Stanford Advisors


All Publications


  • Highly sensitive detection of DNA hypermethylation in melanoma cancer cells. Biosensors & bioelectronics Nesvet, J., Rizzi, G., Wang, S. X. 2018; 124-125: 136–42

    Abstract

    Aberrant hypermethylation of CpG islands in the promoter region of tumor suppressor genes is a promising biomarker for early cancer detection. This methylation status is reflected in the methylation pattern of ctDNA shed from the primary tumor; however, to realize the full clinical utility of ctDNA methylation detection via liquid biopsy for early cancer diagnosis, improvements in the sensitivity and multiplexability of existing technologies must be improved. Additionally, the assay must be cheap and easy to perform in a clinical setting. We report the integration of methylation specific PCR (MSP) to melt curve analysis on giant magnetoresistive (GMR) biosensors to greatly enhance the sensitivity of our DNA hybridization assay for methylation detection. Our GMR sensor is functionalized with synthetic DNA probes that target methylated or unmethylated CpG sites in the MSP amplicon, and measures the difference in melting temperature (Tm) between the two probes (DeltaTm), giving an analytical limit of detection down to 0.1% methylated DNA in solution. Additionally, linear regression of DeltaTm's for serial dilutions of methylated:unmethylated mixtures allows for quantification of methylation percentage, which could have diagnostic and prognostic utility. Lastly, we performed multiplexed MSP on two different genes, and show the ability of our GMR assay to resolve this mixture, despite their amplicons' overlapping Tm's in standard EvaGreen melt analysis. The multiplexing ability of our assay and its enhanced sensitivity, without necessitating deep sequencing, represent important steps toward realizing an assay for the detection of methylated ctDNA in plasma for early cancer detection in a clinical setting.

    View details for PubMedID 30366258

  • Multigene profiling of single circulating tumor cells. Molecular & cellular oncology Park, S., Wong, D. J., Ooi, C. C., Nesvet, J. C., Nair, V. S., Wang, S. X., Gambhir, S. S. 2017; 4 (2)

    Abstract

    Numerous techniques for isolating circulating tumor cells (CTCs) have been developed. Concurrently, single-cell techniques that can reveal molecular components of CTCs have become widely available. We discuss how the combination of isolation and multigene profiling of single CTCs in our platform can facilitate eventual translation to the clinic.

    View details for DOI 10.1080/23723556.2017.1289295

    View details for PubMedID 28401190

    View details for PubMedCentralID PMC5383366

  • Multigene Profiling of Single Circulating Tumor Cells Molecular & Cellular Oncology Park, S., Wong, D., Ooi, C., Nesvet, J., Nair, V. S., Wang, S. X., Gambhir, S. S. 2017; 4 (2): e1289295

    Abstract

    Numerous techniques for isolating circulating tumor cells (CTCs) have been developed. Concurrently, single-cell techniques that can reveal molecular components of CTCs have become widely available. We discuss how the combination of isolation and multigene profiling of single CTCs in our platform can facilitate eventual translation to the clinic.

    View details for DOI 10.1080/23723556.2017.1289295

    View details for PubMedCentralID PMC5383366