Honors & Awards


  • The Walter V. and Idun Berry Postdoctoral Fellowship, Lucile Packard Foundation for Children's Health, Stanford University (2017/09 - present)
  • Dean's Postdoctoral Fellowship, Stanford School of Medicine (2016/07-2017/06)
  • Pelotonia Graduate Student Fellowship, The James Cancer Hospital at The Ohio State University (2013 - 2015)

Professional Education


  • Doctor of Philosophy, Ohio State University (2015)
  • Bachelor of Science, Wuhan University (2008)

Stanford Advisors


All Publications


  • Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions RNA Foretek, D., Wu, J., Hopper, A. K., Bogota, M. 2016; 22 (3): 339-349
  • Genome-wide screen uncovers novel pathways for tRNA processing and nuclear-cytoplasmic dynamics GENES & DEVELOPMENT Wu, J., Bao, A., Chatterjee, K., Wan, Y., Hopper, A. K. 2015; 29 (24): 2633-2644

    Abstract

    Transfer ribonucleic acids (tRNAs) are essential for protein synthesis. However, key gene products involved in tRNA biogenesis and subcellular movement remain to be discovered. We conducted the first comprehensive unbiased analysis of the role of nearly an entire proteome in tRNA biology and describe 162 novel and 12 previously known Saccharomyces cerevisiae gene products that function in tRNA processing, turnover, and subcellular movement. tRNA nuclear export is of particular interest because it is essential, but the known tRNA exporters (Los1 [exportin-t] and Msn5 [exportin-5]) are unessential. We report that mutations of CRM1 (Exportin-1), MEX67/MTR2 (TAP/p15), and five nucleoporins cause accumulation of unspliced tRNA, a hallmark of defective tRNA nuclear export. CRM1 mutation genetically interacts with los1Δ and causes altered tRNA nuclear-cytoplasmic distribution. The data implicate roles for the protein and mRNA nuclear export machineries in tRNA nuclear export. Mutations of genes encoding actin cytoskeleton components and mitochondrial outer membrane proteins also cause accumulation of unspliced tRNA, likely due to defective splicing on mitochondria. Additional gene products, such as chromatin modification enzymes, have unanticipated effects on pre-tRNA end processing. Thus, this genome-wide screen uncovered putative novel pathways for tRNA nuclear export and extensive links between tRNA biology and other aspects of cell physiology.

    View details for DOI 10.1101/gad.269803.115

    View details for Web of Science ID 000366758700009

    View details for PubMedID 26680305

    View details for PubMedCentralID PMC4699390

  • Healing for destruction: tRNA intron degradation in yeast is a two-step cytoplasmic process catalyzed by tRNA ligase Rlg1 and 5 '-to-3 ' exonuclease Xrn1 GENES & DEVELOPMENT Wu, J., Hopper, A. K. 2014; 28 (14): 1556-1561

    Abstract

    In eukaryotes and archaea, tRNA splicing generates free intron molecules. Although ∼ 600,000 introns are produced per generation in yeast, they are barely detectable in cells, indicating efficient turnover of introns. Through a genome-wide search for genes involved in tRNA biology in yeast, we uncovered the mechanism for intron turnover. This process requires healing of the 5' termini of linear introns by the tRNA ligase Rlg1 and destruction by the cytoplasmic tRNA quality control 5'-to-3' exonuclease Xrn1, which has specificity for RNAs with 5' monophosphate.

    View details for DOI 10.1101/gad.244673.114

    View details for Web of Science ID 000339166100005

    View details for PubMedID 25030695

    View details for PubMedCentralID PMC4102763

  • A rapid and sensitive non-radioactive method applicable for genome-wide analysis of Saccharomyces cerevisiae genes involved in small RNA biology YEAST Wu, J., Huang, H., Hopper, A. K. 2013; 30 (4): 119-128

    Abstract

    Conventional isolation and detection methods for small RNAs from yeast cells have been designed for a limited number of samples. In order to be able to conduct a genome-wide assessment of how each gene product impacts upon small RNAs, we developed a rapid method for analysing small RNAs from Saccharomyces cerevisiae wild-type (wt) and mutants cells in the deletion and temperature-sensitive (ts) collections. Our method implements three optimized techniques: a procedure for growing small yeast cultures in 96-deepwell plates, a fast procedure for small RNA isolation from the plates, and a sensitive non-radioactive northern method for RNA detection. The RNA isolation procedure requires only 4 h for processing 96 samples, is highly reproducible and yields RNA of good quality and quantity. The non-radioactive northern method employs digoxigenin (DIG)-labelled DNA probes and chemiluminescence. It detects femtomole levels of small RNAs within 1 min exposure time. We minimized the processing time for large-scale analysis and optimized the stripping and reprobing procedures for analyses of multiple RNAs from a single membrane. The method described is rapid, sensitive, safe and cost-effective for genome-wide screens of novel genes involved in the biogenesis, subcellular trafficking and stability of small RNAs. Moreover, it will be useful to educational laboratory class venues and to research institutions with limited access to radioisotopes or robots.

    View details for DOI 10.1002/yea.2947

    View details for Web of Science ID 000317691400001

    View details for PubMedID 23417998

    View details for PubMedCentralID PMC3668450