Alix Guevara Tique

All Publications

• Precision Delivery of Therapeutics to the Intestine: A Case Series of Superselective Steroid Administration in Pediatric Intestinal Transplant Recipients. Transplantation Levitte, S., Guevara-Tique, A. A., Ganguly, A., Syed, A., Gugig, R., Zhang, K. Y., Bonham, C. A., Thakor, A. S. 2024; 108 (8): e202-e203

  View details for DOI 10.1097/TP.0000000000005053

  View details for PubMedID 39042371

• Mesenchymal stem cell-derived extracellular vesicles for the treatment of acute rejection in pediatric and adult bowel transplant AMERICAN JOURNAL OF TRANSPLANTATION Levitte, S., Zhang, K., Guevara-Tique, A. A., Ganguly, A., Dulken, B. W., Nilkant, R., Rigmaiden, M., Kumari, R., Khlifi, K., Thakor, A. S., Bonham, C. A. 2024; 24 (4): 693-696

  View details for DOI 10.1016/j.ajt.2023.10.019

  View details for Web of Science ID 001217324600001

• Detection of<i> Helicobacter</i><i> pylori</i> in Adults with Dyspepsia in Ibague, Colombia: Comparative Evaluation of Methods UNIVERSITAS MEDICA Medina, A., Trujillo, P., Valencia, F., Tique, A., Olaya, J., Arciniegas, N., Parra, G., de Polanco, M. A., Bohorquez, M. 2024; 65: 1-11

  View details for DOI 10.11144/Javeriana.umed65.dhpa

  View details for Web of Science ID 001373405500001

• Mesenchymal stem cell-derived extracellular vesicles for the treatment of acute rejection in pediatric and adult bowel transplant. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons Levitte, S., Zhang, K. Y., Guevara-Tique, A. A., Ganguly, A., Dulken, B. W., Nilkant, R., Rigmaiden, M., Kumari, R., Khlifi, K., Thakor, A. S., Bonham, C. A. 2023

  Abstract

  Mesenchymal stem cells are under investigation as a novel therapy to treat solid organ transplant rejection. However, significant hurdles have limited their use in humans. Mesenchymal stem cell-derived extracellular vesicles address many of these shortcomings but have not been investigated clinically. Here we report our experience treating two patients with graft rejection and inflammation following bowel transplant using mesenchymal stem cell-derived extracellular vesicles.

  View details for DOI 10.1016/j.ajt.2023.10.019

  View details for PubMedID 39491097

• Precision Delivery of Steroids as a Rescue Therapy for Gastrointestinal Graft-versus-Host Disease in Pediatric Stem Cell Transplant Recipients Journal of Clinical Medicine Levitte, s., Ganguly, A., Frolik, S., Guevara-Tique, A., et al 2023; 12 (4229)

  View details for DOI 10.3390/jcm12134229

• Recombination events drives the emergence of Colombian Helicobacter pylori subpopulations with self-identity ancestry VIRULENCE Guevara-Tique, A. A., Torres, R. C., Bravo, M. M., Carvajal Carmona, L. G., Echeverry de Polanco, M. M., Bohorquez, M. E., Torres, J. 2022; 13 (1): 1146-1160

  Abstract

  Helicobacter pylori have coevolved with mankind since its origins, adapting to different human groups. In America, H. pylori has evolved into several subpopulations. We analysed the genome of 154 Colombian strains along with 1,091 strains from worldwide populations to discern the ancestry and adaption to Colombian people. Population structure and ancestry was inferred with FineStructure and ChromoPainter. Phylogenetic relationship and the relative effect of recombination were analysing the core SNPs. Also, a Fst index was calculated to identify the gene variants with the strongest fixation in the Colombian subpopulations compared to their parent population hspSWEurope. FineStructure allowed the identification of two Colombian subpopulations, the previously described hspSWEuropeColombia and a novel subpopulation named hspColombia, that included three subgroups following their geographic origin. Colombian subpopulations represent an admixture of European, African and Indigenous ancestry; although some genomes showed a high proportion of self identity, suggesting an advanced adaption to these mestizo Colombian groups. We found that recombination is more important that punctual mutations in H. pylori genome diversity, 13.9 more important in hspSWEurope, 12.5 in hspSWEColombia and 10.5 in hspColombia, reflecting the divergence of these subpopulations. Fst analysis identified 82 SNPs fixed in 26 genes of the hspColombia subpopulation that encode for outer membrane and central metabolism proteins. Strongest fixation indexes were identified in genes encoding HofC, HopE, FrpB-4 and Sialidase A. These findings demonstrate that H. pylori has evolved in Colombia to give rise to subpopulations with a self identity ancestry, reflected in allele changes on genes encoding for outer membrane proteins.

  View details for DOI 10.1080/21505594.2022.2095737

  View details for Web of Science ID 000825952600001

  View details for PubMedID 35838227

  View details for PubMedCentralID PMC9291697

• Multiregional Sequencing Analysis Reveals Extensive Genetic Heterogeneity in Gastric Tumors from Latinos. Cancer Research Communications Toal, T., Polanco-Echeverry, M. M., Sahasrabudhe, R., Lott, P., Suarez-Olaya, J. J., Guevara-Tique, A. A., et al 2022; 2 (11): 1496

  Abstract

  Gastric cancer is a leading cause of cancer mortality and health disparities in Latinos. We evaluated gastric intratumoral heterogeneity using multiregional sequencing of >700 cancer genes in 115 tumor biopsies from 32 patients, 29 who were Latinos. Analyses focused on comparisons with The Cancer Genome Atlas (TCGA) and on mutation clonality, druggability, and signatures. We found that only approximately 30% of all mutations were clonal and that only 61% of the known TCGA gastric cancer drivers harbored clonal mutations. Multiple clonal mutations were found in new candidate gastric cancer drivers such as EYS, FAT4, PCDHA1, RAD50, EXO1, RECQL4, and FSIP2. The genomically stable (GS) molecular subtype, which has the worse prognosis, was identified in 48% of our Latino patients, a fraction that was >2.3-fold higher than in TCGA Asian and White patients. Only a third of all tumors harbored clonal pathogenic mutations in druggable genes, with most (93%) GS tumors lacking actionable clonal mutations. Mutation signature analyses revealed that, in microsatellite-stable (MSS) tumors, DNA repair mutations were common for both tumor initiation and progression, while tobacco, POLE, and inflammation signatures likely initiate carcinogenesis. MSS tumor progression was likely driven by aging- and aflatoxin-associated mutations, as these latter changes were usually nonclonal. In microsatellite-unstable tumors, nonclonal tobacco-associated mutations were common. Our study, therefore, contributed to advancing gastric cancer molecular diagnostics and suggests clonal status is important to understanding gastric tumorigenesis. Our findings of a higher frequency of a poor prognosis associated molecular subtype in Latinos and a possible new aflatoxin gastric cancer etiology also advance cancer disparities research.Our study contributes to advancing our knowledge of gastric carcinogenesis, diagnostics, and cancer health disparities.

  View details for DOI 10.1158/2767-9764.CRC-22-0149

  View details for PubMedCentralID PMC10035402

• Molecular evaluation of Helicobacter pylori infection in 470 Colombian patients with premalignant lesion and gastric cancer Guevara-Tique, A., Suarez Olaya, J., Castro-Valencia, F., Parra Gil, G., Torres, R., Torres, J., Carvajal Carmona, L., Echeverry de Polanco, M., Bohorquez Lozano, M. ELSEVIER. 2020: S221

  View details for DOI 10.1016/j.annonc.2020.04.026

  View details for Web of Science ID 000558722000400

• Diversity of Helicobacter pylori genotypes in tumoral, antral and normal tissue of Colombian patients with gastric cancer Guevara-Tique, A., Suarez Olaya, J., Castro-Valencia, F., Torres, R., Parra Gil, G., Torres, J., Carmona, L., Echeverry de Polanco, M., Bohorquez Lozano, M. ELSEVIER. 2020: S172

  View details for DOI 10.1016/j.annonc.2020.04.333

  View details for Web of Science ID 000558722000252

• Genome Sequences of Three Colombian Helicobacter pylori Strains Isolated from Tolimense Patients MICROBIOLOGY RESOURCE ANNOUNCEMENTS Guevara, A. A., Torres, R. C., Suarez, J. J., Castro-Valencia, F. L., Parra, G., Torres, J., Carmona, L., Echeverry de Polanco, M., Bohorquez, M. E. 2020; 9 (18)

  Abstract

  We present the complete genome sequences of three Helicobacter pylori strains isolated from patients who resided in Tolima Department, Colombia, diagnosed with chronic gastritis. The genomes present an average length of 1.6 Mbp and 1,546 genes and correspond to different H. pylori subpopulations.

  View details for DOI 10.1128/MRA.00117-20

  View details for Web of Science ID 000531020500010

  View details for PubMedID 32354968

  View details for PubMedCentralID PMC7193923

• A Systematic Review of Genetic Coevolution of Homo sapiens and Helicobacter pylori: Implications for Development of Gastric Cancer Revista colombiana de Gastroenterología Guevara Tique, A. A., et al 2016; 31 (4): 376-390