Andrew Barentine

All Publications

• 3D super-resolution deep-tissue imaging in living mice. Optica Velasco, M. G., Zhang, M., Antonello, J., Yuan, P., Allgeyer, E. S., May, D., M'Saad, O., Kidd, P., Barentine, A. E., Greco, V., Grutzendler, J., Booth, M. J., Bewersdorf, J. 2021; 8 (4): 442-450

  Abstract

  Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.

  View details for DOI 10.1364/OPTICA.416841

  View details for PubMedID 34239948

  View details for PubMedCentralID PMC8243577

• 3D super-resolution deep-tissue imaging in living mice OPTICA Velasco, M. M., Zhang, M., Antonello, J., Yuan, P., Allgeyer, E. S., May, D., M'Saad, O., Kidd, P., Barentine, A. S., Greco, V., Grutzendler, J., Booth, M. J., Bewersdorf, J. 2021; 8 (4): 442-450

  View details for DOI 10.1364/OPTICA.416841

  View details for Web of Science ID 000642200300003