Honors & Awards
Berg Scholar, Stanford University School of Medicine (2022-2025)
Education & Certifications
Master of Science, Stanford University, Bioengineering (2019)
Bachelor of Arts, Harvard College, Biomedical Engineering (2017)
Large-Scale Production of Wholly-Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors.
Advanced healthcare materials
Combining the sustainable culture of billions of human cells and the bioprinting of wholly-cellular bioinks offers a pathway towards organ-scale tissue engineering. Traditional 2D culture methods are not inherently scalable due to cost, space, and handling constraints. Here, we optimize the suspension culture of human induced pluripotent stem cell-derived aggregates using an automated 250 mL stirred tank bioreactor system. Cell yield, aggregate morphology, and pluripotency marker expression are maintained over three serial passages in two distinct cell lines. Furthermore, we demonstrate that the same optimized parameters can be scaled to an automated 1 L stirred tank bioreactor system. Our 4-day culture resulted in a 16.6- to 20.4-fold expansion of cells, we generate approximately 4 billion cells per vessel, while maintaining > 94% expression of pluripotency markers. The pluripotent aggregates can be subsequently differentiated into derivatives of the three germ layers, including cardiac aggregates, and vascular, cortical and intestinal organoids. Finally, the aggregates are compacted into a wholly-cellular bioink for rheological characterization and 3D bioprinting. The printed hAs are subsequently differentiated into neuronal and vascular tissue. This work demonstrates an optimized suspension culture-to-3D bioprinting pipeline that enables a sustainable approach to billion cell-scale organ engineering. This article is protected by copyright. All rights reserved.
View details for DOI 10.1002/adhm.202201138
View details for PubMedID 36314397
Exercise Capacity and Training Programs in Pediatric Fontan Patients: A Systematic Review
CJC Pediatric and Congenital Heart Disease
2022; 1 (3)
View details for DOI 10.1016/j.cjcpc.2022.04.005
Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips
2016; 6: 34845
Three-dimensional models of kidney tissue that recapitulate human responses are needed for drug screening, disease modeling, and, ultimately, kidney organ engineering. Here, we report a bioprinting method for creating 3D human renal proximal tubules in vitro that are fully embedded within an extracellular matrix and housed in perfusable tissue chips, allowing them to be maintained for greater than two months. Their convoluted tubular architecture is circumscribed by proximal tubule epithelial cells and actively perfused through the open lumen. These engineered 3D proximal tubules on chip exhibit significantly enhanced epithelial morphology and functional properties relative to the same cells grown on 2D controls with or without perfusion. Upon introducing the nephrotoxin, Cyclosporine A, the epithelial barrier is disrupted in a dose-dependent manner. Our bioprinting method provides a new route for programmably fabricating advanced human kidney tissue models on demand.
View details for DOI 10.1038/srep34845
View details for Web of Science ID 000385242900001
View details for PubMedID 27725720
View details for PubMedCentralID PMC5057112