neonate head is compared with time-resolved Monte Carlo (TRMC) simulations under a wide range of physiologically feasible parameters. The high accuracy of the multilayer model is demonstrated in that the deviation from TRMC simulations is only a few percent even under the toughest conditions. We then turn to solve the inverse problem and estimate the oxygen saturation of deep brain tissues based on the temporal and spatial behaviors of the reflectance. Results indicate that temporal features of the reflectance are more sensitive to deep-layer optical parameters. The accuracy of estimation is shown to be more accurate and robust than the commonly used single-layer diffusion model. Finally, the limitations of such approaches are discussed thoroughly.
View details for DOI 10.1117/1.JBO.19.7.071406
View details for Web of Science ID 000340490400011
View details for PubMedID 24604607
Monitoring LITT thermal penetration depth using real-time analysis of backscattered light
JOURNAL OF BIOPHOTONICS
2014; 7 (6): 381-391
Real-time monitoring of the thermal penetration depth (TPD) is essential in various clinical procedures, such as Laser Interstitial Thermal Therapy (LITT). MRI is commonly used to this end, though bulky and expensive. In this paper, we present an alternative novel method for an optical feedback system based on changes in the diffused reflection from the tissue during treatment. Monte-Carlo simulation was used to deduce the relations between the backscattered pattern and the TPD. Several methods of image analysis are developed for TPD estimation. Each yields a set of parameters which are linearly dependent on the TPD. In order to test these experimentally, tissue samples were monitored in-vitro during treatment at multiple wavelengths. The SNR and coefficient of determination were used to compare the various methods and wavelengths and to determine the preferred method. Such system and algorithms may be used for real-time in-vivo control during laser thermotherapy and other clinical procedures.
View details for DOI 10.1002/jbio.201200082
View details for Web of Science ID 000337699300003
View details for PubMedID 23192946
- Theoretical and experimental investigation of multispectral photoacoustic Osteoporosis detection method PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2014 2014; 8943
- Multispectral photoacoustic method for the early detection and diagnosis of osteoporosis PHOTONIC THERAPEUTICS AND DIAGNOSTICS IX 2013; 8565
A new method for tumor detection using induced acoustic waves from tagged magnetic nanoparticles
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
2012; 8 (5): 569-579
Magnetoacoustic detection is a new method for the noninvasive, early detection of cancer. It uses specific superparamagnetic nanoparticles (NPs) that bind to tumor sites together with magnetic excitation and acoustic detection of the tumor-NPs complex. This work tests the feasibility of such method theoretically and experimentally. An extensive analytic model has been developed that shows an ability to detect small tumors, a few centimeters deep inside the tissue. A series of experiments were conducted to validate the theoretical model. The performance of specially designed solenoids was measured, and the detection of the tumor presence in phantom was demonstrated. Experimental results agree well with the theoretical calculations, providing preliminary proof of concept. We demonstrate the ability to detect a 5-mm diameter spherical tumor located 3 cm deep. Instrumentation and measurements are inexpensive and accurate. The accuracy, speed, and costs of this method show the potential for early detection of cancer.A sensitive and cost effective magentoacoustic tumor detection method is presented in this paper using superparamagnetic nanoparticles. The method is demonstrated in a phantom by detecting a 5-mm diameter spherical tumor located 3 cm deep.
View details for DOI 10.1016/j.nano.2011.09.011
View details for Web of Science ID 000305704800004
View details for PubMedID 22024194
Multilayer Mie scattering model for investigation of intracellular structural changes in the nucleolus and cytoplasm
International Journal of Optics
View details for DOI 10.1155/2012/947607
Time difference of arrival based cancer tumor localization using magnetic nanoparticles induced acoustic signals
View details for DOI 10.1557/opl.2012.1533
- The Role of Skew Rays in Biomedical Sensing IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS 2010; 16 (4): 961-966
Ultrashort Laser-Pulse Medical Imaging
Encyclopedia of Analytical Chemistry,
Wiley Online Library. 2010
View details for DOI 10.1002/9780470027318
- HOLLOW CORE WAVEGUIDES FOR RADIATION DELIVERY AND SENSING: MONTE CARLO, RAY TRACING COMPUTER SIMULATION OPTICAL FIBERS AND SENSORS FOR MEDICAL DIAGNOSTICS AND TREATMENT APPLICATIONS IX 2009; 7173