Lab Affiliations

All Publications

  • INO80 Chromatin Remodeling Coordinates Metabolic Homeostasis with Cell Division CELL REPORTS Gowans, G. J., Schep, A. N., Wong, K., King, D. A., Greenleaf, W. J., Morrison, A. J. 2018; 22 (3): 611–23


    Adaptive survival requires the coordination of nutrient availability with expenditure of cellular resources. For example, in nutrient-limited environments, 50% of all S. cerevisiae genes synchronize and exhibit periodic bursts of expression in coordination with respiration and cell division in the yeast metabolic cycle (YMC). Despite the importance of metabolic and proliferative synchrony, the majority of YMC regulators are currently unknown. Here, we demonstrate that the INO80 chromatin-remodeling complex is required to coordinate respiration and cell division with periodic gene expression. Specifically, INO80 mutants have severe defects in oxygen consumption and promiscuous cell division that is no longer coupled with metabolic status. In mutant cells, chromatin accessibility of periodic genes, including TORC1-responsive genes, is relatively static, concomitant with severely attenuated gene expression. Collectively, these results reveal that the INO80 complex mediates metabolic signaling to chromatin to restrict proliferation to metabolically optimal states.

    View details for PubMedID 29346761

  • The INO80 chromatin remodeler sustains metabolic stability by promoting TOR signaling and regulating histone acetylation PLoS Genetics Beckwith, S. L., Schwartz, E. K., Garcia-Nieto, P. E., King, D. A., Gowans, G. J., Wong, K., Eckley, T. L., Paraschuk, A. P., Peltan, E. L., Lee, L. R., Yao, W., Morrison, A. J. 2018: e1007216


    Chromatin remodeling complexes are essential for gene expression programs that coordinate cell function with metabolic status. However, how these remodelers are integrated in metabolic stability pathways is not well known. Here, we report an expansive genetic screen with chromatin remodelers and metabolic regulators in Saccharomyces cerevisiae. We found that, unlike the SWR1 remodeler, the INO80 chromatin remodeling complex is composed of multiple distinct functional subunit modules. We identified a strikingly divergent genetic signature for the Ies6 subunit module that links the INO80 complex to metabolic homeostasis. In particular, mitochondrial maintenance is disrupted in ies6 mutants. INO80 is also needed to communicate TORC1-mediated signaling to chromatin, as ino80 mutants exhibit defective transcriptional profiles and altered histone acetylation of TORC1-responsive genes. Furthermore, comparative analysis reveals subunits of INO80 and mTORC1 have high co-occurrence of alterations in human cancers. Collectively, these results demonstrate that the INO80 complex is a central component of metabolic homeostasis that influences histone acetylation and may contribute to disease when disrupted.

    View details for DOI 10.1371/journal.pgen.1007216

    View details for PubMedCentralID PMC5834206

  • Methods to Promote Germination of Dormant Setaria viridis Seeds PLOS ONE Sebastian, J., Wong, M. K., Tang, E., Dinneny, J. R. 2014; 9 (4)


    Setaria viridis has recently emerged as a promising genetic model system to study diverse aspects of monocot biology. While the post-germination life cycle of S. viridis is approximately 8 weeks long, the prolonged dormancy of freshly harvested seeds can more than double the total time required between successive generations. Here we describe methods that promote seed germination in S. viridis. Our results demonstrate that treating S. viridis seeds with liquid smoke or a GA3 and KNO3 solution improves germination rates to 90% or higher even in seeds that are 6 days post-harvest with similar results obtained whether seeds are planted in soil or on gel-based media. Importantly, we show that these treatments have no significant effect on the growth of the adult plant. We have tested these treatments on diverse S. viridis accessions and show variation in their response. The methods described here will help advance research using this model grass species by increasing the pace at which successive generations of plants can be analyzed.

    View details for DOI 10.1371/journal.pone.0095109

    View details for Web of Science ID 000335226500058

    View details for PubMedID 24748008

    View details for PubMedCentralID PMC3991590