Boards, Advisory Committees, Professional Organizations


  • Reviewer, ChemistrySelect (2020 - Present)

Professional Education


  • Master of Science, Indian Institute of Science Education and Research (2015)
  • Doctor of Philosophy, Cornell University (2021)
  • Bachelor of Science, Indian Institute of Science Education and Research (2015)
  • Master of Science, Cornell University (2017)
  • Doctor of Philosophy, Cornell University, Chemistry and Chemical Biology (2021)
  • Dual BS-MS, Indian Institute of Science Education and Research (IISER) - Kolkata, Chemistry (2015)

Stanford Advisors


Patents


  • Bibudha Parasar, Lin Han, Pamela V Chang. "United States Patent 17/231, 432 Compositions and methods for profiling of gut-microbiota associated bile salt hydrolase (BSH) activity"

All Publications


  • Engineered Th17 Cell Differentiation Using a Photoactivatable Immune Modulator JOURNAL OF THE AMERICAN CHEMICAL SOCIETY Parasar, B., Chang, P. 2020; 142 (42): 18103-18108

    Abstract

    T helper 17 (Th17) cells, an important subset of CD4+ T cells, help to eliminate extracellular infectious pathogens that have invaded our tissues. Despite the critical roles of Th17 cells in immunity, how the immune system regulates the production and maintenance of this cell type remains poorly understood. In particular, the plasticity of these cells or their dynamic ability to trans-differentiate into other CD4+ T cell subsets remains mostly uncharacterized. Here, we report a synthetic immunology approach using a photoactivatable immune modulator (PIM) to increase Th17 cell differentiation on demand with spatial and temporal precision to help elucidate this important and dynamic process. In this chemical strategy, we developed a latent agonist that upon photochemical activation releases a small-molecule ligand that targets the aryl hydrocarbon receptor (AhR) and ultimately induces Th17 cell differentiation. We used this chemical tool to control AhR activation with spatiotemporal precision within cells and to modulate Th17 cell differentiation on demand using UV light illumination. We envision that this approach will enable an understanding of the dynamic functions and behaviors of Th17 cells in vivo during immune responses and in mouse models of inflammatory disease.

    View details for DOI 10.1021/jacs.0c07485

    View details for Web of Science ID 000580559000034

    View details for PubMedID 32975936

  • Integrated Regulation of HuR by Translation Repression and Protein Degradation Determines Pulsatile Expression of p53 Under DNA Damage ISCIENCE Guha, A., Ahuja, D., Das Mandal, S., Parasar, B., Deyasi, K., Roy, D., Sharma, V., Willard, B., Ghosh, A., Ray, P. 2019; 15: 342-+

    Abstract

    Expression of tumor suppressor p53 is regulated at multiple levels, disruption of which often leads to cancer. We have adopted an approach combining computational systems modeling with experimental validation to elucidate the translation regulatory network that controls p53 expression post DNA damage. The RNA-binding protein HuR activates p53 mRNA translation in response to UVC-induced DNA damage in breast carcinoma cells. p53 and HuR levels show pulsatile change post UV irradiation. The computed model fitted with the observed pulse of p53 and HuR only when hypothetical regulators of synthesis and degradation of HuR were incorporated. miR-125b, a UV-responsive microRNA, was found to represses the translation of HuR mRNA. Furthermore, UV irradiation triggered proteasomal degradation of HuR mediated by an E3-ubiquitin ligase tripartite motif-containing 21 (TRIM21). The integrated action of miR-125b and TRIM21 constitutes an intricate control system that regulates pulsatile expression of HuR and p53 and determines cell viability in response to DNA damage.

    View details for DOI 10.1016/j.isci.2019.05.002

    View details for Web of Science ID 000470104600030

    View details for PubMedID 31103853

    View details for PubMedCentralID PMC6548907

  • Chemoproteomic Profiling of Gut Microbiota-Associated Bile Salt Hydrolase Activity ACS CENTRAL SCIENCE Parasar, B., Zhou, H., Xiao, X., Shi, Q., Brito, I. L., Chang, P. 2019; 5 (5): 867-873

    Abstract

    The metagenome of the gut microbiome encodes tremendous potential for biosynthesizing and transforming small-molecule metabolites through the activities of enzymes expressed by intestinal bacteria. Accordingly, elucidating this metabolic network is critical for understanding how the gut microbiota contributes to health and disease. Bile acids, which are first biosynthesized in the liver, are modified in the gut by enzymes expressed by commensal bacteria into secondary bile acids, which regulate myriad host processes, including lipid metabolism, glucose metabolism, and immune homeostasis. The gateway reaction of secondary bile acid biosynthesis is mediated by bile salt hydrolases (BSHs), bacterial cysteine hydrolases whose action precedes other bile acid modifications within the gut. To assess how changes in bile acid metabolism mediated by certain intestinal microbiota impact gut physiology and pathobiology, methods are needed to directly examine the activities of BSHs because they are master regulators of intestinal bile acid metabolism. Here, we developed chemoproteomic tools to profile changes in gut microbiome-associated BSH activity. We showed that these probes can label active BSHs in model microorganisms, including relevant gut anaerobes, and in mouse gut microbiomes. Using these tools, we identified altered BSH activities in a murine model of inflammatory bowel disease, in this case, colitis induced by dextran sodium sulfate, leading to changes in bile acid metabolism that could impact host metabolism and immunity. Importantly, our findings reveal that alterations in BSH enzymatic activities within the gut microbiome do not correlate with changes in gene abundance as determined by metagenomic sequencing, highlighting the utility of chemoproteomic approaches for interrogating the metabolic activities of the gut microbiota.

    View details for DOI 10.1021/acscentsci.9b00147

    View details for Web of Science ID 000468624100018

    View details for PubMedID 31139722

    View details for PubMedCentralID PMC6535767

  • Chemical optogenetic modulation of inflammation and immunity CHEMICAL SCIENCE Parasar, B., Chang, P. V. 2017; 8 (2): 1450-1453

    Abstract

    The immune system is an essential component of host defense against pathogens and is largely mediated by inflammatory molecules produced by immune cells, such as macrophages. These inflammatory mediators are regulated at the transcriptional level by chromatin-modifying enzymes including histone deacetylases (HDACs). Here we describe a strategy to regulate inflammation and immunity with photocontrolled HDAC inhibitors, which can be selectively delivered to target cells by UV irradiation to minimize off-target effects. We strategically photocaged the active moiety of an HDAC inhibitor and showed that mild UV irradiation leads to the selective release of the inhibitor in a spatiotemporal manner. This methodology was used to decrease the amount of pro-inflammatory mediators produced by a subpopulation of macrophages. Our approach could ultimately be used to control inflammation in vivo as a therapeutic for inflammatory diseases, while minimizing off-target effects to healthy tissues.

    View details for DOI 10.1039/c6sc03702j

    View details for Web of Science ID 000395428300073

    View details for PubMedID 28451285

    View details for PubMedCentralID PMC5390787

  • Finding the Sweet Spot for Breast Cancer Detection ACS CENTRAL SCIENCE Parasar, B., Chang, P. V. 2020; 6 (12): 2123-2125

    View details for DOI 10.1021/acscentsci.0c01439

    View details for Web of Science ID 000603399200003

    View details for PubMedID 33376773

    View details for PubMedCentralID PMC7760063

  • Microwave assisted cross-coupling reactions using palladium nanoparticles in aqueous media SYNTHETIC COMMUNICATIONS Dhara, K., Parasar, B., Patil, A. J., Dash, J. 2019; 49 (6): 859-868
  • Bipyridine Facilitates Regioselective Dimerization of Alkynes in the Absence of Transition Metal CHEMISTRYSELECT Midya, G., Parasar, B., Maiti, S., Dash, J. 2017; 2 (18): 5032-5037
  • Transcript degradation and noise of small RNA-controlled genes in a switch activated network in Escherichia coli NUCLEIC ACIDS RESEARCH Arbel-Goren, R., Tal, A., Parasar, B., Dym, A., Costantino, N., Munoz-Garcia, J., Court, D. L., Stavans, J. 2016; 44 (14): 6707-6720

    Abstract

    Post-transcriptional regulatory processes may change transcript levels and affect cell-to-cell variability or noise. We study small-RNA downregulation to elucidate its effects on noise in the iron homeostasis network of Escherichia coli In this network, the small-RNA RyhB undergoes stoichiometric degradation with the transcripts of target genes in response to iron stress. Using single-molecule fluorescence in situ hybridization, we measured transcript numbers of the RyhB-regulated genes sodB and fumA in individual cells as a function of iron deprivation. We observed a monotonic increase of noise with iron stress but no evidence of theoretically predicted, enhanced stoichiometric fluctuations in transcript numbers, nor of bistable behavior in transcript distributions. Direct detection of RyhB in individual cells shows that its noise is much smaller than that of these two targets, when RyhB production is significant. A generalized two-state model of bursty transcription that neglects RyhB fluctuations describes quantitatively the dependence of noise and transcript distributions on iron deprivation, enabling extraction of in vivo RyhB-mediated transcript degradation rates. The transcripts' threshold-linear behavior indicates that the effective in vivo interaction strength between RyhB and its two target transcripts is comparable. Strikingly, the bacterial cell response exhibits Fur-dependent, switch-like activation instead of a graded response to iron deprivation.

    View details for DOI 10.1093/nar/gkw273

    View details for Web of Science ID 000382999900020

    View details for PubMedID 27085802

    View details for PubMedCentralID PMC5001584

  • Chiral carbon dots derived from guanosine 5 '-monophosphate form supramolecular hydrogels CHEMICAL COMMUNICATIONS Ghosh, A., Parasar, B., Bhattacharyya, T., Dash, J. 2016; 52 (74): 11159-11162

    Abstract

    Guanosine 5'-monophosphate, (5'-GMP), is a self-assembling natural nucleotide that has unique potential to form ordered supramolecular structures. We herein describe an intriguing property of Na2(5'-GMP) to form blue emitting chiral carbon dots (G-dots) that exhibit excitation dependent down-conversion and up-conversion fluorescence signature and self-assemble to form fluorescent hydrogels.

    View details for DOI 10.1039/c6cc05947c

    View details for Web of Science ID 000384202000029

    View details for PubMedID 27560935

  • A copper based catalyst for poly-urethane synthesis from discarded motherboard RSC ADVANCES Barman, S., Parasar, B., Kundu, P., Roy, S. 2016; 6 (79): 75749-75756

    View details for DOI 10.1039/c6ra14506j

    View details for Web of Science ID 000381513800091

  • MICRO-OPTOMECHANICAL MOVEMENTS (MOMs) WITH SOFT OXOMETALATES (SOMs): CONTROLLED MOTION OF SINGLE SOFT OXOMETALATE PEAPODS USING EXOTIC OPTICAL POTENTIALS JOURNAL OF MOLECULAR AND ENGINEERING MATERIALS Roy, B., Ghosh, N., Panigrahi, P. K., Banerjee, A., Sahasrabudhe, A., Parasar, B., Roy, S. 2014; 2 (1)
  • Ligand mediated iron catalyzed dimerization of terminal aryl alkynes: scope and limitations ORGANIC & BIOMOLECULAR CHEMISTRY Midya, G., Parasar, B., Dhara, K., Dash, J. 2014; 12 (11): 1812-1822

    Abstract

    Regioselective dimerization of terminal aryl alkynes to produce conjugated enynes has been achieved using FeCl3 and KO(t)Bu in the presence of either DMEDA or dppe. The reaction proceeds smoothly in toluene at 145 °C for 2 h to give the corresponding head-to-head dimers in good to excellent yields (54 to 99%) with high E-selectivity (67 : 33 to 83 : 17 E/Z). Both strongly electron-donating and electron-withdrawing groups are compatible with this procedure. The bidentate phosphine (dppe) ligand exhibits better catalytic activity than the bidentate amine (DMEDA). The aliphatic acetylene fails to react under this catalytic system which suggests that potassium tertiary butoxide activates the conjugated system of aryl acetylene through cation-pi interaction and pi-pi interaction. A radical inhibitor (galvinoxyl or TEMPO) completely suppresses the reaction. Employing FeCl2 as a catalyst instead of FeCl3, only phenyl acetylene afforded the corresponding head to head dimer in good yield. Mechanistic pathways for both FeCl3 catalyzed dimerization of aryl alkynes and FeCl2 catalyzed dimerization of phenyl acetylene have been proposed.

    View details for DOI 10.1039/c3ob42365d

    View details for Web of Science ID 000332225500018

    View details for PubMedID 24514585

  • pH dependent multifunctional and multiply-configurable logic gate systems based on small molecule G-quadruplex DNA recognition CHEMICAL COMMUNICATIONS Bhowmik, S., Das, R., Parasar, B., Dash, J. 2013; 49 (18): 1817-1819

    Abstract

    A variety of logic operations such as XNOR, NOR, AND, NAND, NOT have been designed with pH as an external modulator by choosing thiazole orange (TO) and a c-kit2 promoter quadruplex as two inputs and fluorescence signals of pyridyl bis-indole amide (PBIA) and TO as two outputs.

    View details for DOI 10.1039/c3cc38888c

    View details for Web of Science ID 000314650100010

    View details for PubMedID 23358682

  • Reduction of organic azides to amines using reusable Fe3O4 nanoparticles in aqueous medium CATALYSIS SCIENCE & TECHNOLOGY Pagoti, S., Surana, S., Chauhan, A., Parasar, B., Dash, J. 2013; 3 (3): 584-588

    View details for DOI 10.1039/c2cy20776a

    View details for Web of Science ID 000314782000007