Brendan Wirtz
Ph.D. Student in Chemical Engineering, admitted Autumn 2021
Education & Certifications
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MS, Stanford University, Chemical Engineering (2024)
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BS, University of California, Berkeley, Chemical Engineering (2021)
All Publications
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Protease-Driven Phase Separation of Elastin-Like Polypeptides.
Biomacromolecules
2024
Abstract
Elastin-like polypeptides (ELPs) are a promising material platform for engineering stimuli-responsive biomaterials, as ELPs undergo phase separation above a tunable transition temperature. ELPs with phase behavior that is isothermally regulated by biological stimuli remain attractive for applications in biological systems. Herein, we report protease-driven phase separation of ELPs. Protease-responsive "cleavable" ELPs comprise a hydrophobic ELP block connected to a hydrophilic ELP block by a protease cleavage site linker. The hydrophilic ELP block acts as a solubility tag for the hydrophobic ELP block, creating a temperature window in which the cleavable ELP reactant is soluble and the proteolytically generated hydrophobic ELP block is insoluble. Within this temperature window, isothermal, protease-driven phase separation occurs when a critical concentration of hydrophobic cleavage product accumulates. Furthermore, protease-driven phase separation is generalizable to four compatible protease-cleavable ELP pairings. This work presents exciting opportunities to regulate ELP phase behavior in biological systems using proteases.
View details for DOI 10.1021/acs.biomac.4c00346
View details for PubMedID 38980747
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Spatially Controlled Uv Light Generation at Depth Using Upconversion Micelles.
Advanced materials (Deerfield Beach, Fla.)
2023: e2301563
Abstract
Ultraviolet (UV) light can trigger a plethora of useful photochemical reactions for diverse applications, including photocatalysis, photopolymerization, and drug delivery. These applications typically require penetration of high energy photons deep into materials, yet delivering these photons beyond the surface is extremely challenging due to absorption and scattering effects. Triplet-triplet annihilation upconversion (TTA-UC) shows great promise to circumvent this issue by generating high energy photons from incident lower energy photons. However, molecules that facilitate TTA-UC usually have poor water solubility, limiting their deployment in aqueous environments. To address this challenge, a nanoencapsulation method is leveraged to fabricate water-compatible UC micelles, enabling on-demand UV photon generation deep into materials. Two iridium-based complexes are presented for use as TTA-UC sensitizers with increased solubilities that facilitate the formation of highly emissive UV-upconverting micelles. Furthermore, this encapsulation method is shown to be generalizable to nineteen UV-emitting UC systems, accessing a range of upconverted UV emission profiles with wavelengths as low as 350 nm. As a proof-of-principle demonstration of precision photochemistry at depth, UV-emitting UC micelles are used to photolyze a fluorophore at a focal point nearly a centimeter beyond the surface, revealing opportunities for spatially controlled manipulation deep into UV-responsive materials. This article is protected by copyright. All rights reserved.
View details for DOI 10.1002/adma.202301563
View details for PubMedID 37548335
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High-throughput Li plating quantification for fast-charging battery design
NATURE ENERGY
2023
View details for DOI 10.1038/s41560-023-01194-y
View details for Web of Science ID 000924735200001