Professional Education

  • Bachelor of Science, University of Science and Technology of China (2007)
  • Doctor of Philosophy, University of Southern California (2014)

Stanford Advisors

All Publications

  • Scaling down of balanced excitation and inhibition by active behavioral states in auditory cortex NATURE NEUROSCIENCE Zhou, M., Liang, F., Xiong, X. R., Li, L., Li, H., Xiao, Z., Tao, H. W., Zhang, L. I. 2014; 17 (6): 841-850


    Cortical sensory processing is modulated by behavioral and cognitive states. How this modulation is achieved by changing synaptic circuits remains largely unknown. In awake mouse auditory cortex, we found that sensory-evoked spike responses of layer 2/3 (L2/3) excitatory cells were scaled down with preserved sensory tuning when mice transitioned from quiescence to active behaviors, including locomotion, whereas L4 and thalamic responses were unchanged. Whole-cell voltage-clamp recordings revealed that tone-evoked synaptic excitation and inhibition exhibited a robust functional balance. The change to active states caused scaling down of excitation and inhibition at approximately equal levels in L2/3 cells, but resulted in no synaptic changes in L4 cells. This lamina-specific gain control could be attributed to an enhancement of L1-mediated inhibitory tone, with L2/3 parvalbumin inhibitory neurons also being suppressed. Thus, L2/3 circuits can adjust the salience of output in accordance with momentary behavioral demands while maintaining the sensitivity and quality of sensory processing.

    View details for DOI 10.1038/nn.3701

    View details for Web of Science ID 000336638000020

    View details for PubMedID 24747575

  • Synaptic Mechanisms for Generating Temporal Diversity of Auditory Representation in the Dorsal Cochlear Nucleus. Journal of neurophysiology Zhou, M., Li, Y. T., Yuan, W., Tao, H. W., Zhang, L. I. 2014: jn.00573.2014


    In central auditory pathways neurons exhibit a great diversity of temporal discharge patterns, which may contribute to the parallel processing of auditory signals. How such response diversity emerges in the central auditory circuits remains unclear. Here, we investigated whether synaptic mechanisms can contribute to the generation of the temporal response diversity at the first stage along the central auditory neuraxis. By in vivo whole-cell voltage-clamp recording in the dorsal cochlear nucleus (DCN) of rats, we revealed excitatory and inhibitory synaptic inputs underlying three different firing patterns of fusiform/pyramidal neurons in response to auditory stimuli: "primary-like", "pauser", and "buildup" patterns. We found that primary-like neurons received strong fast-rising excitation, whereas pauser and buildup neurons received accumulating excitation with a relatively weak fast rising phase followed by a slow rising phase. Pauser neurons received stronger fast-rising excitation than buildup cells. On the other hand, inhibitory inputs to the three types of cell exhibited similar temporal patterns all with a strong fast-rising phase. Dynamic-clamp recordings demonstrated that the differential temporal patterns of excitation could primarily account for the different discharge patterns. In addition, discharge pattern in a single neuron varied in a stimulus-dependent manner, which could be attributed to the modulation of excitation/inhibition balance by different stimuli. Further examination of excitatory inputs to vertical/tuberculoventral and cartwheel cells suggested that fast-rising and accumulating excitation might be conveyed by auditory nerve and parallel fibers respectively. A differential summation of excitatory inputs from the two sources may thus contribute to the generation of response diversity.

    View details for DOI 10.1152/jn.00573.2014

    View details for PubMedID 25475349

  • Intracortical multiplication of thalamocortical signals in mouse auditory cortex NATURE NEUROSCIENCE Li, L., Li, Y., Zhou, M., Tao, H. W., Zhang, L. I. 2013; 16 (9): 1179-U25


    Cortical processing of sensory information begins with the transformation of thalamically relayed signals. We optogenetically silenced intracortical circuits to isolate thalamic inputs to layer 4 neurons and found that intracortical excitation linearly amplified thalamocortical responses underlying frequency and direction selectivity, with spectral range and tuning preserved, and prolonged the response duration. This signal pre-amplification and prolongation enhanced the salience of thalamocortically relayed information and ensured its robust, faithful and more persistent representation.

    View details for DOI 10.1038/nn.3493

    View details for Web of Science ID 000323597500006

    View details for PubMedID 23933752

  • Generation of Intensity Selectivity by Differential Synaptic Tuning: Fast-Saturating Excitation But Slow-Saturating Inhibition JOURNAL OF NEUROSCIENCE Zhou, M., Tao, H. W., Zhang, L. I. 2012; 32 (50): 18068-18078


    Intensity defines one fundamental aspect of sensory information and is specifically represented in each sensory modality. Interestingly, only in the central auditory system are intensity-selective neurons evolved. These neurons are characterized by nonmonotonic response-level functions. The synaptic circuitry mechanisms underlying the generation of intensity selectivity from nonselective auditory nerve inputs remain largely unclear. Here, we performed in vivo whole-cell recordings from pyramidal neurons in the rat dorsal cochlear nucleus (DCN), where intensity selectivity first emerges along the auditory neuraxis. Our results revealed that intensity-selective cells received fast-saturating excitation but slow-saturating inhibition with intensity increments, whereas in intensity-nonselective cells excitation and inhibition were similarly slow-saturating. The differential intensity tuning profiles of the monotonic excitation and inhibition qualitatively determined the intensity selectivity of output responses. In addition, the selectivity was further strengthened by significantly lower excitation/inhibition ratios at high-intensity levels compared with intensity-nonselective neurons. Our results demonstrate that intensity selectivity in the DCN is generated by extracting the difference between tuning profiles of nonselective excitatory and inhibitory inputs, which we propose can be achieved through a differential circuit mediated by feedforward inhibition.

    View details for DOI 10.1523/JNEUROSCI.3647-12.2012

    View details for Web of Science ID 000312404700014

    View details for PubMedID 23238722

  • Fine-tuning of pre-balanced excitation and inhibition during auditory cortical development NATURE Sun, Y. J., Wu, G. K., Liu, B., Li, P., Zhou, M., Xiao, Z., Tao, H. W., Zhang, L. I. 2010; 465 (7300): 927-U8


    Functional receptive fields of neurons in sensory cortices undergo progressive refinement during development. Such refinement may be attributed to the pruning of non-optimal excitatory inputs, reshaping of the excitatory tuning profile through modifying the strengths of individual inputs, or strengthening of cortical inhibition. These models have not been directly tested because of the technical difficulties in assaying the spatiotemporal patterns of functional synaptic inputs during development. Here we apply in vivo whole-cell voltage-clamp recordings to the recipient layer 4 neurons in the rat primary auditory cortex (A1) to determine the developmental changes in the frequency-intensity tonal receptive fields (TRFs) of their excitatory and inhibitory inputs. Surprisingly, we observe co-tuned excitation and inhibition immediately after the onset of hearing, suggesting that a tripartite thalamocortical circuit with relatively strong feedforward inhibition is formed independently of auditory experience. The frequency ranges of tone-driven excitatory and inhibitory inputs first expand within a few days of the onset of hearing and then persist into adulthood. The latter phase is accompanied by a sharpening of the excitatory but not inhibitory frequency tuning profile, which results in relatively broader inhibitory tuning in adult A1 neurons. Thus the development of cortical synaptic TRFs after the onset of hearing is marked by a slight breakdown of previously formed excitation-inhibition balance. Our results suggest that functional refinement of cortical TRFs does not require a selective pruning of inputs, but may depend more on a fine adjustment of excitatory input strengths.

    View details for DOI 10.1038/nature09079

    View details for Web of Science ID 000278804500038

    View details for PubMedID 20559386