All Publications

  • Simultaneous Multiomic Single-Cell Analysis Reveals Gene Regulatory Mechanism of Interleukin-Based Immunotherapy in Pancreatic Ductal Adenocarcinoma Guo, C., Morgan, A., Griffin, M., Parker, J. L., Januszyk, M., Foster, D., Delitto, D., Longaker, M. T., Norton, J. A. LIPPINCOTT WILLIAMS & WILKINS. 2024: S456

    View details for Web of Science ID 001348680703113

  • Organoid Assessment of IL-1 Inhibition in Human Pancreatic Ductal Adenocarcinoma Morgan, A., Griffin, M., Guo, C., Parker, J. L., Januszyk, M., Foster, D. S., Poultsides, G. A., Delitto, D., Longaker, M. T., Norton, J. A. LIPPINCOTT WILLIAMS & WILKINS. 2024: S451-S452

    View details for Web of Science ID 001348680703104

  • Self-Reporting Transposons Enable Simultaneous Readout of Gene Expression and Transcription Factor Binding in Single Cells CELL Moudgil, A., Wilkinson, M. N., Chen, X., He, J., Cammack, A. J., Vasek, M. J., Lagunas, T., Qi, Z., Lalli, M. A., Guo, C., Morris, S. A., Dougherty, J. D., Mitra, R. D. 2020; 182 (4): 992-+

    Abstract

    Cellular heterogeneity confounds in situ assays of transcription factor (TF) binding. Single-cell RNA sequencing (scRNA-seq) deconvolves cell types from gene expression, but no technology links cell identity to TF binding sites (TFBS) in those cell types. We present self-reporting transposons (SRTs) and use them in single-cell calling cards (scCC), a novel assay for simultaneously measuring gene expression and mapping TFBS in single cells. The genomic locations of SRTs are recovered from mRNA, and SRTs deposited by exogenous, TF-transposase fusions can be used to map TFBS. We then present scCC, which map SRTs from scRNA-seq libraries, simultaneously identifying cell types and TFBS in those same cells. We benchmark multiple TFs with this technique. Next, we use scCC to discover BRD4-mediated cell-state transitions in K562 cells. Finally, we map BRD4 binding sites in the mouse cortex at single-cell resolution, establishing a new method for studying TF biology in situ.

    View details for DOI 10.1016/j.cell.2020.06.037

    View details for Web of Science ID 000561488500018

    View details for PubMedID 32710817

    View details for PubMedCentralID PMC7510185

  • CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics. Genome biology Guo, C., Kong, W., Kamimoto, K., Rivera-Gonzalez, G. C., Yang, X., Kirita, Y., Morris, S. A. 2019; 20 (1): 90

    Abstract

    High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are heritable, enabling cell populations to be tagged, pooled, and tracked over time in the same experimental replicate. We demonstrate the utility of CellTag Indexing by sequencing transcriptomes using a variety of cell types, including long-term tracking of cell engraftment and differentiation in vivo. Together, this presents CellTag Indexing as a broadly applicable genetic multiplexing tool that is complementary with existing single-cell technologies.

    View details for DOI 10.1186/s13059-019-1699-y

    View details for PubMedID 31072405

    View details for PubMedCentralID PMC6509836

  • Single-cell mapping of lineage and identity in direct reprogramming. Nature Biddy, B. A., Kong, W., Kamimoto, K., Guo, C., Waye, S. E., Sun, T., Morris, S. A. 2018; 564 (7735): 219-224

    Abstract

    Direct lineage reprogramming involves the conversion of cellular identity. Single-cell technologies are useful for deconstructing the considerable heterogeneity that emerges during lineage conversion. However, lineage relationships are typically lost during cell processing, complicating trajectory reconstruction. Here we present 'CellTagging', a combinatorial cell-indexing methodology that enables parallel capture of clonal history and cell identity, in which sequential rounds of cell labelling enable the construction of multi-level lineage trees. CellTagging and longitudinal tracking of fibroblast to induced endoderm progenitor reprogramming reveals two distinct trajectories: one leading to successfully reprogrammed cells, and one leading to a 'dead-end' state, paths determined in the earliest stages of lineage conversion. We find that expression of a putative methyltransferase, Mettl7a1, is associated with the successful reprogramming trajectory; adding Mettl7a1 to the reprogramming cocktail increases the yield of induced endoderm progenitors. Together, these results demonstrate the utility of our lineage-tracing method for revealing the dynamics of direct reprogramming.

    View details for DOI 10.1038/s41586-018-0744-4

    View details for PubMedID 30518857

    View details for PubMedCentralID PMC6635140

  • Engineering cell identity: establishing new gene regulatory and chromatin landscapes. Current opinion in genetics & development Guo, C., Morris, S. A. 2017; 46: 50-57

    Abstract

    Cellular reprogramming can be achieved by ectopically expressing transcription factors that directly convert one differentiated cell type into another, bypassing embryonic states. A number of different cell types have been generated by such 'direct lineage reprogramming' methods, but their practical utility has been limited because, in most protocols, the resulting populations are often partially differentiated or incompletely specified. Here, we review mechanisms of lineage reprogramming by pioneer transcription factors, a unique class of transcriptional regulators that has the capacity to engage with silent chromatin to activate target gene regulatory networks. We assess the possible barriers to successful reprogramming in the context of higher-order chromatin landscape, considering how the mechanistic relationship between nuclear organization and cell identity will be crucial to unlocking the full potential of cell fate engineering.

    View details for DOI 10.1016/j.gde.2017.06.011

    View details for PubMedID 28667865

  • Cardiomyocyte-specific role of miR-24 in promoting cell survival. Journal of cellular and molecular medicine Guo, C., Deng, Y., Liu, J., Qian, L. 2015; 19 (1): 103-12

    Abstract

    Cardiomyocyte cell death is a major contributing factor to various cardiovascular diseases and is therefore an important target for the design of therapeutic strategies. More recently, stem cell therapies, such as transplantation of embryonic or induced pluripotent stem (iPS) cell-derived cardiomyocytes, have emerged as a promising alternative therapeutic avenue to treating cardiovascular diseases. Nevertheless, survival of these introduced cells is a serious issue that must be solved before clinical application. We and others have identified a small non-coding RNA, microRNA-24 (miR-24), as a pro-survival molecule that inhibits the apoptosis of cardiomyocytes. However, these earlier studies delivered mimics or inhibitors of miR-24 via viral transduction or chemical transfection, where the observed protective role of miR-24 in cardiomyocytes might have partially resulted from its effect on non-cardiomyocyte cells. To elucidate the cardiomyocyte-specific effects of miR-24 when overexpressed, we developed a genetic model by generating a transgenic mouse line, where miR-24 expression is driven by the cardiac-specific Myh6 promoter. The Myh6-miR-24 transgenic mice did not exhibit apparent difference from their wild-type littermates under normal physiological conditions. However, when the mice were subject to myocardial infarction (MI), the transgenic mice exhibited decreased cardiomyocyte apoptosis, improved cardiac function and reduced scar size post-MI compared to their wild-type littermates. Interestingly, the protective effects observed in our transgenic mice were smaller than those from earlier reported approaches as well as our parallelly performed non-genetic approach, raising the possibility that non-genetic approaches of introducing miR-24 might have been mediated via other cell types than cardiomyocytes, leading to a more dramatic phenotype. In conclusion, our study for the first time directly tests the cardiomyocyte-specific role of miR-24 in the adult heart, and may provide insight to strategy design when considering miRNA-based therapies for cardiovascular diseases.

    View details for DOI 10.1111/jcmm.12393

    View details for PubMedID 25352422

    View details for PubMedCentralID PMC4288354

  • Direct Somatic Cell Reprogramming: Treatment of Cardiac Diseases CURRENT GENE THERAPY Guo, C., Patel, K., Qian, L. 2013; 13 (2): 133-138

    Abstract

    Cardiac diseases are the major causes of morbidity and mortality in the world. Cardiomyocyte death is a common consequence of many types of heart diseases and is usually irreversible. Scar tissues formed by cardiac fibroblasts serve compensatory roles for the injured heart but eventually weaken cardiac function and result in life-threatening heart failures. Unfortunately, adult human hearts have limited regenerative capacities. In the past decades, many interventional approaches have been taken in an attempt to restore functional cardiomyocytes in an injured heart. Promising advances have been made in directly reprogramming mouse fibroblasts into cardiomyocyte-like cells both in vitro and in vivo. Recently, several different methods have been reported, including the use of transcription factors and microRNAs. In addition, two in vivo studies showed heart function improvements with delivery of reprogramming factors in mouse infarcted hearts. Although many of these studies are at early preliminary stages, the plausibility of applying cardiac reprogramming on patients for regenerative purposes is exciting, and may lead to numerous novel research directions in the field. This review will discuss the history, recent advances and challenges of cellular reprogramming, specifically in the field of cardiac regeneration.

    View details for DOI 10.2174/1566523211313020007

    View details for Web of Science ID 000317846100007

    View details for PubMedID 23320478