Bachelor's degree, Seoul National University, Chemistry (2006)
Doctor of Philosophy, Seoul National University (2014)
Michael Lin, Postdoctoral Research Mentor
Fluorescent indicators for simultaneous reporting of all four cell cycle phases.
A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
View details for DOI 10.1038/nmeth.4045
View details for PubMedID 27798610
Direct and precise length measurement of single, stretched DNA fragments by dynamic molecular combing and STED nanoscopy
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2016; 408 (23): 6453-6459
A combination of DNA stretching method and super-resolution nanoscopy allows an accurate and precise measurement of the length of DNA fragments ranging widely in size from 117 to 23,130 bp. BstEII- and HindIII-treated λDNA fragments were stained with an intercalating dye and then linearly stretched on a coverslip by dynamic molecular combing. The image of individual DNA fragments was obtained by stimulated emission depletion nanoscopy. For DNA fragments longer than ∼1000 bp, the measured lengths of DNA fragments were consistently within ∼0.5 to 1.0 % of the reference values, raising the possibility of this method in a wide range of applications including facile detection for copy number variations and trinucleotide repeat disorder.
View details for DOI 10.1007/s00216-016-9764-9
View details for Web of Science ID 000382681900022
View details for PubMedID 27457103
- Photoelectron Spectroscopy of 4-Bromochlorobenzene Dimer and Trimer Anions BULLETIN OF THE KOREAN CHEMICAL SOCIETY 2013; 34 (9): 2565-2566
- Associative Electron Attachment to 2-Aminopyridine-(CO2)(1) Complex: Photoelectron Spectroscopic and Theoretical Approach BULLETIN OF THE KOREAN CHEMICAL SOCIETY 2013; 34 (8): 2247-2248
- Anion Photoelectron Spectroscopy and Theoretical Calculation of the Hetero-dimers of Polycyclic Aromatic Hydrocarbons BULLETIN OF THE KOREAN CHEMICAL SOCIETY 2013; 34 (5): 1441-1444
Atomic selectivity in dissociative electron attachment to dihalobenzenes
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
2013; 15 (39): 16503-16509
We investigated electron attachment to three dihalobenzene molecules, bromochlorobenzene (BCB), bromoiodobenzene (BIB) and chloroiodobenzene (CIB), by molecular beam photoelectron spectroscopy. The most prominent product of electron attachment in the anion mass spectra was the atomic fragment of the less electronegative halogen of the two, i.e., Br(-) for BCB and I(-) for BIB and CIB. Photoelectron spectroscopy and ab initio calculations suggested that the approaching electron prefers to attack the less electronegative atom, a seemingly counterintuitive finding but consistent with the mass spectrometric result. For the iodine-containing species BIB and CIB, the photoelectron spectrum consists of bands from both the molecular anion and atomic I(-), the latter of which is produced by photodissociation of the former. Molecular orbital analysis revealed that a large degree of orbital energy reordering takes place upon electron attachment. These phenomena were shown to be readily explained by simple molecular orbital theory and the electronegativity of the halogen atoms.
View details for DOI 10.1039/c3cp50456e
View details for Web of Science ID 000324749900016
View details for PubMedID 23936900
- Electron affinity of phenanthrene and ion core structure of its anion clusters RSC ADVANCES 2013; 3 (38): 17143-17149
- Rapid and facile synthesis of a (ZnxAgyInz)S-2 nanocrystal library via sono-combichem method and its characterization including single nanocrystal analysis JOURNAL OF MATERIALS CHEMISTRY 2012; 22 (24): 11957-11963
"Associative" Electron Attachment to Azabenzene-(CO2)(n) van der Waals Complexes: Stepwise Formation of Covalent Bonds with Additive Electron Affinities
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2008; 130 (48): 16241-16244
Electron attachment to the van der Waals complexes of azabenzene-(CO(2))(n) results in the formation of covalent bonds between the nitrogen atoms of azabenzene and the carbon atom of CO(2). The newly formed C-N bonds establish an extended pi-orbital network over the entire moieties of the complex and thus greatly stabilize the anion, yielding a very large value of vertical detachment energy for the excess electron. The rare "associative" nature of electron attachment was found to be generally occurring in all azabenzene series, only subject to steric hindrance against the formation of the covalent C-N bond.
View details for DOI 10.1021/ja8039103
View details for Web of Science ID 000263319800038
View details for PubMedID 18998649