Gunilla B Jacobson
Director, Translational Medicine and Technical & Strategic Director, Cyclotron, Rad/Precision Health and Integrated Diagnostics
Current Role at Stanford
Director of Translational Medicine, DeSimone Lab
Technical and Strategic Director, Cyclotron
Education & Certifications
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Postdoctoral Fellow, Los Alamos National Laboratory, Chemistry (1998)
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Postdoctoral Fellow, University of Texas, Chemical Engineering (1997)
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PhD, Uppsala University, Sweden, Organic Chemistry (1996)
Patents
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Gunilla Jacobson, W. Tumas, K. Johnston. "United States Patent 6,479,708 Biphasic Catalysis in Water/Carbon Dioxide Micellar Systems.", Los Alamos National Laboratory
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Glenn Gale, Joe T. Hillman, Gunilla Jacobson, Bentley Palmer,. "United States Patent 7,250,374 System and Method for Processing a Substrate Using Supercritical Carbon Dioxide Processing.", TEL Ltd.
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Gunilla Jacobson, Deborah Yellowaga. "United States Patent 7,387,868 Treatment of a Dielectric Layer Using Supercritical Carbon Dioxide.", TEL Ltd
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Tatsiana Lobovkina, Richard N, Gunilla B Jacobson, Ramin E. Beygui, Evgenios Neofytou.. "United States Patent US 20130243848 A1 Nanoparticles, Nanoparticles Delivery Methods, and Systems of Delivery.", Stanford University, Mar 13, 2013
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Gunilla Jacobson, R.N. Zare, K. E. Markides, C. Contag. "United States Patent US 2008/0095856 A1 Encapsulated Nanoparticles for Drug Delivery.", Stanford University, May 14, 2007
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Gunilla Jacobson, Subramanyam A Iyer. "United States Patent US 2007/0000519 A1 Removal of Residues from Low-k Dielectric Material in Wafer Processing.", Supercritical Systems Inc., Jun 30, 2005
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Gunilla Jacobson, Robert Kevwitch, Deborah Yellowaga. "United States Patent US 2006/0255012 A1 Removal of Particles from Substrate Surface Using Supercritical Processing", Supercritical Systems Inc., May 10, 2005
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Gunilla Jacobson. "United States Patent US 2006/0219268 Neutralization of Systemic Poisoning in Wafer Processing", Supercritical Systems Inc., Mar 30, 2005
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Gunilla Jacobson, Robert Kevwitch, Marie Lowe. "United States Patent US 2006/0186088 A1 Etching and Cleaning BPSG Material Using Supercritical Processing", Supercritical Systems Inc., Feb 23, 2005
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Gunilla Jacobson, Robert Kevwitch, Deborah Yellowaga. "United States Patent US 2006/0180572 A1 Removal of Post Etch Residue for a Substrate With Open Metal Surfaces", Supercritical Systems Inc., Feb 15, 2005
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Gunilla Jacobson, Bentley Palmer, Shan C. Clark, Vijayakumar S. Ramachandrarao, Subramanyam A. Iyer, Robert Turkot,. "United States Patent US 2006/0102204 A1 Method For Removing A Residue From A Substrate Using Supercritical Carbon Dioxide Processing,", Supercritical Systems Inc., Nov 12, 2004
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Gunilla Jacobson, Bentley Palmer. "United States Patent US 2006/0102208 A1 System For Removing A Residue From A Substrate Using Supercritical Carbon Dioxide Processing", Supercritical Systems Inc., Nov 12, 2004
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Gunilla Jacobson, Marie Lowe, Robert Kevwitch, Brandon Hansen. "United States Patent US 2006/0102591 A1 Method and System For Treating A Substrate Using A Supercritical Fluid", Supercritical Systems Inc., Nov 12, 2004
Work Experience
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Visting Associate Professor, Department of Pediatrics, Stanford School of Medicine (2014 - 2016)
Location
Stanford CA
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Docent, Faculty of Science and Technology, Uppsala University (2014 - Present)
Location
Uppsala, Sweden
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Senior Scientist, Department of Neuroscience, Karolinska Institutet (2011 - 2013)
Location
Stockholm, Sweden
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Senior Research Associate, Department of Chemistry, Stanford University (2004 - 2011)
Location
Stanford, CA
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Process Development Manager, Supercritical Systems / Tokyo Electron, Ltd, (2002 - 2004)
Location
Gilbert AZ
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Technical Staff Member, Los Alamos National Laboratory (2000 - 2002)
Location
Los Alamos NM
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Research Engineer, Uppsala University PET Center
Location
Uppsala, Sweden
All Publications
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3D-Printed Latticed Microneedle Array Patches for Tunable and Versatile Intradermal Delivery.
Advanced materials (Deerfield Beach, Fla.)
2024: e2404606
Abstract
Using high-resolution 3D printing, a novel class of microneedle array patches (MAPs) is introduced, called latticed MAPs (L-MAPs). Unlike most MAPs which are composed of either solid structures or hollow needles, L-MAPs incorporate tapered struts that form hollow cells capable of trapping liquid droplets. The lattice structures can also be coated with traditional viscous coating formulations, enabling both liquid- and solid-state cargo delivery, on a single patch. Here, a library of 43 L-MAP designs is generated and in-silico modeling is used to down-select optimal geometries for further characterization. Compared to traditionally molded and solid-coated MAPs, L-MAPs can load more cargo with fewer needles per patch, enhancing cargo loading and drug delivery capabilities. Further, L-MAP cargo release kinetics into the skin can be tuned based on formulation and needle geometry. In this work, the utility of L-MAPs as a platform is demonstrated for the delivery of small molecules, mRNA lipid nanoparticles, and solid-state ovalbumin protein. In addition, the production of programmable L-MAPs is demonstrated with tunable cargo release profiles, enabled by combining needle geometries on a single patch.
View details for DOI 10.1002/adma.202404606
View details for PubMedID 39221508
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3D-Printed Microarray Patches for Transdermal Applications.
JACS Au
2022; 2 (11): 2426-2445
Abstract
The intradermal (ID) space has been actively explored as a means for drug delivery and diagnostics that is minimally invasive. Microneedles or microneedle patches or microarray patches (MAPs) are comprised of a series of micrometer-sized projections that can painlessly puncture the skin and access the epidermal/dermal layer. MAPs have failed to reach their full potential because many of these platforms rely on dated lithographic manufacturing processes or molding processes that are not easily scalable and hinder innovative designs of MAP geometries that can be achieved. The DeSimone Laboratory has recently developed a high-resolution continuous liquid interface production (CLIP) 3D printing technology. This 3D printer uses light and oxygen to enable a continuous, noncontact polymerization dead zone at the build surface, allowing for rapid production of MAPs with precise and tunable geometries. Using this tool, we are now able to produce new classes of lattice MAPs (L-MAPs) and dynamic MAPs (D-MAPs) that can deliver both solid state and liquid cargos and are also capable of sampling interstitial fluid. Herein, we will explore how additive manufacturing can revolutionize MAP development and open new doors for minimally invasive drug delivery and diagnostic platforms.
View details for DOI 10.1021/jacsau.2c00432
View details for PubMedID 36465529
View details for PubMedCentralID PMC9709783
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3D-Printed Microarray Patches for Transdermal Applications
JACS AU
2022
View details for DOI 10.1021/jacsau.2c00432
View details for Web of Science ID 000874579200001
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Proceedings: Pathways for Successful Translation of New Imaging Agents and Modalities-Phase III Studies
JOURNAL OF NUCLEAR MEDICINE
2019; 60 (6): 736–44
View details for DOI 10.2967/jnumed.118.219824
View details for Web of Science ID 000470084400016
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Proceedings - Pathways for Successful Translation of New Imaging Agents and Modalities: Phase III Studies.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
2019
Abstract
Significant advances continue in the development of cancer-specific molecular imaging agents and modalities for diagnosing, staging, and treating various cancer types. For these advances to successfully translate to widespread clinical use, both regulatory approval by the U.S. Food and Drug Administration (FDA), and completion of the reimbursement approval process with the Centers for Medicare & Medicaid Services (CMS), must be achieved. To this end, methods for expediting these processes are being addressed by both agencies. This report summarizes a one-day meeting held May 15, 2017 with officials from the National Cancer Institute (NCI), FDA, and CMS; members of the Society of Nuclear Medicine and Molecular Imaging (SNMMI), the World Molecular Imaging Society (WMIS), the American College of Radiology (ACR), the Radiological Society of North America (RSNA), the International Society for Strategic Studies in Radiology (IS3R), the Medical Imaging and Technology Alliance (MITA), molecular imaging scientists from some major hospitals/institutions and industry representatives, who joined together to learn about the pathways of approval, coverage, and payment decisions. The meeting's focus was three-part: i) to broaden the conversation to other agents/modalities and address both the reimbursement and regulatory components; ii) to present a current view of FDA regulatory innovations in cancer imaging development, a past and present view of PET/MI agent approval processes, and a current view of CMS reimbursement protocols; and iii) to discuss scientific issues involved in orchestrating agency-required Phase III clinical trials as presented through three case examples: a PET probe targeting the prostate specific membrane antigen (PSMA) for imaging prostate cancer, an optical antibody probe for image-guided head and neck cancer surgery targeting epidermal growth factor receptor (EGFR), and ultrasonography with targeted microbubbles against a neovasculature target, kinase insert domain receptor (KDR), for ovarian cancer. Additionally, the meeting served to provide more clarity to researchers on trial design, to delineate more uniformly required steps investigators should take to successfully translate their innovations. Reported here are the meeting's discussion items and recommendations for continued honing of the approval and reimbursement processes investigators must navigate.
View details for PubMedID 30850482
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Electrochemically triggered release of acetylcholine from scCO2 impregnated conductive polymer films evokes intracellular Ca(2+) signaling in neurotypic SH-SY5Y cells.
Journal of controlled release
2016; 243: 283-290
Abstract
Implantable devices for electronically triggered drug release are attractive to achieve spatial and temporal control over drug concentrations in patients. Realization of such devices is, however, associated with technical and biological challenges. Among these are containment of drug reservoirs, lack of precise control cues, as well as the charge and size of the drug. Here, we present a method for electronically triggered release of the quaternary ammonium cation acetylcholine (ACh) from an impregnated conductive polymer film. Using supercritical carbon dioxide (scCO2), a film of PEDOT/PSS (poly(3,4)-ethylenedioxythiophene doped with poly(styrenesulfonate)) is impregnated with the neurotransmitter acetylcholine. The gentle scCO2 process generated a dry, drug-impregnated surface, well suited for interaction with biological material, while maintaining normal electrochemical properties of the polymer. Electrochemical switching of impregnated PEDOT/PSS films stimulated release of ACh from the polymer matrix, likely due to swelling mediated by the influx and efflux of charged and solvated ions. Triggered release of ACh did not affect the biological activity of the drug. This was shown by real-time monitoring of intracellular Ca(2+) signaling in neurotypic cells growing on the impregnated polymer surface. Collectively, scCO2 impregnation of conducting polymers offers the first one-step, dopant-independent drug impregnation process, potentially facilitating loading of both anionic and cationic drugs that can be dissolved in scCO2 on its own or by using a co-solvent. We foresee that scCO2-loaded devices for electronically triggered drug release will create novel opportunities when generating active bio-coatings, tunable for specific needs, in a variety of medical settings.
View details for DOI 10.1016/j.jconrel.2016.10.020
View details for PubMedID 27793684
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Scaffold-mediated BMP-2 minicircle DNA delivery accelerated bone repair in a mouse critical-size calvarial defect model
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2016; 104 (8): 2099-2107
Abstract
Scaffold-mediated gene delivery holds great promise for tissue regeneration. However, previous attempts to induce bone regeneration using scaffold-mediated non-viral gene delivery rarely resulted in satisfactory healing. We report a novel platform with sustained release of minicircle DNA (MC) from PLGA scaffolds to accelerate bone repair. MC was encapsulated inside PLGA scaffolds using supercritical CO2 , which showed prolonged release of MC. Skull-derived osteoblasts transfected with BMP-2 MC in vitro result in higher osteocalcin gene expression and mineralized bone formation. When implanted in a critical-size mouse calvarial defect, scaffolds containing luciferase MC lead to robust in situ protein production up to at least 60 days. Scaffold-mediated BMP-2 MC delivery leads to substantially accelerated bone repair as early as two weeks, which continues to progress over 12 weeks. This platform represents an efficient, long-term nonviral gene delivery system, and may be applicable for enhancing repair of a broad range of tissues types. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2099-2107, 2016.
View details for DOI 10.1002/jbm.a.35735
View details for Web of Science ID 000379736500025
View details for PubMedID 27059085
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Swedish Medical Nanoscience Center at Karolinska Institutet
NANOTECHNOLOGY REVIEWS
2012; 1 (3): 273-279
View details for DOI 10.1515/ntrev-2012-0006
View details for Web of Science ID 000208982500005
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In Vivo Sustained Release of siRNA from Solid Lipid Nanoparticles
ACS NANO
2011; 5 (12): 9977-9983
Abstract
Small interfering RNA (siRNA) is a highly potent drug in gene-based therapy with a challenge of being delivered in a sustained manner. Nanoparticle drug delivery systems allow for incorporating and controlled release of therapeutic payloads. We demonstrate that solid lipid nanoparticles can incorporate and provide sustained release of siRNA. Tristearin solid lipid nanoparticles, made by nanoprecipitation, were loaded with siRNA (4.4-5.5 wt % loading ratio) using a hydrophobic ion pairing approach that employs the cationic lipid DOTAP. Intradermal injection of these nanocarriers in mouse footpads resulted in prolonged siRNA release over a period of 10-13 days. In vitro cell studies showed that the released siRNA retained its activity. Nanoparticles developed in this study offer an alternative approach to polymeric nanoparticles for encapsulation and sustained delivery of siRNA with the advantage of being prepared from physiologically well-tolerated materials.
View details for DOI 10.1021/nn203745n
View details for Web of Science ID 000298316700073
View details for PubMedID 22077198
View details for PubMedCentralID PMC3246574
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Biodegradable Nanoparticles With Sustained Release of Functional siRNA in Skin
JOURNAL OF PHARMACEUTICAL SCIENCES
2010; 99 (10): 4261-4266
Abstract
A key challenge in developing RNAi-based therapeutics is efficient delivery of functional short interfering RNA (siRNA) to target cells. To address this need, we have used a supercritical CO(2) process to incorporate siRNA in biodegradable polymer nanoparticles (NPs) for in vivo sustained release. By this means we have obtained complete encapsulation of the siRNA with minimal initial burst effect from the surface of the NPs. The slow release of a fluorescently labeled siRNA mimic (siGLO Red) was observed for up to 80 days in vivo after intradermal injection into mouse footpads. In vivo gene silencing experiments were also performed, showing reduction of GFP signal in the epidermis of a reporter transgenic mouse model, which demonstrates that the siRNA retained activity following release from the polymer NPs.
View details for DOI 10.1002/jps.22147
View details for Web of Science ID 000282473400012
View details for PubMedID 20737633
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Nanoparticle Formation of Organic Compounds With Retained Biological Activity
JOURNAL OF PHARMACEUTICAL SCIENCES
2010; 99 (6): 2750-2755
Abstract
Many pharmaceuticals are formulated as powders to aid drug delivery. A major problem is how to produce powders having high purity, controlled morphology, and retained bioactivity. We demonstrate the use of supercritical carbon dioxide as an antisolvent for meeting this need for two model drug systems, quercetin, a sparingly soluble antioxidant, and short interfering RNA (siRNA), which can silence genes. In both cases we achieve retention of bioactivity as well as a narrow particle size distribution in which the particles are free of impurities.
View details for DOI 10.1002/jps.22035
View details for Web of Science ID 000278241800022
View details for PubMedID 20039390
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Sustained release of nucleic acids from polymeric nanoparticles using microemulsion precipitation in supercritical carbon dioxide
CHEMICAL COMMUNICATIONS
2010; 46 (47): 9034-9036
Abstract
A general approach for producing biodegradable nanoparticles for sustained nucleic acid release is presented. The nanoparticles are produced by precipitating a water-in-oil microemulsion in supercritical CO(2). The microemulsion consists of a transfer RNA aqueous solution (water phase), dichloromethane containing poly(l-lactic acid)-poly(ethylene glycol) (oil phase), the surfactant n-octyl β-D-glucopyranoside, and the cosurfactant n-butanol.
View details for DOI 10.1039/c0cc04258g
View details for Web of Science ID 000284482100040
View details for PubMedID 21052588
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Sustained release of drugs dispersed in polymer nanoparticles
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
2008; 47 (41): 7880-7882
View details for DOI 10.1002/anie.200802260
View details for Web of Science ID 000260062500019
View details for PubMedID 18773393
View details for PubMedCentralID PMC2586612
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Subcritical water extraction and beta-glucosidase-catalyzed hydrolysis of quercetin glycosides in onion waste
GREEN CHEMISTRY
2006; 8 (11): 949-959
View details for DOI 10.1039/b608011a
View details for Web of Science ID 000241588000008
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Organic synthesis in water carbon dioxide emulsions
JOURNAL OF ORGANIC CHEMISTRY
1999; 64 (4): 1207-1210
View details for Web of Science ID 000078826200024
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Organic synthesis in water carbon dioxide microemulsions
JOURNAL OF ORGANIC CHEMISTRY
1999; 64 (4): 1201-1206
View details for Web of Science ID 000078826200023
- Biphasic Catalysis in Water/Carbon Dioxide Emulsions, Journal of the American Chemical Society 1999; 121 (50)
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Synthesis of O-[C-11]acetyl CoA, O-[C-11]acetyl-L-carnitine, L-[C-11]carnitine labelled in specific positions, applied in PET studies on rhesus monkey
NUCLEAR MEDICINE AND BIOLOGY
1997; 24 (5): 471-478
Abstract
The syntheses of L-carnitine, O-acetyl CoA, and O-acetyl-L-carnitine labelled with 11C at the 1- or 2-position of the acetyl group or the N-methyl position of carnitine, using the enzymes acetyl CoA synthetase and carnitine acetyltransferase, are described. With a total synthesis time of 45 min, O-[1-11C]acetyl CoA and O-[2[11C]acetyl CoA was obtained in 60-70% decay-corrected radiochemical yield, and O-[1-11C]acetyl-L-carnitine and O-[2-11C] acetyl-L-carnitine in 70-80% yield, based on [1-11C]acetate or [2-11C]acetate, respectively. By an N-methylation reaction with [11C]methyl iodide, L-[methyl-11C]carnitine was obtained within 30 min, and O-acetyl-L-[methyl-11C]carnitine within 40 min, giving a decay-corrected radiochemical yield of 60% and 40-50%, respectively, based on [11C]methyl iodide. Initial data of the kinetics of the different 11C-labelled L-carnitine and acetyl-L-carnitines in renal cortex of anaesthetized monkey (Macaca mulatta) are presented.
View details for Web of Science ID A1997XP97800017
View details for PubMedID 9290085
- Supercritical Fluid Synthesis and On-line Preparative Supercritical Fluid Chromatography of 11C-Labelled Compounds in Supercritical Ammonia. ACTA CHEM. SCAND. 1997; 51
- Synthesis of 11 C-Labeled Guanidines in Supercritical Ammonia JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1997; 118 (29): 6868
- High Uptake of [2-11C]Acetyl-l-Carnitine into the Brain: A Pet Study Biochemical and Biophysical Research Communications 1997: 488
- Supercritical Fluid Extraction of 11C-Labelled Metabolites from Tissue using Supercritical Ammonia. ANAL. CHEM. 1997
- Methodological aspects for in vitro characterization of receptor binding using 11C-Labelled receptor ligands: a detailed study with the benzodiazepine receptor antagonist [11C]Ro 15-1788 NUCL. MED. BIOL. 1997; 24 (8): 723
- Acylcarnitine and Chronic Fatigue Syndrome. CARNITINE TODAY 1997
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PET and parathyroid L-[carbon-11]methionine accumulation in hyperparathyroidism
JOURNAL OF NUCLEAR MEDICINE
1996; 37 (11): 1766-1770
Abstract
The study was designed to characterize L-[methyl-11 C]methionine accumulation in abnormal parathyroid tissues of hyperparathyroidism (HPT).Thirty-four patients with primary (n = 32) or secondary HPT were investigated with PET before primary or reoperative (n = 25) parathyroid surgery. Parathyroid 11C-methionine accumulation was analyzed for integrated uptake values in defined tissue volumes standardized for the injected dose and body weight (SUV), four contiguous pixels of maximal accumulation (SUVhs), SUV multiplied by area of region of interest (SUVr) and by the excised tissue weight (SUVw). Transport rate constants (slope, slopehs) were calculated according to Patlak's formula using plasma 11C activity corrected for 11C-methionine metabolites.True-positive localization was achieved in 85% of patients in whom 81% of the excised parathyroid lesions were visualized; no false-positive results were obtained. Corresponding proportions were 59% and 57% for CT and 55% and 52% for ultrasound, respectively. In the true-positive cases, parathyroid SUV, SUVhs and transport rate constants were consistently higher (p < 0.01) than in the thyroid, pharynx-esophagus, neck muscle and apical lung. Parathyroid SUV, SUVhs and SUVr increased with intact serum parathyroid hormone and calcium values (p = 0.0001-0.031), and weight of the excised tissue correlated with SUV and SUVhs (p = 0.024, 0.044). Parathyroid SUVhs varied strongly with the transport rate constants (p = 0.0008), and SUVr as well as s-calcium values differed significantly between parathyroid adenomas (n = 11), chief cell hyperplasias (n = 13), inadvertent implants (n = 3) and parathyroid cancers (n = 3).Carbon-11-methionine PET has potential application in preoperative localization and metabolic characterization of abnormal parathyroid tissues in human HPT.
View details for Web of Science ID A1996VR84600013
View details for PubMedID 8917171
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Levodopa-induced changes in synaptic dopamine in patients with Parkinson's disease as measured by [C-11]raclopride displacement and PET
NEUROLOGY
1996; 46 (5): 1430-1436
Abstract
Changes in striatal binding of [11C]raclopride, a dopamine D2 receptor antagonist, induced by acute levodopa administration, were evaluated with PET in 10 patients with idiopathic Parkinson's disease (PD). The patients were scanned on two occasions: drug-free and 15 minutes after a 5-minute intravenous infusion of 3 mg/kg levodopa. Levodopa administration produced reductions in striatal [11C]raclopride uptake index with a rostrocaudal gradient. The most pronounced reduction was found in the posterior putamen (to 82% of baseline), followed by the anterior putamen (to 88% of baseline) and the caudate nucleus (to 94% of baseline). The magnitude of [11C]raclopride uptake index reduction correlated with drug-free disability. Moreover, in four hemiparkinsonian patients, a reduction in [11C]raclopride uptake index was measured in the putamen contralateral to the parkinsonian symptoms. The present results demonstrate a positive correlation between striatal dopaminergic nerve-terminal deficiency and the capacity for levodopa to increase synaptic dopamine and displace [11C]raclopride binding, which corresponds to an accelerated amine turnover in dopamine-depleted striatal tissue. We therefore suggest that dopaminergic degeneration in PD is paralleled by a progressive acceleration of amine turnover. This mechanistic consequence of nigrostriatal degeneration, the selective restoration of synaptic dopaminergic neurotransmission in denervated striatal subregions, may explain the effectiveness of levodopa in producing symptomatic benefits in early PD. However, we also suggest that in the vastly denervated striatum, as in advanced PD, an excessive acceleration of amine turnover results in swings in levodopa-induced synaptic dopamine levels that are far beyond normal. This phenomenon most likely plays a key role in the pathogenesis underlying the development of motor-response complications in PD.
View details for Web of Science ID A1996UK62800043
View details for PubMedID 8628494
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IN-VITRO POSITRON EMISSION TOMOGRAPHY (PET) - USE OF POSITRON EMISSION TRACERS IN FUNCTIONAL IMAGING IN LIVING BRAIN-SLICES
NEUROSCIENCE RESEARCH
1995; 22 (2): 219-229
Abstract
Positron-emitting radionuclides have short half-lives and high radiation energies compared with radioisotopes generally used in biomedical research. We examined the possibility of applying positron emitter-labeled compounds to functional imaging in brain slices kept viable in an oxygenated buffer solution. Brain slices (300 microns thick) containing the striatum were incubated with positron emitter-labeled tracers for 30-45 min. The slices were then rinsed and placed on the bottom of a Plexiglas chamber filled with oxygenated Krebs-Ringer solution. The bottom of the chamber consisted of a thin polypropylene film to allow good penetration of beta+ particles from the brain slices. The chamber was placed on a storage phosphor screen, which has a higher sensitivity and a wider dynamic range than X-ray films. After an exposure period of 15-60 min, the screen was scanned by the analyzer and radioactivity images of brain slices were obtained within 20 min. We succeeded in obtaining quantitative images of (1) [18F]fluorodeoxyglucose uptake, (2) dopamine D2 receptor binding, (3) dopa-decarboxylase activity, and (4) release of [11C]dopamine preloaded as L-[11C]DOPA in the brain slice preparation. These results demonstrate that positron emitter-labeled tracers in combination with storage phosphor screens are useful for functional imaging of living brain slices as a novel neuroscience technique.
View details for Web of Science ID A1995RB42100008
View details for PubMedID 7566703
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Synthesis of C-11-labelled compounds in supercritical ammonia
5th International Symposium on Synthesis and Applications of Isotopically Labelled Compounds 1994
JOHN WILEY & SONS LTD. 1995: 343–346
View details for Web of Science ID A1995BD92K00059
- Synthesis of [11C]Acetyl-CoA and [11C]Acetyl-L-Carnitine. Synth. Appl. Isot. Labelled Compounds 1995; 118 (29): 375
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Synthesis of [C-11]acetyl-CoA and [C-11]acetyl-L-carnitine
5th International Symposium on Synthesis and Applications of Isotopically Labelled Compounds 1994
JOHN WILEY & SONS LTD. 1995: 375–377
View details for Web of Science ID A1995BD92K00067
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SUPERCRITICAL-FLUID SYNTHESIS IN THE PREPARATION OF BETA(+)-EMITTING LABELED COMPOUNDS
ACTA CHEMICA SCANDINAVICA
1994; 48 (5): 428-433
View details for Web of Science ID A1994NL02900011