Detlef Obal, Postdoctoral Faculty Sponsor
Generation of two induced pluripotent stem cell lines from patients suffering from pulmonary hypertension.
Stem cell research
2023; 72: 103218
Idiopathic pulmonary arterial hypertension (IPAH) is a rare disease, with an estimated 500-1000 new cases diagnosed every year. A portion of these cases may be caused by mutations in the BMPR2 gene, suggesting a possible genetic component in the development of the disease. Here, we report two human induced pluripotent stem cell (iPSC) lines generated from IPAH patients. Both cell lines provide valuable insight into the molecular and cellular mechanisms of IPAH and can be used to further understand the disease.
View details for DOI 10.1016/j.scr.2023.103218
View details for PubMedID 37804546
Detecting and measuring of GPCR signaling - comparison of human induced pluripotent stem cells and immortal cell lines.
Frontiers in endocrinology
2023; 14: 1179600
G protein-coupled receptors (GPCRs) are a large family of transmembrane proteins that play a major role in many physiological processes, and thus GPCR-targeted drug development has been widely promoted. Although research findings generated in immortal cell lines have contributed to the advancement of the GPCR field, the homogenous genetic backgrounds, and the overexpression of GPCRs in these cell lines make it difficult to correlate the results with clinical patients. Human induced pluripotent stem cells (hiPSCs) have the potential to overcome these limitations, because they contain patient specific genetic information and can differentiate into numerous cell types. To detect GPCRs in hiPSCs, highly selective labeling and sensitive imaging techniques are required. This review summarizes existing resonance energy transfer and protein complementation assay technologies, as well as existing and new labeling methods. The difficulties of extending existing detection methods to hiPSCs are discussed, as well as the potential of hiPSCs to expand GPCR research towards personalized medicine.
View details for DOI 10.3389/fendo.2023.1179600
View details for PubMedID 37293485
View details for PubMedCentralID PMC10244570
- DNA-modularized construction of bivalent ligands precisely regulates receptor binding and activation CHEM 2023; 9 (4): 901-923