Ph.D., National University of Singapore, Fluorescent Biomaterials (2011)
M. S., Fudan University, Polymer Physics and Chemistry (2007)
B. E., East China University of Science and Technology, Materials Physics (2004)
Jianghong Rao, Postdoctoral Faculty Sponsor
Semiconducting polymer nanoprobe for in vivo imaging of reactive oxygen and nitrogen species.
Angewandte Chemie (International ed. in English)
2013; 52 (39): 10325-10329
Semiconducting polymer nanoparticles are used as a free-radical inert and light-harvesting nanoplatform for in vivo molecular imaging of reactive oxygen and nitrogen species (RONS). This nanoprobe permits detection of RONS in the microenvironment of spontaneous bacterial infection (see picture; FRET=fluorescence resonance energy transfer).
View details for DOI 10.1002/anie.201303420
View details for PubMedID 23943508
Conjugated Oligoelectrolyte Harnessed Polyhedral Oligomeric Silsesquioxane as Light-Up Hybrid Nanodot for Two-Photon Fluorescence Imaging of Cellular Nucleus
2010; 22 (37): 4186-?
A water-soluble organic/inorganic hybrid nanodot based on polyhedral oligomeric silsesquioxane (POSS) and conjugated oligoelectrolyte is designed and synthesized for two-photon fluorescence imaging of cellular nucleus, which takes advantage of its small size (?3.3 nm) that imparts nucleus permeability and substantial DNA-enhanced two-photon excited fluorescence that allows illuminating the nucleus with a high contrast.
View details for DOI 10.1002/adma.201001544
View details for Web of Science ID 000283392000013
View details for PubMedID 20589775
- Cationic Oligofluorene-Substituted Polyhedral Oligomeric Silsesquioxane as Light-Harvesting Unimolecular Nanoparticle for Fluorescence Amplification in Cellular Imaging ADVANCED MATERIALS 2010; 22 (5): 643-?
- A Graphene-Conjugated Oligomer Hybrid Probe for Light-Up Sensing of Lectin and Escherichia Coli ADVANCED MATERIALS 2011; 23 (38): 4386-?
Affibody-Attached Hyperbranched Conjugated Polyelectrolyte for Targeted Fluorescence Imaging of HER2-Positive Cancer Cell
2011; 12 (8): 2966-2974
A hyperbranched conjugated polyelectrolyte (HCPE) with a core-shell structure is designed and synthesized via alkyne polycyclotrimerization and click chemistry. The HCPE has an emission maximum at 565 nm with a quantum yield of 12% and a large Stokes shift of 143 nm in water. By virtue of its poly(ethylene glycol) shell, this polymer naturally forms spherical nanoparticles that minimize nonspecific interaction with biomolecules in aqueous solution, consequently allowing for efficient bioconjugation with anti-HER2 affibody via carbodiimide-activated coupling reaction. The resulting affibody-attached HCPE can be utilized as a reliable fluorescent probe for targeted cellular imaging of HER2-overexpressed cancer cells such as SKBR-3. Considering its low cytotoxicity and good photostability, the HCPE nanoprobe holds great promise in practical imaging tasks. This study also provides a molecular engineering strategy to overcome the intrinsic limitations of traditional fluorescent polymers (e.g., chromophore-tethered polymers and linear conjugated polyelectrolytes) for bioconjugation and applications.
View details for DOI 10.1021/bm200563a
View details for Web of Science ID 000293488200014
View details for PubMedID 21710990
Conjugated polyelectrolyte blend as perturbable energy donor-acceptor assembly with multicolor fluorescence response to proteins
2010; 46 (9): 1470-1472
Blending conjugated polyelectrolytes is demonstrated to be a convenient yet effective method to create a perturbable energy transfer systems with multicolor fluorescence response toward both nonmetalloproteins and metalloproteins, which holds great promise in visual protein sensing.
View details for DOI 10.1039/b915984c
View details for Web of Science ID 000274580700025
View details for PubMedID 20162151
- A molecular brush approach to enhance quantum yield and suppress nonspecific interactions of conjugated polyelectrolyte for far-red/near-infrared fluorescence targeted cell imaging Advanced Functional Materials 2010; 20: 2770
- Fluorescent single-molecular core-shell nanospheres of hyperbranched conjugated polyelectrolyte for live-cell imaging Chemistry of Materials 2009; 21: 3816
- Intercalating dye harnessed cationic conjugated polymer for real-time naked-eye recognition of double-stranded DNA in serum Advanced Functional Materials 2009; 19: 1371
- Conjugated polyelectrolytes as light-up macromolecular probes for heparin sensing Advanced Functional Materilas 2009; 19: 277
Optimizing the cationic conjugated polymer-sensitized fluorescent signal of dye labeled oligonucleotide for biosensor applications
BIOSENSORS & BIOELECTRONICS
2009; 24 (5): 1067-1073
Methods for real time, highly selective and sensitive polynucleotide detection are of vast scientific and economic importance. Fluorescence resonance energy transfer (FRET)-based assays which take advantage of the collective response of water-soluble conjugated polymers (CPs) and the self-assembly characteristic of aqueous polyelectrolytes have been widely used for the detection of DNA, RNA, protein and small molecules. The detection sensitivity of CP-based biosensor is dependent on the signal amplification of dye emission upon excitation of CP relative to that upon direct excitation of the dye. Using cationic polyfluorene derivatives and chromophore (fluorescein or Texas Red) labeled single-stranded DNA molecules (ssDNA-C*) as donor/acceptor pairs, we show that in addition to the spectral overlap, orientation and distance between the donor and the acceptor, the energy levels and fluorescence quenching of the donor/acceptor within the polymer/DNA-C* complexes are also important factors that affect the signal output of dye emission.
View details for DOI 10.1016/j.bios.2008.07.029
View details for Web of Science ID 000263199800002
View details for PubMedID 18760913
- Effect of charge density on energy-transfer properties of cationic conjugated polymers Advanced Functional Materials 2008; 18: 1321
- A multicolor cationic conjugated polymer for naked-eye detection and quantification of heparin Macromolecules 2008; 41: 6636