Honors & Awards


  • University of Oregon College of Arts and Sciences Dissertation Research Fellowship, University of Oregon (07/01/17-06/30/18)
  • University of Oregon Developmental Biology Training Grant, National Institute of Health (07/01/13–06/30/16)

Professional Education


  • Doctor of Philosophy, University of Oregon (2018)
  • Major in Biology, University of Wisconsin-Milwaukee (2009)
  • Bachelor of Fine Arts, University of Utah (2006)

Stanford Advisors


Lab Affiliations


All Publications


  • TU-Tagging: A Method for Identifying Layer-Enriched Neuronal Genes in Developing Mouse Visual Cortex ENEURO Tomorsky, J., DeBlander, L., Kentros, C. G., Doe, C. Q., Niell, C. M. 2017; 4 (5)

    Abstract

    Thiouracil (TU)-tagging is an intersectional method for covalently labeling newly transcribed RNAs within specific cell types. Cell type specificity is generated through targeted transgenic expression of the enzyme uracil phosphoribosyl transferase (UPRT); temporal specificity is generated through a pulse of the modified uracil analog 4TU. This technique has been applied in mouse using a Cre-dependent UPRT transgene, CA>GFPstop>HA-UPRT, to profile RNAs in endothelial cells, but it remained untested whether 4TU can cross the blood-brain barrier (BBB) or whether this transgene can be used to purify neuronal RNAs. Here, we crossed the CA>GFPstop>HA-UPRT transgenic mouse to a Sepw1-cre line to express UPRT in layer 2/3 of visual cortex or to an Nr5a1-cre line to express UPRT in layer 4 of visual cortex. We purified thiol-tagged mRNA from both genotypes at postnatal day (P)12, as well as from wild-type (WT) mice not expressing UPRT (background control). We found that a comparison of Sepw1-purified RNA to WT or Nr5a1-purified RNA allowed us to identify genes enriched in layer 2/3 of visual cortex. Here, we show that Cre-dependent UPRT expression can be used to purify cell type-specific mRNA from the intact mouse brain and provide the first evidence that 4TU can cross the BBB to label RNA in vivo.

    View details for DOI 10.1523/ENEURO.0181-17.2017

    View details for Web of Science ID 000419519800015

    View details for PubMedID 29085897

    View details for PubMedCentralID PMC5659240