School of Medicine

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  • John Gorzynski

    John Gorzynski

    Postdoctoral Scholar, Genetics
    Life Science Research Professional, Medicine - Med/Cardiovascular Medicine

    BioVeterinarian and Researcher with a keen interest in genetic determinants of cardiomyopathies in great apes

  • Tuhin Guha

    Tuhin Guha

    Postdoctoral Scholar, Genetics

    BioA research individual with more than ten years of extensive research experience in molecular biology techniques ranging from DNA/RNA manipulations, recombinant protein expression, purification, and biochemical characterization of DNA-cutting enzymes, needed for genetic engineering. I am a postdoctoral research fellow in the Department of Genetics, Stanford University. I work with Dr. Mike Snyder. My research involves characterization of tissue and tumor microenvironment at single-cell resolution using CODEX multiplexed-molecular imaging on precancerous and cancerous colon polyps (Familial adenomatous polyposis) along with studying aging heart, placenta microenvironment. My previous work involved novel gene therapy approaches to cure muscle disorders, particularly Limb-girdle muscular dystrophy 2A using mouse models, and gene repair studies using modified CRISPR system through plasmid/protein-oligo based transfections, electroporation in human cells, microinjection in frog embryos and analyzing editing efficiencies using flow cytometry. I have a multi-disciplinary background, therefore I have a solid understanding and working knowledge in a broader domain within the biological sciences, be it from animal behavior, ecology, gene regulation, genetic diseases to understanding and designing “molecular switches” within DNA-cutting proteins, such as meganucleases, and CRISPR/Cas9 for genome engineering. I have strong communication skills, presented my research in various conferences and published heavily in the field of DNA-cutting enzymes and their use as genome editing tools. I have published in 15 peer-reviewed journals and also reviewed 9 research papers. I have a proven ability to manage challenging research objectives, collaborated with other research teams, and delivered results effectively. I always welcome new ideas and interact with people to learn any new skills and experiences. I have also supervised several undergraduate project students, summer students and junior graduate students.