All Publications

  • Early life stress moderates the relation between systemic inflammation and neural activation to reward in adolescents both cross-sectionally and longitudinally. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology Yuan, J. P., Coury, S. M., Ho, T. C., Gotlib, I. H. 2023


    Elevated levels of systemic inflammation are associated with altered reward-related brain function in ventral striatal areas of the brain like the nucleus accumbens (NAcc). In adolescents, cross-sectional research indicates that exposure to early life stress (ELS) can moderate the relation between inflammation and neural activation, which may contribute to atypical reward function; however, no studies have tested whether this moderation by ELS of neuroimmune associations persists over time. Here, we conducted a cross-sectional analysis and the first exploratory longitudinal analysis testing whether cumulative severity of ELS moderates the association of systemic inflammation with reward-related processing in the NAcc in adolescents (n = 104; 58F/46M; M[SD] age = 16.00[1.45] years; range = 13.07-19.86 years). For the cross-sectional analysis, we modeled a statistical interaction between ELS and levels of C-reactive protein (CRP) predicting NAcc activation during the anticipation and outcome phases of a monetary reward task. We found that higher CRP was associated with blunted NAcc activation during the outcome of reward in youth who experienced higher levels of ELS (β = -0.31; p = 0.006). For the longitudinal analysis, we modeled an interaction between ELS and change in CRP predicting change in NAcc activation across 2 years. This analysis similarly showed that increasing CRP over time was associated with decreasing NAcc during reward outcomes in youth who experienced higher levels of ELS (β = -0.47; p = 0.022). Both findings support contemporary theoretical frameworks involving associations among inflammation, reward-related brain function, and ELS exposure, and suggest that experiencing ELS can have significant and enduring effects on neuroimmune function and adolescent neurodevelopment.

    View details for DOI 10.1038/s41386-023-01708-y

    View details for PubMedID 37673968

    View details for PubMedCentralID 7147972

  • Early life stress, systemic inflammation, and neural correlates of implicit emotion regulation in adolescents. Brain, behavior, and immunity Yuan, J. P., Ho, T. C., Coury, S. M., Chahal, R., Colich, N. L., Gotlib, I. H. 2022


    Exposure to early life stress (ELS) increases the risk for developing psychopathology; however, the mechanisms underlying this association are not clear. In this study we examined systemic inflammation as a pathway that may link exposure to stress to altered neural correlates of implicit emotion regulation in adolescents with varying levels of exposure to ELS (n=83; 52 females, 31 males; 15.63±1.10 years). We measured ventrolateral prefrontal cortex (vlPFC) activation and functional connectivity (FC) between the bilateral amygdala and the vlPFC as adolescents completed an affect labeling task in the scanner and assessed concentrations of C-reactive protein (CRP) using a dried blood spot protocol. We found that CRP levels were negatively associated with vlPFC activation during implicit regulation of negatively-valenced stimuli, and that cumulative severity of ELS exposure moderated this neuroimmune association. Severity of ELS also significantly moderated the association between CRP levels and FC between the bilateral amygdala and L-vlPFC during implicit emotion regulation: in adolescents who had been exposed to more severe ELS, higher CRP was associated with more negative frontoamygdala FC during implicit regulation of negatively-valenced stimuli. Thus, ELS may disrupt the normative association between the immune system and the neural processes that underlie socioemotional functioning potentially increasing adolescents' risk for maladaptive outcomes.

    View details for DOI 10.1016/j.bbi.2022.07.007

    View details for PubMedID 35842188

  • Trajectories of Depressive Symptoms and Reward Circuitry in Adolescence Following Early Life Stress: A Longitudinal Assessment Borchers, L., Yuan, J., Chahal, R., Ryu, J., Colich, N., Gotlib, I. ELSEVIER SCIENCE INC. 2022: S79
  • Dimensions of Early Adversity and the Development of Functional Brain Network Connectivity During Adolescence: Implications for Trajectories of Internalizing Symptoms Chahal, R., Miller, J. G., Yuan, J. P., Buthmann, J. L., Ho, T. C., Gotlib, I. H. ELSEVIER SCIENCE INC. 2022: S48
  • An exploration of dimensions of early adversity and the development of functional brain network connectivity during adolescence: Implications for trajectories of internalizing symptoms. Development and psychopathology Chahal, R., Miller, J. G., Yuan, J. P., Buthmann, J. L., Gotlib, I. H. 1800: 1-15


    Different dimensions of adversity may affect mental health through distinct neurobiological mechanisms, though current supporting evidence consists largely of cross-sectional associations between threat or deprivation and fronto-limbic circuitry. In this exploratory three-wave longitudinal study spanning ages 9-19 years, we examined the associations between experiences of unpredictability, threat, and deprivation with the development of functional connectivity within and between three brain networks implicated in psychopathology: the salience (SAL), default mode (DMN), and fronto-parietal (FPN) networks, and tested whether network trajectories moderated associations between adversity and changes in internalizing symptoms. Connectivity decreased with age on average; these changes differed by dimension of adversity. Whereas family-level deprivation was associated with lower initial levels and more stability across most networks, unpredictability was associated with stability only in SAL connectivity, and threat was associated with stability in FPN and DMN-SAL connectivity. In youth exposed to higher levels of any adversity, lower initial levels and more stability in connectivity were related to smaller increases in internalizing symptoms. Our findings suggest that whereas deprivation is associated with widespread neurodevelopmental differences in cognitive and emotion processing networks, unpredictability is related selectively to salience detection circuitry. Studies with wider developmental windows should examine whether these neurodevelopmental alterations are adaptive or serve to maintain internalizing symptoms.

    View details for DOI 10.1017/S0954579421001814

    View details for PubMedID 35094729

  • Reduced anxiety and changes in amygdala network properties in adolescents with training for awareness, resilience, and action (TARA). NeuroImage. Clinical Tymofiyeva, O., Henje, E., Yuan, J. P., Huang, C., Connolly, C. G., Ho, T. C., Bhandari, S., Parks, K. C., Sipes, B. S., Yang, T. T., Xu, D. 2020; 29: 102521


    Mindfulness-based approaches show promise to improve emotional health in youth and may help treat and prevent adolescent depression and anxiety. However, there is a fundamental gap in understanding the neural reorganization that takes place as a result of such interventions. The Training for Awareness, Resilience, and Action (TARA) program, initially developed for depressed adolescents, uses a framework drawn from neuroscience, mindfulness, yoga, and modern psychotherapeutic techniques to promote emotional health. The goal of this study was to assess the effects of the TARA training on emotional health and structural white matter brain networks in healthy youth. We analyzed data from 23 adolescents who underwent the 12-week TARA training in a controlled within-subject study design and whose brain networks were assessed using diffusion MRI connectomics. Compared to the control time period, adolescents showed a significant decrease in anxiety symptoms with TARA (Cohen's d=-0.961, p=0.006); moreover, the node strength of the Right Amygdala decreased significantly after TARA (Cohen's d=-1.026, p=0.004). Post-hoc analyses indicated that anxiety at baseline before TARA was positively correlated with Right Amygdala node strength (r=0.672, p=0.001). While change in Right Amygdala node strength with TARA was not correlated with change in anxiety (r=0.146, p=0.51), it was associated with change in depression subscale of Anhedonia / Negative Affect (r=0.575, p=0.004, exploratory analysis), possibly due to overlapping constructs captured in our anxiety and depression scales. Our results suggest that increased structural connectivity of Right Amygdala may underlie increased anxiety in adolescents and be lowered through anxiety-reducing training such as TARA. The results of this study contribute to our understanding of the neural mechanisms of TARA and may facilitate neuroscience-based prevention and treatment of adolescent anxiety and depression.

    View details for DOI 10.1016/j.nicl.2020.102521

    View details for PubMedID 33316764

  • Neural Correlates of Smartphone Dependence in Adolescents FRONTIERS IN HUMAN NEUROSCIENCE Tymofiyeva, O., Yuan, J. P., Kidambi, R., Huang, C., Henje, E., Rubinstein, M. L., Jariwala, N., Max, J. E., Yang, T. T., Xu, D. 2020; 14: 564629


    Increases in depressive and suicide-related symptoms among United States adolescents have been recently linked to increased use of smartphones. Understanding of the brain mechanisms that underlie the potential smartphone dependence may help develop interventions to address this important problem. In this exploratory study, we investigated the neural mechanisms underlying potential smartphone dependence in a sample of 19 adolescent volunteers who completed self-assessments of their smartphone dependence, depressive symptoms, and sleep problems. All 19 adolescents underwent diffusion MRI that allowed for assessment of white matter structural connectivity within the framework of connectomics. Based on previous literature on the neurobiology of addiction, we hypothesized a disruption of network centrality of three nodes in the mesolimbic network: Nucleus Accumbens, anterior cingulate cortex, and amygdala. Our results showed positive correlations between the node centrality of the right amygdala and self-reported smartphone dependence, between smartphone dependence and sleep problems, and between sleep problems and depressive symptoms. A higher phone dependence was observed in females compared to males. Supported by these results, we propose a model of how smartphone dependence can be linked to aberrations in brain networks, sex, sleep disturbances, and depression in adolescents.

    View details for DOI 10.3389/fnhum.2020.564629

    View details for Web of Science ID 000580387000001

    View details for PubMedID 33132878

    View details for PubMedCentralID PMC7577047

  • Rate of radiation-induced microbleed formation on 7T MRI relates to cognitive impairment in young patients treated with radiation therapy for a brain tumor. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology Morrison, M. A., Mueller, S., Felton, E., Jakary, A., Stoller, S., Avadiappan, S., Yuan, J., Molinaro, A. M., Braunstein, S., Banerjee, A., Hess, C. P., Lupo, J. M. 2020; 154: 145-153


    Radiation therapy (RT) is essential to the management of many brain tumors, but has been known to lead to cognitive decline and vascular injury in the form of cerebral microbleeds (CMBs).In a subset of children, adolescents, and young adults recruited from a larger trial investigating arteriopathy and stroke risk after RT, we evaluated the prevalence of CMBs after RT, examined risk factors for CMBs and cognitive impairment, and related their longitudinal development to cognitive performance changes.Twenty-five patients (mean 17 years, range: 10-25 years) underwent 7-Tesla MRI and cognitive assessment. Nineteen patients were treated with whole-brain or focal RT 1-month to 20-years prior, while 6 non-irradiated patients with posterior-fossa tumors served as controls. CMBs were detected on 7T susceptibility-weighted imaging (SWI) using semi-automated software, a first use in this population.CMB detection sensitivity with 7T SWI was higher than previously reported at lower field strengths, with one or more CMBs detected in 100% of patients treated with RT at least 1-year prior. CMBs were localized to dose-targeted brain volumes with risk factors including whole-brain RT (p = 0.05), a higher RT dose (p = 0.01), increasing time since RT (p = 0.03), and younger age during RT (p = 0.01). Apart from RT dose, these factors were associated with impaired memory performance. Follow-up data in a subset of patients revealed a proportional increase in CMB count with worsening verbal memory performance (r = -0.85, p = 0.03).Treatment with RT during youth is associated with the chronic development of CMBs that evolve with memory impairment over time.

    View details for DOI 10.1016/j.radonc.2020.09.028

    View details for PubMedID 32966846

  • Gray Matter Changes in Adolescents Participating in a Meditation Training. Frontiers in human neuroscience Yuan, J. P., Connolly, C. G., Henje, E., Sugrue, L. P., Yang, T. T., Xu, D., Tymofiyeva, O. 2020; 14: 319


    Meditation has shown to benefit a wide range of conditions and symptoms, but the neural mechanisms underlying the practice remain unclear. Magnetic resonance imaging (MRI) studies have investigated the structural brain changes due to the practice by examining volume, density, or cortical thickness changes. However, these studies have focused on adults; meditation's structural effects on the adolescent brain remain understudied. In this study, we investigated how meditation training affects the structure of the adolescent brain by scanning a group of 38 adolescents (16.48 ± 1.29 years) before and after participating in a 12-week meditation training. Subjects underwent Training for Awareness, Resilience, and Action (TARA), a program that mainly incorporates elements from mindfulness meditation and yoga-based practices. A subset of the adolescents also received an additional control scan 12 weeks before TARA. We conducted voxel-based morphometry (VBM) to assess gray matter volume changes pre- to post-training and during the control period. Subjects showed significant gray matter (GM) volume decreases in the left posterior insula and to a lesser extent in the left thalamus and left putamen after meditation training. There were no significant changes during the control period. Our results support previous findings that meditation affects regions associated with physical and emotional awareness. However, our results are different from previous morphometric studies in which meditation was associated with structural increases. We posit that this discrepancy may be due to the differences between the adolescent brain and the adult brain.

    View details for DOI 10.3389/fnhum.2020.00319

    View details for PubMedID 32922278

  • Test-Retest Reliability of Graph Theoretic Metrics in Adolescent Brains BRAIN CONNECTIVITY Yuan, J. P., Blom, E., Flynn, T., Chen, Y., Ho, T. C., Connolly, C. G., Walter, R., Yang, T. T., Xu, D., Tymofiyeva, O. 2019; 9 (2): 144–54
  • High levels of mitochondrial DNA are associated with adolescent brain structural hypoconnectivity and increased anxiety but not depression JOURNAL OF AFFECTIVE DISORDERS Tymofiyeva, O., Blom, E., Ho, T. C., Connolly, C. G., Lindqvist, D., Wolkowitz, O. M., Lin, J., LeWinn, K. Z., Sacchet, M. D., Han, L. M., Yuan, J. P., Bhandari, S. P., Xu, D., Yang, T. T. 2018; 232: 283–90


    Adolescent anxiety and depression are highly prevalent psychiatric disorders that are associated with altered molecular and neurocircuit profiles. Recently, increased mitochondrial DNA copy number (mtDNA-cn) has been found to be associated with several psychopathologies in adults, especially anxiety and depression. The associations between mtDNA-cn and anxiety and depression have not, however, been investigated in adolescents. Moreover, to date there have been no studies examining associations between mtDNA-cn and brain network alterations in mood disorders in any age group.The first aim of this study was to compare salivary mtDNA-cn between 49 depressed and/or anxious adolescents and 35 well-matched healthy controls. The second aim of this study was to identify neural correlates of mtDNA-cn derived from diffusion tensor imaging (DTI) and tractography, in the full sample of adolescents.There were no diagnosis-specific alterations in mtDNA-cn. However, there was a positive correlation between mtDNA-cn and levels of anxiety, but not depression, in the full sample of adolescents. A subnetwork of connections largely corresponding to the left fronto-occipital fasciculus had significantly lower fractional anisotropy (FA) values in adolescents with higher than median mtDNA-cn.Undifferentiated analysis of free and intracellular mtDNA and use of DTI-based tractography represent this study's limitations.The results of this study help elucidate the relationships between clinical symptoms, molecular changes, and neurocircuitry alterations in adolescents with and without anxiety and depression, and they suggest that increased mtDNA-cn is associated both with increased anxiety symptoms and with decreased fronto-occipital structural connectivity in this population.

    View details for PubMedID 29500956

    View details for PubMedCentralID PMC5864120