Professional Education


  • Master of Science, Umea Universitet (2010)
  • Doctor of Philosophy, Karolinska Institutet (2017)
  • Master of Science in Engr, Umea Universitet (2010)

Stanford Advisors


All Publications


  • A Widespread Neurogenic Potential of Neocortical Astrocytes Is Induced by Injury CELL STEM CELL Zamboni, M., Llorens-Bobadilla, E., Magnusson, J., Frisen, J. 2020; 27 (4): 605-+

    Abstract

    Parenchymal astrocytes have emerged as a potential reservoir for new neurons in non-neurogenic brain regions. It is currently unclear how astrocyte neurogenesis is controlled molecularly. Here we show that Notch signaling-deficient astrocytes can generate new neurons after injury. Using single-cell RNA sequencing, we found that, when Notch signaling is blocked, astrocytes transition to a neural stem cell-like state. However, only after injury do a few of these primed astrocytes unfold a neurogenic program, including a self-amplifying progenitor-like state. Further, reconstruction of the trajectories of individual cells allowed us to uncouple astrocyte neurogenesis from reactive gliosis, which occur along independent branches. Finally, we show that cortical neurogenesis molecularly recapitulates canonical subventricular zone neurogenesis with remarkable fidelity. Our study supports a widespread potential of parenchymal astrocytes to function as dormant neural stem cells.

    View details for DOI 10.1016/j.stem.2020.07.006

    View details for Web of Science ID 000577245400014

    View details for PubMedID 32758425

    View details for PubMedCentralID PMC7534841

  • Activation of a neural stem cell transcriptional program in parenchymal astrocytes. eLife Magnusson, J. P., Zamboni, M., Santopolo, G., Mold, J. E., Barrientos-Somarribas, M., Talavera-Lopez, C., Andersson, B., Frisen, J. 2020; 9

    Abstract

    Adult neural stem cells, located in discrete brain regions, generate new neurons throughout life. These stem cells are specialized astrocytes, but astrocytes in other brain regions do not generate neurons under physiological conditions. After stroke, however, striatal astrocytes undergo neurogenesis in mice, triggered by decreased Notch signaling. We used single-cell RNA sequencing to characterize neurogenesis by Notch-depleted striatal astrocytes in vivo. Striatal astrocytes were located upstream of neural stem cells in the neuronal lineage. As astrocytes initiated neurogenesis, they became transcriptionally very similar to subventricular zone stem cells, progressing through a near-identical neurogenic program. Surprisingly, in the non-neurogenic cortex, Notch-depleted astrocytes also initiated neurogenesis. Yet, these cortical astrocytes, and many striatal ones, stalled before entering transit-amplifying divisions. Infusion of epidermal growth factor enabled stalled striatal astrocytes to resume neurogenesis. We conclude that parenchymal astrocytes are latent neural stem cells and that targeted interventions can guide them through their neuronal differentiation.

    View details for DOI 10.7554/eLife.59733

    View details for PubMedID 32744501

  • Blocking Notch-Signaling Increases Neurogenesis in the Striatum after Stroke CELLS Santopolo, G., Magnusson, J. P., Lindvall, O., Kokaia, Z., Frisen, J. 2020; 9 (7)

    Abstract

    Stroke triggers neurogenesis in the striatum in mice, with new neurons deriving in part from the nearby subventricular zone and in part from parenchymal astrocytes. The initiation of neurogenesis by astrocytes within the striatum is triggered by reduced Notch-signaling, and blocking this signaling pathway by deletion of the gene encoding the obligate Notch coactivator Rbpj is sufficient to activate neurogenesis by striatal astrocytes in the absence of an injury. Here we report that blocking Notch-signaling in stroke increases the neurogenic response to stroke 3.5-fold in mice. Deletion of Rbpj results in the recruitment of a larger number of parenchymal astrocytes to neurogenesis and over larger areas of the striatum. These data suggest inhibition of Notch-signaling as a potential translational strategy to promote neuronal regeneration after stroke.

    View details for DOI 10.3390/cells9071732

    View details for Web of Science ID 000554092400001

    View details for PubMedID 32698472

  • Stars from the darkest night: unlocking the neurogenic potential of astrocytes in different brain regions. Development (Cambridge, England) Magnusson, J. P., Frisén, J. n. 2016; 143 (7): 1075–86

    Abstract

    In a few regions of the adult brain, specialized astrocytes act as neural stem cells capable of sustaining life-long neurogenesis. In other, typically non-neurogenic regions, some astrocytes have an intrinsic capacity to produce neurons when provoked by particular conditions but do not use this ability to replace neurons completely after injury or disease. Why do astrocytes display regional differences and why do they not use their neurogenic capacity for brain repair to a greater extent? In this Review, we discuss the neurogenic potential of astrocytes in different brain regions and ask what stimulates this potential in some regions but not in others. We discuss the transcriptional networks and environmental cues that govern cell identity, and consider how the activation of neurogenic properties in astrocytes can be understood as the de-repression of a latent neurogenic transcriptional program.

    View details for DOI 10.1242/dev.133975

    View details for PubMedID 27048686

  • A latent neurogenic program in astrocytes regulated by Notch signaling in the mouse. Science (New York, N.Y.) Magnusson, J. P., Göritz, C. n., Tatarishvili, J. n., Dias, D. O., Smith, E. M., Lindvall, O. n., Kokaia, Z. n., Frisén, J. n. 2014; 346 (6206): 237–41

    Abstract

    Neurogenesis is restricted in the adult mammalian brain; most neurons are neither exchanged during normal life nor replaced in pathological situations. We report that stroke elicits a latent neurogenic program in striatal astrocytes in mice. Notch1 signaling is reduced in astrocytes after stroke, and attenuated Notch1 signaling is necessary for neurogenesis by striatal astrocytes. Blocking Notch signaling triggers astrocytes in the striatum and the medial cortex to enter a neurogenic program, even in the absence of stroke, resulting in 850 ± 210 (mean ± SEM) new neurons in a mouse striatum. Thus, under Notch signaling regulation, astrocytes in the adult mouse brain parenchyma carry a latent neurogenic program that may potentially be useful for neuronal replacement strategies.

    View details for DOI 10.1126/science.346.6206.237

    View details for PubMedID 25301628