Honors & Awards
Research Fellowship, German Research Foundation (2013-)
Doctor of Philosophy, Universitat Regensburg (2012)
Diplom, Universitat Regensburg (2008)
Vordiplom, Universitat Regensburg (2006)
Karlene Cimprich, Postdoctoral Faculty Sponsor
Conflict Resolution in the Genome: How Transcription and Replication Make It Work
2016; 167 (6): 1455-1467
The complex machineries involved in replication and transcription translocate along the same DNA template, often in opposing directions and at different rates. These processes routinely interfere with each other in prokaryotes, and mounting evidence now suggests that RNA polymerase complexes also encounter replication forks in higher eukaryotes. Indeed, cells rely on numerous mechanisms to avoid, tolerate, and resolve such transcription-replication conflicts, and the absence of these mechanisms can lead to catastrophic effects on genome stability and cell viability. In this article, we review the cellular responses to transcription-replication conflicts and highlight how these inevitable encounters shape the genome and impact diverse cellular processes.
View details for DOI 10.1016/j.cell.2016.09.053
View details for Web of Science ID 000389470500010
View details for PubMedID 27912056
View details for PubMedCentralID PMC5141617
The contribution of co-transcriptional RNA:DNA hybrid structures to DNA damage and genome instability.
2014; 19: 84-94
Accurate DNA replication and DNA repair are crucial for the maintenance of genome stability, and it is generally accepted that failure of these processes is a major source of DNA damage in cells. Intriguingly, recent evidence suggests that DNA damage is more likely to occur at genomic loci with high transcriptional activity. Furthermore, loss of certain RNA processing factors in eukaryotic cells is associated with increased formation of co-transcriptional RNA:DNA hybrid structures known as R-loops, resulting in double-strand breaks (DSBs) and DNA damage. However, the molecular mechanisms by which R-loop structures ultimately lead to DNA breaks and genome instability is not well understood. In this review, we summarize the current knowledge about the formation, recognition and processing of RNA:DNA hybrids, and discuss possible mechanisms by which these structures contribute to DNA damage and genome instability in the cell.
View details for DOI 10.1016/j.dnarep.2014.03.023
View details for PubMedID 24746923