Ian Guldner
Postdoctoral Scholar, Neurology and Neurological Sciences
All Publications
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Activated immune cells drive neurodegeneration in an Alzheimer's model.
Nature
2023; 615 (7953): 588-589
View details for DOI 10.1038/d41586-023-00600-5
View details for PubMedID 36890310
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Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes.
STAR protocols
2021; 2 (2): 100537
Abstract
High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries. High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling. Here, we describe a protocol for epitope and RNA-preserving dissociation of adult mouse brains and subsequent leukocyte purification and staining, which is adaptable to homeostatic and pathogenic brains. Leukocyte preparation following this protocol permits exquisite single-cell surface protein and RNA profiling in applications including CyTOF and CITE-seq. For complete details on the use and execution of this protocol, please refer to Guldner etal. (2020) and Golomb etal. (2020).
View details for DOI 10.1016/j.xpro.2021.100537
View details for PubMedID 34036283