William Moerner, Postdoctoral Faculty Sponsor
3D super-resolution deep-tissue imaging in living mice.
2021; 8 (4): 442-450
Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.
View details for DOI 10.1364/OPTICA.416841
View details for PubMedID 34239948
View details for PubMedCentralID PMC8243577
- 3D super-resolution deep-tissue imaging in living mice OPTICA 2021; 8 (4): 442-450