Contact

  • Academic
    shreya7@stanford.edu
    University - Student Department: Bioengineering Position: Graduate

Additional Info

  • Mail Code: 2259

All Publications

  • Development of a Novel Hybprinter-SAM for Functionally Graded Tissue Engineering Constructs with Patterned and Localized Biochemical Signals. Research square Li, J., Kim, C., Alizadeh, H. V., Garg, S., Bruyas, A., Zhao, P., Passos, I. S., Pan, C. C., Pérez, A. S., Skylar-Scott, M. A., Kim, S., Yang, Y. P. 2025

    Abstract

    Engineering native-mimetic tissue constructs is challenging due to their intricate biological and structural gradients. To address this, Hybprinter-SAM was developed by integrating three bioprinting technologies: syringe extrusion (SE), acoustic droplet ejection (ADE) and molten material extrusion (MME). This system not only enables the creation of mechanical gradients by integrating soft and rigid materials spanning 7 order magnitude of stiffness but also facilitates precise patterning and controlled localization of biochemical signals within printed scaffolds. This capability is beneficial in replicating the complexity of native tissues to enhance functionality. Both the printing process and biomaterials were optimized to balance printability, mechanical integrity, and biocompatibility. As a proof of concept, Hybprinter-SAM was used in a bone-tendon regeneration study to engineer a multi-material construct with patterned fibroblast growth factor 2 (FGF-2), resulting in markers indicative of fibrocartilage development. These findings highlight the potential of Hybprinter-SAM as a versatile platform for diverse tissue engineering applications that require complex, functionally graded tissue constructs.

    View details for DOI 10.21203/rs.3.rs-5898561/v1

    View details for PubMedID 39975888

    View details for PubMedCentralID PMC11838729