Visiting Instructor/Lecturer, Medicine - Immunology & Rheumatology
- Clinical Efficacy Observation of Humanized BCMA-CAR-T in the Treatment of Relapsed/Refractory Multiple Myeloma Complicated with Extramedullary Lesions AMER SOC HEMATOLOGY. 2022: 10287
- CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming CELL STEM CELL 2018; 23 (5): 758-+
CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming.
Cell stem cell
Comprehensive identification of factors that can specify neuronal fate could provide valuable insights into lineage specification and reprogramming, but systematic interrogation of transcription factors, and their interactions with each other, has proven technically challenging. We developed a CRISPR activation (CRISPRa) approach to systematically identify regulators of neuronal-fate specification. We activated expression of all endogenous transcription factors and other regulators via a pooled CRISPRa screen in embryonic stem cells, revealing genes including epigenetic regulators such as Ezh2 that can induce neuronal fate. Systematic CRISPR-based activation of factor pairs allowed us to generate a genetic interaction map for neuronal differentiation, with confirmation of top individual and combinatorial hits as bona fide inducers of neuronal fate. Several factor pairs could directly reprogram fibroblasts into neurons, which shared similar transcriptional programs with endogenous neurons. This study provides an unbiased discovery approach for systematic identification of genes that drive cell-fate acquisition.
View details for PubMedID 30318302
[Application of genome engineering in medical synthetic biology].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
2017; 33 (3): 422-435
Synthetic biology aims to establish a complete set of engineering principles, theories, and methods, via the rational design and assembly of basic biological parts, for the goal of effective implementation of complex biological systems with programmable functions. In recent years, with emerging novel classes of programmable genetic parts, in particular, the establishment and optimization of CRISPR and CRISPRi technology platforms, synthetic biology is entering a new era. This review summarizes recent advances on CRISPR genome editing and gene regulation technologies, their applications in constructing programmable biological parts, and their roles in building sophisticated gene circuits. We also provide a future vision on how synthetic biology can transform medicine (named medical synthetic biology, MSB) and therapeutics.
View details for DOI 10.13345/j.cjb.160420
View details for PubMedID 28941341
Applications of CRISPR Genome Engineering in Cell Biology.
Trends in cell biology
2016; 26 (11): 875-888
Recent advances in genome engineering are starting a revolution in biological research and translational applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated RNA-guided endonuclease CRISPR associated protein 9 (Cas9) and its variants enable diverse manipulations of genome function. In this review, we describe the development of Cas9 tools for a variety of applications in cell biology research, including the study of functional genomics, the creation of transgenic animal models, and genomic imaging. Novel genome engineering methods offer a new avenue to understand the causality between the genome and phenotype, thus promising a fuller understanding of cell biology.
View details for DOI 10.1016/j.tcb.2016.08.004
View details for PubMedID 27599850
View details for PubMedCentralID PMC5077632