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  • An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism SCIENTIFIC REPORTS Shimizu, Y., Bandaru, S., Hara, M., Young, S., Sano, T., Usami, K., Kurano, Y., Lee, S., Kumagai-Takei, N., Takashiba, S., Sano, S., Ito, T. 2023; 13 (1): 10413


    We herein elucidate the function of SARS-CoV-2derived 5'UTR in the human cells. 5'UTR bound host cellular RNAs were immunoprecipitated by gRNA-dCas13 (targeting luciferase RNA fused to SARS-CoV-2 5'UTR) in HEK293T and A549 cells. The 5'UTR bound RNA extractions were predominantly enriched for regulating lipid metabolism. Overexpression of SARS-CoV-2 5'UTR RNA altered the expression of factors involved in the process of the human Mevalonate pathway. In addition, we found that HMG-CoA reductase inhibitors were shown to suppress SARS-CoV-2 5'UTR-mediated translation activities. In conclusion, we deduce the array of host RNAs interacting with SARS-CoV-2 5'UTR that drives SARS-CoV-2 translation and influences host metabolic pathways.

    View details for DOI 10.1038/s41598-023-36680-6

    View details for Web of Science ID 001088220200013

    View details for PubMedID 37369697

    View details for PubMedCentralID PMC10300121