Honors & Awards


  • BioX Bowes Fellow, Stanford BioX (2014-current)
  • SPARK Award, Stanford University - SPARK Translational Research Program (2015-current)

Education & Certifications


  • Master of Science, Stanford University, BIOE-MS (2015)
  • SB, MIT, Biological Engineering (2013)

Stanford Advisors


All Publications


  • Targeted Drug Delivery with an Integrin-Binding Knottin-Fc-MMAF Conjugate Produced by Cell-Free Protein Synthesis MOLECULAR CANCER THERAPEUTICS Currier, N. V., Ackerman, S. E., Kintzing, J. R., Chen, R., Interrante, M. F., Steiner, A., Sato, A. K., Cochran, J. R. 2016; 15 (6): 1291-1300
  • Targeted Drug Delivery with an Integrin-Binding Knottin-Fc-MMAF Conjugate Produced by Cell-Free Protein Synthesis. Molecular cancer therapeutics Currier, N. V., Ackerman, S. E., Kintzing, J. R., Chen, R., Filsinger Interrante, M., Steiner, A., Sato, A. K., Cochran, J. R. 2016; 15 (6): 1291-1300

    Abstract

    Antibody-drug conjugates (ADC) have generated significant interest as targeted therapeutics for cancer treatment, demonstrating improved clinical efficacy and safety compared with systemic chemotherapy. To extend this concept to other tumor-targeting proteins, we conjugated the tubulin inhibitor monomethyl-auristatin-F (MMAF) to 2.5F-Fc, a fusion protein composed of a human Fc domain and a cystine knot (knottin) miniprotein engineered to bind with high affinity to tumor-associated integrin receptors. The broad expression of integrins (including αvβ3, αvβ5, and α5β1) on tumor cells and their vasculature makes 2.5F-Fc an attractive tumor-targeting protein for drug delivery. We show that 2.5F-Fc can be expressed by cell-free protein synthesis, during which a non-natural amino acid was introduced into the Fc domain and subsequently used for site-specific conjugation of MMAF through a noncleavable linker. The resulting knottin-Fc-drug conjugate (KFDC), termed 2.5F-Fc-MMAF, had approximately 2 drugs attached per KFDC. 2.5F-Fc-MMAF inhibited proliferation in human glioblastoma (U87MG), ovarian (A2780), and breast (MB-468) cancer cells to a greater extent than 2.5F-Fc or MMAF alone or added in combination. As a single agent, 2.5F-Fc-MMAF was effective at inducing regression and prolonged survival in U87MG tumor xenograft models when administered at 10 mg/kg two times per week. In comparison, tumors treated with 2.5F-Fc or MMAF were nonresponsive, and treatment with a nontargeted control, CTRL-Fc-MMAF, showed a modest but not significant therapeutic effect. These studies provide proof-of-concept for further development of KFDCs as alternatives to ADCs for tumor targeting and drug delivery applications. Mol Cancer Ther; 15(6); 1291-300. ©2016 AACR.

    View details for DOI 10.1158/1535-7163.MCT-15-0881

    View details for PubMedID 27197305

  • Cystine-knot peptides: emerging tools for cancer imaging and therapy EXPERT REVIEW OF PROTEOMICS Ackerman, S. E., Currier, N. V., Bergen, J. M., Cochran, J. R. 2014; 11 (5): 561-572
  • A Bioengineered Peptide that Localizes to and Illuminates Medulloblastoma: A New Tool with Potential for Fluorescence-Guided Surgical Resection Cureus Ackerman, S. E., Wilson, C. M., Kahn, S. A., Kintzing, J. R., Jindal, D. A., Cheshier, S. H., Grant, G. A., Cochran, J. R. 2014

    View details for DOI 10.7759/cureus.207