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  • Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins STRUCTURAL PROTEOMICS, 3 EDITION Luo, Y., Xiang, S., Paioni, A., Adler, A., Hooikaas, P., Jijumon, A. S., Janke, C., Akhmanova, A., Baldus, M., Owens, R. J. 2021; 2305: 193-201


    In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and functional assembly of MTs and MT-MAP complexes, we discuss solid-state NMR approaches, including fast MAS and hyperpolarization methods that can be used to examine these systems. Such studies can provide novel insight into the dynamic properties of MTs and MT-MAP complexes.

    View details for DOI 10.1007/978-1-0716-1406-8_10

    View details for Web of Science ID 000691017300011

    View details for PubMedID 33950391