Bio


Ivana Martinić is currently a postdoctoral research fellow in the department of radiology at Stanford University. She received her B.Sc. and M.Sc. degrees in Molecular Biotechnology from University of Zagreb (Croatia), in 2011 and 2013, and M.Sc. degree in Bioindustrial Techniques from University of Orléans (France), in 2013. Her research during M.Sc. was focused on: `Exploring of a New Nuclear mRNA Surveillance Pathway in Saccharomyces Cerevisiae`. She received her PhD degree in biology/biochemistry in 2016 at the University of Orléans (France) under supervision of Prof. Stéphane Petoud and Dr. Svetlana V. Eliseeva and support of a Marie Curie fellowship. Her research interests were focused on development of near-infrared (NIR) optical imaging agents based on lanthanide ions (e.g. small molecules, polymetallic complexes, Metal Organic Frameworks (MOFs), polyamidoamine dendrimers and nanoparticles) for applications in cancer research and diagnostics. As a complement and continuation of her PhD work she has worked on a maturation project financially supported by the Technology Transfer Company (SATT Grand Centre, France) that has the main goal to create and characterize imaging agents as commercially valuable products. She is co-inventor of five patents and has published a few peer-reviewed papers in high ranking journals and gave several talks at international conferences.

Honors & Awards


  • Poster award at European Molecular Imaging Meeting for `Optical, Optoacoustic and Ultrasound Probes`, European Society for Molecular Imaging (ESMI) (07/04/2017)
  • Fellowship award for a Ph.D. research, Marie Curie Innovative Training Network (09/01/2013 - 08/31/2016)

Professional Education


  • Master of Engineering, Universite D'Orleans (2013)
  • Master of Science, Universite D'Orleans (2013)
  • Doctor of Philosophy, Universite D'Orleans (2016)

Patents


  • S. Petoud, F. Suzenet, R. Delatouche, S.V. Eliseeva, V. Placide, J. Leclerc, I. Martinić, G. Collet. "France Patent Invention Disclosure DI 7323-01 NIR Sensibilisation of NIR Lanthanides on a Dendrimeric Plateform and their Vectorization", National Center for Scientific Research in France
  • S. Petoud, S.V. Eliseeva, F. Suzenet, S. Routier, I. Martinić, R. Ben Othman, R. Delatouche, A. Foucault-Collet. "France Patent Invention Disclosure DI 7323-02 Chromophoric Groups Based on Anthraquinones Moieties Attached to Derivatives of Triethylenetetramine-N,N,N',N", National Center for Scientific Research in France
  • V.L. Pecoraro, S.V. Eliseeva, S. Petoud, I. Martinić, T. Nguyen. "United States Patent 2017029242 Simultaneous Cell Fixing and Staining with Metallacrown Complexes", University of Michigan and National Center for Scientific Research in France, Feb 23, 2017
  • E.R. Trivedi, V.L. Pecoraro, S.V. Eliseeva, S. Petoud, J. Jankolovits, I. Martinić, A. Foucault Collet. "United States Patent 20160215001 Metallacrown Complexes and Methods of Making the Same", University of Michigan and National Center for Scientific Research in France, Jul 28, 2016
  • E.R. Trivedi, V.L. Pecoraro, S.V. Eliseeva, S. Petoud, C.Y. Chow, J.C. Lutter, T. Nguyen, I. Martinić. "United States Patent 2015035196 Ln(III) and Ga(III) Metallacrown Complexes", University of Michigan and National Center for Scientific Research in France, Mar 12, 2015

All Publications


  • One-Step Assembly of Visible and Near-Infrared Emitting Metallacrown Dimers Using a Bifunctional Linker CHEMISTRY-A EUROPEAN JOURNAL Nguyen, T. N., Chow, C. Y., Eliseeva, S. V., Trivedi, E. R., Kampf, J. W., Martinic, I., Petoud, S., Pecoraro, V. L. 2018; 24 (5): 1031–35

    Abstract

    A family of dimeric LnIII [12-MCGa(III)N(shi) -4] metallacrowns (MCs) (LnIII =Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, and Yb) was synthesized using the isophthalate group (ip2- ) as a linker. The [LnGa4 ]2 complexes exhibit remarkable photophysical properties, with large molar absorptivities of ≈4×104  m-1  cm-1 , high quantum yields and long luminescence lifetimes with values of (i) 31.2(2)% and 1.410(1) ms, respectively for the visible-emitting [TbGa4 ]2 complex and (ii) 2.43(6)% and 30.5(1) μs for the near-infrared (NIR) emitting [YbGa4 ]2 in the solid state. The NIR emission was obtained not only from Yb, Nd, and Er complexes but also from the less frequently observed emitters such as Pr and Ho. In addition, emission in both visible and NIR domains could be detected for Dy and Sm MCs. ESI-MS and UV/Vis data revealed that the complexes are highly stable in dimethylsulfoxide (DMSO) solution with the 1 H- and COSY-NMR spectra of the diamagnetic [YGa4 ]2 analogue providing evidence for long-term solution stability. This new approach allows one to construct a basis for highly luminescent MCs that may be further modified to be adapted for applications such as optical imaging.

    View details for DOI 10.1002/chem.201703911

    View details for Web of Science ID 000427316700005

    View details for PubMedID 28960536

  • Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining CHEMICAL SCIENCE Martinic, I., Eliseeva, S. V., Nguyen, T. N., Foucher, F., Gosset, D., Westall, F., Pecoraro, V. L., Petoud, S. 2017; 8 (9): 6042–50

    Abstract

    Cell fixation is an essential approach for preserving cell morphology, allowing the targeting and labelling of biomolecules with fluorescent probes. One of the key requirements for more efficient fluorescent labelling is the preservation of cell morphology, which usually requires a combination of several fixation techniques. In addition, the use of a counter stain is often essential to improve the contrast of the fluorescent probes. Current agents possess significant limitations, such as low resistance toward photobleaching and sensitivity to changes in the microenvironment. Luminescent Ln3+ 'encapsulated sandwich' metallacrowns (MCs) overcome these drawbacks and offer complementary advantages. In particular, they emit sharp emission bands, possess a large difference between excitation and emission wavelengths and do not photobleach. Herein, MCs formed with pyrazinehydroxamic acid (Ln3+[Zn(ii)MCpyzHA], Ln3+ = Yb, Nd) were used, combined with near-infrared (NIR) counter staining and fixation agents for HeLa cells upon an initial five minute exposure to UV-A light. The validity and quality of the cell fixation were assessed with Raman spectroscopy. Analysis of the NIR luminescence properties of these MCs was performed under different experimental conditions, including in a suspension of stained cells. Moreover, the high emission intensity of Ln3+[Zn(ii)MCpyzHA] in the NIR region allows these MCs to be used for imaging with standard CCD cameras installed on routine fluorescence microscopes. Finally, the NIR-emitting Ln3+[Zn(ii)MCpyzHA] compounds combine, within a single molecule, features such as cell fixation and staining abilities, good photostability and minimal sensitivity of the emission bands to the local microenvironment, and they are highly promising for establishing the next generation of imaging agents with a single biodistribution.

    View details for DOI 10.1039/c7sc01872j

    View details for Web of Science ID 000408168600019

    View details for PubMedID 28989634

    View details for PubMedCentralID PMC5625569

  • Near-infrared emitting probes for biological imaging: Organic fluorophores, quantum dots, fluorescent proteins, lanthanide(III) complexes and nanomaterials JOURNAL OF LUMINESCENCE Martinic, I., Eliseeva, S. V., Petoud, S. 2017; 189: 19–43
  • Near-Infrared Optical Imaging of Necrotic Cells by Photostable Lanthanide-Based Metallacrowns JOURNAL OF THE AMERICAN CHEMICAL SOCIETY Martinic, I., Eliseeva, S. V., Nguyen, T. N., Pecoraro, V. L., Petoud, S. 2017; 139 (25): 8388–91

    Abstract

    Sensitive detection of cell necrosis is crucial for the determination of cell viability. Because of its high resolution at the cellular level and sensitivity, optical imaging is highly attractive for identifying cell necrosis. However, challenges associated with this technique remain present such as the rapid photobleaching of several types of organic fluorophores and/or the interference generated by biological autofluorescence. Herein, we synthesized novel biologically compatible Zn2+/Ln3+ metallacrowns (MCs) that possess attractive near-infrared (NIR) emission and are highly photostable. In addition, these MCs have the ability to label differentially necrotic HeLa cells from living cells. This work is also the first demonstration of (i) the use of the NIR emission arising from a single lanthanide(III) cation for optical biological imaging of cells under single photon excitation, (ii) the first example of a lanthanide(III)-based NIR-emitting probe that can be targeted to a specific type of cell.

    View details for DOI 10.1021/jacs.7b01587

    View details for Web of Science ID 000404809600002

    View details for PubMedID 28613848

  • Growth Inhibition Of Mesenchymal Stem Cells By Laminarin Definitions Larguech, G., Cesaro, A., Toumi, H., Martinic, I., Petout, S., Daniellou, R., Lespessailles, E. LIPPINCOTT WILLIAMS & WILKINS. 2017: 803