Professional Education


  • BS, Stanford University, Biological Sciences (2008)
  • MPhil, University of Cambridge, Computational Biology (2010)
  • PhD, University of Washington, Molecular and Cellular Biology (2017)
  • MD, University of Washington (2019)

Stanford Advisors


All Publications


  • Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics NATURE METHODS Rose, J. C., Stephany, J. J., Valente, W. J., Trevillian, B. M., Dang, H. V., Bielas, J. H., Maly, D. J., Fowler, D. M. 2017; 14 (9): 891-+

    Abstract

    We developed a chemically inducible Cas9 (ciCas9) and a droplet digital PCR assay for double-strand breaks (DSB-ddPCR) to investigate the kinetics of Cas9-mediated generation and repair of DSBs in cells. ciCas9 is a rapidly activated, single-component Cas9 variant engineered by replacing the protein's REC2 domain with the BCL-xL protein and fusing an interacting BH3 peptide to the C terminus. ciCas9 can be tunably activated by a compound that disrupts the BCL-xL-BH3 interaction within minutes. DSB-ddPCR demonstrates time-resolved, highly quantitative, and targeted measurement of DSBs. Combining these tools facilitated an unprecedented exploration of the kinetics of Cas9-mediated DNA cleavage and repair. We find that sgRNAs targeting different sites generally induce cleavage within minutes and repair within 1 or 2 h. However, we observe distinct kinetic profiles, even for proximal sites, and this suggests that target sequence and chromatin state modulate cleavage and repair kinetics.

    View details for DOI 10.1038/NMETH.4368

    View details for Web of Science ID 000408776400018

    View details for PubMedID 28737741

    View details for PubMedCentralID PMC5730411

  • A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics NATURE CHEMICAL BIOLOGY Rose, J. C., Huang, P., Camp, N. D., Ye, J., Leidal, A. M., Goreshnik, I., Trevillian, B. M., Dickinson, M. S., Cunningham-Bryant, D., Debnath, J., Baker, D., Wolf-Yadlin, A., Maly, D. J. 2017; 13 (1): 119-126

    Abstract

    Synthetic protein switches controlled with user-defined inputs are powerful tools for studying and controlling dynamic cellular processes. To date, these approaches have relied primarily on intermolecular regulation. Here we report a computationally guided framework for engineering intramolecular regulation of protein function. We utilize this framework to develop chemically inducible activator of RAS (CIAR), a single-component RAS rheostat that directly activates endogenous RAS in response to a small molecule. Using CIAR, we show that direct RAS activation elicits markedly different RAS-ERK signaling dynamics from growth factor stimulation, and that these dynamics differ among cell types. We also found that the clinically approved RAF inhibitor vemurafenib potently primes cells to respond to direct wild-type RAS activation. These results demonstrate the utility of CIAR for quantitatively interrogating RAS signaling. Finally, we demonstrate the general utility of our approach in design of intramolecularly regulated protein tools by applying it to the Rho family of guanine nucleotide exchange factors.

    View details for DOI 10.1038/NGHEMBIO.2244

    View details for Web of Science ID 000393267200022

    View details for PubMedID 27870838

    View details for PubMedCentralID PMC5161653

  • DIFFERENTIAL LOCALIZATION OF AN ENGINEERED RAS RHEOSTAT REVEALS UNIQUE RAS-ERK SIGNALING DYNAMICS Dieter, E. M., Rose, J., Maly, D. WILEY. 2019: 158–59
  • A Chemically Disrupted Proximity System for Controlling Dynamic Cellular Processes JOURNAL OF THE AMERICAN CHEMICAL SOCIETY Cunningham-Bryant, D., Dieter, E. M., Foight, G. W., Rose, J. C., Loutey, D. E., Maly, D. J. 2019; 141 (8): 3352–55

    Abstract

    Chemical methods that allow the spatial proximity of proteins to be temporally modulated are powerful tools for studying biology and engineering synthetic cellular behaviors. Here, we describe a new chemically controlled method for rapidly disrupting the interaction between two basally colocalized protein binding partners. Our chemically disrupted proximity (CDP) system is based on the interaction between the hepatitis C virus protease (HCVp) NS3a and a genetically encoded peptide inhibitor. Using clinically approved antiviral inhibitors as chemical disrupters of the NS3a/peptide interaction, we demonstrate that our CDP system can be used to confer temporal control over diverse intracellular processes. This NS3a-based CDP system represents a new modality for engineering chemical control over intracellular protein function that is complementary to currently available techniques.

    View details for DOI 10.1021/jacs.8b12382

    View details for Web of Science ID 000460200100004

    View details for PubMedID 30735038

    View details for PubMedCentralID PMC6492022

  • "Examining RAS pathway rewiring with a chemically inducible activator of RAS". Small GTPases Rose, J. C., Dieter, E. M., Cunningham-Bryant, D., Maly, D. J. 2018: 1–8

    Abstract

    RAS signaling pathways govern diverse cellular processes, are dynamic, and exhibit marked plasticity. Yet, these features also present a considerable obstacle to their study. Here, we report the use of a recently described RAS rheostat, Chemically Inducible Activator of RAS (CIAR), to study two poorly understood phenomena in RAS biology. First, we show that short-term activation of wild type endogenous RAS can desensitize cells to EGF stimulation. Second, we examine the phenomena of paradoxical activation of RAS/ERK signaling by RAF inhibitors. Specifically, we characterize the effects on RAS/ERK signaling kinetics of four RAF inhibitors, which stabilize distinct ATP-binding site conformations. These results demonstrate the utility of CIAR in conducting quantitative studies of complex features of RAS biology.

    View details for DOI 10.1080/21541248.2018.1446697

    View details for PubMedID 29634387

  • PAZOPANIB-INDUCED RHABDOMYOLYSIS IN THE SETTING OF HYPOTHYROIDISM Crooks, C. P., Ghiathi, C., Rose, J. C., Redinger, J. SPRINGER. 2018: S585
  • Rheostatic Control of Cas9-Mediated DNA Double Strand Break (DSB) Generation and Genome Editing ACS CHEMICAL BIOLOGY Rose, J. C., Stephany, J. J., Wei, C. T., Fowler, D. M., Maly, D. J. 2018; 13 (2): 438–42

    Abstract

    We recently reported two novel tools for precisely controlling and quantifying Cas9 activity: a chemically inducible Cas9 variant (ciCas9) that can be rapidly activated by small molecules and a ddPCR assay for time-resolved measurement of DNA double strand breaks (DSB-ddPCR). Here, we further demonstrate the potential of ciCas9 to function as a tunable rheostat for Cas9 function. We show that a new highly potent and selective small molecule activator paired with a more tightly regulated ciCas9 variant expands the range of accessible Cas9 activity levels. We subsequently demonstrate that ciCas9 activity levels can be dose-dependently tuned with a small molecule activator, facilitating rheostatic time-course experiments. These studies provide the first insight into how Cas9-mediated DSB levels correlate with overall editing efficiency. Thus, we demonstrate that ciCas9 and our DSB-ddPCR assay permit the time-resolved study of Cas9 DSB generation and genome editing kinetics at a wide range of Cas9 activity levels.

    View details for DOI 10.1021/acschembio.7b00652

    View details for Web of Science ID 000426012800020

    View details for PubMedID 28895730

    View details for PubMedCentralID PMC5821106

  • Principles of Systems Biology, No. 14 CELL SYSTEMS Erez, Z., Sorek, R., Shao, S., Hegde, R. S., Schweppe, D. K., Chavez, J. D., Bruce, J. E., Mizrachi, E., Verbeke, L., de Peer, Y., Marchal, K., Myburg, A. A., Bennett, J. A., Klironomos, J., Payne, G. F., Bentley, W. E., Fleming, R., Thiele, I., Cheng, J. K., Alper, H. S., Rose, J., Maly, D., Piraner, D., Abedi, M., Shapiro, M., Cui, Z., Mureev, S., Alexandrov, K. 2017; 4 (2): 140–43

    Abstract

    This month: sage advice from phage to their offspring; systematic analyses of protein quality control, mitochondrial respiration, and woody biomass; a continental-scale experiment; and engineered protein tools galore.

    View details for Web of Science ID 000395786100002

    View details for PubMedID 28231445

  • Mechanisms of resistance to cabazitaxel. Molecular cancer therapeutics Duran, G. E., Wang, Y. C., Francisco, E. B., Rose, J. C., Martinez, F. J., Coller, J., Brassard, D., Vrignaud, P., Sikic, B. I. 2015; 14 (1): 193-201

    Abstract

    We studied mechanisms of resistance to the novel taxane cabazitaxel in established cellular models of taxane resistance. We also developed cabazitaxel-resistant variants from MCF-7 breast cancer cells by stepwise selection in drug alone (MCF-7/CTAX) or drug plus the transport inhibitor PSC-833 (MCF-7/CTAX-P). Among multidrug-resistant (MDR) variants, cabazitaxel was relatively less cross-resistant than paclitaxel and docetaxel (15- vs. 200-fold in MES-SA/Dx5 and 9- vs. 60-fold in MCF-7/TxT50, respectively). MCF-7/TxTP50 cells that were negative for MDR but had 9-fold resistance to paclitaxel were also 9-fold resistant to cabazitaxel. Selection with cabazitaxel alone (MCF-7/CTAX) yielded 33-fold resistance to cabazitaxel, 52-fold resistance to paclitaxel, activation of ABCB1, and 3-fold residual resistance to cabazitaxel with MDR inhibition. The MCF-7/CTAX-P variant did not express ABCB1, nor did it efflux rhodamine-123, BODIPY-labeled paclitaxel, and [(3)H]-docetaxel. These cells are hypersensitive to depolymerizing agents (vinca alkaloids and colchicine), have reduced baseline levels of stabilized microtubules, and impaired tubulin polymerization in response to taxanes (cabazitaxel or docetaxel) relative to MCF-7 parental cells. Class III β-tubulin (TUBB3) RNA and protein were elevated in both MCF-7/CTAX and MCF-7/CTAX-P. Decreased BRCA1 and altered epithelial-mesenchymal transition (EMT) markers are also associated with cabazitaxel resistance in these MCF-7 variants, and may serve as predictive biomarkers for its activity in the clinical setting. In summary, cabazitaxel resistance mechanisms include MDR (although at a lower level than paclitaxel and docetaxel), and alterations in microtubule dynamicity, as manifested by higher expression of TUBB3, decreased BRCA1, and by the induction of EMT. Mol Cancer Ther; 14(1); 193-201. ©2014 AACR.

    View details for DOI 10.1158/1535-7163.MCT-14-0155

    View details for PubMedID 25416788

  • HTSanalyzeR: an R/Bioconductor package for integrated network analysis of high-throughput screens BIOINFORMATICS Wang, X., Terfve, C., Rose, J. C., Markowetz, F. 2011; 27 (6): 879–80

    Abstract

    High-throughput screens (HTS) by RNAi or small molecules are among the most promising tools in functional genomics. They enable researchers to observe detailed reactions to experimental perturbations on a genome-wide scale. While there is a core set of computational approaches used in many publications to analyze these data, a specialized software combining them and making them easily accessible has so far been missing.Here we describe HTSanalyzeR, a flexible software to build integrated analysis pipelines for HTS data that contains over-representation analysis, gene set enrichment analysis, comparative gene set analysis and rich sub-network identification. HTSanalyzeR interfaces with commonly used pre-processing packages for HTS data and presents its results as HTML pages and network plots.Our software is written in the R language and freely available via the Bioconductor project at http://www.bioconductor.org.

    View details for DOI 10.1093/bioinformatics/btr028

    View details for Web of Science ID 000288277300025

    View details for PubMedID 21258062

    View details for PubMedCentralID PMC3051329

  • The expression of activator protein 2 (AP-2) transcription factors in MDR1(+) and MDR1(-) taxane resistant human breast carcinoma variants. Rose, J., Duran, G., Sikic, B. AMER ASSOC CANCER RESEARCH. 2009