Ines Forrest

All Publications

• Proteome-Wide Discovery of Degradable Proteins Using Bifunctional Molecules ACS CENTRAL SCIENCE Forrest, I., Conway, L. P., Gathmann, C., Jadhav, A. M., Chiu, T., Chaheine, C. M., Estrada, M., Shrestha, A., Sarris, K., Reitsma, J. M., Warder, S. E., Vasudevan, A., McLoughlin, S. M., Parker, C. G. 2025

  View details for DOI 10.1021/acscentsci.5c01594

  View details for Web of Science ID 001598175300001

• Proteome-Wide Fragment-Based Ligand and Target Discovery. Israel journal of chemistry Forrest, I., Parker, C. G. 2023; 63 (3-4)

  Abstract

  Chemical probes are invaluable tools to investigate biological processes and can serve as lead molecules for the development of new therapies. However, despite their utility, only a fraction of human proteins have selective chemical probes, and more generally, our knowledge of the "chemically-tractable" proteome is limited, leaving many potential therapeutic targets unexploited. To help address these challenges, powerful chemical proteomic approaches have recently been developed to globally survey the ability of proteins to bind small molecules (i. e., ligandability) directly in native systems. In this review, we discuss the utility of such approaches, with a focus on the integration of chemoproteomic methods with fragment-based ligand discovery (FBLD), to facilitate the broad mapping of the ligandable proteome while also providing starting points for progression into lead chemical probes.

  View details for DOI 10.1002/ijch.202200098

  View details for PubMedID 38213795

  View details for PubMedCentralID PMC10783656

• Proteome-Wide Discovery of Degradable Proteins Using Bifunctional Molecules bioRxiv Forrest, I., Conway, L. P., Jadhav, A. M., Gathmann, C., Chiu, T. Y., Estrada, M., Shrestha, A., Reitsma, J. M., Warder, S., Vasudevan, A., McLoughlin, S. M., Parker, C. G. 2025

  View details for DOI 10.1101/2025.03.21.644652

• Local Phenomena Shape Backyard Soil Metabolite Composition. Metabolites Nguyen, T. D., Lesani, M., Forrest, I., Lan, Y., Dean, D. A., Gibaut, Q. M., Guo, Y., Hossain, E., Olvera, M., Panlilio, H., Parab, A. R., Wu, C., Bernatchez, J. A., Cichewicz, R. H., McCall, L. I. 2020; 10 (3)

  Abstract

  Soil covers most of Earth's continental surface and is fundamental to life-sustaining processes such as agriculture. Given its rich biodiversity, soil is also a major source for natural product drug discovery from soil microorganisms. However, the study of the soil small molecule profile has been challenging due to the complexity and heterogeneity of this matrix. In this study, we implemented high-resolution liquid chromatography-tandem mass spectrometry and large-scale data analysis tools such as molecular networking to characterize the relative contributions of city, state and regional processes on backyard soil metabolite composition, in 188 soil samples collected from 14 USA States, representing five USA climate regions. We observed that region, state and city of collection all influence the overall soil metabolite profile. However, many metabolites were only detected in unique sites, indicating that uniquely local phenomena also influence the backyard soil environment, with both human-derived and naturally-produced (plant-derived, microbially-derived) metabolites identified. Overall, these findings are helping to define the processes that shape the backyard soil metabolite composition, while also highlighting the need for expanded metabolomic studies of this complex environment.

  View details for DOI 10.3390/metabo10030086

  View details for PubMedID 32121389

  View details for PubMedCentralID PMC7143036