
John Ming Lu
MD Student, expected graduation Spring 2025
Ph.D. Student in Stem Cell Biology and Regenerative Medicine, admitted Autumn 2022
MSTP Student
All Publications
-
Multi-Modal Analysis of Cell Populations and Architectural States Mediating the Progression and Resolution of Pulmonary Fibrosis
LIPPINCOTT WILLIAMS & WILKINS. 2022: S82
View details for Web of Science ID 000867877000204
-
Structures and distributions of SARS-CoV-2 spike proteins on intact virions
NATURE
2020
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virions are surrounded by a lipid bilayer from which spike (S) protein trimers protrude1. Heavily glycosylated S trimers bind to the angiotensin-converting enzyme 2 receptor and mediate entry of virions into target cells2-6. S exhibits extensive conformational flexibility: it modulates exposure of its receptor-binding site and subsequently undergoes complete structural rearrangement to drive fusion of viral and cellular membranes2,7,8. The structures and conformations of soluble, overexpressed, purified S proteins have been studied in detail using cryo-electron microscopy2,7,9-12, but the structure and distribution of S on the virion surface remain unknown. Here we applied cryo-electron microscopy and tomography to image intact SARS-CoV-2 virions and determine the high-resolution structure, conformational flexibility and distribution of S trimers in situ on the virion surface. These results reveal the conformations of S on the virion, and provide a basis from which to understand interactions between S and neutralizing antibodies during infection or vaccination.
View details for DOI 10.1038/s41586-020-2665-2
View details for Web of Science ID 000585787300001
View details for PubMedID 32805734