Stanford Advisors

  • Wah Chiu, Postdoctoral Faculty Sponsor

Contact

  • Academic
    dflesher@stanford.edu
    University - Scholar Department: Bioengineering Position: Postdoctoral Scholar

Additional Info

All Publications

  • High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, <i>Synechocystis</i> sp. PCC 6803 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Gisriel, C. J., Wang, J., Liu, J., Flesher, D. A., Reiss, K. M., Huang, H., Yang, K. R., Armstrong, W. H., Gunner, M. R., Batista, V. S., Debus, R. J., Brudvig, G. W. 2022; 119 (1)

    Abstract

    Photosystem II (PSII) enables global-scale, light-driven water oxidation. Genetic manipulation of PSII from the mesophilic cyanobacterium Synechocystis sp. PCC 6803 has provided insights into the mechanism of water oxidation; however, the lack of a high-resolution structure of oxygen-evolving PSII from this organism has limited the interpretation of biophysical data to models based on structures of thermophilic cyanobacterial PSII. Here, we report the cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 at 1.93-Å resolution. A number of differences are observed relative to thermophilic PSII structures, including the following: the extrinsic subunit PsbQ is maintained, the C terminus of the D1 subunit is flexible, some waters near the active site are partially occupied, and differences in the PsbV subunit block the Large (O1) water channel. These features strongly influence the structural picture of PSII, especially as it pertains to the mechanism of water oxidation.

    View details for DOI 10.1073/pnas.2116765118

    View details for Web of Science ID 000748065500010

    View details for PubMedID 34937700

    View details for PubMedCentralID PMC8740770

https://orcid.org/0000-0002-8270-4751