Professional Education


  • Bachelor of Science, Lebanese American University (2013)
  • Doctor of Philosophy, Universite De Provence (Aix Marseille) (2018)
  • Master of Science, Lebanese American University (2015)

Stanford Advisors


All Publications


  • Culturomics, a potential approach paving the way towardbacteriotherapy. Current opinion in microbiology Matar, G., Bilen, M. 2022; 69: 102194

    Abstract

    The human microbiota has been extensively studied over the past decade to describe its role in health and diseases. Numerous studies showed the presence of bacterial imbalance in a variety of human health conditions, suggesting great potential for the development of bacteriotherapies. Identifying mechanisms involving the human microbiota has been very challenging due to the complex data generated by molecular approaches and the limited number of organisms isolated by culture and described. This review summarizes the efforts done to describe the human microbiota through culturomics and the advancements in culturing the organisms residing at different body sites.

    View details for DOI 10.1016/j.mib.2022.102194

    View details for PubMedID 35994842

  • Neglectibacter timonensis gen. nov., sp. nov. and Scatolibacter rhodanostii gen. nov., sp. nov., two anaerobic bacteria isolated from human stool samples. Archives of microbiology Zgheib, R., Ibrahim, A., Anani, H., Ndongo, S., Bilen, M., Armstrong, N., Richez, M., Raoult, D., Fournier, P. 1800; 204 (1): 45

    Abstract

    Strains Marseille-P2265T (=CSUR P2265T=DSM 102082T) and Marseille-P3890T (=CSUR P3890T=CCUG 72341T) were isolated from stool samples using the culturomics approach. The 16S rRNA gene sequences of both strains were sequenced and compared by BLASTn to the NCBI database. Strains Marseille-P2265T and Marseille-P3890T were most closely related to Acutalibacter muris with identities of 94.3% and 91.5%, respectively. Between the two strains, the 16S rRNA gene sequence identity was 91.5%. Both strains are anaerobic Gram-positive, oxidase- and catalase-negative. The major fatty acid methyl esters (>10%) in both strains are C16:0 and anteiso-C15:0. Additionally, strain Marseille-P2265T has iso-C15:0 and C14:0, and strain Marseille-P3890T, iso-C14:0. Strain Marseille-P2265T has a genome size of 3,671,396-bp with a G+C content of 52.8%. As for strain Marseille-P3890T, the genome is 2,702,024-bp-long with a 39.8% G+C content. The genomic comparison of closely related species with strains Marseille-P2265T and Marseille-P3890T showed that all digital DNA-DNA hybridization (dDDH), orthologous average nucleotide identity (OrthoANI) and average amino acid identity (AAI) values were lower than the published species thresholds (70% for dDDH, 95-96% for OrthoANI/AAI). Based on these results, it was concluded that strains Marseille-P2265T and Marseille-P3890T belong to two new genera in the family Oscillospiraceae. For these two genera, the names Neglectibacter gen. nov. and Scatolibacter gen. nov. were proposed, with strains Marseille-P2265T and Marseille-P3890T being the type strains of Neglectibacter timonensis gen. nov., sp. nov. and Scatolibacter rhodanostii gen. nov., sp. nov., respectively.

    View details for DOI 10.1007/s00203-021-02712-w

    View details for PubMedID 34932147

  • Strategies and advancements in human microbiome description and the importance of culturomics. Microbial pathogenesis Bilen, M. n. 2020: 104460

    Abstract

    The human microbiota gained a big interest among the scientific community with numerous studies being performed to better understand its role in health and diseases. Even with all the success achieved in studying the bacterial populations at the different body sites and its interaction among each other and with the host, some links remain missing and might have therapeutic benefits. In this review, we summarize the main means used for bacterial identification, human microbiota description and the role of culturomics in leading the way towards the development of new bacterio-therapeutic approaches.

    View details for DOI 10.1016/j.micpath.2020.104460

    View details for PubMedID 32853680