Diplom, Eberhard Karls Universitat Tubingen (2008)
Doctor of Philosophy, Eberhard Karls Universitat Tubingen (2015)
James Ferrell, Postdoctoral Faculty Sponsor
Cell-Cycle Regulation of Dynamic Chromosome Association of the Condensin Complex
2018; 23 (8): 2308–17
Eukaryotic cells inherit their genomes in the form of chromosomes, which are formed from the compaction of interphase chromatin by the condensin complex. Condensin is a member of the structural maintenance of chromosomes (SMC) family of ATPases, large ring-shaped protein assemblies that entrap DNA to establish chromosomal interactions. Here, we use the budding yeast Saccharomyces cerevisiae to dissect the role of the condensin ATPase and its relationship with cell-cycle-regulated chromosome binding dynamics. ATP hydrolysis-deficient condensin binds to chromosomes but is defective in chromosome condensation and segregation. By modulating the ATPase, we demonstrate that it controls condensin's dynamic turnover on chromosomes. Mitosis-specific phosphorylation of condensin's Smc4 subunit reduces the turnover rate. However, reducing turnover by itself is insufficient to compact chromosomes. We propose that condensation requires fine-tuned dynamic condensin interactions with more than one DNA. These results enhance our molecular understanding of condensin function during chromosome condensation.
View details for DOI 10.1016/j.celrep.2018.04.082
View details for Web of Science ID 000433052200010
View details for PubMedID 29791843
View details for PubMedCentralID PMC5986713
The Temporal Ordering of Cell-Cycle Phosphorylation.
2017; 65 (3): 371-373
Cell-cycle phosphorylation is temporally ordered, at least in part, through the sequential expression of different cyclins. Recent studies by Swaffer et al. (2016) and Godfrey et al. (2017) show that intrinsic properties of the substrate proteins contribute as well: good kinase substrates tend to be phosphorylated early, and good phosphatase substrates tend to be phosphorylated late.
View details for DOI 10.1016/j.molcel.2017.01.025
View details for PubMedID 28157499
Time To Split Up: Dynamics of Chromosome Separation
TRENDS IN CELL BIOLOGY
2017; 27 (1): 42-54
The separation of chromosomes in anaphase is a precarious step in the cell cycle. The separation is irreversible, and any error can lead to cell death or genetic instability. Chromosome separation is controlled by the protease separase. Here we discuss recent work that has revealed additional layers of separase regulation and has deepened our understanding of how separase activation is coordinated with other events of mitotic exit.
View details for DOI 10.1016/j.tcb.2016.07.008
View details for Web of Science ID 000392352800004
View details for PubMedID 27567180