Honors & Awards
NMSS Postdoctoral Fellowship, National Multiple Sclerosis Society (July 2015)
NRSA Postdoctoral Fellowship, NINDS (Jan. - June 2015)
Neonatology and Developmental Biology Postdoctoral Training Grant, Stanford / NICHD (Jan. 2014 - Dec. 2014)
NRSA Predoctoral Fellowship, NINDS (Jan. 2011 - Dec. 2013)
System and Integrative Biology Predoctoral Training Grant, UPenn / NIGMS (Sept. 2007 - Aug. 2009)
Bachelor of Science, California Institute of Technology (2006)
Doctor of Philosophy, University of Pennsylvania (2013)
Current Research and Scholarly Interests
mRNA transport and local translation in oligodendrocytes
Microtubule organization in oligodendrocytes
MAPK8IP1/JIP1 regulates the trafficking of autophagosomes in neurons.
2014; 10 (11): 2079-2081
Autophagy is a spatially regulated process in axons; autophagosomes form preferentially in the distal axon tip then move actively and processively toward the cell body. Despite the primarily unidirectional transport observed in live-cell imaging experiments, both anterograde-directed KIF5/kinesin-1 motors and retrograde-directed dynein motors are tightly associated with axonal autophagosomes. Here, we discuss our recent work identifying the scaffolding protein MAPK8IP1/JIP1 (mitogen-activated protein kinase 8 interacting protein 1) as a key regulator of autophagosome transport in neurons. MAPK8IP1 tightly coordinates motor activity to ensure the fidelity of retrograde autophagosome transport in the axon.
View details for DOI 10.4161/auto.34451
View details for PubMedID 25483967
Integrated regulation of motor-driven organelle transport by scaffolding proteins
TRENDS IN CELL BIOLOGY
2014; 24 (10): 564-574
Intracellular trafficking pathways, including endocytosis, autophagy, and secretion, rely on directed organelle transport driven by the opposing microtubule motor proteins kinesin and dynein. Precise spatial and temporal targeting of vesicles and organelles requires the integrated regulation of these opposing motors, which are often bound simultaneously to the same cargo. Recent progress demonstrates that organelle-associated scaffolding proteins, including Milton/TRAKs (trafficking kinesin-binding protein), JIP1, JIP3 (JNK-interacting proteins), huntingtin, and Hook1, interact with molecular motors to coordinate activity and sustain unidirectional transport. Scaffolding proteins also bind to upstream regulatory proteins, including kinases and GTPases, to modulate transport in the cell. This integration of regulatory control with motor activity allows for cargo-specific changes in the transport or targeting of organelles in response to cues from the complex cellular environment.
View details for DOI 10.1016/j.tcb.2014.05.002
View details for Web of Science ID 000343630000003
View details for PubMedID 24953741
LC3 Binding to the Scaffolding Protein JIP1 Regulates Processive Dynein-Driven Transport of Autophagosomes
2014; 29 (5): 577-590
Autophagy is essential for maintaining cellular homeostasis in neurons, where autophagosomes undergo robust unidirectional retrograde transport along axons. We find that the motor scaffolding protein JIP1 binds directly to the autophagosome adaptor LC3 via a conserved LIR motif. This interaction is required for the initial exit of autophagosomes from the distal axon, for sustained retrograde transport along the midaxon, and for autophagosomal maturation in the proximal axon. JIP1 binds directly to the dynein activator dynactin but also binds to and activates kinesin-1 in a phosphorylation-dependent manner. Following JIP1 depletion, phosphodeficient JIP1-S421A rescues retrograde transport, while phosphomimetic JIP1-S421D aberrantly activates anterograde transport. During normal autophagosome transport, residue S421 of JIP1 may be maintained in a dephosphorylated state by autophagosome-associated MKP1 phosphatase. Moreover, binding of LC3 to JIP1 competitively disrupts JIP1-mediated activation of kinesin. Thus, dual mechanisms prevent aberrant activation of kinesin to ensure robust retrograde transport of autophagosomes along the axon.
View details for DOI 10.1016/j.devcel.2014.04.015
View details for Web of Science ID 000337644700010
View details for PubMedID 24914561
JIP1 regulates the directionality of APP axonal transport by coordinating kinesin and dynein motors
JOURNAL OF CELL BIOLOGY
2013; 202 (3): 495-508
Regulation of the opposing kinesin and dynein motors that drive axonal transport is essential to maintain neuronal homeostasis. Here, we examine coordination of motor activity by the scaffolding protein JNK-interacting protein 1 (JIP1), which we find is required for long-range anterograde and retrograde amyloid precursor protein (APP) motility in axons. We identify novel interactions between JIP1 and kinesin heavy chain (KHC) that relieve KHC autoinhibition, activating motor function in single molecule assays. The direct binding of the dynactin subunit p150(Glued) to JIP1 competitively inhibits KHC activation in vitro and disrupts the transport of APP in neurons. Together, these experiments support a model whereby JIP1 coordinates APP transport by switching between anterograde and retrograde motile complexes. We find that mutations in the JNK-dependent phosphorylation site S421 in JIP1 alter both KHC activation in vitro and the directionality of APP transport in neurons. Thus phosphorylation of S421 of JIP1 serves as a molecular switch to regulate the direction of APP transport in neurons.
View details for DOI 10.1083/jcb.201302078
View details for Web of Science ID 000322769400011
View details for PubMedID 23897889
Retrograde axonal transport: pathways to cell death?
TRENDS IN NEUROSCIENCES
2010; 33 (7): 335-344
Active transport along the axon is crucial to the neuron. Motor-driven transport supplies the distal synapse with newly synthesized proteins and lipids, and clears damaged or misfolded proteins. Microtubule motors also drive long-distance signaling along the axon via signaling endosomes. Although positive signaling initiated by neurotrophic factors has been well-studied, recent research has focused on stress-signaling along the axon. Here, the connections between axonal transport alterations and neurodegeneration are discussed, including evidence for defective transport of vesicles, mitochondria, degradative organelles, and signaling endosomes in models of amyotrophic lateral sclerosis, Huntington's, Parkinson's and Alzheimer's disease. Defects in transport are sufficient to induce neurodegeneration, but recent progress suggests that changes in retrograde signaling pathways correlate with rapidly progressive neuronal cell death.
View details for DOI 10.1016/j.tins.2010.03.006
View details for Web of Science ID 000280279900005
View details for PubMedID 20434225