All Publications

  • Split Green Fluorescent Proteins: Scope, Limitations, and Outlook. Annual review of biophysics Romei, M. G., Boxer, S. G. 2019


    Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies. Expected final online publication date for the Annual Review of Biophysics Volume 48 is May 3, 2019. Please see for revised estimates.

    View details for PubMedID 30786230

  • Photoactive Split Green Fluorescent Protein: Engineering a New Optogenetic and Imaging System Romei, M. G., Longwell, C. K., Cochran, J. R., Boxer, S. G. CELL PRESS. 2018: 177A–178A