Honors & Awards


  • PhD Prize of the LMU Munich, Munich University Society (2016)
  • Römer Prize for PhD thesis, Römer Foundation (2016)
  • Römer Prize for master's thesis, Römer Foundation (2014)
  • CeNS Publication Award, Center for Nanoscience Munich (2012)
  • Scholarship, German Academic Scholarship Foundation (2009)

Professional Education


  • Doctor of Philosophy, Ludwig Maximilian Universitat Munchen (2015)
  • Master of Science, Ludwig Maximilian Universitat Munchen (2013)
  • Bachelor of Science, Ludwig Maximilian Universitat Munchen (2011)
  • Abitur, Kaiser Heinrich High School Bamberg (2008)

Stanford Advisors


Current Research and Scholarly Interests


Superresolution Microscopy, Glycobiology, Metabolic Labeling

Lab Affiliations


All Publications


  • The Endothelial Glycocalyx Controls Interactions of Quantum Dots with the Endothelium and Their Translocation across the Blood–Tissue Border ACS Nano Uhl, B., Hirn, S., Immler, R., Mildner, K., Moeckl, L., Sperandio, M., Braeuchle, C., Reichel, C., Zeuschner, D., Krombach, F. 2017; 11 (2): 1498

    View details for DOI 10.1021/acsnano.6b06812

  • Dendrimer-based signal amplification of click-labelled DNA in situ ChemBioChem Nada, R., Samuele, S., Leonhard, M., Bastien, V., Florian, G., Hanna, E., Christoph, B., Thomas, C. 2017

    View details for DOI 10.1002/cbic.201700209

  • New insights into the intracellular distribution pattern of cationic amphiphilic drugs Scientific Reports Vater, M., Moeckl, L., Gormanns, V., Schultz Fademrecht, C., Mallmann, A. M., Ziegart-Sadowska, K., Zaba, M., Frevert, M. L., Braeuchle, C., Holsboer, F., Rein, T., Schmidt, U., Kirmeier, T. 2017; 7

    View details for DOI 10.1038/srep44277

  • The glycocalyx regulates the uptake of nanoparticles by human endothelial cells in vitro Nanomedicine Moeckl, L., Hirn, S., Torrano, A., Uhl, B., Braeuchle, C., Krombach, F. 2017; 12 (3): 207-217

    View details for DOI 10.2217/nnm-2016-0332

  • Azido pentoses: A New Tool to Efficiently Label Mycobacterium tuberculosis Clinical Isolates ChemBioChem Kolbe, K., Moeckl, L., Sohst, V., Brandenburg, V., Engel, R., Malm, S., Braeuchle, C., Holst, O., Lindhorst, T., Reiling, N. 2017
  • More Than 50 Years after Its Discovery in SiO2 Octahedral Coordination Has Also Been Established in SiS2 at High Pressure Inorganic Chemistry Evers, J., Moeckl, L., Oehlinger, G., Koeppe, R., Schnoeckel, H., Barkalov, O., Medvedev, S., Naumov, P. 2017; 56 (1): 372-377
  • En route from artificial to natural: Evaluation of inhibitors of mannose-specific adhesion of E. coli under flow. Biochimica et biophysica acta Möckl, L., Fessele, C., Despras, G., Bräuchle, C., Lindhorst, T. K. 2016; 1860 (9): 2031-2036

    Abstract

    We investigated the properties of six Escherichia coli adhesion inhibitors under static and under flow conditions. On mannan-covered model substrates and under static conditions, all inhibitors were able to almost completely abolish lectin-mediated E. coli adhesion. On a monolayer of living human microvascular endothelial cells (HMEC-1), the inhibitors reduced adhesion under static conditions as well, but a large fraction of bacteria still managed to adhere even at highest inhibitor concentrations. In contrast, under flow conditions E. coli did not exhibit any adhesion to HMEC-1 not even at inhibitor concentrations where significant adhesion was detected under static conditions. This indicates that the presence of shear stress strongly affects inhibitor properties and must be taken into account when evaluating the potency of bacterial adhesion inhibitors.

    View details for DOI 10.1016/j.bbagen.2016.06.021

    View details for PubMedID 27345501

  • Artificial Formation and Tuning of Glycoprotein Networks on Live Cell Membranes: A Single-Molecule Tracking Study CHEMPHYSCHEM Moeckl, L., Lindhorst, T. K., Braeuchle, C. 2016; 17 (6): 829-835
  • 100 Jahre Einkristallzucht aus der Schmelze - Vom Spreeknie ins Silicon Valley CHEMIE IN UNSERER ZEIT Evers, J., Herzog, C., Moeckl, L., von Plotho, C., Stallhofer, P., Staudigl, R. 2016; 50 (6)

    View details for DOI 10.1002/ciuz.201600759

  • Switching first contact: photocontrol of E. coli adhesion to human cells CHEMICAL COMMUNICATIONS Moeckl, L., Mueller, A., Braeuchle, C., Lindhorst, T. K. 2016; 52 (6): 1254-1257

    Abstract

    We have shown previously that carbohydrate-specific bacterial adhesion to a non-physiological surface can be photocontrolled by reversible E/Z isomerisation using azobenzene-functionalised sugars. Here, this approach is applied to the surface of human cells. We show not only that bacterial adhesion to the azobenzene glycoside-modified cell surface is higher in the E than in the Z state, but add data about the specific modulation of the effect.

    View details for DOI 10.1039/c5cc08884d

    View details for Web of Science ID 000368353600037

    View details for PubMedID 26612767

  • Microdomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycoproteins CHEMBIOCHEM Moeckl, L., Horst, A. K., Kolbe, K., Lindhorst, T. K., Braeuchle, C. 2015; 16 (14): 2023-2028

    Abstract

    The effect of galectin-mediated microdomain formation on the spatiotemporal dynamics of glycosylated membrane proteins in human microvascular endothelial cells (HMEC-1) was studied qualitatively and quantitatively by high-resolution fluorescence microscopy and artificially mimicked by metabolic glycoprotein engineering. Two types of membrane proteins, sialic acid-bearing proteins (SABPs) and mucin-type proteins (MTPs), were investigated. For visualization they were metabolically labeled with azido sugars and then coupled to a cyclooctyne-conjugated fluorescent dye by click chemistry. Both spatial (diffusion) and temporal (residence time) dynamics of SABPs and MTPs on the membrane were investigated after treatment with exogenous galectin-1 or -3. Strong effects of galectin-mediated lattice formation were observed for MTPs (decreased spatial mobility), but not for SABPs. Lattice formation also strongly decreased the turnover of MTPs (increased residence time on the cell membrane). The effects of galectin-mediated crosslinking was accurately mimicked by streptavidin-mediated crosslinking of biotin-tagged glycoproteins and verified by single-molecule tracking. This technique allows the induction of crosslinking of membrane proteins under precisely controlled conditions, thereby influencing membrane residence time and the spatial dynamics of glycans on the cell membrane in a controlled way.

    View details for DOI 10.1002/cbic.201500361

    View details for Web of Science ID 000362815900009

    View details for PubMedID 26296625

  • With logical Sharpness and systematic Inflexibility Wichard von Moellendorff CHEMIE IN UNSERER ZEIT Evers, J., Moeckl, L. 2015; 49 (4): 236-247
  • Two High-Pressure Phases of SiS2 as Missing Links between the Extremes of Only Edge-Sharing and Only Corner-Sharing Tetrahedra INORGANIC CHEMISTRY Evers, J., Mayer, P., Moeckl, L., Oehlinger, G., Koeppe, R., Schnoeckel, H. 2015; 54 (4): 1240-1253

    Abstract

    The ambient pressure phase of silicon disulfide (NP-SiS2), published in 1935, is orthorhombic and contains chains of distorted, edge-sharing SiS4 tetrahedra. The first high pressure phase, HP3-SiS2, published in 1965 and quenchable to ambient conditions, is tetragonal and contains distorted corner-sharing SiS4 tetrahedra. Here, we report on the crystal structures of two monoclinic phases, HP1-SiS2 and HP2-SiS2, which can be considered as missing links between the orthorhombic and the tetragonal phase. Both monoclinic phases contain edge- as well as corner-sharing SiS4 tetrahedra. With increasing pressure, the volume contraction (-ΔV/V) and the density, compared to the orthorhombic NP-phase, increase from only edge-sharing tetrahedra to only corner-sharing tetrahedra. The lattice and the positional parameters of NP-SiS2, HP1-SiS2, HP2-SiS2, and HP3-SiS2 were derived in good agreement with the experimental data from group-subgroup relationships with the CaF2 structure as aristotype. In addition, the Raman spectra of SiS2 show that the most intense bands of the new phases HP1-SiS2 and HP2-SiS2 (408 and 404 cm(-1), respectively) lie between those of NP-SiS2 (434 cm(-1)) and HP3-SiS2 (324 cm(-1)). Density functional theory (DFT) calculations confirm these observations.

    View details for DOI 10.1021/ic501825r

    View details for Web of Science ID 000349656600015

    View details for PubMedID 25590815

  • Cell-Penetrating and Neurotargeting Dendritic siRNA Nanostructures ANGEWANDTE CHEMIE-INTERNATIONAL EDITION Brunner, K., Harder, J., Halbach, T., Willibald, J., Spada, F., Gnerlich, F., Sparrer, K., Beil, A., Moeckl, L., Braeuchle, C., Conzelmann, K., Carell, T. 2015; 54 (6): 1946-1949

    Abstract

    We report the development of dendritic siRNA nanostructures that are able to penetrate even difficult to transfect cells such as neurons with the help of a special receptor ligand. The nanoparticles elicit strong siRNA responses, despite the dendritic structure. An siRNA dendrimer directed against the crucial rabies virus (RABV) nucleoprotein (N protein) and phosphoprotein (P protein) allowed the suppression of the virus titer in neurons below the detection limit. The cell-penetrating siRNA dendrimers, which were assembled using click chemistry, open up new avenues toward finding novel molecules able to cure this deadly disease.

    View details for DOI 10.1002/anie.201409803

    View details for Web of Science ID 000349209200052

    View details for PubMedID 25522332

  • Super-resolved Fluorescence Microscopy: Nobel Prize in Chemistry 2014 for Eric Betzig, Stefan Hell, and William E. Moerner ANGEWANDTE CHEMIE-INTERNATIONAL EDITION Moeckl, L., Lamb, D. C., Braeuchle, C. 2014; 53 (51): 13972-13977

    Abstract

    A big honor for small objects: The Nobel Prize in Chemistry 2014 was jointly awarded to Eric Betzig, Stefan Hell, and William E. Moerner "for the development of super-resolved fluorescence microscopy". This Highlight describes how the field of super-resolution microscopy developed from the first detection of a single molecule in 1989 to the sophisticated techniques of today.

    View details for DOI 10.1002/anie.201410265

    View details for Web of Science ID 000346484400001

    View details for PubMedID 25371081

  • Optical Investigations to clear up a Mystery The Wittelsbach and the Hope Diamond CHEMIE IN UNSERER ZEIT Evers, J., Moeckl, L., Noeth, H. 2012; 46 (6): 356-364
  • Tuning Nanoparticle Uptake: Live-Cell Imaging Reveals Two Distinct Endocytosis Mechanisms Mediated by Natural and Artificial EGFR Targeting Ligand NANO LETTERS Mickler, F. M., Moeckl, L., Ruthardt, N., Ogris, M., Wagner, E., Braeuchle, C. 2012; 12 (7): 3417-3423

    Abstract

    Therapeutic nanoparticles can be directed to cancer cells by incorporating selective targeting ligands. Here, we investigate the epidermal growth factor receptor (EGFR)-mediated endocytosis of gene carriers (polyplexes) either targeted with natural EGF or GE11, a short synthetic EGFR-binding peptide. Highly sensitive live-cell fluorescence microcopy with single particle resolution unraveled the existence of two different uptake mechanisms; EGF triggers accelerated nanoparticle endocytosis due to its dual active role in receptor binding and signaling activation. For GE11, an alternative EGFR signaling independent, actin-driven pathway is presented.

    View details for DOI 10.1021/nl300395q

    View details for Web of Science ID 000306296200011

    View details for PubMedID 22632479