Master of Science, University of Michigan Ann Arbor (2017)
Doctor of Philosophy, University of Michigan Ann Arbor (2017)
- In situelectrochemical H(2)production for efficient and stable power-to-gas electromethanogenesis GREEN CHEMISTRY 2020; 22 (18): 6194–6203
Enhanced Electrosynthetic Hydrogen Evolution by Hydrogenases Embedded in a Redox-Active Hydrogel.
Chemistry (Weinheim an der Bergstrasse, Germany)
Molecular hydrogen is a major high-energy carrier for future energy technologies, if produced from renewable electrical energy. Hydrogenase enzymes offer a pathway for bioelectrochemically producing hydrogen that is advantageous over traditional platforms for hydrogen production because of low overpotentials and ambient operating temperature and pressure. However, electron delivery from the electrode surface to the enzyme's active site is often rate-limiting. Here, we show three different hydrogenases from Clostridium pasteurianum and Methanococcus maripaludis , when immobilized at a cathode in a cobaltocene-functionalized polyallylamine (Cc-PAA) redox polymer, mediate rapid and efficient hydrogen evolution. We further show that Cc-PAA-mediated hydrogenases can operate at high faradaic efficiency (80-100%) and low overpotential (-0.578 to -0.593 V vs. SHE). Specific activities of these hydrogenases in the electrosynthetic Cc-PAA assay were comparable to their respective activities in traditional methyl viologen assays, indicating that Cc-PAA mediates electron transfer at high rates, to most of the embedded enzymes.
View details for DOI 10.1002/chem.202000750
View details for PubMedID 32074397