Professional Education


  • Doctor of Philosophy, University of Southern California, Pharmaceutical Sciences (Olenyuk Lab) (2012)
  • Graduate Student, University of Arizona (2005-2010), Chemistry and Biochemistry (Olenyuk Lab)
  • Master of Science, Panjab University (2004)

Lab Affiliations


All Publications


  • Small-molecule-directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Maruotti, J., Sripathi, S. R., Bharti, K., Fuller, J., Wahlin, K. J., Ranganathan, V., Sluch, V. M., Berlinicke, C. A., Davis, J., Kim, C., Zhao, L., Wan, J., Qian, J., Corneo, B., Temple, S., Dubey, R., Olenyuk, B. Z., Bhutto, I., Lutty, G. A., Zack, D. J. 2015; 112 (35): 10950-10955

    Abstract

    Age-related macular degeneration (AMD) is associated with dysfunction and death of retinal pigment epithelial (RPE) cells. Cell-based approaches using RPE-like cells derived from human pluripotent stem cells (hPSCs) are being developed for AMD treatment. However, most efficient RPE differentiation protocols rely on complex, stepwise treatments and addition of growth factors, whereas small-molecule-only approaches developed to date display reduced yields. To identify new compounds that promote RPE differentiation, we developed and performed a high-throughput quantitative PCR screen complemented by a novel orthogonal human induced pluripotent stem cell (hiPSC)-based RPE reporter assay. Chetomin, an inhibitor of hypoxia-inducible factors, was found to strongly increase RPE differentiation; combination with nicotinamide resulted in conversion of over one-half of the differentiating cells into RPE. Single passage of the whole culture yielded a highly pure hPSC-RPE cell population that displayed many of the morphological, molecular, and functional characteristics of native RPE.

    View details for DOI 10.1073/pnas.1422818112

    View details for Web of Science ID 000360383200049

    View details for PubMedID 26269569