Honors & Awards
Postdoctoral Young Investigator Award, Stanford Institute for Stem Cell Biology and Regenerative Medicine (11/11/2018)
School of Medicine Dean's Postdoctoral Fellowship, School of Medicine, Stanford University (1/1/2018)
Doctor of Philosophy, University of Notre Dame (2017)
Bachelor of Applied Science, Unlisted School (2009)
Cell cycle dynamics of human pluripotent stem cells primed for differentiation.
Stem cells (Dayton, Ohio)
Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator (FUCCI) system adapted into hPSCs and perform RNA-sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs toward differentiation. SIGNIFICANCE STATEMENT: Generating differentiated cell types from human pluripotent stem cells (hPSCs) holds great therapeutic promise, but has proven to be challenging in practice. The cell cycle may play an important role in enhancing the differentiation potential of hPSCs. Here, the authors track and isolate hPSCs from different phases of the cell cycle and perform RNA-sequencing. The data show that gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner as hPSCs transition toward differentiation and highlight an important role for PI3K signaling in regulating these early transitory states. © AlphaMed Press 2019.
View details for DOI 10.1002/stem.3041
View details for PubMedID 31135093
Transient treatment of human pluripotent stem cells with DMSO to promote differentiation
View details for DOI 10.3791/59833
A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family
View details for DOI 10.1371/journal.pone.0208110