Mahla Poudineh received her B.Sc. and M.Sc. in Electrical Engineering, both from the University of Tehran, Iran in 2010 and 2012, respectively. She then completed a Ph.D. degree in Electrical Engineering (with Biomedical Engineering focus) at the University of Toronto in 2016. Before joining Stanford as a postdoctoral fellow, she completed a one-year postdoctoral training at the University of Toronto, Department of Pharmaceutical Science. Her research interests include design and implementation of micro- and nano-fabrication techniques for biomedical engineering applications such as phenotypic profiling of rare cancer cells and real-time monitoring for infectious disease diagnosis.
Ph.D., University of Toronto, Electrical Engineering (2016)
M.Sc., University of Tehran, Electrical Engineering (2012)
B.Sc., University of Tehran, Electrical Engineering (2010)
Three-Dimensional Nanostructured Architectures Enable Efficient Neural Differentiation of Mesenchymal Stem Cells via Mechanotransduction
2018; 18 (11): 7188–93
Cell morphology and geometry affect cellular processes such as stem cell differentiation, suggesting that these parameters serve as fundamental regulators of biological processes within the cell. Hierarchical architectures featuring micro- and nanotopographical features therefore offer programmable systems for stem cell differentiation. However, a limited number of studies have explored the effects of hierarchical architectures due to the complexity of fabricating systems with rationally tunable micro- and nanostructuring. Here, we report three-dimensional (3D) nanostructured microarchitectures that efficiently regulate the fate of human mesenchymal stem cells (hMSCs). These nanostructured architectures strongly promote cell alignment and efficient neurogenic differentiation where over 85% of hMSCs express microtubule-associated protein 2 (MAP2), a mature neural marker, after 7 days of culture on the nanostructured surface. Remarkably, we found that the surface morphology of nanostructured surface is a key factor that promotes neurogenesis and that highly spiky structures promote more efficient neuronal differentiation. Immunostaining and gene expression profiling revealed significant upregulation of neuronal markers compared to unpatterned surfaces. These findings suggest that the 3D nanostructured microarchitectures can play a critical role in defining stem cell behavior.
View details for DOI 10.1021/acs.nanolett.8b03313
View details for Web of Science ID 000451102100071
View details for PubMedID 30335391