Shreya Deshmukh

All Publications

• Multiparametric biophysical profiling of red blood cells in malaria infection. Communications biology Deshmukh, S. S., Shakya, B., Chen, A., Durmus, N. G., Greenhouse, B., Egan, E. S., Demirci, U. 2021; 4 (1): 697

  Abstract

  Biophysical separation promises label-free, less-invasive methods to manipulate the diverse properties of live cells, such as density, magnetic susceptibility, and morphological characteristics. However, some cellular changes are so minute that they are undetectable by current methods. We developed a multiparametric cell-separation approach to profile cells with simultaneously changing density and magnetic susceptibility. We demonstrated this approach with the natural biophysical phenomenon of Plasmodium falciparum infection, which modifies its host erythrocyte by simultaneously decreasing density and increasing magnetic susceptibility. Current approaches have used these properties separately to isolate later-stage infected cells, but not in combination. We present biophysical separation of infected erythrocytes by balancing gravitational and magnetic forces to differentiate infected cell stages, including early stages for the first time, using magnetic levitation. We quantified height distributions of erythrocyte populations-27 ring-stage synchronized samples and 35 uninfected controls-and quantified their unique biophysical signatures. This platform can thus enable multidimensional biophysical measurements on unique cell types.

  View details for DOI 10.1038/s42003-021-02181-3

  View details for PubMedID 34103669

• Reversible Design of Dynamic Assemblies at Small Scales ADVANCED INTELLIGENT SYSTEMS Soto, F., Wang, J., Deshmukh, S., Demirci, U. 2021; 3 (4)

  View details for DOI 10.1002/aisy.202000193

  View details for Web of Science ID 000669806800008

• A confirmatory test for sperm in sexual assault samples using a microfluidic-integrated cell phone imaging system. Forensic science international. Genetics Deshmukh, S., Inci, F., Karaaslan, M. G., Ogut, M. G., Duncan, D., Klevan, L., Duncan, G., Demirci, U. 2020; 48: 102313

  Abstract

  Rapid and efficient processing of sexual assault evidence to accelerate forensic investigation and decrease casework backlogs is urgently needed. Therefore, the standardized protocols currently used in forensic laboratories can benefit from continued innovation to handle the increasing number and complexity of samples being submitted to forensic labs. To our knowledge, there is currently no available rapid and portable forensic screening technology based on a confirmatory test for sperm identification in a sexual assault kit. Here, we present a novel forensic sample screening tool, i.e., a microchip integrated with a portable cell phone imaging platform that records and processes images for further investigation and storage. The platform (i) precisely and rapidly screens swab samples (<15 min after sample preparation on-chip); (ii) selectively captures sperm from mock sexual assault samples using a novel and previously published SLeX-based surface chemistry treatment (iii) separates non-sperm contents (epithelial cells and debris in this case) out of the channel by flow prior to imaging; (iv) captures cell phone images on a portable cellphone-integrated imaging platform, (v) quantitatively differentiates sperm cells from epithelial cells, using a morphology detection code that leverages Laplacian of Gaussian and Hough gradient transform methods; (vi) is sensitive within a forensic cut-off (>95% accuracy) compared to the manual counts; (vii) provides a cost-effective and timely solution to a problem which in the past has taken a great deal of time; and (viii) handles small volumes of sample (20 muL). This integration of the cellphone imaging platform and cell recognition algorithms with disposable microchips can be a new direction toward a direct visual test to screen and differentiate sperm from epithelial cell types in forensic samples for a crime laboratory scenario. With further development, this integrated platform could assist a sexual assault nurse examiner (SANE) in a hospital or sexual assault treatment center facility to flag sperm-containing samples prior to further downstream testing.

  View details for DOI 10.1016/j.fsigen.2020.102313

  View details for PubMedID 32570000

• MICROSCALE MAGNETIC LEVITATION FOR MULTIPLEXED ANALYSIS OF MALARIA-INFECTED BLOOD SAMPLES IN RESOURCE-LIMITED SETTINGS Deshmukh, S. S., Durmus, N., Greenhouse, B., Egan, E., Demirci, U. AMER SOC TROP MED & HYGIENE. 2019: 130–31

  View details for Web of Science ID 000507364502427